Article ; Online: Reanalysis of a μ opioid receptor crystal structure reveals a covalent adduct with BU72.
2023 Volume 21, Issue 1, Page(s) 213
Abstract: Background: The first crystal structure of the active μ opioid receptor (μOR) exhibited several unexplained features. The ligand BU72 exhibited many extreme deviations from ideal geometry, along with unexplained electron density. I previously showed ... ...
Abstract | Background: The first crystal structure of the active μ opioid receptor (μOR) exhibited several unexplained features. The ligand BU72 exhibited many extreme deviations from ideal geometry, along with unexplained electron density. I previously showed that inverting the benzylic configuration resolved these problems, establishing revised stereochemistry of BU72 and its analog BU74. However, another problem remains unresolved: additional unexplained electron density contacts both BU72 and a histidine residue in the N-terminus, revealing the presence of an as-yet unidentified atom. Results: These short contacts and uninterrupted density are inconsistent with non-covalent interactions. Therefore, BU72 and μOR form a covalent adduct, rather than representing two separate entities as in the original model. A subsequently proposed magnesium complex is inconsistent with multiple lines of evidence. However, oxygen fits the unexplained density well. While the structure I propose is tentative, similar adducts have been reported previously in the presence of reactive oxygen species. Moreover, known sources of reactive oxygen species were present: HEPES buffer, nickel ions, and a sequence motif that forms redox-active nickel complexes. This motif contacts the unexplained density. The adduct exhibits severe strain, and the tethered N-terminus forms contacts with adjacent residues. These forces, along with the nanobody used as a G protein substitute, would be expected to influence the receptor conformation. Consistent with this, the intracellular end of the structure differs markedly from subsequent structures of active μOR bound to G Conclusions: Later G |
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MeSH term(s) | Binding Sites ; Ligands ; Receptors, Opioid, mu/chemistry ; Receptors, Opioid, mu/metabolism ; Nickel/metabolism ; Reactive Oxygen Species/metabolism ; GTP-Binding Proteins/metabolism |
Chemical Substances | Ligands ; Receptors, Opioid, mu ; Nickel (7OV03QG267) ; Reactive Oxygen Species ; GTP-Binding Proteins (EC 3.6.1.-) |
Language | English |
Publishing date | 2023-10-10 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 2133020-7 |
ISSN | 1741-7007 ; 1741-7007 |
ISSN (online) | 1741-7007 |
ISSN | 1741-7007 |
DOI | 10.1186/s12915-023-01689-w |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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