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Article: Role of TNF-α -308G/A Polymorphism in Bipolar Disorder and its Relationship with Clinical and Demographic Variables.

Akram, Shama / Ali, Moazzam / Mutahir, Zeeshan / Ibad, Nabeel / Sarmad, Sana / Mehboob, Sumaira / Saleem, Mahjabeen

2023 Volume 20, Issue 1-3, Page(s) 60–71

Abstract: Objective: Gene-environment interactions might play a significant role in the development of bipolar disorder (BD). The objective of the current study was to investigate the association between tumor necrosis factor (TNF)-α -308 G/A polymorphism and BD ... ...

Abstract	Objective: Gene-environment interactions might play a significant role in the development of bipolar disorder (BD). The objective of the current study was to investigate the association between tumor necrosis factor (TNF)-α -308 G/A polymorphism and BD and conduct a bioinformatics analysis of the protein-protein network of TNF-α. Gene-environment interactions and the relationship between stressful life events (SLEs) and substance abuse with TNF genotypes and other characteristics were analyzed. Methods: The genomic deoxyribonucleic acid (DNA) of 400 patients with BD and 200 control subjects were extracted and genotyped for TNF-α -308 G/A polymorphism. SLEs and substance abuse were evaluated using the Life Event and Difficulty Schedule (LEDS) and a self-designed substance abuse questionnaire for the events six months prior to the onset of the disease, respectively. Gene-environment interactions were assessed by multiple statistical tools. Bioinformatics analysis of the TNF-α network and its interacting proteins was carried out using STRING and Cytoscape softwares. Results: Genotyping analysis revealed a significant association between TNF-α -308 G/A polymorphism and BD ( Conclusion: TNF-α -308 G/A polymorphism is positively associated with BD. SLEs and substance abuse might trigger the early onset of BD. Proteins identified through bioinformatics analysis might contribute to the TNF-α mediated pathophysiology of BD and can be the potential therapeutic targets.
Language	English
Publishing date	2023-05-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2675366-2
ISSN	2158-8341 ; 2158-8333
ISSN (online)	2158-8341
ISSN	2158-8333
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Tissue engineered periosteum: Fabrication of a gelatin basedtrilayer composite scaffold with biomimetic properties for enhanced bone healing.

Tariq, Sana / Shah, Saqlain A / Hameed, Fareeha / Mutahir, Zeeshan / Khalid, Hamad / Tufail, Asma / Akhtar, Hafsah / Chaudhry, Aqif Anwar / Khan, Ather Farooq

International journal of biological macromolecules

2024 Volume 263, Issue Pt 2, Page(s) 130371

Abstract: The periosteum, a vascularized tissue membrane, is essential in bone regeneration following fractures and bone loss due to some other reasons, yet there exist several research gaps concerning its regeneration. These gaps encompass reduced cellular ... ...

Abstract	The periosteum, a vascularized tissue membrane, is essential in bone regeneration following fractures and bone loss due to some other reasons, yet there exist several research gaps concerning its regeneration. These gaps encompass reduced cellular proliferation and bioactivity, potential toxicity, heightened stiffness of scaffold materials, unfavorable porosity, expensive materials and procedures, and suboptimal survivability or inappropriate degradation rates of the implanted materials. This research used an interdisciplinary approach by forming a new material fabricated through electrospinning for the proposed application as a layer-by-layer tissue-engineered periosteum (TEP). TEP comprises poly(ε-caprolactone) (PCL), PCL/gelatin/magnesium-doped zinc oxide (vascular layer), and gelatin/bioactive glass/COD liver oil (osteoconductive layer). These materials were selected for their diverse properties, when integrated into the scaffold formation, successfully mimic the characteristics of native periosteum. Scanning electron microscopy (SEM) was employed to confirm the trilayer structure of the scaffold and determine the average fiber diameter. In-vitro degradation and swelling studies demonstrated a uniform degradation rate that matches the typical recovery time of periosteum. The scaffold exhibited excellent mechanical properties comparable to natural periosteum. Furthermore, the sustained release kinetics of COD liver oil were observed in the trilayer scaffold. Cell culture results indicated that the three-dimensional topography of the scaffold promoted cell growth, proliferation, and attachment, confirming its non-toxicity, biocompatibility, and bioactivity. This study suggests that the fabricated scaffold holds promise as a potential artificial periosteum for treating periostitis and bone fractures.
MeSH term(s)	Tissue Scaffolds/chemistry ; Gelatin/chemistry ; Periosteum ; Biomimetics ; Cod Liver Oil ; Polyesters/chemistry ; Tissue Engineering/methods
Chemical Substances	Gelatin (9000-70-8) ; Cod Liver Oil (8001-69-2) ; Polyesters
Language	English
Publishing date	2024-02-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.130371
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Article ; Online: Cancer metastasis - tricks of the trade.

Zeeshan, Rabia / Mutahir, Zeeshan

Bosnian journal of basic medical sciences

2017 Volume 17, Issue 3, Page(s) 172–182

Abstract: Decades of cancer research have unraveled genetic, epigenetic and molecular pathways leading to plausible therapeutic targets; many of which hold great promise in improving clinical outcomes. Metastatic tumors become evident early on and are one of the ... ...

Abstract	Decades of cancer research have unraveled genetic, epigenetic and molecular pathways leading to plausible therapeutic targets; many of which hold great promise in improving clinical outcomes. Metastatic tumors become evident early on and are one of the major causes of cancer-related fatalities worldwide. This review depicts the sequential events of cancer metastasis. Genetic and epigenetic heterogeneity influences local tumor cell invasion, intravasation, survival in circulation, extravasation and colonization to distant sites. Each sequential event is associated with heterogeneous tumor microenvironment, gain of competence, unique population of cancer stem cells (CSCs), circulatory pathway, compatible niche and immune system support. A tight regulation of metastasis-promoting mechanisms and, in parallel, evading inhibitory mechanisms contribute to the severity and site of metastasis. A comprehensive understanding of tumor cell fate as an individual entity, as well as in combination with different promoting factors and associated molecular mechanisms, is anticipated in the coming years. This will enable scientists to depict design strategies for targeted cancer therapies.
MeSH term(s)	Animals ; Antineoplastic Agents/therapeutic use ; Humans ; Neoplasm Metastasis/drug therapy ; Neoplasm Metastasis/genetics ; Neoplasm Metastasis/pathology ; Neoplastic Stem Cells
Chemical Substances	Antineoplastic Agents
Language	English
Publishing date	2017-08-20
Publishing country	Bosnia and Herzegovina
Document type	Journal Article ; Review
ZDB-ID	2240029-1
ISSN	1840-4812 ; 1512-8601
ISSN (online)	1840-4812
ISSN	1512-8601
DOI	10.17305/bjbms.2017.1908
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Article ; Online: CRISPR/Cas9 engineering of ERK5 identifies its FAK/PYK2 dependent role in adhesion-mediated cell survival.

Ali, Moazzam / Mutahir, Zeeshan / Riaz, Anjum

Biochemical and biophysical research communications

2019 Volume 513, Issue 1, Page(s) 179–185

Abstract: Extracellular signal-regulated kinase 5 (ERK5) is now considered a key regulator of breast cancer cell proliferation, migration and invasion. It is also implicated in growth factor induced anti-apoptotic signaling. But its contribution to adhesion- ... ...

Abstract	Extracellular signal-regulated kinase 5 (ERK5) is now considered a key regulator of breast cancer cell proliferation, migration and invasion. It is also implicated in growth factor induced anti-apoptotic signaling. But its contribution to adhesion-induced survival signaling is not clear. In the present study, using CRISPR/Cas9 editing, we knocked-out ERK5 expression in several cancer cell lines. Then MDA-MB 231 breast cancer cells lacking ERK5 were used to understand its role in adhesion-mediated cell viability. We demonstrated that ERK5 deficient cells exhibited reduced cell attachment to matrix proteins fibronectin and vitronectin. The adhesion ability of these cells was further reduced upon chemical inhibition of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) by PF 431396. FAK/PYK2 inhibited ERK5 knock-out cells also showed markedly reduced cell-viability and increased apoptotic signaling. This was evident from the detection of cleaved PARP and caspase 9 in these cells. Thus, our data suggests a FAK/PYK2 regulated pro-survival role of ERK5 in response to cell adhesion.
MeSH term(s)	Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; CRISPR-Cas Systems ; Cell Adhesion ; Cell Line, Tumor ; Cell Proliferation ; Cell Survival ; Female ; Focal Adhesion Kinase 1/metabolism ; Focal Adhesion Kinase 2/metabolism ; Humans ; Mitogen-Activated Protein Kinase 7/genetics ; Mitogen-Activated Protein Kinase 7/metabolism
Chemical Substances	Focal Adhesion Kinase 1 (EC 2.7.10.2) ; Focal Adhesion Kinase 2 (EC 2.7.10.2) ; PTK2 protein, human (EC 2.7.10.2) ; PTK2B protein, human (EC 2.7.10.2) ; MAPK7 protein, human (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase 7 (EC 2.7.11.24)
Language	English
Publishing date	2019-04-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2019.03.145
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Article: CRISPR/Cas9 engineering of ERK5 identifies its FAK/PYK2 dependent role in adhesion-mediated cell survival

Ali, Moazzam / Mutahir, Zeeshan / Riaz, Anjum

Biochemical and biophysical research communications. 2019 May 21, v. 513, no. 1

2019

Abstract	Extracellular signal-regulated kinase 5 (ERK5) is now considered a key regulator of breast cancer cell proliferation, migration and invasion. It is also implicated in growth factor induced anti-apoptotic signaling. But its contribution to adhesion-induced survival signaling is not clear. In the present study, using CRISPR/Cas9 editing, we knocked-out ERK5 expression in several cancer cell lines. Then MDA-MB 231 breast cancer cells lacking ERK5 were used to understand its role in adhesion-mediated cell viability. We demonstrated that ERK5 deficient cells exhibited reduced cell attachment to matrix proteins fibronectin and vitronectin. The adhesion ability of these cells was further reduced upon chemical inhibition of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) by PF 431396. FAK/PYK2 inhibited ERK5 knock-out cells also showed markedly reduced cell-viability and increased apoptotic signaling. This was evident from the detection of cleaved PARP and caspase 9 in these cells. Thus, our data suggests a FAK/PYK2 regulated pro-survival role of ERK5 in response to cell adhesion.
Keywords	adhesion ; apoptosis ; breast neoplasms ; caspase-9 ; cell adhesion ; cell lines ; cell proliferation ; cell viability ; engineering ; fibronectins ; mitogen-activated protein kinase ; neoplasm cells ; non-specific protein-tyrosine kinase ; tyrosine
Language	English
Dates of publication	2019-0521
Size	p. 179-185.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2019.03.145
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Article: Avidin-Conjugated Nanofibrillar Cellulose Hydrogel Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts

Leppiniemi, Jenni / Mutahir, Zeeshan / Dulebo, Alexander / Mikkonen, Piia / Nuopponen, Markus / Turkki, Paula / Hytönen, Vesa P.

Biomacromolecules. 2021 Sept. 20, v. 22, no. 10

2021

Abstract: The future success of physiologically relevant three-dimensional (3D) cell/tissue models is dependent on the development of functional biomaterials, which can provide a well-defined 3D environment instructing cellular behavior. To establish a platform to ...

Abstract	The future success of physiologically relevant three-dimensional (3D) cell/tissue models is dependent on the development of functional biomaterials, which can provide a well-defined 3D environment instructing cellular behavior. To establish a platform to produce tailored hydrogels, we conjugated avidin (Avd) to anionic nanofibrillar cellulose (aNFC) and demonstrated the use of the resulting Avd-NFC hydrogel for 3D cell culture, where Avd-NFC allows easy functionalization via biotinylated molecules. Avidin was successfully conjugated to nanocellulose and remained functional, as demonstrated by electrophoresis and titration with fluorescent biotin. Rheological analysis indicated that Avd-NFC retained shear-thinning and gel-forming properties. Topological characterization using AFM revealed the preserved fiber structure and confirmed the binding of biotinylated vitronectin (B-VN) on the fiber surface. The 3D cell culture experiments with mouse embryonic fibroblasts demonstrated the performance of Avd-NFC hydrogels functionalized with biotinylated fibronectin (B-FN) and B-VN. Cells cultured in Avd-NFC hydrogels functionalized with B-FN or B-VN formed matured integrin-mediated adhesions, indicated by phosphorylated focal adhesion kinase. We observed significantly higher cell proliferation rates when biotinylated proteins were bound to the Avd-NFC hydrogel compared to cells cultured in Avd-NFC alone, indicating the importance of the presence of adhesive sites for fibroblasts. The versatile Avd-NFC allows the easy functionalization of hydrogels with virtually any biotinylated molecule and may become widely utilized in 3D cell/tissue culture applications.
Keywords	avidin ; biocompatible materials ; biotin ; cell culture ; cell proliferation ; cellulose ; cellulose nanofibers ; electrophoresis ; fibroblasts ; fibronectins ; fluorescence ; hydrogels ; mice ; non-specific protein-tyrosine kinase ; tissue culture ; titration ; topology
Language	English
Dates of publication	2021-0920
Size	p. 4122-4137.
Publishing place	American Chemical Society
Document type	Article
ISSN	1526-4602
DOI	10.1021/acs.biomac.1c00579
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Article ; Online: Avidin-Conjugated Nanofibrillar Cellulose Hydrogel Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts.

Leppiniemi, Jenni / Mutahir, Zeeshan / Dulebo, Alexander / Mikkonen, Piia / Nuopponen, Markus / Turkki, Paula / Hytönen, Vesa P

Biomacromolecules

2021 Volume 22, Issue 10, Page(s) 4122–4137

Abstract	The future success of physiologically relevant three-dimensional (3D) cell/tissue models is dependent on the development of functional biomaterials, which can provide a well-defined 3D environment instructing cellular behavior. To establish a platform to produce tailored hydrogels, we conjugated avidin (Avd) to anionic nanofibrillar cellulose (aNFC) and demonstrated the use of the resulting Avd-NFC hydrogel for 3D cell culture, where Avd-NFC allows easy functionalization
MeSH term(s)	Animals ; Avidin ; Cellulose ; Fibroblasts ; Fibronectins ; Hydrogels ; Mice ; Vitronectin
Chemical Substances	Fibronectins ; Hydrogels ; Vitronectin ; Avidin (1405-69-2) ; Cellulose (9004-34-6)
Language	English
Publishing date	2021-09-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.1c00579
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A computer aided drug discovery based discovery of lead-like compounds against KDM5A for cancers using pharmacophore modeling and high-throughput virtual screening.

Tariq, Asma / Rehman, Hafiz Muzzammel / Mateen, Rana Muhammad / Ali, Moazzam / Mutahir, Zeeshan / Afzal, Muhammad Sohail / Sajjad, Muhammad / Gul, Roquyya / Saleem, Mahjabeen

Proteins

2021 Volume 90, Issue 3, Page(s) 645–657

Abstract: KDM5A over-expression mediates cancer cell proliferation and promotes resistance toward chemotherapy through epigenetic modifications. As its complete mechanism of action is still unknown, there is no KDM5A specific drug available at clinical level. In ... ...

Abstract	KDM5A over-expression mediates cancer cell proliferation and promotes resistance toward chemotherapy through epigenetic modifications. As its complete mechanism of action is still unknown, there is no KDM5A specific drug available at clinical level. In the current study, lead compounds for KDM5A were determined through pharmacophore modeling and high-throughput virtual screening from Asinex libraries containing 0.5 million compounds. These virtual hits were further evaluated and filtered for ADMET properties. Finally, 726 compounds were used for docking analysis against KDM5A. On the basis of docking score, 10 top-ranked compounds were selected and further evaluated for non-central nervous system (CNS) and CNS drug-like properties. Among these compounds, N-{[(7-Methyl-4-oxo-1,2,3,4-tetrahydrocyclopenta [c] chromen-9-yl) oxy]acetyl}-l-phenylalanine (G-score: -11.363 kcal/mol) was estimated to exhibit non-CNS properties while 2-(3,4-Dimethoxy-phenyl)-7-methoxy-chromen-4-one (G-score: -7.977 kcal/mol) was evaluated as CNS compound. Docked complexes of both compounds were finally selected for molecular dynamic simulation to examine the stability. This study concluded that both these compounds can serve as lead compounds in the quest of finding therapeutic agents against KDM5A associated cancers.
MeSH term(s)	Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Binding Sites ; Computer-Aided Design ; Drug Screening Assays, Antitumor ; High-Throughput Screening Assays ; Ligands ; Molecular Docking Simulation ; Phenylalanine/chemistry ; Phenylalanine/pharmacology ; Protein Binding ; Retinoblastoma-Binding Protein 2/metabolism ; Structure-Activity Relationship ; Thermodynamics
Chemical Substances	Antineoplastic Agents ; Ligands ; Phenylalanine (47E5O17Y3R) ; KDM5A protein, human (EC 1.14.11.-) ; Retinoblastoma-Binding Protein 2 (EC 1.14.11.27)
Language	English
Publishing date	2021-10-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	806683-8
ISSN	1097-0134 ; 0887-3585
ISSN (online)	1097-0134
ISSN	0887-3585
DOI	10.1002/prot.26262
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Zs.A 2291: Show issues

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Article ; Online: Synthesis, in Vitro Cholinesterase Inhibition, Molecular Docking, DFT, and ADME Studies of Novel 1,3,4-Oxadiazole-2-Thiol Derivatives.

Tariq, Sidra / Mutahir, Sadaf / Khan, Muhammad Asim / Mutahir, Zeeshan / Hussain, Safdar / Ashraf, Muhammad / Bao, Xiaofang / Zhou, Baojing / Stark, Christian B W / Khan, Islam Ullah

Chemistry & biodiversity

2022 Volume 19, Issue 8, Page(s) e202200157

Abstract: A series of 1,3,4-oxadiazole-2-thiol derivatives bearing various alkyl or aryl moieties were designed, synthesized, and characterized using modern spectroscopic methods to yield 17 compounds (6a-6q) that were screened for acetylcholinesterase (AChE) and ... ...

Abstract	A series of 1,3,4-oxadiazole-2-thiol derivatives bearing various alkyl or aryl moieties were designed, synthesized, and characterized using modern spectroscopic methods to yield 17 compounds (6a-6q) that were screened for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes in the search for 'lead' compounds for Alzheimer's disease treatment (AD). The compounds 6q, 6p, 6k, 6o, and 6l showed inhibitory capability against AChE and BChE, with IC
MeSH term(s)	Acetylcholinesterase/metabolism ; Alzheimer Disease ; Butyrylcholinesterase/metabolism ; Cholinesterase Inhibitors/chemistry ; Humans ; Molecular Docking Simulation ; Molecular Structure ; Oxadiazoles ; Structure-Activity Relationship ; Sulfhydryl Compounds
Chemical Substances	Cholinesterase Inhibitors ; Oxadiazoles ; Sulfhydryl Compounds ; 1,3,4-oxadiazole (20O2F20OUR) ; Acetylcholinesterase (EC 3.1.1.7) ; Butyrylcholinesterase (EC 3.1.1.8)
Language	English
Publishing date	2022-07-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2139001-0
ISSN	1612-1880 ; 1612-1872
ISSN (online)	1612-1880
ISSN	1612-1872
DOI	10.1002/cbdv.202200157
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Article ; Online: Recombinant deoxyribonucleoside kinase from Drosophila melanogaster can improve gemcitabine based combined gene/chemotherapy for targeting cancer cells.

Fatima, Mahak / Iqbal Ahmed, Muhammad Mubashar / Batool, Faiza / Riaz, Anjum / Ali, Moazzam / Munch-Petersen, Birgitte / Mutahir, Zeeshan

Bosnian journal of basic medical sciences

2019 Volume 19, Issue 4, Page(s) 342–349

Abstract: A recombinant deoxyribonucleoside kinase from Drosophila melanogaster with a deletion of the last 20 amino acid residues (named DmdNKΔC20) was hypothesized as a potential therapeutic tool for gene therapy due to its broad substrate specificity and better ...

Abstract	A recombinant deoxyribonucleoside kinase from Drosophila melanogaster with a deletion of the last 20 amino acid residues (named DmdNKΔC20) was hypothesized as a potential therapeutic tool for gene therapy due to its broad substrate specificity and better catalytic efficiency towards nucleosides and nucleoside analogs. This study was designed to evaluate the effect of DmdNKΔC20 for sensitizing human cancer cell lines to gemcitabine and to further investigate its role in reversal of acquired drug resistance in gemcitabine-resistant cancer cell line. The DmdNKΔC20 gene was delivered to three different cancer cell lines, including breast, colon and liver cancer cells, using lipid-mediated transfection reagent. After transfection, gene expression of DmdNKΔC20 was confirmed by quantitative reverse transcription PCR (qRT-PCR) and the combined effect of DmdNKΔC20 and gemcitabine based cytotoxicity was observed by cell viability assay. We further evolved a gemcitabine-resistant breast cancer cell line (named MCF7-R) through directed evolution in the laboratory, which showed 375-fold more resistance compared with parental MCF7 cells. Upon transfection with DmdNKΔC20 gene, MCF7-R cells showed 83-fold higher sensitivity to gemcitabine compared with the control group of MCF7-R cells. Moreover, we observed 79% higher expression of p21 protein in transfected MCF7-R cells, which may indicate induction of apoptosis. Our findings highlight the importance and therapeutic potential of DmdNKΔC20 in combined gene/chemotherapy approach to target a wide range of cancers, particularly gemcitabine-resistant cancers.
MeSH term(s)	Animals ; Apoptosis ; Breast Neoplasms/drug therapy ; Breast Neoplasms/metabolism ; Cell Line, Tumor ; Cell Survival ; Colonic Neoplasms/drug therapy ; Colonic Neoplasms/metabolism ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; Deoxycytidine/analogs & derivatives ; Deoxycytidine/pharmacology ; Drosophila melanogaster ; Drug Resistance, Neoplasm ; Drug Therapy, Combination ; Female ; Genetic Therapy ; Genetic Vectors ; HCT116 Cells ; Humans ; Inhibitory Concentration 50 ; Liver Neoplasms/drug therapy ; Liver Neoplasms/metabolism ; MCF-7 Cells ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Phosphotransferases (Alcohol Group Acceptor)/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Substrate Specificity ; Transfection
Chemical Substances	CDKN1A protein, human ; Cyclin-Dependent Kinase Inhibitor p21 ; Recombinant Proteins ; Deoxycytidine (0W860991D6) ; gemcitabine (B76N6SBZ8R) ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-) ; deoxyribonucleoside kinases (EC 2.7.1.-)
Language	English
Publishing date	2019-11-08
Publishing country	Bosnia and Herzegovina
Document type	Journal Article
ZDB-ID	2240029-1
ISSN	1840-4812 ; 1512-8601
ISSN (online)	1840-4812
ISSN	1512-8601
DOI	10.17305/bjbms.2019.4136
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