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  1. Book ; Online: Endometrial Cancer: From Biological to Clinical Approaches

    Nabissi, Massimo / Amant, Frederic / Gehrig, Paola

    2019  

    Keywords Medicine ; Oncology ; Endometrial cancer ; therapy ; biomarkers ; metabolism ; adjuvant therapies ; miRNA ; bio-informatic analysis ; anti-estrogen therapy ; cytology
    Size 1 electronic resource (157 pages)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021230479
    ISBN 9782889630493 ; 2889630498
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Cannabigerol Induces Autophagic Cell Death by Inhibiting EGFR-RAS Pathways in Human Pancreatic Ductal Adenocarcinoma Cell Lines.

    Zeppa, Laura / Aguzzi, Cristina / Morelli, Maria Beatrice / Marinelli, Oliviero / Giangrossi, Martina / Luongo, Margherita / Amantini, Consuelo / Santoni, Giorgio / Nabissi, Massimo

    International journal of molecular sciences

    2024  Volume 25, Issue 4

    Abstract: Pancreatic ductal adenocarcinoma (PDAC) is the most frequent infiltrating type of pancreatic cancer. The poor prognosis associated with this cancer is due to the absence of specific biomarkers, aggressiveness, and treatment resistance. PDAC is a deadly ... ...

    Abstract Pancreatic ductal adenocarcinoma (PDAC) is the most frequent infiltrating type of pancreatic cancer. The poor prognosis associated with this cancer is due to the absence of specific biomarkers, aggressiveness, and treatment resistance. PDAC is a deadly malignancy bearing distinct genetic alterations, the most common being those that result in cancer-causing versions of the KRAS gene. Cannabigerol (CBG) is a non-psychomimetic cannabinoid with anti-inflammatory properties. Regarding the anticancer effect of CBG, up to now, there is only limited evidence in human cancers. To fill this gap, we investigated the effects of CBG on the PDAC cell lines, PANC-1 and MIAPaCa-2. The effect of CBG activity on cell viability, cell death, and EGFR-RAS-associated signaling was investigated. Moreover, the potential synergistic effect of CBG in combination with gemcitabine (GEM) and paclitaxel (PTX) was investigated. MTT was applied to investigate the effect of CBG on PDAC cell line viabilities. Annexin-V and Acridine orange staining, followed by cytofluorimetric analysis and Western blotting, were used to evaluate CBG's effect on cell death. The modulation of EGFR-RAS-associated pathways was determined by Western blot analysis and a Milliplex multiplex assay. Moreover, by employing the MTT data and SynergyFinder Plus software analysis, the effect of the combination of CBG and chemotherapeutic drugs was determined.
    MeSH term(s) Humans ; Apoptosis ; Autophagic Cell Death/drug effects ; Cannabinoids/pharmacology ; Carcinoma, Pancreatic Ductal/drug therapy ; Carcinoma, Pancreatic Ductal/genetics ; Carcinoma, Pancreatic Ductal/metabolism ; Cell Line, Tumor ; Deoxycytidine/pharmacology ; Deoxycytidine/therapeutic use ; ErbB Receptors/antagonists & inhibitors ; Pancreatic Neoplasms/metabolism ; Proto-Oncogene Proteins p21(ras)/antagonists & inhibitors
    Chemical Substances cannabigerol (J1K406072N) ; Cannabinoids ; Deoxycytidine (0W860991D6) ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1) ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2)
    Language English
    Publishing date 2024-02-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25042001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Calcium influx, oxidative stress, and apoptosis induced by TRPV1 in chronic myeloid leukemia cells: Synergistic effects with imatinib.

    Maggi, Federica / Morelli, Maria Beatrice / Aguzzi, Cristina / Zeppa, Laura / Nabissi, Massimo / Polidori, Carlo / Santoni, Giorgio / Amantini, Consuelo

    Frontiers in molecular biosciences

    2023  Volume 10, Page(s) 1129202

    Abstract: Introduction: ...

    Abstract Introduction:
    Language English
    Publishing date 2023-02-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2814330-9
    ISSN 2296-889X
    ISSN 2296-889X
    DOI 10.3389/fmolb.2023.1129202
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Encapsulation of Hemp (

    Aguzzi, Cristina / Perinelli, Diego Romano / Cespi, Marco / Zeppa, Laura / Mazzara, Eugenia / Maggi, Filippo / Petrelli, Riccardo / Bonacucina, Giulia / Nabissi, Massimo

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 18

    Abstract: Industrial hemp ( ...

    Abstract Industrial hemp (
    MeSH term(s) Cannabis ; Oils, Volatile/pharmacology ; Cannabinoids/pharmacology ; Terpenes
    Chemical Substances Oils, Volatile ; Cannabinoids ; Terpenes
    Language English
    Publishing date 2023-09-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28186479
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.MO 81: Show issues

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  5. Article ; Online: TRPML2 Mucolipin Channels Drive the Response of Glioma Stem Cells to Temozolomide and Affect the Overall Survival in Glioblastoma Patients.

    Morelli, Maria Beatrice / Nabissi, Massimo / Amantini, Consuelo / Maggi, Federica / Ricci-Vitiani, Lucia / Pallini, Roberto / Santoni, Giorgio

    International journal of molecular sciences

    2022  Volume 23, Issue 23

    Abstract: The survival of patients with glioblastoma (GBM) is poor. The main cause is the presence of glioma stem cells (GSCs), exceptionally resistant to temozolomide (TMZ) treatment. This last may be related to the heterogeneous expression of ion channels, among ...

    Abstract The survival of patients with glioblastoma (GBM) is poor. The main cause is the presence of glioma stem cells (GSCs), exceptionally resistant to temozolomide (TMZ) treatment. This last may be related to the heterogeneous expression of ion channels, among them TRPML2. Its mRNA expression was evaluated in two different neural stem cell (NS/PC) lines and sixteen GBM stem-like cells by qRT-PCR. The response to TMZ was evaluated in undifferentiated or differentiated GSCs, and in TRPML2-induced or silenced GSCs. The relationship between TRPML2 expression and responsiveness to TMZ treatment was evaluated by MTT assay showing that increased TRPML2 mRNA levels are associated with resistance to TMZ. This research was deepened by qRT-PCR and western blot analysis. PI3K/AKT and JAK/STAT pathways as well as ABC and SLC drug transporters were involved. Finally, the relationship between TRPML2 expression and overall survival (OS) and progression-free survival (PFS) in patient-derived GSCs was evaluated by Kaplan-Meier analysis. The expression of TRPML2 mRNA correlates with worse OS and PFS in GBM patients. Thus, the expression of TRPML2 in GSCs influences the responsiveness to TMZ in vitro and affects OS and PFS in GBM patients.
    Language English
    Publishing date 2022-12-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232315356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Coexpression of TRPML1 and TRPML2 Mucolipin Channels Affects the Survival of Glioblastoma Patients.

    Santoni, Giorgio / Maggi, Federica / Amantini, Consuelo / Arcella, Antonietta / Marinelli, Oliviero / Nabissi, Massimo / Santoni, Matteo / Morelli, Maria Beatrice

    International journal of molecular sciences

    2022  Volume 23, Issue 14

    Abstract: Among brain cancers, glioblastoma (GBM) is the most malignant glioma with an extremely poor prognosis. It is characterized by high cell heterogeneity, which can be linked to its high malignancy. We have previously demonstrated that TRPML1 channels affect ...

    Abstract Among brain cancers, glioblastoma (GBM) is the most malignant glioma with an extremely poor prognosis. It is characterized by high cell heterogeneity, which can be linked to its high malignancy. We have previously demonstrated that TRPML1 channels affect the OS of GBM patients. Herein, by RT-PCR, FACS and Western blot, we demonstrated that TRPML1 and TRPML2 channels are differently expressed in GBM patients and cell lines. Moreover, these channels partially colocalized in ER and lysosomal compartments in GBM cell lines, as evaluated by confocal analysis. Interestingly, the silencing of TRPML1 or TRPML2 by RNA interference results in the decrease in the other receptor at protein level. Moreover, the double knockdown of TRPML1 and TRPML2 leads to increased GBM cell survival with respect to single-channel-silenced cells, and improves migration and invasion ability of U251 cells. Finally, the Kaplan-Meier survival analysis demonstrated that patients with high TRPML2 expression in absence of TRPML1 expression strongly correlates with short OS, whereas high TRPML1 associated with low TRPML2 mRNA expression correlates with longer OS in GBM patients. The worst OS in GBM patients is associated with the loss of both TRPML1 and TRPML2 channels.
    MeSH term(s) Brain Neoplasms/genetics ; Cell Line ; Glioblastoma/genetics ; Glioma ; Humans ; Transient Receptor Potential Channels/genetics ; Transient Receptor Potential Channels/metabolism
    Chemical Substances Transient Receptor Potential Channels
    Language English
    Publishing date 2022-07-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23147741
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: The effects of cannabidiol via TRPV2 channel in chronic myeloid leukemia cells and its combination with imatinib.

    Maggi, Federica / Morelli, Maria Beatrice / Tomassoni, Daniele / Marinelli, Oliviero / Aguzzi, Cristina / Zeppa, Laura / Nabissi, Massimo / Santoni, Giorgio / Amantini, Consuelo

    Cancer science

    2022  Volume 113, Issue 4, Page(s) 1235–1249

    Abstract: Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by accumulation of immature cells in bone marrow and peripheral blood. Although successful results were obtained with tyrosine kinase inhibitors, several patients showed ... ...

    Abstract Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by accumulation of immature cells in bone marrow and peripheral blood. Although successful results were obtained with tyrosine kinase inhibitors, several patients showed resistance. For this reason, the identification of new strategies and therapeutic biomarkers represents an attractive goal. The role of transient receptor potential (TRP) ion channels as possible drug targets has been elucidated in different types of cancer. Among natural compounds known to activate TRPs, cannabidiol (CBD) displays anticancer properties. By using FACS analysis, confocal microscopy, gene silencing, and cell growth assay, we demonstrated that CBD, through TRPV2, inhibits cell proliferation and cell cycle in CML cells. It promoted mitochondria dysfunction and mitophagy as shown by mitochondrial mass reduction and up-regulation of several mitophagy markers. These effects were associated with changes in the expression of octamer-binding transcription factor 4 and PU.1 markers regulated during cellular differentiation. Interestingly, a synergistic effect by combining CBD with the standard drug imatinib was found and imatinib-resistant cells remain susceptible to CBD effects. Therefore, the targeting of TRPV2 by using CBD, through the activation of mitophagy and the reduction in stemness, could be a promising strategy to enhance conventional therapy and improve the prognosis of CML patients.
    MeSH term(s) Apoptosis ; Cannabidiol/pharmacology ; Cannabidiol/therapeutic use ; Cell Proliferation ; Drug Resistance, Neoplasm ; Humans ; Imatinib Mesylate/pharmacology ; Imatinib Mesylate/therapeutic use ; K562 Cells ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics ; TRPV Cation Channels/genetics ; TRPV Cation Channels/metabolism
    Chemical Substances TRPV Cation Channels ; TRPV2 protein, human ; Cannabidiol (19GBJ60SN5) ; Imatinib Mesylate (8A1O1M485B)
    Language English
    Publishing date 2022-03-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2115647-5
    ISSN 1349-7006 ; 1349-7006
    ISSN (online) 1349-7006
    ISSN 1349-7006
    DOI 10.1111/cas.15257
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Functional In Vitro Assessment of VEGFA/NOTCH2 Signaling Pathway and pRB Proteasomal Degradation and the Clinical Relevance of Mucolipin TRPML2 Overexpression in Glioblastoma Patients.

    Santoni, Giorgio / Amantini, Consuelo / Nabissi, Massimo / Arcella, Antonietta / Maggi, Federica / Santoni, Matteo / Morelli, Maria Beatrice

    International journal of molecular sciences

    2022  Volume 23, Issue 2

    Abstract: Glioblastoma (GBM) is the most malignant glioma with an extremely poor prognosis. It is characterized by high vascularization and its growth depends on the formation of new blood vessels. We have previously demonstrated that TRPML2 mucolipin channel ... ...

    Abstract Glioblastoma (GBM) is the most malignant glioma with an extremely poor prognosis. It is characterized by high vascularization and its growth depends on the formation of new blood vessels. We have previously demonstrated that TRPML2 mucolipin channel expression increases with the glioma pathological grade. Herein by ddPCR and Western blot we found that the silencing of TRPML2 inhibits expression of the VEGFA/Notch2 angiogenic pathway. Moreover, the VEGFA/Notch2 expression increased in T98 and U251 cells stimulated with the TRPML2 agonist, ML2-SA1, or by enforced-TRPML2 levels. In addition, changes in TRPML2 expression or ML2-SA1-induced stimulation, affected Notch2 activation and VEGFA release. An increased invasion capability, associated with a reduced VEGF/VEGFR2 expression and increased vimentin and CD44 epithelial-mesenchymal transition markers in siTRPML2, but not in enforced-TRPML2 or ML2-SA1-stimulated glioma cells, was demonstrated. Furthermore, an increased sensitivity to Doxorubicin cytotoxicity was demonstrated in siTRPML2, whereas ML2-SA1-treated GBM cells were more resistant. The role of proteasome in Cathepsin B-dependent and -independent pRB degradation in siTRPML2 compared with siGLO cells was studied. Finally, through Kaplan-Meier analysis, we found that high TRPML2 mRNA expression strongly correlates with short survival in GBM patients, supporting TRPML2 as a negative prognostic factor in GBM patients.
    MeSH term(s) Cathepsin B/metabolism ; Cell Line, Tumor ; Cell Movement/drug effects ; Cell Movement/genetics ; Doxorubicin/pharmacology ; Drug Resistance, Neoplasm/drug effects ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic/drug effects ; Gene Silencing/drug effects ; Glioblastoma/genetics ; Glioblastoma/metabolism ; Glioblastoma/pathology ; Humans ; Neoplasm Invasiveness ; Phosphorylation/drug effects ; Prognosis ; Proteasome Endopeptidase Complex/metabolism ; Proteolysis/drug effects ; Receptor, Notch2/metabolism ; Retinoblastoma Protein/metabolism ; Signal Transduction/drug effects ; Transient Receptor Potential Channels/genetics ; Transient Receptor Potential Channels/metabolism ; Vascular Endothelial Growth Factor A/metabolism ; Vascular Endothelial Growth Factor Receptor-2/metabolism
    Chemical Substances Mcoln2 protein, human ; NOTCH2 protein, human ; Receptor, Notch2 ; Retinoblastoma Protein ; Transient Receptor Potential Channels ; Vascular Endothelial Growth Factor A ; Doxorubicin (80168379AG) ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1) ; Cathepsin B (EC 3.4.22.1) ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
    Language English
    Publishing date 2022-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23020688
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Pathophysiological Role of Transient Receptor Potential Mucolipin Channel 1 in Calcium-Mediated Stress-Induced Neurodegenerative Diseases.

    Santoni, Giorgio / Maggi, Federica / Amantini, Consuelo / Marinelli, Oliviero / Nabissi, Massimo / Morelli, Maria Beatrice

    Frontiers in physiology

    2020  Volume 11, Page(s) 251

    Abstract: Mucolipins (TRPML) are endosome/lysosome ... ...

    Abstract Mucolipins (TRPML) are endosome/lysosome Ca
    Language English
    Publishing date 2020-03-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2020.00251
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Involvement of the TRPML Mucolipin Channels in Viral Infections and Anti-viral Innate Immune Responses.

    Santoni, Giorgio / Morelli, Maria Beatrice / Amantini, Consuelo / Nabissi, Massimo / Santoni, Matteo / Santoni, Angela

    Frontiers in immunology

    2020  Volume 11, Page(s) 739

    Abstract: The TRPML channels (TRPML1, TRPML2, and TRPML3), belonging to the mucolipin TRP subfamily, primary localize to a population of membrane-bonded vesicles along the endocytosis, and exocytosis pathways. Human viruses enter host cells by plasma membrane ... ...

    Abstract The TRPML channels (TRPML1, TRPML2, and TRPML3), belonging to the mucolipin TRP subfamily, primary localize to a population of membrane-bonded vesicles along the endocytosis, and exocytosis pathways. Human viruses enter host cells by plasma membrane penetration or by receptor-mediated endocytosis. TRPML2 enhances the infectivity of a number of enveloped viruses by promoting virus vesicular trafficking and escape from endosomal compartment. TRPML2 expression is stimulated by interferon and by several toll like receptor (TLR) activators, suggesting a possible role in the activation of the innate immune response. Noteworthy, TRPML1 plays a major role in single strand RNA/DNA trafficking into lysosomes and the lack of TRPML1 impairs the TLR-7 and TLR-9 ligand transportation to lysosomes resulting in decreased dendritic cell maturation/activation and migration to the lymph nodes. TRPML channels are also expressed by natural killer (NK) cells, a subset of innate lymphocytes with an essential role during viral infections; recent findings have indicated a role of TRPML1-mediated modulation of secretory lysosomes in NK cells education. Moreover, as also NK cells express TLR recognizing viral pattern, an increased TLR-mediated activation of cytokine production can be envisaged, suggesting a dual role in the NK cell-mediated antiviral responses. Overall, TRPML channels might play a double-edged sword in resistance to viral infections: on one side they can promote virus cellular entry and infectivity; on the other side, by regulating TLR responses in the various immune cells, they contribute to enhance antiviral innate and possibly adaptive immune responses.
    MeSH term(s) Animals ; Biological Transport ; Cell Membrane/metabolism ; Endocytosis ; Endosomes/metabolism ; Humans ; Immunity, Innate/physiology ; Killer Cells, Natural ; Lysosomes/metabolism ; Mice ; Toll-Like Receptors ; Transient Receptor Potential Channels/metabolism ; Virus Diseases/metabolism
    Chemical Substances MCOLN1 protein, human ; MCOLN3 protein, human ; Mcoln2 protein, human ; Toll-Like Receptors ; Transient Receptor Potential Channels
    Language English
    Publishing date 2020-04-29
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2020.00739
    Database MEDical Literature Analysis and Retrieval System OnLINE

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