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Article: Impact of the Resistance Responses to Stress Conditions Encountered in Food and Food Processing Environments on the Virulence and Growth Fitness of Non-Typhoidal

Guillén, Silvia / Nadal, Laura / Álvarez, Ignacio / Mañas, Pilar / Cebrián, Guillermo

2021 Volume 10, Issue 3

Abstract: The success ... ...

Abstract	The success of
Language	English
Publishing date	2021-03-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods10030617
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Article ; Online: Interfacial Fracture Energy Between New Translucent Zirconias and a Resin Cement.

Nadal, Laura Patricia Ortiz / Ramos, Nathália de Carvalho / Tribst, João Paulo Mendes / Anami, Lilian Costa / Melo, Renata Marques de / Bottino, Marco Antonio

The journal of adhesive dentistry

2022 Volume 24, Issue 1, Page(s) 147–154

Abstract: Purpose: To determine the interfacial fracture energy (IFE) and stress distribution of Brazil-nut-shaped specimens made of translucent zirconia and resin cement.: Materials and methods: Three types of translucent zirconia were used: 3Y-TZP (high, ... ...

Abstract	Purpose: To determine the interfacial fracture energy (IFE) and stress distribution of Brazil-nut-shaped specimens made of translucent zirconia and resin cement. Materials and methods: Three types of translucent zirconia were used: 3Y-TZP (high, Vita YZ HT), 4Y-TZP (super, Vita YZ ST), and 5Y-TZP (extra, Vita YZ XT). The adhesive surfaces were air abraded and 10-MDP-based resin cement was used. The cemented Brazil-nut-shaped specimens, with an elliptical defect in the center (as in real Brazil nuts), were thermally aged (5°C-55°C; 40,000 cycles). The IFE test was conducted with a piston to apply compression on the specimen, while the adhesive interface was positioned at four different angles (0, 10, 20, and 30 degrees) to measure the IFE during tensile, shear, and mixed failure modes. All adhesive interfaces were observed to determine failure patterns. The finite element analysis (FEA) was used to calculate tensile and shear stress distributions according to inclinations. Statistical analysis was conducted using the Kruskal-Wallis and Dunn's post-hoc tests (95%), as well as the Mann-Whitney test (95%) was applied to compare each group regarding the aging factor. Results: According to Kruskal-Wallis and Dunn's post-hoc tests, there were no statistically significant differences between non-aged (p > 0.05) and aged materials (p > 0.05). However, there was a significant difference between aged and non-aged materials for all inclinations (p < 0.05) (Mann-Whitney test). According to the FEA, the compressive loading of Brazil-nut-shaped specimens at different angles showed a predominance of tensile stress at 0 degrees and shear stress at 30 degrees. Conclusion: The IFE under predominantly shear stresses is higher than when specimens are subjected only to tensile stresses, which allows the interpretation that failures in the oral environmental will probably occur preferentially under tensile stresses, because less energy is needed. All translucent zirconia bonded to resin cement has similar IFE, and thermal aging negatively affects these bonding interfaces.
MeSH term(s)	Dental Bonding ; Dental Stress Analysis ; Materials Testing ; Resin Cements ; Surface Properties ; Zirconium
Chemical Substances	Resin Cements ; Zirconium (C6V6S92N3C)
Language	English
Publishing date	2022-04-13
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2002396-0
ISSN	1757-9988 ; 1461-5185
ISSN (online)	1757-9988
ISSN	1461-5185
DOI	10.3290/j.jad.b2916403
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Article ; Online: Muscarinic Receptors in Developmental Axonal Competition at the Neuromuscular Junction.

Tomàs, Josep / Lanuza, Maria A / Santafé, Manel M / Cilleros-Mañé, Víctor / Just-Borràs, Laia / Balanyà-Segura, Marta / Polishchuk, Aleksandra / Nadal, Laura / Tomàs, Marta / Garcia, Neus

Molecular neurobiology

2022 Volume 60, Issue 3, Page(s) 1580–1593

Abstract: In recent years, we have studied by immunohistochemistry, intracellular recording, and western blotting the role of the muscarinic acetylcholine receptors (mAChRs; ... ...

Abstract	In recent years, we have studied by immunohistochemistry, intracellular recording, and western blotting the role of the muscarinic acetylcholine receptors (mAChRs; M
MeSH term(s)	Animals ; Neuromuscular Junction/metabolism ; Axons/metabolism ; Receptors, Muscarinic/metabolism ; Acetylcholine/metabolism ; Calcium Channels/metabolism ; Mammals/metabolism
Chemical Substances	Receptors, Muscarinic ; Acetylcholine (N9YNS0M02X) ; Calcium Channels
Language	English
Publishing date	2022-12-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	645020-9
ISSN	1559-1182 ; 0893-7648
ISSN (online)	1559-1182
ISSN	0893-7648
DOI	10.1007/s12035-022-03154-1
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Article: Membrane Receptor-Induced Changes of the Protein Kinases A and C Activity May Play a Leading Role in Promoting Developmental Synapse Elimination at the Neuromuscular Junction.

Tomàs, Josep M / Garcia, Neus / Lanuza, Maria A / Nadal, Laura / Tomàs, Marta / Hurtado, Erica / Simó, Anna / Cilleros, Víctor

Frontiers in molecular neuroscience

2017 Volume 10, Page(s) 255

Abstract: Synapses that are overproduced during histogenesis in the nervous system are eventually lost and connectivity is refined. Membrane receptor signaling leads to activity-dependent mutual influence and competition between axons directly or with the ... ...

Abstract	Synapses that are overproduced during histogenesis in the nervous system are eventually lost and connectivity is refined. Membrane receptor signaling leads to activity-dependent mutual influence and competition between axons directly or with the involvement of the postsynaptic cell and the associated glial cell/s. Presynaptic muscarinic acetylcholine (ACh) receptors (subtypes mAChR; M
Language	English
Publishing date	2017-08-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2452967-9
ISSN	1662-5099
ISSN	1662-5099
DOI	10.3389/fnmol.2017.00255
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Article: Presynaptic Membrane Receptors Modulate ACh Release, Axonal Competition and Synapse Elimination during Neuromuscular Junction Development.

Tomàs, Josep / Garcia, Neus / Lanuza, Maria A / Santafé, Manel M / Tomàs, Marta / Nadal, Laura / Hurtado, Erica / Simó, Anna / Cilleros, Víctor

Frontiers in molecular neuroscience

2017 Volume 10, Page(s) 132

Abstract: During the histogenesis of the nervous system a lush production of neurons, which establish an excessive number of synapses, is followed by a drop in both neurons and synaptic contacts as maturation proceeds. Hebbian competition between axons with ... ...

Abstract	During the histogenesis of the nervous system a lush production of neurons, which establish an excessive number of synapses, is followed by a drop in both neurons and synaptic contacts as maturation proceeds. Hebbian competition between axons with different activities leads to the loss of roughly half of the neurons initially produced so connectivity is refined and specificity gained. The skeletal muscle fibers in the newborn neuromuscular junction (NMJ) are polyinnervated but by the end of the competition, 2 weeks later, the NMJ are innervated by only one axon. This peripheral synapse has long been used as a convenient model for synapse development. In the last few years, we have studied transmitter release and the local involvement of the presynaptic muscarinic acetylcholine autoreceptors (mAChR), adenosine autoreceptors (AR) and trophic factor receptors (TFR, for neurotrophins and trophic cytokines) during the development of NMJ and in the adult. This review article brings together previously published data and proposes a molecular background for developmental axonal competition and loss. At the end of the first week postnatal, these receptors modulate transmitter release in the various nerve terminals on polyinnervated NMJ and contribute to axonal competition and synapse elimination.
Language	English
Publishing date	2017-05-16
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2452967-9
ISSN	1662-5099
ISSN	1662-5099
DOI	10.3389/fnmol.2017.00132
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Article: Synaptic Activity and Muscle Contraction Increases PDK1 and PKCβI Phosphorylation in the Presynaptic Membrane of the Neuromuscular Junction.

Hurtado, Erica / Cilleros, Víctor / Just, Laia / Simó, Anna / Nadal, Laura / Tomàs, Marta / Garcia, Neus / Lanuza, Maria A / Tomàs, Josep

Frontiers in molecular neuroscience

2017 Volume 10, Page(s) 270

Abstract: Conventional protein kinase C βI (cPKCβI) is a conventional protein kinase C (PKC) isoform directly involved in the regulation of neurotransmitter release in the neuromuscular junction (NMJ). It is located exclusively at the nerve terminal and both ... ...

Abstract	Conventional protein kinase C βI (cPKCβI) is a conventional protein kinase C (PKC) isoform directly involved in the regulation of neurotransmitter release in the neuromuscular junction (NMJ). It is located exclusively at the nerve terminal and both synaptic activity and muscle contraction modulate its protein levels and phosphorylation. cPKCβI molecular maturation includes a series of phosphorylation steps, the first of which is mediated by phosphoinositide-dependent kinase 1 (PDK1). Here, we sought to localize PDK1 in the NMJ and investigate the hypothesis that synaptic activity and muscle contraction regulate in parallel PDK1 and cPKCβI phosphorylation in the membrane fraction. To differentiate the presynaptic and postsynaptic activities, we abolished muscle contraction with μ-conotoxin GIIIB (μ-CgTx-GIIIB) in some experiments before stimulation of the phrenic nerve (1 Hz, 30 min). Then, we analyzed total and membrane/cytosol fractions of skeletal muscle by Western blotting. Results showed that PDK1 is located exclusively in the nerve terminal of the NMJ. After nerve stimulation with and without coincident muscle contraction, total PDK1 and phosphorylated PDK1 (pPDK1) protein levels remained unaltered. However, synaptic activity specifically enhanced phosphorylation of PDK1 in the membrane, an important subcellular location for PDK1 function. This increase in pPDK1 coincides with a significant increase in the phosphorylation of its substrate cPKCβI also in the membrane fraction. Moreover, muscle contraction maintains PDK1 and pPDK1 but increases cPKCβI protein levels and its phosphorylation. Thus, even though PDK1 activity is maintained, pcPKCβI levels increase in concordance with total cPKCβI. Together, these results indicate that neuromuscular activity could induce the membrane targeting of pPDK1 in the nerve terminal of the NMJ to promote the phosphorylation of the cPKCβI, which is involved in ACh release.
Language	English
Publishing date	2017-08-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2452967-9
ISSN	1662-5099
ISSN	1662-5099
DOI	10.3389/fnmol.2017.00270
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Article: Evaluating Person-Centred Integrated Care to People with Complex Chronic Conditions: Early Implementation Results of the ProPCC Programme.

Mas, Miquel À / Miralles, Ramón / Ulldemolins, Maria J / Garcia, Ria / Gràcia, Sonia / Picaza, Josep M / Fernández, Mercedes Navarro / Rocabayera, Maria A / Rivera, Montserrat / Relaño, Núria / Asensio, Mireia Torres / Laporta, Pilar / Morcillo, Celia / Nadal, Laura / Hervás, Ramona / Fuguet, Dolors / Alba, Cristina / Banqué, Núria Miralles / Jimenez, Sònia /

Moreno, Miriam Moreno / Nogueras, Carmen / Navarro, Helena Manjón / López, Rosa / Hernández, Guillem / López-Seguí, Francesc / Ríos, Laura Ricou / Pons, Arnau / Prat, Nuria / Rey, Jordi Ara Del / Estrada, Oriol

International journal of integrated care

2023 Volume 23, Issue 4, Page(s) 18

Abstract: Introduction: The evaluation of integrated care programmes for : Methods: We analysed the intervention with retrospective data from May 2018 to December 2021 by describing the cohort complexity and by showing its 6-months pre-post impact on time ... ...

Abstract	Introduction: The evaluation of integrated care programmes for Methods: We analysed the intervention with retrospective data from May 2018 to December 2021 by describing the cohort complexity and by showing its 6-months pre-post impact on time spent at home and resources used: primary care visits, emergency department visits, hospital admissions and hospital stay. Findings: 264 cases were included (91% at home; 9% in nursing homes). 6-month pre vs. 6-months post results were (mean, p-value): primary care visits 8.2 vs. 11.5 (p < 0.05); emergency department visits 1.4 vs. 0.9 (p < 0.05); hospital admissions 0.7 vs. 0.5 (p < 0.05); hospital stay 12.8 vs. 7.9 days (p < 0.05). Time spent at home was 169.2 vs.174.2 days (p < 0.05). Conclusion: Early implementation of the ProPCC programme results in an increase in time spent at home (up to 3%) and significant reductions in emergency department attendance (-37.2%) and hospital stays (-38.3%). The increased use of primary care resources is compensated by the hospital resources savings, with a result in the average total cost of -46.3%.
Language	English
Publishing date	2023-12-13
Publishing country	England
Document type	Case Reports
ZDB-ID	2119289-3
ISSN	1568-4156
ISSN	1568-4156
DOI	10.5334/ijic.7585
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Article: Presynaptic Muscarinic Acetylcholine Receptors and TrkB Receptor Cooperate in the Elimination of Redundant Motor Nerve Terminals during Development.

Nadal, Laura / Garcia, Neus / Hurtado, Erica / Simó, Anna / Tomàs, Marta / Lanuza, Maria A / Cilleros, Victor / Tomàs, Josep

Frontiers in aging neuroscience

2017 Volume 9, Page(s) 24

Abstract: The development of the nervous system involves the overproduction of synapses but connectivity is refined by Hebbian activity-dependent axonal competition. The newborn skeletal muscle fibers are polyinnervated but, at the end of the competition process, ... ...

Abstract	The development of the nervous system involves the overproduction of synapses but connectivity is refined by Hebbian activity-dependent axonal competition. The newborn skeletal muscle fibers are polyinnervated but, at the end of the competition process, some days later, become innervated by a single axon. We used quantitative confocal imaging of the autofluorescent axons from transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice to investigate the possible cooperation of the muscarinic autoreceptors (mAChR, M
Language	English
Publishing date	2017-02-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2558898-9
ISSN	1663-4365
ISSN	1663-4365
DOI	10.3389/fnagi.2017.00024
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Article ; Online: nPKCε Mediates SNAP-25 Phosphorylation of Ser-187 in Basal Conditions and After Synaptic Activity at the Neuromuscular Junction.

Simó, Anna / Cilleros-Mañé, Victor / Just-Borràs, Laia / Hurtado, Erica / Nadal, Laura / Tomàs, Marta / Garcia, Neus / Lanuza, Maria A / Tomàs, Josep

Molecular neurobiology

2019 Volume 56, Issue 8, Page(s) 5346–5364

Abstract: Protein kinase C (PKC) and substrates like SNAP-25 regulate neurotransmission. At the neuromuscular junction (NMJ), PKC promotes neurotransmitter release during synaptic activity. Thirty minutes of muscle contraction enhances presynaptic PKC isoform ... ...

Abstract	Protein kinase C (PKC) and substrates like SNAP-25 regulate neurotransmission. At the neuromuscular junction (NMJ), PKC promotes neurotransmitter release during synaptic activity. Thirty minutes of muscle contraction enhances presynaptic PKC isoform levels, specifically cPKCβI and nPKCε, through retrograde BDNF/TrkB signaling. This establishes a larger pool of these PKC isoforms ready to promote neuromuscular transmission. The PKC phosphorylation site in SNAP-25 has been mapped to the serine 187 (Ser-187), which is known to enhance calcium-dependent neurotransmitter release in vitro. Here, we localize SNAP-25 at the NMJ and investigate whether cPKCβI and/or nPKCε regulate SNAP-25 phosphorylation. We also investigate whether nerve and muscle cell activities regulate differently SNAP-25 phosphorylation and the involvement of BDNF/TrkB signaling. Our results demonstrate that nPKCε isoform is essential to positively regulate SNAP-25 phosphorylation on Ser-187 and that muscle contraction prevents it. TrkB and cPKCβI do not regulate SNAP-25 protein level or its phosphorylation during neuromuscular activity. The results provide evidence that nerve terminals need both pre- and postsynaptic activities to modulate SNAP-25 phosphorylation and ensure an accurate neurotransmission process.
MeSH term(s)	Animals ; Brain-Derived Neurotrophic Factor/metabolism ; Models, Biological ; Muscle Contraction ; Muscle, Skeletal/metabolism ; Neuromuscular Junction/metabolism ; Phosphorylation ; Phosphoserine/metabolism ; Presynaptic Terminals/metabolism ; Protein Kinase C/metabolism ; Rats, Sprague-Dawley ; Receptor, trkB/metabolism ; Signal Transduction ; Synapses/metabolism ; Synaptosomal-Associated Protein 25/metabolism
Chemical Substances	Brain-Derived Neurotrophic Factor ; Synaptosomal-Associated Protein 25 ; Phosphoserine (17885-08-4) ; calcium-independent protein kinase C (EC 2.7.1.-) ; Receptor, trkB (EC 2.7.10.1) ; Protein Kinase C (EC 2.7.11.13)
Language	English
Publishing date	2019-01-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	645020-9
ISSN	1559-1182 ; 0893-7648
ISSN (online)	1559-1182
ISSN	0893-7648
DOI	10.1007/s12035-018-1462-5
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Article ; Online: Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction.

Nadal, Laura / Garcia, Neus / Hurtado, Erica / Simó, Anna / Tomàs, Marta / Lanuza, Maria A / Santafé, Manel / Tomàs, Josep

Molecular brain

2016 Volume 9, Issue 1, Page(s) 67

Abstract: Background: The development of the nervous system involves an initially exuberant production of neurons that make an excessive number of synaptic contacts. The initial overproduction of synapses promotes connectivity. Hebbian competition between axons ... ...

Abstract	Background: The development of the nervous system involves an initially exuberant production of neurons that make an excessive number of synaptic contacts. The initial overproduction of synapses promotes connectivity. Hebbian competition between axons with different activities (the least active are punished) leads to the loss of roughly half of the overproduced elements and this refines connectivity and increases specificity. The neuromuscular junction is innervated by a single axon at the end of the synapse elimination process and, because of its relative simplicity, has long been used as a model for studying the general principles of synapse development. The involvement of the presynaptic muscarinic ACh autoreceptors may allow for the direct competitive interaction between nerve endings through differential activity-dependent acetylcholine release in the synaptic cleft. Then, the most active ending may directly punish the less active ones. Our previous results indicate the existence in the weakest axons on the polyinnervated neonatal NMJ of an ACh release inhibition mechanism based on mAChR coupled to protein kinase C and voltage-dependent calcium channels. We suggest that this mechanism plays a role in the elimination of redundant neonatal synapses. Results: Here we used confocal microscopy and quantitative morphological analysis to count the number of brightly fluorescent axons per endplate in P7, P9 and P15 transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice. We investigate the involvement of individual mAChR M1-, M2- and M4-subtypes in the control of axonal elimination after the Levator auris longus muscle had been exposed to agonist and antagonist in vivo. We also analysed the role of adenosine receptor subtypes (A1 and A2A) and the tropomyosin-related kinase B receptor. The data show that postnatal axonal elimination is a regulated multireceptor mechanism that guaranteed the monoinnervation of the neuromuscular synapses. Conclusion: The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings, possibly helping to determine the winner or the lossers but, thereafter, the final elimination would occur with some autonomy and independently of postsynaptic maturation.
MeSH term(s)	Animals ; Animals, Newborn ; Atropine/pharmacology ; Axons/drug effects ; Axons/metabolism ; Female ; Fluorescent Antibody Technique ; Male ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Biological ; Neuromuscular Junction/drug effects ; Neuromuscular Junction/metabolism ; Oxotremorine/pharmacology ; Presynaptic Terminals/drug effects ; Presynaptic Terminals/metabolism ; Receptor, trkB/metabolism ; Receptors, Muscarinic/metabolism ; Receptors, Purinergic P1/metabolism ; Signal Transduction/drug effects
Chemical Substances	Receptors, Muscarinic ; Receptors, Purinergic P1 ; Oxotremorine (5RY0UWH1JL) ; Atropine (7C0697DR9I) ; Receptor, trkB (EC 2.7.10.1)
Language	English
Publishing date	2016--23
Publishing country	England
Document type	Journal Article
ZDB-ID	2436057-0
ISSN	1756-6606 ; 1756-6606
ISSN (online)	1756-6606
ISSN	1756-6606
DOI	10.1186/s13041-016-0248-9
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