LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Mudrak, Nathan J"
  2. AU="Fagundes, Addller Oliveira"
  3. AU="Qi, Xiaoli" AU="Qi, Xiaoli"
  4. AU="Durymanov, Mikhail"
  5. AU=Yin Wenzhe
  6. AU=Vlachos Ioannis S.
  7. AU=Grundy Scott M
  8. AU="Koumoutsea, Evangelia Vlachodimitropoulou"
  9. AU="Almen, Aimee"
  10. AU="Howard, Dianna S."
  11. AU="Elizete Rizzo"
  12. AU="El Sayegh, Suzanne"
  13. AU="Vaittinen, Tiina"
  14. AU="Khir, Amir S"
  15. AU=Patterson Andrew D
  16. AU="Kim, Joyce Mary"
  17. AU="Saribay, S Adil"
  18. AU="Couderc, M."
  19. AU="Macerlane de Lira Silva"
  20. AU=Neal Michael S
  21. AU="Nakai, Kozo"
  22. AU="Debatin, Jörg F."
  23. AU="Plant, Laura"
  24. AU="Manuel Tisminetzky"
  25. AU="Monaco, Carlo"
  26. AU="Srivastava, Rupesh"
  27. AU="Nathan, Jaimie D"
  28. AU="Schnegelberger, Regina D"
  29. AU=Doshi Paresh
  30. AU="Cecilia Hognon"
  31. AU="Mason, Jeremy K."
  32. AU=Hasumi Hisashi
  33. AU="Swati Sethi"
  34. AU="Martin G. Myers, Jr."
  35. AU="Marcus-Sekura, Carol"
  36. AU="Petagine, Lucy"
  37. AU="Jessa R. Alexander"
  38. AU=Rauner Martina
  39. AU="Richlen, Mindy L"
  40. AU="Merghani, Nada M"
  41. AU=Splitt M P
  42. AU="Zlatanović, Gordana"

Suchergebnis

Treffer 1 - 5 von insgesamt 5

Suchoptionen

  1. Artikel ; Online: Calibrated brightfield-based imaging for measuring intracellular protein concentration.

    Mudrak, Nathan J / Rana, Priyanka S / Model, Michael A

    Cytometry. Part A : the journal of the International Society for Analytical Cytology

    2017  Band 93, Heft 3, Seite(n) 297–304

    Abstract: Intracellular protein concentration is an essential cell characteristic, which manifests itself through the refractive index. The latter can be measured from two or more mutually defocused brightfield images analyzed using the TIE (transport-of-intensity ...

    Abstract Intracellular protein concentration is an essential cell characteristic, which manifests itself through the refractive index. The latter can be measured from two or more mutually defocused brightfield images analyzed using the TIE (transport-of-intensity equation). In practice, however, TIE does not always achieve quantitatively accurate results on biological cells. Therefore, we have developed a calibration procedure that involves successive imaging of cells in solutions containing different amounts of added protein. This allows one to directly relate the output of TIE (T) to intracellular protein concentration C (g/L). The resultant relationship has a simple form: C ≈ 1.0(T/V), where V is the cell volume (μm
    Mesh-Begriff(e) Animals ; Cell Line, Tumor ; Cell Size ; Dogs ; HeLa Cells ; Humans ; Image Processing, Computer-Assisted/methods ; Madin Darby Canine Kidney Cells ; Osmolar Concentration ; Proteins/analysis
    Chemische Substanzen Proteins
    Sprache Englisch
    Erscheinungsdatum 2017-05-31
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2099868-5
    ISSN 1552-4930 ; 0196-4763 ; 1552-4922
    ISSN (online) 1552-4930
    ISSN 0196-4763 ; 1552-4922
    DOI 10.1002/cyto.a.23145
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1688: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 68: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel ; Online: Staurosporine-induced apoptotic water loss is cell- and attachment-specific.

    Model, Michael A / Mudrak, Nathan J / Rana, Priyanka S / Clements, Robert J

    Apoptosis : an international journal on programmed cell death

    2018  Band 23, Heft 7-8, Seite(n) 449–455

    Abstract: Apoptotic volume decrease (AVD) is a characteristic cell shrinkage observed during apoptosis. There are at least two known processes that may result in the AVD: exit of intracellular water and splitting of cells into smaller fragments. Although AVD has ... ...

    Abstract Apoptotic volume decrease (AVD) is a characteristic cell shrinkage observed during apoptosis. There are at least two known processes that may result in the AVD: exit of intracellular water and splitting of cells into smaller fragments. Although AVD has traditionally been attributed to water loss, direct evidence for that is often lacking. In this study, we quantified intracellular water in staurosporine-treated cells using a previously described optical microscopic technique that combines volume measurements with quantitative phase analysis. Water loss was observed in detached HeLa and in adherent MDCK but not in adherent HeLa cells. At the same time, adherent HeLa and adherent MDCK cells exhibited visually similar apoptotic morphology, including fragmentation and activation of caspase-3. Morphological changes and caspase activation were prevented by chloride channel blockers DIDS and NPPB in both adherent and suspended HeLa cells, while potassium channel blocker TEA was ineffective. We conclude that staurosporine-induced dehydration is not a universal cell response but depends on the cell type and substrate attachment and can only be judged by direct water measurements. The effects of potassium or chloride channel blockers do not always correlate with the AVD.
    Mesh-Begriff(e) Animals ; Apoptosis/drug effects ; Cell Size/drug effects ; Dogs ; Enzyme Inhibitors/pharmacology ; HeLa Cells ; Humans ; Madin Darby Canine Kidney Cells ; Potassium/metabolism ; Potassium Channel Blockers/pharmacology ; Staurosporine/pharmacology ; Water/metabolism
    Chemische Substanzen Enzyme Inhibitors ; Potassium Channel Blockers ; Water (059QF0KO0R) ; Staurosporine (H88EPA0A3N) ; Potassium (RWP5GA015D)
    Sprache Englisch
    Erscheinungsdatum 2018-06-29
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1452360-7
    ISSN 1573-675X ; 1360-8185
    ISSN (online) 1573-675X
    ISSN 1360-8185
    DOI 10.1007/s10495-018-1471-x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 5178: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralization.

    McConnell, Scott A / Sachithanandham, Jaiprasath / Mudrak, Nathan J / Zhu, Xianming / Farhang, Parsa Alba / Cordero, Radames J B / Wear, Maggie P / Shapiro, Janna R / Park, Han-Sol / Klein, Sabra L / Tobian, Aaron A R / Bloch, Evan M / Sullivan, David J / Pekosz, Andrew / Casadevall, Arturo

    Cell chemical biology

    2023  Band 30, Heft 7, Seite(n) 726–738.e4

    Abstract: Understanding the mechanisms of antibody-mediated neutralization of SARS-CoV-2 is critical in combating the COVID-19 pandemic. Based on previous reports of antibody catalysis, we investigated the proteolysis of spike (S) by antibodies in COVID-19 ... ...

    Abstract Understanding the mechanisms of antibody-mediated neutralization of SARS-CoV-2 is critical in combating the COVID-19 pandemic. Based on previous reports of antibody catalysis, we investigated the proteolysis of spike (S) by antibodies in COVID-19 convalescent plasma (CCP) and its contribution to viral neutralization. Quenched fluorescent peptides were designed based on S epitopes to sensitively detect antibody-mediated proteolysis. We observed epitope cleavage by CCP from different donors which persisted when plasma was heat-treated or when IgG was isolated from plasma. Further, purified CCP antibodies proteolyzed recombinant S domains, as well as authentic viral S. Cleavage of S variants suggests CCP antibody-mediated proteolysis is a durable phenomenon despite antigenic drift. We differentiated viral neutralization occurring via direct interference with receptor binding from that occurring by antibody-mediated proteolysis, demonstrating that antibody catalysis enhanced neutralization. These results suggest that antibody-catalyzed damage of S is an immunologically relevant function of neutralizing antibodies against SARS-CoV-2.
    Mesh-Begriff(e) Humans ; Proteolysis ; SARS-CoV-2 ; Pandemics ; COVID-19/therapy ; COVID-19 Serotherapy ; Spike Glycoprotein, Coronavirus ; Peptide Hydrolases ; Antibodies, Neutralizing ; Epitopes ; Antibodies, Viral
    Chemische Substanzen spike protein, SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Peptide Hydrolases (EC 3.4.-) ; Antibodies, Neutralizing ; Epitopes ; Antibodies, Viral
    Sprache Englisch
    Erscheinungsdatum 2023-06-23
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 2451-9448
    ISSN (online) 2451-9448
    DOI 10.1016/j.chembiol.2023.05.011
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  4. Artikel ; Online: Melanization of

    Smith, Daniel F Q / Mudrak, Nathan J / Zamith-Miranda, Daniel / Honorato, Leandro / Nimrichter, Leonardo / Chrissian, Christine / Smith, Barbara / Gerfen, Gary / Stark, Ruth E / Nosanchuk, Joshua D / Casadevall, Arturo

    Journal of fungi (Basel, Switzerland)

    2022  Band 8, Heft 10

    Abstract: ... Candida ... ...

    Abstract Candida auris
    Sprache Englisch
    Erscheinungsdatum 2022-10-11
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8101068
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel ; Online: Phase separation in necrotic cells.

    Rana, Priyanka S / Mudrak, Nathan J / Lopez, Roxlee / Lynn, Matthew / Kershner, Leah / Model, Michael A

    Biochemical and biophysical research communications

    2017  Band 492, Heft 3, Seite(n) 300–303

    Abstract: Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: ... ...

    Abstract Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2. How can osmotic equilibrium be maintained between the bleb and CB? Our photobleaching and ultracentrifugation experiments indicate extensive protein aggregation. We hypothesize that protein aggregation within the CB shifts the chemical equilibrium and draws proteins out of the bleb; at the same time, aggregation reduces the effective molar concentration of protein in the CB, so that osmotic equilibrium between high-protein CB and low-protein necrotic blebs becomes possible.
    Mesh-Begriff(e) Cell Body/chemistry ; Cell Body/metabolism ; Cell Fractionation ; Cell Membrane/metabolism ; HeLa Cells ; Humans ; Necrosis/metabolism ; Protein Aggregates ; Proteins/analysis ; Proteins/metabolism
    Chemische Substanzen Protein Aggregates ; Proteins
    Sprache Englisch
    Erscheinungsdatum 2017-10-21
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2017.08.123
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 116: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang