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Article: The Putative Role of the Transient Receptor Potential Ion Channel of Vanilloid Type 2 in Red Blood Cell Storage Lesions.

Murciano, Nicoletta / Kaestner, Lars

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

2023 Volume 51, Issue 1, Page(s) 52–54

Language	English
Publishing date	2023-07-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2100848-6
ISSN	1660-3818 ; 1660-3796
ISSN (online)	1660-3818
ISSN	1660-3796
DOI	10.1159/000531282
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Article ; Online: Cardiac competence of the paraxial head mesoderm fades concomitant with a shift towards the head skeletal muscle programme

Alzamrooni, Afnan / Mendes Vieira, Petra / Murciano, Nicoletta / Wolton, Matthew / Schubert, Frank R. / Robson, Samuel C. / Dietrich, Susanne

Developmental Biology. 2023 Sept., v. 501 p.39-59

2023

Abstract: The vertebrate head mesoderm provides the heart, the great vessels, some smooth and most head skeletal muscle, in addition to parts of the skull. It has been speculated that the ability to generate cardiac and smooth muscle is the evolutionary ground- ... ...

Abstract	The vertebrate head mesoderm provides the heart, the great vessels, some smooth and most head skeletal muscle, in addition to parts of the skull. It has been speculated that the ability to generate cardiac and smooth muscle is the evolutionary ground-state of the tissue. However, whether indeed the entire head mesoderm has generic cardiac competence, how long this may last, and what happens as cardiac competence fades, is not clear. Bone morphogenetic proteins (Bmps) are known to promote cardiogenesis. Using 41 different marker genes in the chicken embryo, we show that the paraxial head mesoderm that normally does not engage in cardiogenesis has the ability to respond to Bmp for a long time. However, Bmp signals are interpreted differently at different time points. Up to early head fold stages, the paraxial head mesoderm is able to read Bmps as signal to engage in the cardiac programme; the ability to upregulate smooth muscle markers is retained slightly longer. Notably, as cardiac competence fades, Bmp promotes the head skeletal muscle programme instead. The switch from cardiac to skeletal muscle competence is Wnt-independent as Wnt caudalises the head mesoderm and also suppresses Msc-inducing Bmp provided by the prechordal plate, thus suppressing both the cardiac and the head skeletal muscle programmes. Our study for the first time suggests a specific transition state in the embryo when cardiac competence is replaced by skeletal muscle competence. It sets the stage to unravel the cardiac-skeletal muscle antagonism that is known to partially collapse in heart failure.
Keywords	antagonism ; chick embryos ; head ; heart ; heart failure ; mesoderm ; muscles ; skeletal muscle ; skull ; smooth muscle ; vertebrates ; Vertebrate embryos ; Head mesoderm ; Head skeletal muscle ; Developmental competence ; Bmp ; Wnt ; PHM ; SHF
Language	English
Dates of publication	2023-09
Size	p. 39-59.
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	1114-9
ISSN	1095-564X ; 0012-1606
ISSN (online)	1095-564X
ISSN	0012-1606
DOI	10.1016/j.ydbio.2023.06.005
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Article ; Online: Cardiac competence of the paraxial head mesoderm fades concomitant with a shift towards the head skeletal muscle programme.

Alzamrooni, Afnan / Mendes Vieira, Petra / Murciano, Nicoletta / Wolton, Matthew / Schubert, Frank R / Robson, Samuel C / Dietrich, Susanne

Developmental biology

2023 Volume 501, Page(s) 39–59

Abstract	The vertebrate head mesoderm provides the heart, the great vessels, some smooth and most head skeletal muscle, in addition to parts of the skull. It has been speculated that the ability to generate cardiac and smooth muscle is the evolutionary ground-state of the tissue. However, whether indeed the entire head mesoderm has generic cardiac competence, how long this may last, and what happens as cardiac competence fades, is not clear. Bone morphogenetic proteins (Bmps) are known to promote cardiogenesis. Using 41 different marker genes in the chicken embryo, we show that the paraxial head mesoderm that normally does not engage in cardiogenesis has the ability to respond to Bmp for a long time. However, Bmp signals are interpreted differently at different time points. Up to early head fold stages, the paraxial head mesoderm is able to read Bmps as signal to engage in the cardiac programme; the ability to upregulate smooth muscle markers is retained slightly longer. Notably, as cardiac competence fades, Bmp promotes the head skeletal muscle programme instead. The switch from cardiac to skeletal muscle competence is Wnt-independent as Wnt caudalises the head mesoderm and also suppresses Msc-inducing Bmp provided by the prechordal plate, thus suppressing both the cardiac and the head skeletal muscle programmes. Our study for the first time suggests a specific transition state in the embryo when cardiac competence is replaced by skeletal muscle competence. It sets the stage to unravel the cardiac-skeletal muscle antagonism that is known to partially collapse in heart failure.
MeSH term(s)	Animals ; Chick Embryo ; Mesoderm/metabolism ; Bone Morphogenetic Proteins/metabolism ; Head ; Skull/metabolism ; Muscle, Skeletal/metabolism ; Gene Expression Regulation, Developmental
Chemical Substances	Bone Morphogenetic Proteins
Language	English
Publishing date	2023-06-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1114-9
ISSN	1095-564X ; 0012-1606
ISSN (online)	1095-564X
ISSN	0012-1606
DOI	10.1016/j.ydbio.2023.06.005
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Article: The role of mast cells in cellular modifications evoked by Exendin-4 in treated wounds: a preclinical study.

Paroli, Gaia / Murciano, Nicoletta / Mancini, Caterina / Soldaini, Marta / Rijli, Sarah / DeSiena, Gaetano / Bacci, Stefano

Journal of wound care

2022 Volume 31, Issue 8, Page(s) 701–708

Abstract: Objective: To assess the response of cellular infiltration in wounds treated with Exendin-4.: Method: In this study, 16 mice were used. On each mouse, two wounds were produced, one above the other, in order to study the effects of the various ... ...

Abstract	Objective: To assess the response of cellular infiltration in wounds treated with Exendin-4. Method: In this study, 16 mice were used. On each mouse, two wounds were produced, one above the other, in order to study the effects of the various treatments carried out. The wounds then received an intradermal injection of either saline (20μl; Group 1) or Exendin-4 (Exe4, 62ng; Group 2) in the upper and lower wounds, respectively. The mice were euthanised in order to collect the wounds at time of abrasion (T0), at 48 hours (T1), 96 hours (T2) and 144 hours (T3). The expression of the glucagon-like peptide-1 receptor (GLP-1R) was evaluated by Western blot in wound lysates. Histological and histochemistry methods were applied in cryosections. Results: In T2 and T3 treated wounds, the mast cells degranulation index increased while GLP-1R expression, tumour necrosis factor (TNF Conclusion: We propose that after treatment with Exe4, early activation of mast cells is critical in wound healing acceleration. This is crucial in understanding the potential effect of this drug for viable clinical therapies. Declaration of interest: No potential conflict of interest was reported by the authors.
MeSH term(s)	Animals ; Exenatide/pharmacology ; Exenatide/therapeutic use ; Fibroblasts ; Glucagon-Like Peptide-1 Receptor/metabolism ; Mast Cells/metabolism ; Mice ; Wound Healing
Chemical Substances	Glucagon-Like Peptide-1 Receptor ; Exenatide (9P1872D4OL)
Language	English
Publishing date	2022-08-24
Publishing country	England
Document type	Journal Article
ZDB-ID	1353951-6
ISSN	0969-0700
ISSN	0969-0700
DOI	10.12968/jowc.2022.31.8.701
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Article ; Online: The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells.

Petkova-Kirova, Polina / Murciano, Nicoletta / Iacono, Giulia / Jansen, Julia / Simionato, Greta / Qiao, Min / Van der Zwaan, Carmen / Rotordam, Maria Giustina / John, Thomas / Hertz, Laura / Hoogendijk, Arjan J / Becker, Nadine / Wagner, Christian / Von Lindern, Marieke / Egee, Stephane / Van den Akker, Emile / Kaestner, Lars

International journal of molecular sciences

2024 Volume 25, Issue 3

Abstract: The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood. Here, we use state- ...

Abstract	The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood. Here, we use state-of-the-art biochemical methods to probe for the abundance of the channels in RBCs. Furthermore, we utilize automated patch clamp, based on planar chips, to compare the activity of the two channels in reticulocytes and mature RBCs. In addition to this characterization, we performed membrane potential measurements to demonstrate the effect of channel activity and interplay on the RBC properties. Both the Gárdos channel and Piezo1, albeit their average copy number of activatable channels per cell is in the single-digit range, can be detected through transcriptome analysis of reticulocytes. Proteomics analysis of reticulocytes and mature RBCs could only detect Piezo1 but not the Gárdos channel. Furthermore, they can be reliably measured in the whole-cell configuration of the patch clamp method. While for the Gárdos channel, the activity in terms of ion currents is higher in reticulocytes compared to mature RBCs, for Piezo1, the tendency is the opposite. While the interplay between Piezo1 and Gárdos channel cannot be followed using the patch clamp measurements, it could be proved based on membrane potential measurements in populations of intact RBCs. We discuss the Gárdos channel and Piezo1 abundance, interdependencies and interactions in the context of their proposed physiological and pathophysiological functions, which are the passing of small constrictions, e.g., in the spleen, and their active participation in blood clot formation and thrombosis.
MeSH term(s)	Biological Transport ; Calcium/metabolism ; Erythrocytes/metabolism ; Reticulocytes/metabolism ; Humans ; Intermediate-Conductance Calcium-Activated Potassium Channels/metabolism ; Ion Channels/metabolism
Chemical Substances	Calcium (SY7Q814VUP) ; KCNN4 protein, human ; Intermediate-Conductance Calcium-Activated Potassium Channels ; PIEZO1 protein, human ; Ion Channels
Language	English
Publishing date	2024-01-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25031416
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Article ; Online: A high-throughput electrophysiology assay to study the response of PIEZO1 to mechanical stimulation.

Murciano, Nicoletta / Rotordam, Maria Giustina / Becker, Nadine / Ludlow, Melanie J / Parsonage, Gregory / Darras, Alexis / Kaestner, Lars / Beech, David J / George, Michael / Fertig, Niels / Rapedius, Markus / Brüggemann, Andrea

The Journal of general physiology

2023 Volume 155, Issue 12

Abstract: PIEZO1 channels are mechanically activated cation channels that play a pivotal role in sensing mechanical forces in various cell types. Their dysfunction has been associated with numerous pathophysiological states, including generalized lymphatic ... ...

Abstract	PIEZO1 channels are mechanically activated cation channels that play a pivotal role in sensing mechanical forces in various cell types. Their dysfunction has been associated with numerous pathophysiological states, including generalized lymphatic dysplasia, varicose vein disease, and hereditary xerocytosis. Given their physiological relevance, investigating PIEZO1 is crucial for the pharmaceutical industry, which requires scalable techniques to allow for drug discovery. In this regard, several studies have used high-throughput automated patch clamp (APC) combined with Yoda1, a specific gating modifier of PIEZO1 channels, to explore the function and properties of PIEZO1 in heterologous expression systems, as well as in primary cells. However, a combination of solely mechanical stimulation (M-Stim) and high-throughput APC has not yet been available for the study of PIEZO1 channels. Here, we show that optimization of pipetting parameters of the SyncroPatch 384 coupled with multihole NPC-384 chips enables M-Stim of PIEZO1 channels in high-throughput electrophysiology. We used this approach to explore differences between the response of mouse and human PIEZO1 channels to mechanical and/or chemical stimuli. Our results suggest that applying solutions on top of the cells at elevated pipetting flows is crucial for activating PIEZO1 channels by M-Stim on the SyncroPatch 384. The possibility of comparing and combining mechanical and chemical stimulation in a high-throughput patch clamp assay facilitates investigations on PIEZO1 channels and thereby provides an important experimental tool for drug development.
MeSH term(s)	Humans ; Ion Channels/metabolism ; Mechanotransduction, Cellular/physiology ; High-Throughput Screening Assays ; Electrophysiology
Chemical Substances	Ion Channels ; PIEZO1 protein, human
Language	English
Publishing date	2023-10-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3118-5
ISSN	1540-7748 ; 0022-1295
ISSN (online)	1540-7748
ISSN	0022-1295
DOI	10.1085/jgp.202213132
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Article ; Online: Adverse effects of delta-9-tetrahydrocannabinol on sickle red blood cells.

Hatem, Aline / Esperti, Sofia / Murciano, Nicoletta / Qiao, Min / Giustina Rotordam, Maria / Becker, Nadine / Nader, Elie / Maurer, Felix / Pérès, Laurent / Bouyer, Guillaume / Kaestner, Lars / Connes, Philippe / Egée, Stéphane

American journal of hematology

2023 Volume 98, Issue 12, Page(s) E383–E386

Abstract: THC triggers a pronounced entry of ... ...

Abstract	THC triggers a pronounced entry of Ca
MeSH term(s)	Humans ; Dronabinol/adverse effects ; Erythrocytes ; Anemia, Sickle Cell/drug therapy
Chemical Substances	Dronabinol (7J8897W37S)
Language	English
Publishing date	2023-10-06
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	196767-8
ISSN	1096-8652 ; 0361-8609
ISSN (online)	1096-8652
ISSN	0361-8609
DOI	10.1002/ajh.27109
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Article: Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines

Montalbano, Alberto / Sala, Cesare / Abrardo, Chiara / Murciano, Nicoletta / Jahanfar, Farhad / D'Amico, Massimo / Bertoni, Francesco / Becchetti, Andrea / Arcangeli, Annarosa

Data in Brief. 2021 Feb., v. 34

2021

Abstract: In the present work, applying the whole-cell patch-clamp technique in voltage clamp mode, we have investigated the effects of different drugs, such as riluzole, Psora-4 and Tram-34, on the potassium currents in four human lymphoma cell lines. We focused ... ...

Abstract	In the present work, applying the whole-cell patch-clamp technique in voltage clamp mode, we have investigated the effects of different drugs, such as riluzole, Psora-4 and Tram-34, on the potassium currents in four human lymphoma cell lines. We focused on outward currents mediated by two potassium channels (Kv1.3 and KCa3.1), which are known to play a key physiological role in lymphoid cells. The currents were evoked by voltage ramps ranging from -120 mV to +40 mV and the conductance of the two potassium channels was measured between +20 mV and +40 mV, both in the absence and in the presence of the specific blockers Psora-4 (Kv1.3; 1 µM) and Tram-34 (KCa3.1; 1 µM). The effect of the latter was tested after KCa3.1 channels were activated by riluzole 10 µM. Taken together, these data could be useful as an indication of the functional characteristics of the potassium channels in human lymphomas and represent a starting point for the study of potassium conductance in cellular models of these tumors.
Keywords	electric potential difference ; humans ; lymphoma ; neoplasm cells ; patch-clamp technique ; potassium
Language	English
Dates of publication	2021-02
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2020.106668
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Article: Blue-LED-Light Photobiomodulation of Inflammatory Responses and New Tissue Formation in Mouse-Skin Wounds.

Magni, Giada / Tatini, Francesca / Siena, Gaetano De / Pavone, Francesco S / Alfieri, Domenico / Cicchi, Riccardo / Rossi, Michele / Murciano, Nicoletta / Paroli, Gaia / Vannucci, Clarice / Sistri, Ginevra / Pini, Roberto / Bacci, Stefano / Rossi, Francesca

Life (Basel, Switzerland)

2022 Volume 12, Issue 10

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-10-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life12101564
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Article ; Online: Transient receptor potential channel vanilloid type 2 in red cells of cannabis consumer.

Flormann, Daniel / Qiao, Min / Murciano, Nicoletta / Iacono, Giulia / Darras, Alexis / Hof, Sebastian / Recktenwald, Steffen M / Rotordam, Maria Giustina / Becker, Nadine / Geisel, Jürgen / Wagner, Christian / von Lindern, Marieke / van den Akker, Emile / Kaestner, Lars

American journal of hematology

2022 Volume 97, Issue 5, Page(s) E180–E183

MeSH term(s)	Cannabis ; Humans ; Transient Receptor Potential Channels
Chemical Substances	Transient Receptor Potential Channels
Language	English
Publishing date	2022-02-25
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	196767-8
ISSN	1096-8652 ; 0361-8609
ISSN (online)	1096-8652
ISSN	0361-8609
DOI	10.1002/ajh.26509
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