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Article ; Online: Assessment of Tobacco Fields for the Prevalence of Verticillium dahliae in District Swabi, Pakistan

Tasiq Ahmad / Said Akram / Shah Fahad / Muhammad Ilyas / Mukhtar Alam / Hazrat Hussain / Latifur Rehman

AGRIVITA Journal of Agricultural Science, Vol 45, Iss 1, Pp 69-

2023 Volume 78

Abstract: Field and laboratory investigations of tobacco plants were carried out to analyze the distribution of Verticillium wilt caused by V. dahliae. V. dahliae is isolated from field Z (Tarkhai, Yarhussain region of Swabi). Species of Fusarium and Actinomycetes ...

Abstract	Field and laboratory investigations of tobacco plants were carried out to analyze the distribution of Verticillium wilt caused by V. dahliae. V. dahliae is isolated from field Z (Tarkhai, Yarhussain region of Swabi). Species of Fusarium and Actinomycetes were detected in field B (Shaheedan, Yarhussain region of Swabi), while no V. dahliae was isolated from these fields. V. dahliae was identified based on its colony and conidia morphology. The colony was white, having oval spores and septate mycelium with phialides. The isolate also produced irregularly elongated dark brown to black color microsclerotia of various sizes. The spores count for V. dahliae isolate was 3.61 × 108 conidia/ml. The average colony diameter of V. dahliae after 9 days of incubation on PDA and CM media was 6.55 cm and 5.5 cm, respectively. The biology of Verticillium spp. in tobacco plants and soil in Swabi has not been studied. For the first time, this study reported the presence of V. dahliae in the tobacco fields of Swabi, KPK Pakistan. Detection and isolation of V. dahliae from field soil are necessary for studying the ecology and virulence and creating deterrent control strategies for Verticillium wilt diseases.
Keywords	microsclerotia ; morphology ; tobacco ; verticillium dahliae ; verticillium wilt ; Agriculture ; S ; Plant culture ; SB1-1110
Subject code	580
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Universitas Brawijaya
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Metastatic Supraclavicular Lymph Nodes among Patients with Lung Carcinoma in a Tertiary Care Centre

Suman Lamichhane / Ajit Thapa / Dinesh Chataut / Sundar Suwal / Mukhtar Alam Ansari / Birendra Kumar Yadav

Journal of Nepal Medical Association, Vol 61, Iss

A Descriptive Cross-sectional Study

2023 Volume 262

Abstract: Introduction: Metastatic spread of lung cancer to supraclavicular lymph nodes is considered distant metastasis for treatment purposes. Detection of supraclavicular lymph node metastasis in patients with lung cancer serves for tissue diagnosis by itself ... ...

Abstract	Introduction: Metastatic spread of lung cancer to supraclavicular lymph nodes is considered distant metastasis for treatment purposes. Detection of supraclavicular lymph node metastasis in patients with lung cancer serves for tissue diagnosis by itself and also helps avoid more invasive biopsy from the primary lung mass itself. Ultrasonography of the lower neck can detect supraclavicular lymph nodes before they are palpable and can also be used for safe sampling of these lymph nodes. The aim of this study was to find out the prevalence of metastatic supraclavicular lymph nodes among patients with lung carcinoma in a tertiary care centre. Methods: A descriptive cross-sectional study done in a tertiary care center, carried out from 15 September 2019 to 14 September 2020. Ethical approval was obtained from the Institutional Review Committee (Reference number: 84(611)E2/076/077). The study was done among 92 patients with biopsy-proven lung cancer (lung mass or supraclavicular lymph node biopsy) who were referred for evaluation, and/or percutaneous transthoracic biopsy. Convenience sampling method was used. Point estimate and 90% Confidence Interval were calculated. Results: Among 92 patients with proven lung cancer, metastatic supraclavicular lymph nodes were seen in 13 patients (14.10%) (8.17- 19.73, 90% Confidence Interval). Among 13 patients with metastatic lymph nodes, 9 (69.23%) had palpable supraclavicular lymph nodes. The majority 11 (84.61%) had round-shaped lymph nodes. All metastatic lymph nodes showed loss of echogenic fatty hilum. A total of 12 (92.30%) metastatic lymph nodes showed a peripheral disorganized pattern of vascularity. Conclusions: The prevalence of metastatic supraclavicular lymph nodes was lower than in similar studies done in international settings.
Keywords	lung cancer ; malignancy ; ultrasonography ; Medicine (General) ; R5-920
Subject code	610 ; 000
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Nepal Medical Association
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Metastatic Supraclavicular Lymph Nodes among Patients with Lung Carcinoma in a Tertiary Care Centre: A Descriptive Cross-sectional Study.

Lamichhane, Suman / Thapa, Ajit / Chataut, Dinesh / Suwal, Sundar / Ansari, Mukhtar Alam / Yadav, Birendra Kumar

JNMA; journal of the Nepal Medical Association

2023 Volume 61, Issue 262, Page(s) 539–542

Abstract	Introduction: Metastatic spread of lung cancer to supraclavicular lymph nodes is considered distant metastasis for treatment purposes. Detection of supraclavicular lymph node metastasis in patients with lung cancer serves for tissue diagnosis by itself and also helps avoid more invasive biopsy from the primary lung mass itself. Ultrasonography of the lower neck can detect supraclavicular lymph nodes before they are palpable and can also be used for safe sampling of these lymph nodes. The aim of this study was to find out the prevalence of metastatic supraclavicular lymph nodes among patients with lung carcinoma in a tertiary care centre. Methods: A descriptive cross-sectional study done in a tertiary care center, carried out from 15 September 2019 to 14 September 2020. Ethical approval was obtained from the Institutional Review Committee (Reference number: 84(611)E2/076/077). The study was done among 92 patients with biopsy-proven lung cancer (lung mass or supraclavicular lymph node biopsy) who were referred for evaluation, and/or percutaneous transthoracic biopsy. Convenience sampling method was used. Point estimate and 90% Confidence Interval were calculated. Results: Among 92 patients with proven lung cancer, metastatic supraclavicular lymph nodes were seen in 13 patients (14.10%) (8.17- 19.73, 90% Confidence Interval). Among 13 patients with metastatic lymph nodes, 9 (69.23%) had palpable supraclavicular lymph nodes. The majority 11 (84.61%) had round-shaped lymph nodes. All metastatic lymph nodes showed loss of echogenic fatty hilum. A total of 12 (92.30%) metastatic lymph nodes showed a peripheral disorganized pattern of vascularity. Conclusions: The prevalence of metastatic supraclavicular lymph nodes was lower than in similar studies done in international settings. Keywords: lung cancer; malignancy; ultrasonography.
MeSH term(s)	Humans ; Tertiary Care Centers ; Cross-Sectional Studies ; Lung Neoplasms/diagnostic imaging ; Lung Neoplasms/pathology ; Lymph Nodes/diagnostic imaging ; Lymph Nodes/pathology ; Carcinoma/pathology ; Lung
Language	English
Publishing date	2023-06-01
Publishing country	Nepal
Document type	Journal Article
ZDB-ID	2209910-4
ISSN	1815-672X ; 0028-2715
ISSN (online)	1815-672X
ISSN	0028-2715
DOI	10.31729/jnma.8188
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Computed tomographic assessment of risk of anterior skull base injury of adult patients in tertiary care centre: a cross-sectional study.

Katwal, Shailendra / Kumar, Ravi Ranjan / Ansari, Mukhtar Alam / Suwal, Sundar / Ghimire, Prasoon

Annals of medicine and surgery (2012)

2023 Volume 85, Issue 12, Page(s) 5892–5898

Abstract: Background and objectives: Functional endoscopic sinus surgery (FESS) carries the risk of anterior skull base injury. Understanding computed tomography of the paranasal sinuses (CT PNS) and anatomical variations is crucial before surgery. Several ... ...

Abstract	Background and objectives: Functional endoscopic sinus surgery (FESS) carries the risk of anterior skull base injury. Understanding computed tomography of the paranasal sinuses (CT PNS) and anatomical variations is crucial before surgery. Several classifications, including Kero's, Gera's, and Thailand-Malaysia-Singapore (TMS), assess the risk of skull base injury. The objective was to determine the risk of anterior skull base injury using CT PNS in adult patients. Methods: A study of 188 patients with head and paranasal sinus pathologies used CT scans to measure olfactory fossa depth, the angle between lamina papyracea and horizontal plane, and the distance from the orbital rim to the cribriform plate. Variations were classified using Kero's, Gera's, and TMS classifications. Results: The study involved 188 individuals aged 18-85, with OF depths ranging from 0.1 to 0.52 cm. Kero's Class I was observed in 82.44% and 81.38% of individuals, while distances from orbital floor to cribriform plate and ethmoidal roof ranged from 1.37 to 2.93 cm. TMS Type I was observed in all individuals, and the angle between lateral lamella of the cribriform plate and cribriform plate ranged from 34° to 85°. Gera's Class II was observed in 77.12% and 84.57% of individuals. Conclusion: CT PNS provides important anatomical information for assessing the risk of skull base injury during FESS. Kero's, Gera's, and TMS classifications can be utilized to evaluate this risk. The study findings provide insights into the variations in olfactory fossa depth, distance measurements, and angle, which can aid in preoperative planning and reducing complications during FESS in Nepalese populations.
Language	English
Publishing date	2023-10-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2745440-X
ISSN	2049-0801
ISSN	2049-0801
DOI	10.1097/MS9.0000000000001362
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Article: Prospective quantitative study: Doppler ultrasound in the evaluation of chronic renal allograft disease and correlation with histopathological finding.

Katwal, Shailendra / Suwal, Sundar / Bhandari, Rajan M / Chataut, Dinesh / Ansari, Mukhtar Alam / Lamichhane, Suman

Annals of medicine and surgery (2012)

2023 Volume 85, Issue 10, Page(s) 4811–4815

Abstract: Background and objectives: Renal allograft biopsy is the gold standard for diagnosing chronic allograft nephropathy, but noninvasive methods are needed to avoid unnecessary biopsies. Doppler ultrasonography, particularly the resistive index (RI), ... ...

Abstract	Background and objectives: Renal allograft biopsy is the gold standard for diagnosing chronic allograft nephropathy, but noninvasive methods are needed to avoid unnecessary biopsies. Doppler ultrasonography, particularly the resistive index (RI), correlates with renal allograft dysfunction. This study aims to assess the relationship between renal sonographic parameters and biochemical parameters in diagnosing graft interstitial fibrosis. Methods: The study evaluated 60 renal allograft recipients for sonographic renal morphological features and Doppler indices. The estimated glomerular filtration rate (eGFR) was calculated, and cortical fibrosis after the biopsy was determined using the Banff score. Continuous variables like mean and SD were calculated, and categorical variables were reported using frequencies and proportions. Associations were examined using independent sample Results: The mean eGFR was 75.23±25.45 ml/min/1.73 m Conclusion: Doppler was able to noninvasively predict allograft fibrosis and could be used as a complementary imaging tool during the follow-up of renal allograft patients. Future research is needed to improve evidence, diagnostic criteria, guidelines, and long-term impact.
Language	English
Publishing date	2023-09-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2745440-X
ISSN	2049-0801
ISSN	2049-0801
DOI	10.1097/MS9.0000000000001251
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Article ; Online: Transgenic Organisms

Mukhtar Alam

Asian Journal of Agricultural Sciences, Vol 3, Iss 5, Pp 366-

An Ethical Dilemmafor Pakistan

2011 Volume 368

Abstract: This study reviews the attitudes and policies vis-à-vis Genetically Modified Organisms (GMOs) in Pakistan’s social and legal context. The study recommends an open and serious debate on bio-ethical issues arising out of the introduction of GMOs from ... ...

Abstract	This study reviews the attitudes and policies vis-à-vis Genetically Modified Organisms (GMOs) in Pakistan’s social and legal context. The study recommends an open and serious debate on bio-ethical issues arising out of the introduction of GMOs from abroad or their indigenous development. All strata of the society needs to be made aware of the issues associated with GMOs; comprehensive legislation, effective regulatory mechanisms and competent institutions needs to be created for exploiting GMOs in an ethical and sustainable manner for the long term prosperity of the masses. A survey of social attitudes also concludes that a vast majority regards religious beliefs as the only framework for setting ethical standards. Hence, it is proposed that religious scholars may be educated on the issues and consulted for input during legislation and formulation of policies. A core group of experts is needed for guiding the legislators, policy makers and the public at large.
Keywords	Bioethics ; GMO’s ; legislation ; pakistan ; transgenics ; Agriculture (General) ; S1-972 ; Agriculture ; S ; DOAJ:Agriculture (General) ; DOAJ:Agriculture and Food Sciences
Subject code	170
Language	English
Publishing date	2011-09-01T00:00:00Z
Publisher	Maxwell Science Publication
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Fetal Ductus Venosus Pulsatility Index and Diameter during Second and Third Trimester of Gestation

Pratit Pokharel / Mukhtar Alam Ansari

Journal of Nepal Medical Association, Vol 56, Iss

2017 Volume 205

Abstract: Introduction: The ductus venosus is a small funnel shaped vessel found posterior to the fetal liver connecting the intra-abdominal umbilical vein and the inferior vena cava. It is one of the three physiological shunts in the fetus. The main objective of ... ...

Abstract	Introduction: The ductus venosus is a small funnel shaped vessel found posterior to the fetal liver connecting the intra-abdominal umbilical vein and the inferior vena cava. It is one of the three physiological shunts in the fetus. The main objective of this study is to construct the reference table and normogram for fetal ductus venosus Pulsatility Index and diameter with gestational age. Methods: This was a prospective cross sectional study conducted during August 2011 to July 2012 taking 294 uncomplicated pregnancies using systemic random sampling method. Three measurements were made for ductus venosus diameter and Pulsatility Index in each fetus during period of fetal quiescence. Results: The ductus venosus diameter at <20weeks, 20-25 weeks, 25-30 weeks, >30 weeks were 1.16, 1.31, 1.62, 1.81 and Pulsatility Index at <20weeks, 20-25 weeks, 25-30 weeks, >30 weeks were 0.41, 0.44, 0.41 and 0.41 respectively. Conclusions: The mean diameter of the ductus venosus was linear across gestational age. The Pulsatility Index shows a scattered distribution across the gestational age. Keywords: ductus venosus diameter; gestational age; Pulsatility Index.
Keywords	ductus venosus diameter ; gestational age ; Pulsatility Index ; Medicine (General) ; R5-920
Subject code	610
Language	English
Publishing date	2017-03-01T00:00:00Z
Publisher	Nepal Medical Association
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Hepatobiliary Imaging and Its Pitfalls

Mukhtar Alam Ansari

Iranian Journal of Radiology, Vol 8, Iss S1, Pp 84-

2011 Volume 84

Abstract: Ultrasound is the initial imaging modality for the"nevaluation of acute right upper quadrant pain. It"npermits accurate diagnosis of acute cholecystitis and"nsuccessfully identifies multiple other causes of patient"nsymptomatology. Some of these ... ...

Abstract	Ultrasound is the initial imaging modality for the"nevaluation of acute right upper quadrant pain. It"npermits accurate diagnosis of acute cholecystitis and"nsuccessfully identifies multiple other causes of patient"nsymptomatology. Some of these processes lie outside"nthe hepatobiliary system and include renal infection"nand obstruction, pancreatitis and its sequelae, duodenal"nor colonic perforation or mass lesions, peritoneal"ntumor spread, adrenal hemorrhage, and even remote"nproblems, such as pneumonia. The limitations on US"ninclude incomplete imaging of the liver, most often at"nthe dome or beneath ribs on the surface, and incomplete"nvisualization of lesion boundaries, particularly"nwith some infections and tumors. For these clinical"nscenarios, contrast-enhanced CT is complementary to"nUS and should be encouraged. In the biliary tree, US"nhas limitations in situations in which the ducts are not"ndilated and sometimes with imaging the extrahepatic"nducts, especially distally. For these patients, CT or"nMR imaging (MRCP) is especially useful. If one keeps"nthe clinical scenario in mind and always images a"npatient where the pain is located, US is a powerful and"neffective diagnostic method for evaluating acute right"nupper quadrant pain.
Keywords	Medical physics. Medical radiology. Nuclear medicine ; R895-920 ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Language	English
Publishing date	2011-05-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Multivariate approach to the classification and ordination of the forest ecosystem of Nandiar valley western Himalayas

Haq, Faizul / Ahmet Aksoy / Habib Ahmad / Mukhtar Alam / Zafar Iqbal

Ecological indicators. 2017 Sept., v. 80

2017

Abstract: Does the vegetation composition of the forests of Nandiar Valley correlate with climatic, topographic and edaphic variables? Is it possible to identify plant communities through indicator species in relation to environmental gradients? Can this approach ... ...

Abstract	Does the vegetation composition of the forests of Nandiar Valley correlate with climatic, topographic and edaphic variables? Is it possible to identify plant communities through indicator species in relation to environmental gradients? Can this approach of classification and ordination will be helpful for conservation planning?Forests of Nandiar Valley, Moist temperate Western Himalayas Pakistan.Eighty stands were selected for quantitative and qualitative characteristic of vegetation between an elevations of 525–3817m. Species composition was recorded by using 400m long transects. GPS, climatic, edaphic and topographic data were recorded for each sampling site. The relationship between habitat types, species composition and distribution along with climatic, edaphic and topographic variables were analyzed using TWINSPAN, Cluster analysis and DCA ordination.Sum 325 vascular plants species belonging to 97 families were recorded. Diversity index and species richness was maximum in the moist temperate zone. Classification and ordination showed that the variance in species data was 7.07. Two-ways indicator species analysis classified the vegetation into eight plant communities. Indicator species analysis revealed that slope aspect, wind speed, temperature, dew point, wet bulb, pH, organic matter and phosphorous were the strongest parameters (p≤0.05) determining plant community composition and indicator species in each habitat. The results also show the strength of the environment – species relationship using Monte Carlo procedures. DCA ordination grouped different species having similar habitat and habitats having common species.The multivariate analysis of the vegetation along with environmental variables of Nandiar valley confirmed the indicators of each sort of vegetation communities/microclimatic zones which could further be used in conservation planning and management not only in studied area but also in the adjacent regions as well as in the areas exhibit similar sort of climatic, edaphic and topographic conditions.
Keywords	botanical composition ; cluster analysis ; dewpoint ; environmental factors ; forest ecosystems ; forests ; global positioning systems ; habitats ; indicator species ; microclimate ; Monte Carlo method ; multivariate analysis ; organic matter ; pH ; phosphorus ; planning ; plant communities ; temperate zones ; temperature ; topography ; variance ; vascular plants ; wind speed
Language	English
Dates of publication	2017-09
Size	p. 232-241.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2036774-0
ISSN	1872-7034 ; 1470-160X
ISSN (online)	1872-7034
ISSN	1470-160X
DOI	10.1016/j.ecolind.2017.05.047
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Monitoring Bioaccumulation (in Gills and Muscle Tissues), Hematology, and Genotoxic Alteration in Ctenopharyngodon idella Exposed to Selected Heavy Metals

Nazish Shah / Ahsan Khan / Riaz Ali / Kasi Marimuthu / Muhammad Nazir Uddin / Muhammad Rizwan / Khaliq Ur Rahman / Mukhtar Alam / Muhammad Adnan / Muhammad / Shahibzada Muhammad Jawad / Saddam Hussain / Muhammad Khisroon

BioMed Research International, Vol

2020 Volume 2020

Abstract: Health and environmental problems arising from metals present in the aquatic ecosystem are very well known. The present study investigated toxicological effects of LC15 of metals such as copper, chromium, and lead for 24, 48, 72, and 96 h on ... ...

Abstract	Health and environmental problems arising from metals present in the aquatic ecosystem are very well known. The present study investigated toxicological effects of LC15 of metals such as copper, chromium, and lead for 24, 48, 72, and 96 h on hematological indices, RBC nucleus and cell morphology, and gill and muscle tissues of grass carp (Ctenopharyngodon idella). Experimental dose concentrations of copper were 1.5, 1.4, 1.2, and 1 mgL−1. Similarly, dose concentrations of chromium were 25.5, 22.5, 20, and 18 mgL−1 while those of lead were 250, 235, 225, and 216 mgL−1, respectively. Maximum decrease in the concentration of Hb, RBCs, and monocytes was observed against chromium, while maximum increase in the concentration of lymphocytes was reported against lead. Abnormalities such as single and double micronuclei, deformed nucleus, nuclear shift, irregular nucleus, deformed cells, microcyte cells, and vacuolated and swollen cells were observed. Gill tissues absorbed maximum concentration of lead followed by chromium and copper. Muscle tissues also absorbed maximum concentration of lead followed by chromium and copper, respectively. Histological alterations such as epithelial lifting, interlamellar spaces, club gill filaments, gill bridging, curling filaments, swelling and fusion of cells, irregular cells, destruction of epithelial cells, cellular necrosis, and inflammatory cells were observed in gill tissues while inflammation and necrosis of muscle fibers, degeneration of muscle fibers, edema of muscle bundles, zig-zag of muscle fibers, and lesions were observed in muscle tissues of fish exposed with different doses of these heavy metals, indicating the toxicity of metals to aquatic fauna as well as to human being via food chain.
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
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