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  1. Article ; Online: C

    Liu, Jin-Ling / Xu, Hong-Tao / Mu, Dan-Dan / Han, Xiao / Du, Ting-Ting / Wang, Yong-Li / Wei, Xiao-Hui / Chou, Gui-Xin

    Phytochemistry

    2023  Volume 213, Page(s) 113782

    Abstract: A phytochemical investigation of the dichloromethane soluble fraction of the ethanolic extract obtained from the roots of Marsdenia tenacissima led to the discovery of the sixteen undescribed pregnane ... ...

    Abstract A phytochemical investigation of the dichloromethane soluble fraction of the ethanolic extract obtained from the roots of Marsdenia tenacissima led to the discovery of the sixteen undescribed pregnane C
    MeSH term(s) Humans ; Marsdenia/chemistry ; Steroids/pharmacology ; Steroids/chemistry ; Pregnanes/chemistry ; Plant Extracts/chemistry ; Antineoplastic Agents
    Chemical Substances Steroids ; Pregnanes ; Plant Extracts ; Antineoplastic Agents
    Language English
    Publishing date 2023-07-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 208884-8
    ISSN 1873-3700 ; 0031-9422
    ISSN (online) 1873-3700
    ISSN 0031-9422
    DOI 10.1016/j.phytochem.2023.113782
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: C21 steroids from the roots of Marsdenia tenacissima

    Liu, Jin-Ling / Xu, Hong-Tao / Mu, Dan-Dan / Han, Xiao / Du, Ting-Ting / Wang, Yong-li / Wei, Xiao-Hui / Chou, Gui-Xin

    Phytochemistry. 20232023 Sept. 13, July 13, v. 213 p.113782-

    2023  

    Abstract: A phytochemical investigation of the dichloromethane soluble fraction of the ethanolic extract obtained from the roots of Marsdenia tenacissima led to the discovery of the sixteen undescribed pregnane C₂₁ steroids (1–16) and isolation of eleven known C₂₁ ...

    Abstract A phytochemical investigation of the dichloromethane soluble fraction of the ethanolic extract obtained from the roots of Marsdenia tenacissima led to the discovery of the sixteen undescribed pregnane C₂₁ steroids (1–16) and isolation of eleven known C₂₁ steroidal analogues (17–27). Their chemical structures were elucidated by one- and two-dimensional nuclear magnetic resonance spectroscopy and, high resolution–electrospray ionization mass spectrometry and their absolute configurations were determined using electronic circular dichroism or single-crystal X-ray diffraction. The in vitro anti-proliferative effects of 1–16 were evaluated against HepG2 (human hepatocellular cancer), A549 (lung cancer), and MCF-7 (human breast cancer) cell lines. Even though some of them showed moderate cytotoxic activities, marsectohexol derivative 12 exhibited significant cytotoxicity against A549 cells with an IC₅₀ value of 5.2 μM.
    Keywords Marsdenia tenacissima ; X-ray diffraction ; breast neoplasms ; circular dichroism spectroscopy ; cytotoxicity ; hepatoma ; humans ; lung neoplasms ; mass spectrometry ; methylene chloride ; nuclear magnetic resonance spectroscopy ; phytochemicals ; pregnanes ; Asclepiadaceae ; Pregnane ; C21 steroids ; Cytotoxic activity
    Language English
    Dates of publication 2023-0713
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 208884-8
    ISSN 1873-3700 ; 0031-9422
    ISSN (online) 1873-3700
    ISSN 0031-9422
    DOI 10.1016/j.phytochem.2023.113782
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  3. Article: [Separation and authentication of tilianin and quality standards of semen of Dracocephalum moldavia].

    Cheng, Xue-mei / Ma, Ting-yun / Su Ley-man / Ha-Lik / Mu, Dan-dan / Tiann Fang / Chou, Gui-Xin / Wang, Zheng-tao / Wang, Chang-hong

    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

    2015  Volume 40, Issue 10, Page(s) 1845–1849

    Abstract: Tilianin was separated and authenticated from the seeds of Dracocephalum moldavia, a Uygur medicine, by chromatographic technique and spectroscopic method. The purity of tilianin is more than 98% determined by HPLC area normalization method. Thin layer ... ...

    Abstract Tilianin was separated and authenticated from the seeds of Dracocephalum moldavia, a Uygur medicine, by chromatographic technique and spectroscopic method. The purity of tilianin is more than 98% determined by HPLC area normalization method. Thin layer chromatography (TLC) method was used to separate tilianin from D. moldavia by mixture of chloroform-methanol (5: 1) as a developing solvent on high performance silicagel precoated plate (SGF254) and using aluminium trichloride as a chromogenic agent for qualitative identification of D. moldavia. To establish a HPLC method for quantitative analysis of D. moldavia, tilianin was used as a Quantitative marker and separated on a C18 (4.6 mm x 250 mm, 5 μm) column with acetonitrile-01% formic acid (25: 75) as the mobile phase and detected at 330 nm. The calibration curve of tilianin displayed ideal linearity over the range of 0.617 2-123.44 μg x mL(-1) with a regression equation of Y = 33.773X - 0.824 8 (r = 1). The average recovery of tilianin was 101.0% with RSD of 3.7%. The RSD values of intra-day and inter-day precision were less than 2%. The content of tilianin in 4 batches of the authenticated semen of D. Moldavia was between 0.016 and 0.187 mg x g(-1). The qualitative and quantitative method established is suitable for the quality evaluation and assessment of semen of D. Moldavia.
    MeSH term(s) Chromatography, High Pressure Liquid ; Chromatography, Thin Layer ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/isolation & purification ; Drugs, Chinese Herbal/standards ; Flavonoids/chemistry ; Flavonoids/isolation & purification ; Flavonoids/standards ; Glycosides/chemistry ; Glycosides/isolation & purification ; Glycosides/standards ; Lamiaceae/chemistry ; Magnetic Resonance Spectroscopy ; Quality Control
    Chemical Substances Drugs, Chinese Herbal ; Flavonoids ; Glycosides ; tilianin
    Language Chinese
    Publishing date 2015-05
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1004649-5
    ISSN 1001-5302 ; 0254-0029
    ISSN 1001-5302 ; 0254-0029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3056: Show issues Location:
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  4. Article ; Online: Screening of Chinese herbal medicines for antityrosinase activity in a cell free system and B16 cells.

    Ye, Yan / Chou, Gui-Xin / Mu, Dan-Dan / Wang, Hui / Chu, Jian-Hong / Leung, Alexander Kai-Man / Fong, Wang-fun / Yu, Zhi-Ling

    Journal of ethnopharmacology

    2010  Volume 129, Issue 3, Page(s) 387–390

    Abstract: Aim of the study: Tyrosinase inhibitors are becoming increasingly important in controlling skin hyperpigmentation. We aimed to screen 50 extracts from traditional Chinese medicines (TCM) for tyrosinase activity-inhibiting agents.: Materials and ... ...

    Abstract Aim of the study: Tyrosinase inhibitors are becoming increasingly important in controlling skin hyperpigmentation. We aimed to screen 50 extracts from traditional Chinese medicines (TCM) for tyrosinase activity-inhibiting agents.
    Materials and methods: The 50 herbal extracts were prepared from 32 herbs and 18 TCM formulas, which are used as folk skin whiteners in China and have not been investigated for their skin-whitening mechanisms. Each herb and formula was extracted with 30% ethanol and water, respectively, and followed by column chromatography for isolating bioactive substances such as saponins, flavonoids and alkaloids for the antityrosinase activity study. Every extract was tested using the cell free mushroom tyrosinase inhibitory assay at 2 mg/ml for the single herb extracts and 1mg/ml for formula extracts. Extracts showing greater than 50% inhibition against mushroom tyrosinase activity were further examined by cellular tyrosinase assay in mouse B16 cells. The cytotoxicity in B16 cells was measured by methyl thiazolyl tetrazolium bromide (MTT) assay.
    Results: In the cell-free assay, 10 out of the 50 extracts demonstrated more than 50% inhibition against mushroom tyrosinase activity. These 10 extracts were further assessed by cellular tyrosinase assay, and 6 showed>50% inhibition with IC(50) values <1 mg/ml. The 6 extracts are from 3 herbs namely Ampelopsis japonica, Lindera aggregata, and Polygonatum odoratum, and 3 formulas namely Qian-wang-hong-bai-san, Qiong-yu-gao, and San-bai-tang. As compared with vitamin C, these 6 extracts showed similar or greater ratio of cell growth IC(50) to cellular tyrosinase IC(50). As compared with arbutin, extract from Ampelopsis japonica, Lindera aggregata, Qian-wang-hong-bai-san, or San-bai-tang had a similar, although extract from Polygonatum odoratum or Qiong-yu-gao had a greater, IC(50) value against murine tyrosinase activity.
    Conclusions: From the screening assays we identified three Chinese medicinal herbs and three TCM formulas that have appreciable antityrosinase activity. Further studies are warranted to develop them as skin-whitening agents with convenient dosage forms or to identify active constituents from the extracts as useful leads for the development of skin whiteners.
    MeSH term(s) Agaricales/enzymology ; Animals ; Cell Line, Tumor ; Cell Survival/drug effects ; Drugs, Chinese Herbal/pharmacology ; Enzyme Inhibitors/pharmacology ; Medicine, Chinese Traditional ; Mice ; Monophenol Monooxygenase/antagonists & inhibitors ; Skin Pigmentation/drug effects ; Spectrophotometry
    Chemical Substances Drugs, Chinese Herbal ; Enzyme Inhibitors ; Monophenol Monooxygenase (EC 1.14.18.1)
    Language English
    Publishing date 2010-06-16
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2010.04.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Screening of Chinese herbal medicines for antityrosinase activity in a cell free system and B16 cells

    Ye, Yan / Chou, Gui-Xin / Mu, Dan-Dan / Wang, Hui / Chu, Jian-Hong / Leung, Alexander Kai-Man / Fong, Wang-fun / Yu, Zhi-Ling

    Journal of ethnopharmacology. 2010 June 16, v. 129, no. 3

    2010  

    Abstract: Aim of the study: Tyrosinase inhibitors are becoming increasingly important in controlling skin hyperpigmentation. We aimed to screen 50 extracts from traditional Chinese medicines (TCM) for tyrosinase activity-inhibiting agents. Materials and methods: ... ...

    Abstract Aim of the study: Tyrosinase inhibitors are becoming increasingly important in controlling skin hyperpigmentation. We aimed to screen 50 extracts from traditional Chinese medicines (TCM) for tyrosinase activity-inhibiting agents. Materials and methods: The 50 herbal extracts were prepared from 32 herbs and 18 TCM formulas, which are used as folk skin whiteners in China and have not been investigated for their skin-whitening mechanisms. Each herb and formula was extracted with 30% ethanol and water, respectively, and followed by column chromatography for isolating bioactive substances such as saponins, flavonoids and alkaloids for the antityrosinase activity study. Every extract was tested using the cell free mushroom tyrosinase inhibitory assay at 2mg/ml for the single herb extracts and 1mg/ml for formula extracts. Extracts showing greater than 50% inhibition against mushroom tyrosinase activity were further examined by cellular tyrosinase assay in mouse B16 cells. The cytotoxicity in B16 cells was measured by methyl thiazolyl tetrazolium bromide (MTT) assay. Results: In the cell-free assay, 10 out of the 50 extracts demonstrated more than 50% inhibition against mushroom tyrosinase activity. These 10 extracts were further assessed by cellular tyrosinase assay, and 6 showed>50% inhibition with IC₅₀ values <1mg/ml. The 6 extracts are from 3 herbs namely Ampelopsis japonica, Lindera aggregata, and Polygonatum odoratum, and 3 formulas namely Qian-wang-hong-bai-san, Qiong-yu-gao, and San-bai-tang. As compared with vitamin C, these 6 extracts showed similar or greater ratio of cell growth IC₅₀ to cellular tyrosinase IC₅₀. As compared with arbutin, extract from Ampelopsis japonica, Lindera aggregata, Qian-wang-hong-bai-san, or San-bai-tang had a similar, although extract from Polygonatum odoratum or Qiong-yu-gao had a greater, IC₅₀ value against murine tyrosinase activity. Conclusions: From the screening assays we identified three Chinese medicinal herbs and three TCM formulas that have appreciable antityrosinase activity. Further studies are warranted to develop them as skin-whitening agents with convenient dosage forms or to identify active constituents from the extracts as useful leads for the development of skin whiteners.
    Keywords herbal medicines ; Oriental traditional medicine ; medicinal plants ; screening ; oxidoreductases ; enzyme inhibition ; enzyme inhibitors ; skin diseases ; pigmentation ; plant extracts ; bioactive properties ; in vitro studies ; dosage ; mice ; animal models ; cytotoxicity ; inhibitory concentration 50 ; cell growth
    Language English
    Dates of publication 2010-0616
    Size p. 387-390.
    Publishing place Amsterdam; New York: Elsevier
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2010.04.009
    Database NAL-Catalogue (AGRICOLA)

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