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  1. AU="Nackers, Elke"
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  1. Article ; Online: Hemoglobin S monitoring on TOSOH G8 in hemoglobin A1c mode in case of urgent red blood cell exchange.

    Van Aelst, Sophie / Claerhout, Helena / Nackers, Elke / Desmet, Koen / Kieffer, Davy

    Journal of clinical laboratory analysis

    2018  Volume 32, Issue 7, Page(s) e22453

    Abstract: Background: Pre- and post-transfusion hemoglobin S (HbS) levels are used to document the efficacy of red blood cell exchange (RCE) in patients with sickle cell disease (SCD). In case of urgent RCE a 24/7 short turn-around time (STAT) analysis, with the ... ...

    Abstract Background: Pre- and post-transfusion hemoglobin S (HbS) levels are used to document the efficacy of red blood cell exchange (RCE) in patients with sickle cell disease (SCD). In case of urgent RCE a 24/7 short turn-around time (STAT) analysis, with the ability to identify and quantify HbS, is warranted. The use of TOSOH G8 (Tosoh Europe) is evaluated for this purpose, using the variant HbA1c mode.
    Methods: Analytical performance of the HbS analysis on TOSOH G8 in variant HbA1c mode was evaluated, including assessment of imprecision and linearity for HbS. In addition, a comparison study between TOSOH G8 and Minicap Flex Piercing (FP) system CZE (Sebia) using 32 HbS samples (HbS range: 9%-93%) was carried out to evaluate analytical and clinical concordance.
    Results: Total HbS imprecision was 1.77% and 0.31% for a sickle cell trait and a sickle cell anemia sample, respectively. An acceptable linearity (HbS range: 6%-88%) was observed (R
    Conclusion: Hemoglobin S results obtained with TOSOH G8 in variant HbA1c mode are clinically acceptable to monitor urgent RCE. The observed underestimation will not alter clinical decision-making.
    MeSH term(s) Anemia, Sickle Cell/epidemiology ; Anemia, Sickle Cell/therapy ; Erythrocyte Transfusion ; Glycated Hemoglobin A/analysis ; Hemoglobin, Sickle/analysis ; Hemoglobinometry/methods ; Hemoglobinometry/standards ; Humans ; Linear Models ; Reproducibility of Results ; Time Factors
    Chemical Substances Glycated Hemoglobin A ; Hemoglobin, Sickle
    Language English
    Publishing date 2018-04-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645095-7
    ISSN 1098-2825 ; 0887-8013
    ISSN (online) 1098-2825
    ISSN 0887-8013
    DOI 10.1002/jcla.22453
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2471: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: The C6 Lyme antibody test has low sensitivity for antibody detection in cerebrospinal fluid.

    Vermeersch, Pieter / Resseler, Sara / Nackers, Elke / Lagrou, Katrien

    Diagnostic microbiology and infectious disease

    2009  Volume 64, Issue 3, Page(s) 347–349

    Abstract: Our aim was to evaluate the performance of the commercial Immunetics C6 Lyme ELISA assay as a screening assay for anti-Borrelia burgdorferi antibodies in cerebrospinal fluid (CSF). Sensitivity of C6 ELISA was determined in 28 consecutive patients who ... ...

    Abstract Our aim was to evaluate the performance of the commercial Immunetics C6 Lyme ELISA assay as a screening assay for anti-Borrelia burgdorferi antibodies in cerebrospinal fluid (CSF). Sensitivity of C6 ELISA was determined in 28 consecutive patients who were diagnosed with neuroborreliosis and had evidence for intrathecal antibody synthesis on immunoblot analysis. The presence of additional bands in CSF or of bands with a stronger intensity in CSF compared with serum was considered evidence of intrathecal synthesis. All 28 patients tested borderline or positive with C6 ELISA on serum. Of the 28 CSF samples, 17 (61%) and 19 (68%) tested positive with C6 ELISA using a threshold of 0.9 and 0.5 (optical density/cutoff). The C6 Lyme ELISA tested has a low sensitivity for antibody detection in cerebrospinal fluid compared with immunoblot analysis.
    MeSH term(s) Antibodies, Bacterial/cerebrospinal fluid ; Borrelia burgdorferi/immunology ; Cerebrospinal Fluid/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Lyme Neuroborreliosis/diagnosis ; Reagent Kits, Diagnostic ; Sensitivity and Specificity
    Chemical Substances Antibodies, Bacterial ; Reagent Kits, Diagnostic
    Language English
    Publishing date 2009-07
    Publishing country United States
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2009.03.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1845: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Evaluation of the V8 E-Class, a Novel Automated Capillary Isoelectric Focusing Instrument for Hemoglobinopathy Screening.

    Schiemsky, Toon / Brusselmans, Caroline / Nackers, Elke / Vermeersch, Pieter / Poesen, Koen / Desmet, Koen J O / Boeckx, Nancy / Kieffer, Davy M J

    American journal of clinical pathology

    2016  Volume 146, Issue 3, Page(s) 361–368

    Abstract: Objectives: We evaluated the performance of a novel capillary isoelectric focusing (CIEF) application for hemoglobinopathy screening on the recently introduced V8 E-Class platform.: Methods: Analytical performance of the V8 E-Class was evaluated and ... ...

    Abstract Objectives: We evaluated the performance of a novel capillary isoelectric focusing (CIEF) application for hemoglobinopathy screening on the recently introduced V8 E-Class platform.
    Methods: Analytical performance of the V8 E-Class was evaluated and included assessment of hemoglobin A2 (HbA2) imprecision; linearity for HbA2, fetal hemoglobin (HbF), and sickle hemoglobin (HbS); and carryover for HbS. Furthermore, a method comparison with the Minicap Flex Piercing (Sebia, Lisses, France), the Variant Classic (Bio-Rad Laboratories, Hercules, CA), and the G8 (Tosoh Europe, Amsterdam, the Netherlands) was done to assess analytical and clinical concordance.
    Results: Total HbA2 imprecision was 3.26% and 3.14% for normal and elevated HbA2 controls and 5.16% and 3.58% for a normal and a heterozygous HbS patient sample, respectively. HbA2, HbF, and HbS showed acceptable linearity, and no carryover was observed. The method comparison showed good analytical concordance (r > 0.95) except for a homozygous HbS subset (r = 0.532-0.704). A comparable phenomenon was seen for the clinical concordance with good agreement in samples without variants (weighted κ > 0.80) but poorer agreement in HbS samples (κ < 0.30).
    Conclusions: Good analytical performance was demonstrated for this novel CIEF application for hemoglobinopathy screening. Method comparison showed generally good correlation but highlights the need for standardization. Finally, software optimization could further add to its use for routine hemoglobinopathy screening.
    MeSH term(s) Electrophoresis, Capillary/instrumentation ; Electrophoresis, Capillary/methods ; Fetal Hemoglobin/analysis ; Hemoglobin A2/analysis ; Hemoglobin, Sickle/analysis ; Hemoglobinopathies/diagnosis ; Humans ; Isoelectric Focusing/instrumentation ; Isoelectric Focusing/methods ; Reproducibility of Results
    Chemical Substances Hemoglobin, Sickle ; Hemoglobin A2 (9034-53-1) ; Fetal Hemoglobin (9034-63-3)
    Language English
    Publishing date 2016-09
    Publishing country England
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 2944-0
    ISSN 1943-7722 ; 0002-9173
    ISSN (online) 1943-7722
    ISSN 0002-9173
    DOI 10.1093/ajcp/aqw124
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.91: Show issues Location:
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