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  1. Article ; Online: In silico view of MTA1 biochemical signatures in breast malignancy for improvement in immunosurveillance

    Zafar Abbas Shah / Humaira Yasmin / Faisal Nouroz / Muhammad Delwar Hussain / Mohsin Kazi

    Journal of King Saud University: Science, Vol 34, Iss 3, Pp 101843- (2022)

    2022  

    Abstract: Metastasis Associated 1 (MTA1) chromatin modifier oncoprotein played a crucial role in both normal and genotoxic stress situations for genome maintenance. To investigates MTA1 regulatory pattern with drug resistance abilities in breast primary carcinoma ... ...

    Abstract Metastasis Associated 1 (MTA1) chromatin modifier oncoprotein played a crucial role in both normal and genotoxic stress situations for genome maintenance. To investigates MTA1 regulatory pattern with drug resistance abilities in breast primary carcinoma to advanced invasive stages for identification of cancer adapting defense system. We design rationale in silico pipeline from data retrieval to analysis i.e. gene enrichment analysis performed by GeneCards Version 5.1: THE HUMAN GENE DATABASE (www.genecards.org), UALCAN database (www.ualcan.path.uab.edu) for analyzing MTA1 gene expression and promoter methylation in both breasts normal and cancerous tissue samples, cBioPortal for Cancer Genomics (www.cbioportal.org) database for MTA1 mutation analysis and finally analyzed MTA1 functional association with anticancer drugs in breast malignancy via online CCLE GDSC toolkit (www.public.tableau.com/CCLE_GDSC_Correlations). Our results revealed MTA1 overexpression aggressive behavior in stage II, stage III, TNBC-LAR, TNBC-M, TNBC-UNS, IDC, ILC, and post-menopause events of breast malignancy. MTA1 upregulation strongly promotes primary tumor transformation into invasive metastatic carcinoma by hijacking the host lymphatic system and cytokine signaling in both ductal and glandular breast cancers. MTA1 upregulation in the African-American population invites to design de novo model of cancer cell homeostasis under a reduced supply of vegetable nutrients, local-foreign stress, and replicative capacity for metastasis. MTA1 showed a hypomethylation profile that reflects regulatory strength under stress-mediated situations for higher events of transcription. In drug resistance analysis MTA1 has strong resistance towards 15 anti-cancer drugs that confirmed its previously reported behavior of genotoxic stress adaptation for metastasis. Our in silico evidence invites us to design a comprehensive strategy against MTA1 mediated stress managing proteome. In the future there is an urgent need to explore MTA1 shared stress coped ...
    Keywords MTA1 ; Breast invasive carcinoma ; Genotoxic stress ; Proliferation ; Differentiation ; Apoptotic signaling ; Science (General) ; Q1-390
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: The Development and Optimization of Lipid-Based Self-Nanoemulsifying Drug Delivery Systems for the Intravenous Delivery of Propofol

    Mohsin Kazi / Athba Alqahtani / Majed Alharbi / Ajaz Ahmad / Muhammad Delwar Hussain / Hani Alothaid / Mohammed S. Aldughaim

    Molecules, Vol 28, Iss 1492, p

    2023  Volume 1492

    Abstract: Purpose: Propofol is a relatively short-acting potent anesthetic lipophilic drug used during short surgical procedures. Despite the success of propofol intravenous emulsions, drawbacks to such formulations include inherent emulsion instability, the lack ... ...

    Abstract Purpose: Propofol is a relatively short-acting potent anesthetic lipophilic drug used during short surgical procedures. Despite the success of propofol intravenous emulsions, drawbacks to such formulations include inherent emulsion instability, the lack of a safe vehicle to prevent sepsis, and concern regarding hyperlipidemia-related side effects. The aim of the current investigation was to develop a novel, lipid-based self-nanoemulsifying drug delivery system (SNEDDS) for propofol with improved stability and anesthetic activity for human use. Methods: A series of SNEDDS formulations were developed using naturally obtained medium-chain/long-chain mono-, di-, and triglycerides, glyceryl monocaprylate, and water-soluble cosolvents with hydrogenated castor oil constructing ternary phase diagrams for propofol. The developed SNEDDS formulations were characterized using visual observation, particle size analysis, zeta potential, transmission electron microscopy, equilibrium solubility, in vitro dynamic dispersion and stability, and in vivo sleeping disorder studies in rats. The in vivo bioavailability of the SNEDDSs in rats was also studied to compare the representative formulations with the marketed product Diprivan ® . Results: Medium-chain triglycerides (M810) with mono-diglycerides (CMCM) as an oil blend and hydrogenated castor oil (KHS15) as a surfactant were selected as key ingredients in ternary phase diagram studies. The nanoemulsifying regions were identified from the studies and a number of SNEDDSs were formulated. Results from the characterization studies demonstrated the formation of efficient nanosized particles (28–45 nm globule size, 0.10–0.20 PDI) in the optimized SNEDDS with a drug loading of 50 mg/g, which is almost 500-fold higher than free propofol. TEM analysis showed the formation of spherical and homogeneous nanoparticles of less than 50 nm. The dissolution rate of the representative SNEDDS was faster than raw propofol and able to maintain 99% propofol in aqueous solution for around 24 h. The ...
    Keywords self-nanoemulsifying drug delivery systems ; propofol ; pharmacokinetics ; sleeping disorder ; intravenous delivery ; Organic chemistry ; QD241-441
    Subject code 660
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: A fraction of Pueraria tuberosa extract, rich in antioxidant compounds, alleviates ovariectomized-induced osteoporosis in rats and inhibits growth of breast and ovarian cancer cells.

    Swaha Satpathy / Arjun Patra / Muhammad Delwar Hussain / Mohsin Kazi / Mohammed S Aldughaim / Bharti Ahirwar

    PLoS ONE, Vol 16, Iss 1, p e

    2021  Volume 0240068

    Abstract: Pueraria tuberosa (Roxb. ex Willd.) DC., known as Indian Kudzu belongs to family Fabaceae and it is solicited as "Rasayana" drugs in Ayurveda. In the present study, we analyzed the efficacy of an ethyl acetate fraction from the tuber extract of Pueraria ... ...

    Abstract Pueraria tuberosa (Roxb. ex Willd.) DC., known as Indian Kudzu belongs to family Fabaceae and it is solicited as "Rasayana" drugs in Ayurveda. In the present study, we analyzed the efficacy of an ethyl acetate fraction from the tuber extract of Pueraria tuberosa (fraction rich in antioxidant compounds, FRAC) against menopausal osteoporosis, and breast and ovarian cancer cells. The FRAC from Pueraria tuberosa was characterized for its phenolic composition (total phenolic and flavonoid amount). Antioxidant property (in vitro assays) of the FRAC was also carried out followed by the analysis of the FRAC for its antiosteoporotic and anticancer potentials. The antiosteoporotic activity of FRAC was investigated in ovariectomy-induced osteoporosis in rats. The cytotoxicity effect was determined in breast and ovarian cancer cells. Gas chromatography/mass spectrometry (GC/MS) analysis of the FRAC was performed to determine its various phytoconstituents. Docking analysis was performed to verify the interaction of bioactive molecules with estrogen receptors (ERs). The FRAC significantly improved various biomechanical and biochemical parameters in a dose-dependent manner in the ovariectomized rats. FRAC also controlled the increased body weight and decreased uterus weight following ovariectomy in rats. Histopathology of the femur demonstrated the restoration of typical bone structure and trabecular width in ovariectomized animals after treatment with FRAC and raloxifene. The FRAC also exhibited in vitro cytotoxicity in the breast (MCF-7 and MDA-MB-231) and ovarian (SKOV-3) cancer cells. Furthermore, genistein and daidzein exhibited a high affinity towards both estrogen receptors (α and β) in the docking study revealing the probable mechanism of the antiosteoporotic activity. GC/MS analysis confirmed the presence of other bioactive molecules such as stigmasterol, β-sitosterol, and stigmasta-3,5-dien-7-one. The FRAC from Pueraria tuberosa has potential for treatment of menopausal osteoporosis. Also, the FRAC possesses ...
    Keywords Medicine ; R ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: In vitro cytotoxic potential of cow dung and expired tomato sauces-derived carbon nanodots against A-375 human melanoma cell line

    Gaurav Gopal Naik / Reena Madavi / Tarun Minocha / Debadatta Mohapatra / Ravi Pratap / Singh Shreya / Pradeep Kumar Patel / Sanjeev Kumar Yadav / Avanish Parmar / Arjun Patra / Nishant Sudhir Jain / Swaha Satpathy / Mohsin Kazi / Muhammad Delwar Hussain / Alakh N Sahu

    Arabian Journal of Chemistry, Vol 17, Iss 2, Pp 105576- (2024)

    2024  

    Abstract: Converting biowaste into a functional product is put to the test by the growing amount of biowaste in the world and the environmental problems it causes. In this research study, we synthesized, characterized, and evaluated bluish-green luminescent carbon ...

    Abstract Converting biowaste into a functional product is put to the test by the growing amount of biowaste in the world and the environmental problems it causes. In this research study, we synthesized, characterized, and evaluated bluish-green luminescent carbon nanodots (CNDs) from cow dung and expired tomato sauces via a hydrothermal synthesis method at 160 °C for 8 h. The carbon nanodots were fabricated without additional passivating agents and exhibited good physicochemical and optical properties. The intrinsic properties of carbon nanodots were characterized using various spectral techniques. First, we evaluated the cytotoxic potential of carbon nanodots against A-375 human melanoma cell lines. This study revealed that carbon nanodots exhibited potent cytotoxicity and significantly inhibited the proliferation of A-375 cells in a dose-dependent manner. Next, we demonstrated these carbon nanodot's free radical scavenging potential by employing 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The bluish-green fluorescent carbon nanodots fabricated using a green synthesis approach have broad potential for biological applications.
    Keywords Green chemistry ; Biomass ; Carbon nanodots ; Nanoparticles ; Melanoma ; Fluorescence ; Chemistry ; QD1-999
    Language English
    Publishing date 2024-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Analgesic Effects of Pre-induction Low-dose Ketamine on Post-tonsillectomy Patients

    Muhammad Delwar Hussain / Md Torab Mallick / Mohammad Kamal Hossain

    Journal of Enam Medical College, Vol 8, Iss 2, Pp 74-

    2018  Volume 79

    Abstract: Background: Post-operative pain management aims to decrease pain intensity with patient comfort and to improve post-operative outcome. Multimodal analgesia is currently recommended for effective post-operative pain control with lower total doses of ... ...

    Abstract Background: Post-operative pain management aims to decrease pain intensity with patient comfort and to improve post-operative outcome. Multimodal analgesia is currently recommended for effective post-operative pain control with lower total doses of analgesics and fewer side effects. Objective: To compare the analgesic effect of pre-induction low-dose ketamine against conventional general anaesthesia. Materials and Methods: This prospective comparative study was conducted over a period of 12 months at Combined Military Hospital, Dhaka. Two hundred and forty patients of either sex requiring tonsillectomy were divided into two groups: conventional general anaesthesia (control group) and general anaesthesia with low-dose ketamine (ketamine group). Non-invasive blood pressure, heart rate and SpO2 were recorded at regular intervals throughout the anaesthetic period. Post-operative analgesia was provided for both the groups using pethidine intramuscularly. Time to complete operation, pain intensity, time to request for first analgesia were noted and total opioid consumption and complications, if any occurred in 24 hours post-operatively, were also recorded and addressed accordingly. Results: The demographic data and mean operation time were similar in both the groups (p>0.05).Time to request for first analgesia was longer in lowdose ketamine group (mean ± SD 5.36±3.21 hours) than in control group (mean ± SD 2.49±1.53 hours) (p<0.05). Total dosage of pethidine consumption over 24 hours period was less in ketamine group with satisfactory pain relief (mean ± SD 98.73±2.60 mg) than in control group (mean ± SD 142.52±3.48 mg) (p<0.05). Post-operative complications were also less in ketamine group than control group (p<0.05). Conclusion: The result of this study suggests that pre-induction low-dose ketamine has pre-emptive analgesic effect and reduces overall post-operative opioid requirements.
    Keywords Medicine ; R
    Subject code 630
    Language English
    Publishing date 2018-05-01T00:00:00Z
    Publisher Enam Medical College, Dhaka
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Chemical characterization and evaluation of the nephroprotective potential of Parrotiopsis jacquemontiana (Decne) Rehder and Periploca hydaspidis Falc crude extract in CCl4-induced Male Sprague-Dawley Rats

    Saima Ali / Muhammad Rashid Khan / Javed Iqbal / Sayed Afzal Shah / Banzeer Ahsan Abbasi / Tabassum Yaseen / Riffat Batool / Iftikhar Ali / Muhammad Delwar Hussain / Mohsin Kazi

    Saudi Journal of Biological Sciences, Vol 29, Iss 2, Pp 702-

    2022  Volume 712

    Abstract: Biochemical, antioxidant, serum, and urine profiles together with physical examination can deliver important information regarding animal health status, and are vital in the diagnosis and treatment of patients. CCl4, a potent nephrotoxin, was used for ... ...

    Abstract Biochemical, antioxidant, serum, and urine profiles together with physical examination can deliver important information regarding animal health status, and are vital in the diagnosis and treatment of patients. CCl4, a potent nephrotoxin, was used for causing toxicity in rat kidneys. The present study aimed at exploring the nephroprotective potential of P. jacquemontiana leaves methanol extract (PJM) and P. hydaspidis whole-plant methanol extract (PHM) on kidney cells of male rats after oxidative stress and DNA damage was instigated by CCl4. Various parameters including enzymatic levels, serum profiles, urine profiles, genotoxicity, and histological studies were conducted. In renal samples of rats treated with CCl4, the antioxidant enzymes (POD, SOD, CAT), PH level, protein level, and glutathione contents were significantly (p < 0.05) declined whereas renal biochemicals (H2O2, TBARS, and nitrite), specific gravity, level of urea, urobilinogen, serum BUN and creatinine were markedly (p < 0.05) increased relative to control group. Co-administration of PJM and PHM with CCl4 displayed protective ability against CCl4 intoxication by restoring activities of antioxidant enzymes, urine profile, biochemical parameters, and serum profile in rats. CCl4 also induced prominent DNA damages and glomerular atrophy with abnormal appearance of glomerulus and Bowman’s capsule. These damages results in impaired corticular sections, edema in Bowman’s capsule, accumulation of necrotic cells, dilation of convoluted tubules, and narrowing of space between Bowman’s capsule, which were successfully ameliorated after co-administration of PJM and PHM fractions in a dose-dependent manner (200 and 400 mg/kg b.w.). The results obtained suggest the therapeutic role of PJM and PHM in oxidative-stress related disorders of kidney and may be helpful in kidney trauma.
    Keywords P. jacquemontiana ; Periploca hydaspidis ; Nephrotoxic ; CCl4 ; DNA ; Comet ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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