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  1. Article ; Online: ANTIBIOTIC SUSCEPTIBILITY/RESISTANCE AMONG SALMONELLA SPECIES ISOLATED IN NON-HOSPITALISED PATIENTS IN THE CANTON OF SARAJEVO

    Mufida Aljicevic / Armin Cikotic / Sabaheta Bektas / Sabina Mahmutovic Vranic / Velma Rebic / Amila Abduzaimovic / Adem Cemerlic

    Journal of IMAB, Vol 25, Iss 2, Pp 2532-

    2019  Volume 2536

    Abstract: Background: Salmonellosis, a disease which occurs following the consumption of food contaminated with salmonella, is most frequently present in young children and older immunocompromised individuals. This condition must be treated with antibiotics. The ... ...

    Abstract Background: Salmonellosis, a disease which occurs following the consumption of food contaminated with salmonella, is most frequently present in young children and older immunocompromised individuals. This condition must be treated with antibiotics. The increased degree of morbidity comes in relation to the increased resistance of Sallmonellae species to antibiotics. Resistance has considerably been increased in approximately the last twenty years, thanks mostly to the establishment of diagnoses ex juvantibus, the prescription of therapies without antimicrobial susceptibility/resistance testing, as well as the massive, unjustified usage of antibiotics. Purpose: The goal of this study was to examine the representation of antibiotic susceptibility/resistance of Salmonella species isolated in non-hospitalised patients. Materials and methods: In a microbiological laboratory in the Institute for Public Health Canton of Sarajevo (Bosnia and Herzegovina), analysed were samples of stool and urine of non-hospitalised patients with our without developed symptoms of salmonellosis in the period from one year. Results: Of the total 69 patients infected with salmonella, in 65 (94.2%) S. enteritidis group D was isolated, in 3 (4.3%) S. species group B, and in 1 (1.4%) patient there was a S. enteritidis group D and a S. species group C. Isolated strains were resistant to Ampicillin in 63 (91.3%) patients, while to Ciprofloxacin and Bactrim there was no established resistance. Conclusions: The most commonly isolated bacterial strain was S. enteritidis group D. Isolated strains show different susceptibility/resistance to tested antibiotics, and thus the completion of an antimicrobial susceptibility/resistance test is necessary before adding an antibiotic to the patient’s treatment.
    Keywords Salmonella species ; salmonellosis ; antimicrobial susceptibility test ; Dentistry ; RK1-715 ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2019-05-01T00:00:00Z
    Publisher Peytchinski Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Rat bone marrow stem cells isolation and culture as a bone formative experimental system

    Amer Smajilagić / Mufida Aljičević / Amira Redžić / Selma Filipović / Alena C. Lagumdžija

    Bosnian Journal of Basic Medical Sciences, Vol 13, Iss

    2013  Volume 1

    Abstract: Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe ... ...

    Abstract Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bosnia and Herzegovina, of the isolation and differentiation rat bone marrow stromal cells in to the osteoblast cells lineages. Rat bone marrow stromal cells were isolated by method described by Maniatopulos using their plastic adherence capatibility. The cells obtained by plastic adherence were cultured and serially passaged in the osteoinductive medium to differentiate into the osteocytes. Bone marrow samples from rats long bones used for isolation of stromal cells (BMSCs). Under determinate culture conditions BMSCs were differentiated in osteogenic cell lines detected by Alizarin red staining three weeks after isolation. BMSCs as autologue cells model showed high osteogenetic potential and calcification capatibility in vitro. In future should be used as alternative method for bone transplantation in Regenerative Medicine.
    Keywords Adult stem cells ; bone marrow ; bone marrow stem cells ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2013-02-01T00:00:00Z
    Publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Demonstration of different endocervical staining methods and their usefulness in the diagnosis of the chlamydial infection in exfoliated cells advantages and disadvantages

    Sabina Mahmutović / Edina Bešlagić / Sadeta Hamzić / Mufida Aljicević

    Bosnian Journal of Basic Medical Sciences, Vol 4, Iss

    2004  Volume 1

    Abstract: Microscopic demonstration of chlamydial inclusions within cells offered the first laboratory procedure supporting the clinical diagnosis of chlamydial infection. Our aim is to evaluate the usefulness of different endocervical staining methods in ... ...

    Abstract Microscopic demonstration of chlamydial inclusions within cells offered the first laboratory procedure supporting the clinical diagnosis of chlamydial infection. Our aim is to evaluate the usefulness of different endocervical staining methods in diagnosis of Chlamydia trachomatis (CT) infection within exfoliated cells of the endocervix. The cytological test for the detection of chlamydial inclusions in genital tract infection, though not as sensitive and specific as isolation in the cell culture monolayers, is still of the diagnostic value. The present study discusses the collection of clinical smears for microscopic examination, their preparation; fixation and staining of slides by a variety of staining methods that have been used to detect Chlamydia in clinical smears and biopsies. Most of these methods such as Giemsa stain, Papanicolaou, iodine, and immunofluorescence (IF) using monoclonal antibodies, are based on the combination of dyes designed to obtain optimum differentiation of the various structures. The utilization of different endocervical smear stains together with the clinical information can be used to identify women at high risk for CT infection.
    Keywords Endocervical Stain ; Giemsa ; Papanicolaou ; Iodine ; Immunofluorescence ; Chlamydia Trachomatis ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2004-02-01T00:00:00Z
    Publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Listeria monocytogenes as the possible cause of the spontanous abortion in female of the fertile age

    Mufida Aljičević / Edina Bešlagić / Šukrija Zvizdić / Sadeta Hamzić / Sabina Mahmutović

    Bosnian Journal of Basic Medical Sciences, Vol 5, Iss

    2005  Volume 4

    Abstract: Listeria monocytogenes is a Gram-positive, weakly pathogenic bacterium able to grow also at the temperature of 40 degrees C. A man most often gets affected by consuming contaminated food and water. Animals can carry bacteria although they have not to ... ...

    Abstract Listeria monocytogenes is a Gram-positive, weakly pathogenic bacterium able to grow also at the temperature of 40 degrees C. A man most often gets affected by consuming contaminated food and water. Animals can carry bacteria although they have not to appear ill, then their meat and milk products are the source of infection of human being. The disease most often attacks with the weak immune system, newborns and pregnant women. That what is significant, listeria penetrates through the placenta and can lead to the fatal infection, which is characteristic by disseminated granulomatosis lesions of a newborn and micro abscess on the placenta. In a newborn can provoke the sepsis with the mortality of 50%. By the examination are encircled two groups of the reproductive age-totally 60. From these 30 had one or more spontaneous abortion, and 30 had no spontaneous abortion. By the serologic reaction the agglutination is discovered the presence of the antibodies in serum of the. The positive serologic answer was found in the first group in 18 (60%) and in that another group in 8 participants (26, 70%).
    Keywords serologic response ; Listeria monocytogenes ; Medicine (General) ; R5-920
    Subject code 630
    Language English
    Publishing date 2005-11-01T00:00:00Z
    Publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Serotesting of Human Brucellosis on Wider Area of Bosnia and Herzegovina

    Sadeta Hamzić / Edina Bešagić / Šukrija Zvizdić / Mufida Aljičević / Omer Bešlagić / Sandra Puvačić

    Bosnian Journal of Basic Medical Sciences, Vol 5, Iss

    2005  Volume 3

    Abstract: The study involved 286 individuals from different regions of Bosnia and Herzegovina, whose sera were tested in the Laboratory for specific diagnosis of human brucellosis in Microbiology Department of Medical Faculty of University in Sarajevo, during the ... ...

    Abstract The study involved 286 individuals from different regions of Bosnia and Herzegovina, whose sera were tested in the Laboratory for specific diagnosis of human brucellosis in Microbiology Department of Medical Faculty of University in Sarajevo, during the period from 2000. to 2003. Sera were tested using Brucelloslide Test, qualitative agglutination test Rose Bengal. Using the agglutination test, we serologically confirmed a diagnosis of human brucellosis in 59 (20.62%) seropositive individuals, whereof 38 (64.40%) men and 21 (35.60%) women. Individuals with human brucellosis were the most present in the age group of 31-40 (22.03%) and 41-50 (22.03%). One serologically confirmed death case was registered. The most seropositive individuals were from Zenica-Doboj Canton (32.20%), Sarajevo Canton (28.82%), Herzegovina-Neretva Canton (23.73%), Central Bosnia Canton (13.55%) and Una-Sana Canton (1.70%). During our four-year study, it was serologically confirmed that human brucellosis is present in Bosnia and Herzegovina and, through seropositive testing, we revealed the level of general exposition to Brucella spp. on wider area of Bosnia and Herzegovina.
    Keywords Brucella ; human brucellosis ; serodiagnosis ; Bosnia and Herzegovina ; Medicine (General) ; R5-920
    Subject code 360
    Language English
    Publishing date 2005-08-01T00:00:00Z
    Publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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