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  1. Article ; Online: Specificity and sensitivity of an indirect fluorescent antibody test to detect antibodies against bovine viral diarrhea virus.

    Karakida, Naoya / Murakami, Momoko / Takeda, Yohei / Ogawa, Haruko / Imai, Kunitoshi

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    2024  Volume 36, Issue 2, Page(s) 222–228

    Abstract: Since being reported in 1979 and 2006, indirect fluorescent antibody (IFA) tests have not been reported to detect bovine viral diarrhea virus (BVDV) antibodies to our knowledge. Thus, we re-evaluated the efficacy and usefulness of IFA tests for BVDV ... ...

    Abstract Since being reported in 1979 and 2006, indirect fluorescent antibody (IFA) tests have not been reported to detect bovine viral diarrhea virus (BVDV) antibodies to our knowledge. Thus, we re-evaluated the efficacy and usefulness of IFA tests for BVDV serology. We tested 4 combinations of 2 antibody conjugates (fluorescein isothiocyanate [FITC]-conjugated rabbit IgG anti-bovine IgG; rabbit IgG F(ab')
    MeSH term(s) Cattle ; Animals ; Rabbits ; Fluorescein-5-isothiocyanate ; Diarrhea Viruses, Bovine Viral/genetics ; Diarrhea Virus 1, Bovine Viral/genetics ; Antibodies, Viral ; Immunoglobulin G ; Diarrhea/veterinary ; Bovine Virus Diarrhea-Mucosal Disease/diagnosis ; Cattle Diseases
    Chemical Substances Fluorescein-5-isothiocyanate (I223NX31W9) ; Antibodies, Viral ; Immunoglobulin G
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/10406387241229724
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3645: Show issues Location:
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  2. Article ; Online: Both IRBIT and long-IRBIT bind to and coordinately regulate Cl

    Itoh, Ryo / Hatano, Naoya / Murakami, Momoko / Mitsumori, Kosuke / Kawasaki, Satoko / Wakagi, Tomoka / Kanzaki, Yoshino / Kojima, Hiroyuki / Kawaai, Katsuhiro / Mikoshiba, Katsuhiko / Hamada, Koichi / Mizutani, Akihiro

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 5990

    Abstract: Anion exchanger 2 (AE2) plays crucial roles in regulating cell volume homeostasis and cell migration. We found that both IRBIT and Long-IRBIT (L-IRBIT) interact with anion exchanger 2 (AE2). The interaction occurred between the conserved AHCY-homologous ... ...

    Abstract Anion exchanger 2 (AE2) plays crucial roles in regulating cell volume homeostasis and cell migration. We found that both IRBIT and Long-IRBIT (L-IRBIT) interact with anion exchanger 2 (AE2). The interaction occurred between the conserved AHCY-homologous domain of IRBIT/L-IRBIT and the N-terminal cytoplasmic region of AE2. Interestingly, AE2 activity was reduced in L-IRBIT KO cells, but not in IRBIT KO cells. Moreover, AE2 activity was slightly increased in IRBIT/L-IRBIT double KO cells. These changes in AE2 activity resulted from changes in the AE2 expression level of each mutant cell, and affected the regulatory volume increase and cell migration. The activity and expression level of AE2 in IRBIT/L-IRBIT double KO cells were downregulated if IRBIT, but not L-IRBIT, was expressed again in the cells, and the downregulation was cancelled by the co-expression of L-IRBIT. The mRNA levels of AE2 in each KO cell did not change, and the downregulation of AE2 in L-IRBIT KO cells was inhibited by bafilomycin A1. These results indicate that IRBIT binding facilitates the lysosomal degradation of AE2, which is inhibited by coexisting L-IRBIT, suggesting a novel regulatory mode of AE2 activity through the binding of two homologous proteins with opposing functions.
    MeSH term(s) Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Bicarbonates/metabolism ; Biological Transport ; Cell Line ; Chloride-Bicarbonate Antiporters/chemistry ; Chloride-Bicarbonate Antiporters/metabolism ; Chlorides/metabolism ; Gene Knockdown Techniques ; Humans ; Ion Channel Gating ; Lectins, C-Type/chemistry ; Lectins, C-Type/genetics ; Lectins, C-Type/metabolism ; Lysosomes/metabolism ; Melanoma, Experimental ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Mice ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Isoforms ; Protein Multimerization ; Proteolysis ; Stress, Physiological
    Chemical Substances Bicarbonates ; CLECL1 protein, human ; Chloride-Bicarbonate Antiporters ; Chlorides ; Lectins, C-Type ; Membrane Proteins ; Protein Isoforms
    Language English
    Publishing date 2021-03-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-85499-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Microbeam Wide-Angle X-ray Scattering Study on Precursor of Shish Kebab. Effects of Shear Rate and Annealing on Inner Structure

    Matsuura, Tomohiko / Inoue Rintaro / Kanaya Toshiji / Murakami Momoko / Nishida Koji / Ogawa Hiroki / Ohta Noboru

    Macromolecules. 2015 May 26, v. 48, no. 10

    2015  

    Abstract: We have investigated the inner structure of shear-induced precursor of shish kebab of isotactic polystyrene (iPS) above the nominal melting temperature Tm using microbeam wide-angle X-ray scattering. We first studied the effects of the shear rate on the ... ...

    Abstract We have investigated the inner structure of shear-induced precursor of shish kebab of isotactic polystyrene (iPS) above the nominal melting temperature Tm using microbeam wide-angle X-ray scattering. We first studied the effects of the shear rate on the inner structure and found that the crystallinity in the precursor increased with the shear rate while the crystallite size was independent of that. This was explained in terms of effects of chain entanglements on shear-induced crystallization. We also studied effects of annealing below the nominal melting temperature Tm and found no changes in the crystallites size before and after the annealing, suggesting that the crystallites did not grow into shishs during the annealing below Tm although the kebabs grew.
    Keywords annealing ; crystal structure ; crystallites ; crystallization ; melting point ; polystyrenes ; wide-angle X-ray scattering
    Language English
    Dates of publication 2015-0526
    Size p. 3337-3343.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1491942-4
    ISSN 1520-5835 ; 0024-9297
    ISSN (online) 1520-5835
    ISSN 0024-9297
    DOI 10.1021%2Facs.macromol.5b00372
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Synthesis of 5-tert-butyl-1-(3-tert-butyldimethylsiloxy)phenyl-4,4-dimethyl-2,6,7-trioxabicyclo[3.2.0]heptanes and their fluoride-induced chemiluminescent decomposition: effect of a phenolic electron donor on the CIEEL decay rate in aprotic polar solvent.

    Matsumoto, Masakatsu / Ito, Yoshihiro / Murakami, Momoko / Watanabe, Nobuko

    Luminescence : the journal of biological and chemical luminescence

    2002  Volume 17, Issue 5, Page(s) 305–312

    Abstract: Four bicyclic dioxetanes bearing a phenolic substituent, 3-tert-butyldimethylsiloxy-4-chlorophenyl (3a), 5-tert-butyldimethylsiloxy-4-chloro-2-ethylphenyl (3b), 5-tert-butyldimethylsiloxy-2-ethylphenyl (3c), and 3-tert-butyldimethylsiloxy-4-ethylphenyl ( ... ...

    Abstract Four bicyclic dioxetanes bearing a phenolic substituent, 3-tert-butyldimethylsiloxy-4-chlorophenyl (3a), 5-tert-butyldimethylsiloxy-4-chloro-2-ethylphenyl (3b), 5-tert-butyldimethylsiloxy-2-ethylphenyl (3c), and 3-tert-butyldimethylsiloxy-4-ethylphenyl (3d), were synthesized. All dioxetanes 3a-3d gave intense blue light on treatment with tetrabutylammonium fluoride (TBAF) in DMSO or acetonitrile. Kinetic study on the fluoride-induced CIEEL decay of these dioxetanes 3a-3d and the parent dioxetane 2b revealed that the para-substitution with chlorine on the phenolic moiety of dioxetane increases free energy of activation (DeltaG++), while the para-substitution with ethyl on the aryl decreases DeltaG++. On the other hand, substitution with an ethyl at the ortho-position instead of the para-position was found to increase DeltaG++ and to suppress the CIEEL decay. This fact is attributed to the steric factor of the ortho-ethyl group which would prevent the aromatic ring from rotating freely around the axis joined to the peroxide ring, and supports the suggestion for a CIEEL-active dioxetane bearing a phenolic moiety that an intramolecular electron transfer occurs preferentially from the phenolic donor to O-O of the dioxetane ring, when the aromatic ring lies in a certain conformation(s).
    MeSH term(s) Electrons ; Fluorides ; Heptanes/chemical synthesis ; Luminescent Measurements ; Organosilicon Compounds/chemical synthesis
    Chemical Substances Heptanes ; Organosilicon Compounds ; Fluorides (Q80VPU408O)
    Language English
    Publishing date 2002-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1470995-8
    ISSN 1522-7243 ; 1522-7235 ; 1099-1271
    ISSN (online) 1522-7243
    ISSN 1522-7235 ; 1099-1271
    DOI 10.1002/bio.690
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2962: Show issues Location:
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