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  1. Article ; Online: The Effect of Mianserin on Lifespan of Caenorhabditis elegan is Abolished by Glucose.

    Almotayri, Abdullah / Thomas, Jency / Munasinghe, Mihiri / Jois, Markandeya

    Current aging science

    2021  Volume 14, Issue 2, Page(s) 118–123

    Abstract: Background: The antidepressant Mianserin has been shown to extend the lifespan of Caenorhabditis elegan (C. elegan), a well-established model organism used in ageing research. The extension of lifespan in C. elegan was shown to be dependent on increased ...

    Abstract Background: The antidepressant Mianserin has been shown to extend the lifespan of Caenorhabditis elegan (C. elegan), a well-established model organism used in ageing research. The extension of lifespan in C. elegan was shown to be dependent on increased expression of the scaffolding protein (ANK3/unc-44). In contrast, antidepressant use in humans is associated with an increased risk of death. The C. elegan elegan in the laboratory are fed Escherichia coli (E. coli), a diet high in protein and low in carbohydrate, whereas a typical human diet is high in carbohydrates. We hypothesized that dietary carbohydrates might mitigate the lifespan-extension effect of mianserin.
    Objective: To investigate the effect of glucose added to the diet of C. elegan on the lifespan-extension effect of mianserin.
    Methods: Wild-type Bristol N2 and ANK3/unc-44 inactivating mutants were cultured on agar plates containing nematode growth medium and fed E. coli. Treatment groups included (C) control, (M50) 50 μM mianserin, (G) 73 mM glucose, and (M50G) 50 μM mianserin and 73 mM glucose. Lifespan was determined by monitoring the worms until they died. Statistical analysis was performed using the Kaplan-Meier version of the log-rank test.
    Results: Mianserin treatment resulted in a 12% increase in lifespan (P<0.05) of wild-type Bristol N2 worms but reduced lifespan by 6% in ANK3/unc-44 mutants, consistent with previous research. The addition of glucose to the diet reduced the lifespan of both strains of worms and abolished the lifespan-extension by mianserin.
    Conclusion: The addition of glucose to the diet of C. elegan abolishes the lifespan-extension effects of mianserin.
    MeSH term(s) Animals ; Caenorhabditis elegans/drug effects ; Caenorhabditis elegans Proteins ; Glucose/pharmacology ; Longevity ; Mianserin/pharmacology
    Chemical Substances Caenorhabditis elegans Proteins ; Mianserin (250PJI13LM) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2021-01-11
    Publishing country United Arab Emirates
    Document type Journal Article
    ISSN 1874-6128
    ISSN (online) 1874-6128
    DOI 10.2174/1874609813999210104203614
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Early Exposure is Necessary for the Lifespan Extension Effects of Cocoa in

    Munasinghe, Mihiri / Almotayri, Abdullah / Thomas, Jency / Heydarian, Deniz / Jois, Markandeya

    Nutrition and metabolic insights

    2021  Volume 14, Page(s) 11786388211029443

    Abstract: Background: We previously showed that cocoa, a rich source of polyphenols improved the age-associated health and extended the lifespan in : Aim: In this study, we aimed to find out the effects of timing of cocoa exposure on longevity improving ... ...

    Abstract Background: We previously showed that cocoa, a rich source of polyphenols improved the age-associated health and extended the lifespan in
    Aim: In this study, we aimed to find out the effects of timing of cocoa exposure on longevity improving effects and the mechanisms and pathways involved in lifespan extension in
    Methods: The standard
    Results: Cocoa exposure starting from L1 stage till the death and till adult day 1 significantly extended the lifespan of worms. However, cocoa supplementation at other larval stages as well as at adulthood could not extend the lifespan, instead the lifespan was significantly reduced. Cocoa could not extend the lifespan of
    Conclusion: Early-start supplementation is essential for cocoa-mediated lifespan extension which is dependent on insulin/IGF-1 signaling pathway and mitochondrial respiration.
    Language English
    Publishing date 2021-07-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2594646-8
    ISSN 1178-6388
    ISSN 1178-6388
    DOI 10.1177/11786388211029443
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Metabolic and behavioral effects of olanzapine and fluoxetine on the model organism

    Almotayri, Abdullah / Thomas, Jency / Munasinghe, Mihiri / Weerasinghe, Maneka / Heydarian, Deniz / Jois, Markandeya

    Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

    2021  Volume 29, Issue 8, Page(s) 917–929

    Abstract: The use of many psychotropic drugs (PDs) is associated with increased caloric intake, significant weight gain, and metabolic disorders. The ... ...

    Abstract The use of many psychotropic drugs (PDs) is associated with increased caloric intake, significant weight gain, and metabolic disorders. The nematode
    Language English
    Publishing date 2021-07-16
    Publishing country Saudi Arabia
    Document type Journal Article
    ZDB-ID 1378024-4
    ISSN 1319-0164
    ISSN 1319-0164
    DOI 10.1016/j.jsps.2021.07.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Β-Carotene content of M. Longifolia seed oil in different agro-climatic zones in Sri Lanka, the effect of heat on its stability and the composition of seed cake

    Munasinghe, Mihiri / Wansapala, Jagath

    Potravinárstvo

    Abstract: M. longifolia is a plant with a seed rich of edible oil (more than 50%), but is still under-utilized for edible purposes in Sri Lankan context. It shows a wide distribution throughout the country representing several agro-climatic zones. No studies have ... ...

    Abstract M. longifolia is a plant with a seed rich of edible oil (more than 50%), but is still under-utilized for edible purposes in Sri Lankan context. It shows a wide distribution throughout the country representing several agro-climatic zones. No studies have done yet to discover variations of M. longifolia seed oil with respect to its different geographical locations. In this study, the content of β-carotene in M. longifolia seed oil samples obtained from four different agro-climatic zones in Sri Lanka was evaluated. The effect of heat on the stability of β-carotene in M. longifolia seed oil was also studied. Dried, fallen seeds were collected from randomly selected trees in four agro-climatic zones in Sri Lanka named low country dry zone (LD), low country wet zone (LW), low country intermediate zone (LI) and mid country intermediate zone (MI). Oil was extracted with a small scale, mechanical oil expeller (cold pressed method). β-carotene content in four samples was assessed with MPOB test method using Ultraviolet-Visible (UV-VIS) spectrophotometer and with High Performance Liquid Chromatography (HPLC) using Chase et al., (1994) method. A series of heat treatment (50 °C - 300 °C) was given and the content of β-carotene was determined at each temperature with the above mentioned two methods. There were some differences in the content of β-carotene for two types of methods. β-carotene content varies from 17.69 to 13.51 ppm in four agro-climatic zones for HPLC method and 20.46 - 27.69 ppm for spectrophotometric method. The reduction of β-carotene content up to 150 °C from the room temperature (30 °C) was not prominent. But after 150 °C, a sudden, sharp decrease was reported. Nutritional composition of seed cake varied significantly (p
    Keywords oil plants ; climate ; carotenes
    Document type Article
    ISSN 1337-0960
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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