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  1. Article: Genetic and physiological requirements for high-level sesquiterpene-production in tomato glandular trichomes.

    Kortbeek, Ruy W J / Galland, Marc D / Muras, Aleksandra / Therezan, Rodrigo / Maia, Sofia / Haring, Michel A / Schuurink, Robert C / Bleeker, Petra M

    Frontiers in plant science

    2023  Volume 14, Page(s) 1139274

    Abstract: Type-VI glandular trichomes of wild ... ...

    Abstract Type-VI glandular trichomes of wild tomato
    Language English
    Publishing date 2023-03-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1139274
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  2. Article ; Online: Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach

    Kortbeek, Ruy W. J. / Galland, Marc D. / Muras, Aleksandra / van der Kloet, Frans M. / André, Bart / Heilijgers, Maurice / van Hijum, Sacha A. F. T. / Haring, Michel A. / Schuurink, Robert C. / Bleeker, Petra M.

    BMC Plant Biol. 2021 Dec., v. 21, no. 1 p.315-315

    2021  

    Abstract: BACKGROUND: Plant-produced specialised metabolites are a powerful part of a plant’s first line of defence against herbivorous insects, bacteria and fungi. Wild ancestors of present-day cultivated tomato produce a plethora of acylsugars in their type-I/IV ...

    Abstract BACKGROUND: Plant-produced specialised metabolites are a powerful part of a plant’s first line of defence against herbivorous insects, bacteria and fungi. Wild ancestors of present-day cultivated tomato produce a plethora of acylsugars in their type-I/IV trichomes and volatiles in their type-VI trichomes that have a potential role in plant resistance against insects. However, metabolic profiles are often complex mixtures making identification of the functionally interesting metabolites challenging. Here, we aimed to identify specialised metabolites from a wide range of wild tomato genotypes that could explain resistance to vector insects whitefly (Bemisia tabaci) and Western flower thrips (Frankliniella occidentalis). We evaluated plant resistance, determined trichome density and obtained metabolite profiles of the glandular trichomes by LC-MS (acylsugars) and GC-MS (volatiles). Using a customised Random Forest learning algorithm, we determined the contribution of specific specialised metabolites to the resistance phenotypes observed. RESULTS: The selected wild tomato accessions showed different levels of resistance to both whiteflies and thrips. Accessions resistant to one insect can be susceptible to another. Glandular trichome density is not necessarily a good predictor for plant resistance although the density of type-I/IV trichomes, related to the production of acylsugars, appears to correlate with whitefly resistance. For type VI-trichomes, however, it seems resistance is determined by the specific content of the glands. There is a strong qualitative and quantitative variation in the metabolite profiles between different accessions, even when they are from the same species. Out of 76 acylsugars found, the random forest algorithm linked two acylsugars (S3:15 and S3:21) to whitefly resistance, but none to thrips resistance. Out of 86 volatiles detected, the sesquiterpene α-humulene was linked to whitefly susceptible accessions instead. The algorithm did not link any specific metabolite to resistance against thrips, but monoterpenes α-phellandrene, α-terpinene and β-phellandrene/D-limonene were significantly associated with susceptible tomato accessions. CONCLUSIONS: Whiteflies and thrips are distinctly targeted by certain specialised metabolites found in wild tomatoes. The machine learning approach presented helped to identify features with efficacy toward the insect species studied. These acylsugar metabolites can be targets for breeding efforts towards the selection of insect-resistant cultivars.
    Keywords Bemisia tabaci ; Frankliniella occidentalis ; algorithms ; cultivars ; herbivores ; insect resistance ; insects ; metabolites ; monoterpenoids ; sesquiterpenoids ; tomatoes ; trichomes
    Language English
    Dates of publication 2021-12
    Size p. 315.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2059868-3
    ISSN 1471-2229
    ISSN 1471-2229
    DOI 10.1186/s12870-021-03070-x
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  3. Article ; Online: Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach.

    Kortbeek, Ruy W J / Galland, Marc D / Muras, Aleksandra / van der Kloet, Frans M / André, Bart / Heilijgers, Maurice / van Hijum, Sacha A F T / Haring, Michel A / Schuurink, Robert C / Bleeker, Petra M

    BMC plant biology

    2021  Volume 21, Issue 1, Page(s) 315

    Abstract: Background: Plant-produced specialised metabolites are a powerful part of a plant's first line of defence against herbivorous insects, bacteria and fungi. Wild ancestors of present-day cultivated tomato produce a plethora of acylsugars in their type-I/ ... ...

    Abstract Background: Plant-produced specialised metabolites are a powerful part of a plant's first line of defence against herbivorous insects, bacteria and fungi. Wild ancestors of present-day cultivated tomato produce a plethora of acylsugars in their type-I/IV trichomes and volatiles in their type-VI trichomes that have a potential role in plant resistance against insects. However, metabolic profiles are often complex mixtures making identification of the functionally interesting metabolites challenging. Here, we aimed to identify specialised metabolites from a wide range of wild tomato genotypes that could explain resistance to vector insects whitefly (Bemisia tabaci) and Western flower thrips (Frankliniella occidentalis). We evaluated plant resistance, determined trichome density and obtained metabolite profiles of the glandular trichomes by LC-MS (acylsugars) and GC-MS (volatiles). Using a customised Random Forest learning algorithm, we determined the contribution of specific specialised metabolites to the resistance phenotypes observed.
    Results: The selected wild tomato accessions showed different levels of resistance to both whiteflies and thrips. Accessions resistant to one insect can be susceptible to another. Glandular trichome density is not necessarily a good predictor for plant resistance although the density of type-I/IV trichomes, related to the production of acylsugars, appears to correlate with whitefly resistance. For type VI-trichomes, however, it seems resistance is determined by the specific content of the glands. There is a strong qualitative and quantitative variation in the metabolite profiles between different accessions, even when they are from the same species. Out of 76 acylsugars found, the random forest algorithm linked two acylsugars (S3:15 and S3:21) to whitefly resistance, but none to thrips resistance. Out of 86 volatiles detected, the sesquiterpene α-humulene was linked to whitefly susceptible accessions instead. The algorithm did not link any specific metabolite to resistance against thrips, but monoterpenes α-phellandrene, α-terpinene and β-phellandrene/D-limonene were significantly associated with susceptible tomato accessions.
    Conclusions: Whiteflies and thrips are distinctly targeted by certain specialised metabolites found in wild tomatoes. The machine learning approach presented helped to identify features with efficacy toward the insect species studied. These acylsugar metabolites can be targets for breeding efforts towards the selection of insect-resistant cultivars.
    MeSH term(s) Algorithms ; Animals ; Disease Resistance/genetics ; Ecotype ; Genetic Variation ; Genotype ; Hemiptera/physiology ; Metabolome/genetics ; Phenotype ; Solanum/genetics ; Thysanoptera/physiology ; Trichomes/genetics ; Trichomes/metabolism ; Volatile Organic Compounds/analysis
    Chemical Substances Volatile Organic Compounds
    Language English
    Publishing date 2021-07-02
    Publishing country England
    Document type Journal Article
    ISSN 1471-2229
    ISSN (online) 1471-2229
    DOI 10.1186/s12870-021-03070-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Reduced seed germination in Arabidopsis over‐expressing SWI/SNF2 ATPase genes

    Leeggangers, Hendrika A. C. F / Folta, Adam / Muras, Aleksandra / Nap, Jan‐Peter / Mlynarova, Ludmila

    Physiologia plantarum. 2015 Feb., v. 153, no. 2

    2015  

    Abstract: In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to ... ...

    Abstract In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over‐expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30% relative to the wild‐type seeds. On the other hand, single loss‐of‐function mutations of the two genes did not affect seed germination. The reduction of germination in over‐expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over‐expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild‐type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.
    Keywords Arabidopsis thaliana ; adenosinetriphosphatase ; chromatin ; epigenetics ; gene overexpression ; genes ; messenger RNA ; metabolism ; mutants ; mutation ; seed germination ; seed maturation ; seeds ; temperature
    Language English
    Dates of publication 2015-02
    Size p. 318-326.
    Publishing place Blackwell Publishing Ltd
    Document type Article
    ZDB-ID 2020837-6
    ISSN 1399-3054 ; 0031-9317
    ISSN (online) 1399-3054
    ISSN 0031-9317
    DOI 10.1111/ppl.12231
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  5. Article ; Online: Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes.

    Leeggangers, Hendrika A C F / Folta, Adam / Muras, Aleksandra / Nap, Jan-Peter / Mlynarova, Ludmila

    Physiologia plantarum

    2015  Volume 153, Issue 2, Page(s) 318–326

    Abstract: In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to ... ...

    Abstract In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over-expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30% relative to the wild-type seeds. On the other hand, single loss-of-function mutations of the two genes did not affect seed germination. The reduction of germination in over-expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over-expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild-type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.
    MeSH term(s) Abscisic Acid/pharmacology ; Adenosine Triphosphatases/genetics ; Adenosine Triphosphatases/metabolism ; Arabidopsis/drug effects ; Arabidopsis/genetics ; Arabidopsis/growth & development ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Chromatin Assembly and Disassembly/drug effects ; Chromatin Assembly and Disassembly/genetics ; Gene Expression Regulation, Plant/drug effects ; Genes, Plant ; Germination/drug effects ; Germination/genetics ; Phenotype ; Plants, Genetically Modified ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Real-Time Polymerase Chain Reaction ; Seeds/drug effects ; Seeds/genetics ; Seeds/growth & development ; Sodium Chloride/pharmacology ; Stress, Physiological/genetics ; Temperature ; Transcription Factors/genetics ; Transcription Factors/metabolism
    Chemical Substances Arabidopsis Proteins ; CHR12 protein, Arabidopsis ; RNA, Messenger ; Transcription Factors ; Sodium Chloride (451W47IQ8X) ; Abscisic Acid (72S9A8J5GW) ; Adenosine Triphosphatases (EC 3.6.1.-) ; CHR23 protein, Arabidopsis (EC 3.6.1.-)
    Language English
    Publishing date 2015-02
    Publishing country Denmark
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020837-6
    ISSN 1399-3054 ; 0031-9317
    ISSN (online) 1399-3054
    ISSN 0031-9317
    DOI 10.1111/ppl.12231
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A growth- and bioluminescence-based bioreporter for the in vivo detection of novel biocatalysts.

    van Rossum, Teunke / Muras, Aleksandra / Baur, Marco J J / Creutzburg, Sjoerd C A / van der Oost, John / Kengen, Servé W M

    Microbial biotechnology

    2017  Volume 10, Issue 3, Page(s) 625–641

    Abstract: The use of bioreporters in high-throughput screening for small molecules is generally laborious and/or expensive. The technology can be simplified by coupling the generation of a desired compound to cell survival, causing only positive cells to stay in ... ...

    Abstract The use of bioreporters in high-throughput screening for small molecules is generally laborious and/or expensive. The technology can be simplified by coupling the generation of a desired compound to cell survival, causing only positive cells to stay in the pool of generated variants. Here, a dual selection/screening system was developed for the in vivo detection of novel biocatalysts. The sensor part of the system is based on the transcriptional regulator AraC, which controls expression of both a selection reporter (LeuB or KmR; enabling growth) for rapid reduction of the initially large library size and a screening reporter (LuxCDABE; causing bioluminescence) for further quantification of the positive variants. Of four developed systems, the best system was the medium copy system with KmR as selection reporter. As a proof of principle, the system was tested for the selection of cells expressing an l-arabinose isomerase derived from mesophilic Escherichia coli or thermophilic Geobacillus thermodenitrificans. A more than a millionfold enrichment of cells with l-arabinose isomerase activity was demonstrated by selection and exclusion of false positives by screening. This dual selection/screening system is an important step towards an improved detection method for small molecules, and thereby for finding novel biocatalysts.
    MeSH term(s) Biosensing Techniques/methods ; Enzymes/analysis ; Escherichia coli/genetics ; Escherichia coli/growth & development ; Escherichia coli/metabolism ; Luminescent Measurements ; Mass Screening/methods ; Sequence Analysis, DNA
    Chemical Substances Enzymes
    Language English
    Publishing date 2017-04-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.12612
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Reduced seed germination in Arabidopsis over‐expressing SWI/SNF2 ATPase genes

    Leeggangers, Hendrika A. C. F. / Folta, Adam / Muras, Aleksandra / Nap, Jan‐Peter / Mlynarova, Ludmila

    Physiologia plantarum

    Volume v. 153,, Issue no. 2

    Abstract: In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to ... ...

    Abstract In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over‐expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30% relative to the wild‐type seeds. On the other hand, single loss‐of‐function mutations of the two genes did not affect seed germination. The reduction of germination in over‐expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over‐expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild‐type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.
    Keywords messenger RNA ; genes ; metabolism ; seed germination ; gene overexpression ; mutation ; temperature ; mutants ; Arabidopsis thaliana ; epigenetics ; seed maturation ; seeds ; adenosinetriphosphatase ; chromatin
    Language English
    Document type Article
    ISSN 0031-9317
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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