LIVIVO - Search results -

Search results

Result 1 - 10 of total 15

Search options

Article: Data of sperm-entry inability in Drosophila melanogaster ovarian follicles that are depleted of s36 chorionic protein

Velentzas, Athanassios D. / Velentzas, Panagiotis D. / Katarachia, Stamatia / Mpakou, Vassiliki E. / Papassideri, Issidora S. / Stravopodis, Dimitrios J.

Data in Brief. 2017 June, v. 12

2017

Abstract: This paper presents data associated with the research article entitled “Targeted downregulation of s36 protein unearths its cardinal role in chorion biogenesis and architecture during Drosophila melanogaster oogenesis” [1]. Drosophila chorion is produced ...

Abstract	This paper presents data associated with the research article entitled “Targeted downregulation of s36 protein unearths its cardinal role in chorion biogenesis and architecture during Drosophila melanogaster oogenesis” [1]. Drosophila chorion is produced by epithelial follicle cells and one of its functional serving role is egg fertilization through the micropyle, a specialized narrow channel at the anterior tip of the egg [2]. Sperm entry during fertilization is necessary for the egg to complete meiosis [3]. D. melanogaster flies being characterized by severe downregulation of the s36 chorionic protein, specifically in the follicle-cell compartment of their ovary, appear with impaired fly fertility (Velentzas et al., 2016) [1]. In an effort to further investigate whether the observed infertility in the s36-targeted flies derives from a fertilization failure, such as the inability of sperm to pass through egg׳s micropyle, we mated females carrying s36-depleted ovaries with males expressing the GFP protein either in their sperm tails, or in both their sperm tails and sperm heads.
Keywords	Drosophila melanogaster ; biogenesis ; chorion ; eggs ; epithelium ; meiosis ; oogenesis ; spermatozoa
Language	English
Dates of publication	2017-06
Size	p. 180-183.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2017.03.052
Database	NAL-Catalogue (AGRICOLA)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Proteomic mapping of Drosophila transgenic elav.L-GAL4/+ brain as a tool to illuminate neuropathology mechanisms.

Velentzas, Athanassios D / Katarachia, Stamatia A / Sagioglou, Niki E / Tsioka, Maria M / Anagnostopoulos, Athanasios K / Mpakou, Vassiliki E / Theotoki, Eleni I / Giannopoulou, Aikaterini F / Keramaris, Konstantinos E / Papassideri, Issidora S / Tsangaris, George Th / Stravopodis, Dimitrios J

Scientific reports

2020 Volume 10, Issue 1, Page(s) 5430

Abstract: Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies. To map the proteomic landscape of fly brain, in a high-resolution scale, we herein employed a nano liquid chromatography- ... ...

Abstract	Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies. To map the proteomic landscape of fly brain, in a high-resolution scale, we herein employed a nano liquid chromatography-tandem mass spectrometry technology, and high-content catalogues of 7,663 unique peptides and 2,335 single proteins were generated. Protein-data processing, through UniProt, DAVID, KEGG and PANTHER bioinformatics subroutines, led to fly brain-protein classification, according to sub-cellular topology, molecular function, implication in signaling and contribution to neuronal diseases. Given the importance of Ubiquitin Proteasome System (UPS) in neuropathologies and by using the almost completely reassembled UPS, we genetically targeted genes encoding components of the ubiquitination-dependent protein-degradation machinery. This analysis showed that driving RNAi toward proteasome components and regulators, using the GAL4-elav.L driver, resulted in changes to longevity and climbing-activity patterns during aging. Our proteomic map is expected to advance the existing knowledge regarding brain biology in animal species of major translational-research value and economical interest.
MeSH term(s)	Animals ; Animals, Genetically Modified ; Brain/metabolism ; Drosophila/genetics ; Drosophila/physiology ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; ELAV Proteins/genetics ; Female ; Humans ; Locomotion/genetics ; Longevity/genetics ; Male ; Nervous System Diseases/etiology ; Nervous System Diseases/genetics ; Proteasome Endopeptidase Complex/genetics ; Proteolysis ; Proteomics/methods ; RNA Interference ; Transcription Factors/genetics ; Ubiquitin/metabolism ; Ubiquitination/genetics
Chemical Substances	Drosophila Proteins ; ELAV Proteins ; ELAV protein, Drosophila ; GAL4 protein, Drosophila ; Transcription Factors ; Ubiquitin ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2020-03-25
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-62510-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Data of sperm-entry inability in

Velentzas, Athanassios D / Velentzas, Panagiotis D / Katarachia, Stamatia / Mpakou, Vassiliki E / Papassideri, Issidora S / Stravopodis, Dimitrios J

Data in brief

2017 Volume 12, Page(s) 180–183

Abstract: This paper presents data associated with the research article entitled "Targeted downregulation of s36 protein unearths its cardinal role in chorion biogenesis and architecture ... ...

Abstract	This paper presents data associated with the research article entitled "Targeted downregulation of s36 protein unearths its cardinal role in chorion biogenesis and architecture during
Language	English
Publishing date	2017-04-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2017.03.052
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Revisiting Histone Deacetylases in Human Tumorigenesis: The Paradigm of Urothelial Bladder Cancer.

Giannopoulou, Aikaterini F / Velentzas, Athanassios D / Konstantakou, Eumorphia G / Avgeris, Margaritis / Katarachia, Stamatia A / Papandreou, Nikos C / Kalavros, Nikolas I / Mpakou, Vassiliki E / Iconomidou, Vassiliki / Anastasiadou, Ema / Kostakis, Ioannis K / Papassideri, Issidora S / Voutsinas, Gerassimos E / Scorilas, Andreas / Stravopodis, Dimitrios J

International journal of molecular sciences

2019 Volume 20, Issue 6

Abstract: Urinary bladder cancer is a common malignancy, being characterized by substantial patient mortality and management cost. Its high somatic-mutation frequency and molecular heterogeneity usually renders tumors refractory to the applied regimens. Hitherto, ... ...

Abstract	Urinary bladder cancer is a common malignancy, being characterized by substantial patient mortality and management cost. Its high somatic-mutation frequency and molecular heterogeneity usually renders tumors refractory to the applied regimens. Hitherto, methotrexate-vinblastine-adriamycin-cisplatin and gemcitabine-cisplatin represent the backbone of systemic chemotherapy. However, despite the initial chemosensitivity, the majority of treated patients will eventually develop chemoresistance, which severely reduces their survival expectancy. Since chromatin regulation genes are more frequently mutated in muscle-invasive bladder cancer, as compared to other epithelial tumors, targeted therapies against chromatin aberrations in chemoresistant clones may prove beneficial for the disease. "Acetyl-chromatin" homeostasis is regulated by the opposing functions of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDAC/SIRT (super-)family contains 18 members, which are divided in five classes, with each family member being differentially expressed in normal urinary bladder tissues. Since a strong association between irregular HDAC expression/activity and tumorigenesis has been previously demonstrated, we herein attempt to review the accumulated published evidences that implicate HDACs/SIRTs as critical regulators in urothelial bladder cancer. Moreover, the most extensively investigated HDAC inhibitors (HDACis) are also analyzed, and the respective clinical trials are also described. Interestingly, it seems that HDACis should be preferably used in drug-combination therapeutic schemes, including radiation.
MeSH term(s)	Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Carcinoma, Transitional Cell/drug therapy ; Carcinoma, Transitional Cell/enzymology ; Chromatin Assembly and Disassembly/drug effects ; Clinical Trials as Topic ; Drug Resistance, Neoplasm/drug effects ; Histone Deacetylase Inhibitors/chemistry ; Histone Deacetylase Inhibitors/pharmacology ; Histone Deacetylase Inhibitors/therapeutic use ; Histone Deacetylases/metabolism ; Humans ; Urinary Bladder Neoplasms/drug therapy ; Urinary Bladder Neoplasms/enzymology
Chemical Substances	Histone Deacetylase Inhibitors ; Histone Deacetylases (EC 3.5.1.98)
Language	English
Publishing date	2019-03-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms20061291
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster.

Velentzas, Athanassios D / Velentzas, Panagiotis D / Katarachia, Stamatia A / Anagnostopoulos, Athanasios K / Sagioglou, Niki E / Thanou, Eleni V / Tsioka, Maria M / Mpakou, Vassiliki E / Kollia, Zoe / Gavriil, Vassilios E / Papassideri, Issidora S / Tsangaris, George Th / Cefalas, Alkiviadis-Constantinos / Sarantopoulou, Evangelia / Stravopodis, Dimitrios J

Scientific reports

2018 Volume 8, Issue 1, Page(s) 16103

Abstract: Drosophila chorion represents a remarkable model system for the in vivo study of complex extracellular-matrix architectures. For its organization and structure, s38 protein is considered as a component of major importance, since it is synthesized and ... ...

Abstract	Drosophila chorion represents a remarkable model system for the in vivo study of complex extracellular-matrix architectures. For its organization and structure, s38 protein is considered as a component of major importance, since it is synthesized and secreted during early choriogenesis. However, there is no evidence that proves its essential, or redundant, role in chorion biogenesis. Hence, we show that targeted downregulation of s38 protein, specifically in the ovarian follicle-cell compartment, via employment of an RNAi-mediated strategy, causes generation of diverse dysmorphic phenotypes, regarding eggshell's regionally and radially specialized structures. Downregulation of s38 protein severely impairs fly's fertility and is unable to be compensated by the s36 homologous family member, thus unveiling s38 protein's essential contribution to chorion's assembly and function. Altogether, s38 acts as a key skeletal protein being critically implicated in the patterning establishment of a highly structured tripartite endochorion. Furthermore, it seems that s38 loss may sensitize choriogenesis to stochastic variation in its coordination and timing.
MeSH term(s)	Animals ; Cell Compartmentation ; Chorion/metabolism ; Down-Regulation ; Drosophila Proteins/metabolism ; Drosophila melanogaster/genetics ; Drosophila melanogaster/growth & development ; Drosophila melanogaster/metabolism ; Drosophila melanogaster/ultrastructure ; Egg Proteins/metabolism ; Egg Shell/cytology ; Egg Shell/metabolism ; Egg Shell/ultrastructure ; Female ; Fertility ; Gene Expression Regulation ; Morphogenesis ; Ovarian Follicle/cytology ; Ovarian Follicle/metabolism ; Ovum/metabolism ; RNA Interference
Chemical Substances	Cp38 protein, Drosophila ; Drosophila Proteins ; Egg Proteins
Language	English
Publishing date	2018-10-31
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-018-34532-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Proteasome, but not autophagy, disruption results in severe eye and wing dysmorphia: a subunit- and regulator-dependent process in Drosophila.

Velentzas, Panagiotis D / Velentzas, Athanassios D / Pantazi, Asimina D / Mpakou, Vassiliki E / Zervas, Christos G / Papassideri, Issidora S / Stravopodis, Dimitrios J

PloS one

2013 Volume 8, Issue 11, Page(s) e80530

Abstract: Proteasome-dependent and autophagy-mediated degradation of eukaryotic cellular proteins represent the two major proteostatic mechanisms that are critically implicated in a number of signaling pathways and cellular processes. Deregulation of functions ... ...

Abstract	Proteasome-dependent and autophagy-mediated degradation of eukaryotic cellular proteins represent the two major proteostatic mechanisms that are critically implicated in a number of signaling pathways and cellular processes. Deregulation of functions engaged in protein elimination frequently leads to development of morbid states and diseases. In this context, and through the utilization of GAL4/UAS genetic tool, we herein examined the in vivo contribution of proteasome and autophagy systems in Drosophila eye and wing morphogenesis. By exploiting the ability of GAL4-ninaE. GMR and P{GawB}Bx(MS1096) genetic drivers to be strongly and preferentially expressed in the eye and wing discs, respectively, we proved that proteasomal integrity and ubiquitination proficiency essentially control fly's eye and wing development. Indeed, subunit- and regulator-specific patterns of severe organ dysmorphia were obtained after the RNAi-induced downregulation of critical proteasome components (Rpn1, Rpn2, α5, β5 and β6) or distinct protein-ubiquitin conjugators (UbcD6, but not UbcD1 and UbcD4). Proteasome deficient eyes presented with either rough phenotypes or strongly dysmorphic shapes, while transgenic mutant wings were severely folded and carried blistered structures together with loss of vein differentiation. Moreover, transgenic fly eyes overexpressing the UBP2-yeast deubiquitinase enzyme were characterized by an eyeless-like phenotype. Therefore, the proteasome/ubiquitin proteolytic activities are undoubtedly required for the normal course of eye and wing development. In contrast, the RNAi-mediated downregulation of critical Atg (1, 4, 7, 9 and 18) autophagic proteins revealed their non-essential, or redundant, functional roles in Drosophila eye and wing formation under physiological growth conditions, since their reduced expression levels could only marginally disturb wing's, but not eye's, morphogenetic organization and architecture. However, Atg9 proved indispensable for the maintenance of structural integrity of adult wings in aged flies. In toto, our findings clearly demonstrate the gene-specific fundamental contribution of proteasome, but not autophagy, in invertebrate eye and wing organ development.
MeSH term(s)	Animals ; Autophagy ; Down-Regulation ; Drosophila melanogaster ; Eye Abnormalities/enzymology ; Microscopy, Electron, Scanning ; Proteasome Endopeptidase Complex/metabolism ; Ubiquitin/metabolism ; Wings, Animal/abnormalities
Chemical Substances	Ubiquitin ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2013-11-25
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0080530
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Detrimental effects of proteasome inhibition activity in Drosophila melanogaster: implication of ER stress, autophagy, and apoptosis.

Velentzas, Panagiotis D / Velentzas, Athanassios D / Mpakou, Vassiliki E / Antonelou, Marianna H / Margaritis, Lukas H / Papassideri, Issidora S / Stravopodis, Dimitrios J

Cell biology and toxicology

2013 Volume 29, Issue 1, Page(s) 13–37

Abstract: In eukaryotes, the ubiquitin-proteasome machinery regulates a number of fundamental cellular processes through accurate and tightly controlled protein degradation pathways. We have, herein, examined the effects of proteasome functional disruption in ... ...

Abstract	In eukaryotes, the ubiquitin-proteasome machinery regulates a number of fundamental cellular processes through accurate and tightly controlled protein degradation pathways. We have, herein, examined the effects of proteasome functional disruption in Dmp53 (+/+) (wild-type) and Dmp53 (-/-) Drosophila melanogaster fly strains through utilization of Bortezomib, a proteasome-specific inhibitor. We report that proteasome inhibition drastically shortens fly life-span and impairs climbing performance, while it also causes larval lethality and activates developmentally irregular cell death programs during oogenesis. Interestingly, Dmp53 gene seems to play a role in fly longevity and climbing ability. Moreover, Bortezomib proved to induce endoplasmic reticulum (ER) stress that was able to result in the engagement of unfolded protein response (UPR) signaling pathway, as respectively indicated by fly Xbp1 activation and Ref(2)P-containing protein aggregate formation. Larva salivary gland and adult brain both underwent strong ER stress in response to Bortezomib, thus underscoring the detrimental role of proteasome inhibition in larval development and brain function. We also propose that the observed upregulation of autophagy operates as a protective mechanism to "counterbalance" Bortezomib-induced systemic toxicity, which is tightly associated, besides ER stress, with activation of apoptosis, mainly mediated by functional Drice caspase and deregulated dAkt kinase. The reduced life-span of exposed to Bortezomib flies overexpressing Atg1_RNAi or Atg18_RNAi supports the protective nature of autophagy against proteasome inhibition-induced stress. Our data reveal the in vivo significance of proteasome functional integrity as a major defensive system against cellular toxicity likely occurring during critical biological processes and morphogenetic courses.
MeSH term(s)	Animals ; Animals, Genetically Modified ; Apoptosis/drug effects ; Apoptosis/physiology ; Autophagy/drug effects ; Autophagy/physiology ; Behavior, Animal/drug effects ; Boronic Acids/toxicity ; Bortezomib ; Drosophila melanogaster/drug effects ; Drosophila melanogaster/enzymology ; Endoplasmic Reticulum Stress/drug effects ; Endoplasmic Reticulum Stress/physiology ; Female ; Kaplan-Meier Estimate ; Larva/drug effects ; Larva/growth & development ; Longevity/drug effects ; Male ; Motor Activity/drug effects ; Ovarian Follicle/drug effects ; Ovarian Follicle/metabolism ; Proteasome Endopeptidase Complex/drug effects ; Proteasome Endopeptidase Complex/metabolism ; Proteasome Inhibitors/toxicity ; Pyrazines/toxicity ; Survival Rate
Chemical Substances	Boronic Acids ; Proteasome Inhibitors ; Pyrazines ; Bortezomib (69G8BD63PP) ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2013-02
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	48824-0
ISSN	1573-6822 ; 0742-2091
ISSN (online)	1573-6822
ISSN	0742-2091
DOI	10.1007/s10565-012-9235-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 3024: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Quantitative and qualitative analysis of regulatory T cells in B cell chronic lymphocytic leukemia.

Mpakou, Vassiliki E / Ioannidou, Heleni-Dikaia / Konsta, Eugene / Vikentiou, Myrofora / Spathis, Aris / Kontsioti, Frieda / Kontos, Christos K / Velentzas, Athanassios D / Papageorgiou, Sotiris / Vasilatou, Diamantina / Gkontopoulos, Konstantinos / Glezou, Irene / Stavroulaki, Georgia / Mpazani, Efthimia / Kokkori, Stella / Kyriakou, Elias / Karakitsos, Petros / Dimitriadis, George / Pappa, Vasiliki

Leukemia research

2017 Volume 60, Page(s) 74–81

Abstract: Accumulated data indicate a significant role of T cell dysfunction in the pathogenesis of chronic lymphocytic leukemia. In CLL, regulatory T cells are significantly higher and show lower apoptotic levels compared to healthy donors. We demonstrate that ... ...

Abstract	Accumulated data indicate a significant role of T cell dysfunction in the pathogenesis of chronic lymphocytic leukemia. In CLL, regulatory T cells are significantly higher and show lower apoptotic levels compared to healthy donors. We demonstrate that CLL derived CD4
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Apoptosis ; CD4 Lymphocyte Count ; Cell Proliferation ; Female ; Humans ; Immunophenotyping ; Leukemia, Lymphocytic, Chronic, B-Cell/immunology ; Leukemia, Lymphocytic, Chronic, B-Cell/pathology ; Male ; Middle Aged ; T-Lymphocytes, Regulatory
Language	English
Publishing date	2017-09
Publishing country	England
Document type	Journal Article
ZDB-ID	752396-8
ISSN	1873-5835 ; 0145-2126
ISSN (online)	1873-5835
ISSN	0145-2126
DOI	10.1016/j.leukres.2017.07.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1321: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Global Proteomic Profiling of Drosophila Ovary: A High-resolution, Unbiased, Accurate and Multifaceted Analysis.

Velentzas, Athanassios D / Anagnostopoulos, Athanasios K / Velentzas, Panagiotis D / Mpakou, Vassiliki E / Sagioglou, Niki E / Tsioka, Maria M / Katarachia, Stamatia / Manta, Areti K / Konstantakou, Eumorphia G / Papassideri, Issidora S / Tsangaris, George T H / Stravopodis, Dimitrios J

Cancer genomics & proteomics

2015 Volume 12, Issue 6, Page(s) 369–384

Abstract: Background: Drosophila melanogaster ovary serves as an attractive model system for the investigation of the cell cycle, death, signaling, migration, differentiation, development and stemness. By employing the 3750/+ heterozygote fly strain that carries ... ...

Abstract	Background: Drosophila melanogaster ovary serves as an attractive model system for the investigation of the cell cycle, death, signaling, migration, differentiation, development and stemness. By employing the 3750/+ heterozygote fly strain that carries specific functions in the follicle cell compartment, and a reliable control in GAL4/UAS-based transgenic technology, we herein characterized the protein-expression profiling of D. melanogaster ovary by applying high-resolution proteomic tools and bioinformatics programs. Materials and methods: Whole-cell total protein extracts derived from 3750/+ fly ovaries were prepared under highly denaturing conditions and after tryptic digestion, their cognate peptides were processed to liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis in a high-resolution LTQ Orbitrap Elite instrument. Obtained protein data were analyzed through use of UniProt, DAVID, KEGG and PANTHER bioinformatics platforms. Results: The 7,583 unique peptides identified show that fly ovary contains at least 2,103 single proteins, which are distributed to all egg chamber compartments, in cytoplasm, membrane and nucleus, compartmentalized into major cellular organelles, and categorized into critical macromolecular assemblies. Among the recognized specific functions, nucleic acid binding, hydrolase, oxidoreductase, transporter and vesicle-mediated trafficking activities were the most prevalent. Determinants implicated in cellular metabolism and gene expression are represented by ~41% and ~17% of the ovarian proteome, respectively. Surprisingly, several proteins were found engaged in aging, immune response and neurogenesis. All major signaling pathways were detected, while apoptotic and non-apoptotic cell death programs were also identified. Remarkably, proteins involved in tumor formation, neurodegenerative and inflammatory diseases were also recognized. The successful remodeling of the proteasome and nearly complete molecular reconstruction of the citrate cycle and fatty acid degradation pathways demonstrate the efficacy, accuracy and fidelity of our combined proteomics/bioinformatics approach. Conclusion: Global proteomic characterization of D. melanogaster ovary allows the discovery of novel regulators and pathways, and provides a systemic view of networks that govern ovarian pathophysiology and embryonic development in fly species as well in humans.
MeSH term(s)	Animals ; Apoptosis ; Cell Cycle ; Cell Differentiation ; Cell Movement ; Chromatography, Liquid ; Computational Biology ; Drosophila melanogaster ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Heterozygote ; Inflammation ; Ovary/metabolism ; Proteome ; Proteomics ; Signal Transduction ; Tandem Mass Spectrometry
Chemical Substances	Proteome
Language	English
Publishing date	2015-11
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2144517-5
ISSN	1790-6245 ; 1109-6535
ISSN (online)	1790-6245
ISSN	1109-6535
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5873: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Programmed cell death of the ovarian nurse cells during oogenesis of the ladybird beetle Adalia bipunctata (Coleoptera: Coccinellidae).

Mpakou, Vassiliki E / Velentzas, Athanassios D / Velentzas, Panagiotis D / Margaritis, Lukas H / Stravopodis, Dimitrios J / Papassideri, Issidora S

Development, growth & differentiation

2011 Volume 53, Issue 6, Page(s) 804–815

Abstract: Programmed cell death (PCD) is an evolutionary conserved and genetically regulated form of cell death, in which the cell plays an active role in its own demise. It is widely recognized that PCD can be morphologically classified into three major types: ... ...

Abstract	Programmed cell death (PCD) is an evolutionary conserved and genetically regulated form of cell death, in which the cell plays an active role in its own demise. It is widely recognized that PCD can be morphologically classified into three major types: type I, known as apoptosis, type II, called autophagy, and type III, specified as cytoplasmic cell death. So far, PCD has been morphologically analyzed in certain model insect species of the meroistic polytrophic ovary-type, but has never been examined before in insects carrying meroistic telotrophic ovaries. In the present study, we attempted to thoroughly describe the three different types (I, II and III) of PCD occurring during oogenesis in the meroistic telotrophic ovary of the Coleoptera species Adalia bipunctata, at different developmental ages of the adult female insects. We reveal that in the ladybird beetle A. bipunctata, the ovarian tropharia undergo age-dependent forms of apoptotic, autophagic and cytoplasmic (paraptotic-like) cell death, which seem to operate in a rather synergistic fashion, in accordance with previous observations in Diptera and Lepidoptera species. Furthermore, we herein demonstrate the occurrence of morphogenetically abnormal ovarioles in A. bipunctata female insects. These atretic ovarioles collapse and die through a PCD-mediated process that is characterized by the combined activation of all three types of PCD. Conclusively, the distinct cell death programs (I, II and III) specifically engaged during oogenesis of A. bipunctata provide strong evidence for the structural and functional conserved nature of PCD during insect evolution among meroistic telotrophic and meroistic polytrophic ovary-type insects.
MeSH term(s)	Age Factors ; Animals ; Apoptosis ; Autophagy ; Cell Nucleus/ultrastructure ; Chromatin/physiology ; Coleoptera/anatomy & histology ; Coleoptera/cytology ; Coleoptera/physiology ; Cytoplasm/pathology ; Cytoplasm/physiology ; Female ; Microscopy, Electron, Transmission ; Oogenesis ; Ovary/cytology ; Ovary/pathology ; Ovary/ultrastructure ; Species Specificity ; Staining and Labeling
Chemical Substances	Chromatin
Language	English
Publishing date	2011-08
Publishing country	Japan
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1111/j.1440-169X.2011.01288.x
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 194: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito