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Artikel ; Online: Differential impact of ubiquitous and muscle dynamin 2 isoforms in muscle physiology and centronuclear myopathy

Raquel Gómez-Oca / Evelina Edelweiss / Sarah Djeddi / Mathias Gerbier / Xènia Massana-Muñoz / Mustapha Oulad-Abdelghani / Corinne Crucifix / Coralie Spiegelhalter / Nadia Messaddeq / Pierre Poussin-Courmontagne / Pascale Koebel / Belinda S. Cowling / Jocelyn Laporte

Nature Communications, Vol 13, Iss 1, Pp 1-

2022 Band 20

Abstract: Dynamin 2 is a large GTPase linked to several human diseases. Here, Gómez-Oca et al. investigate the functions of muscle dynamin 2 isoforms and provide insights into their differential implication in centronuclear myopathy pathogenesis and treatment. ...

Abstract	Dynamin 2 is a large GTPase linked to several human diseases. Here, Gómez-Oca et al. investigate the functions of muscle dynamin 2 isoforms and provide insights into their differential implication in centronuclear myopathy pathogenesis and treatment.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2022-11-01T00:00:00Z
Verlag	Nature Portfolio
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Cardiac forces regulate zebrafish heart valve delamination by modulating Nfat signaling.

Renee Wei-Yan Chow / Hajime Fukui / Wei Xuan Chan / Kok Soon Justin Tan / Stéphane Roth / Anne-Laure Duchemin / Nadia Messaddeq / Hiroyuki Nakajima / Fei Liu / Nathalie Faggianelli-Conrozier / Andrey S Klymchenko / Yap Choon Hwai / Naoki Mochizuki / Julien Vermot

PLoS Biology, Vol 20, Iss 1, p e

2022 Band 3001505

Abstract: In the clinic, most cases of congenital heart valve defects are thought to arise through errors that occur after the endothelial-mesenchymal transition (EndoMT) stage of valve development. Although mechanical forces caused by heartbeat are essential ... ...

Abstract	In the clinic, most cases of congenital heart valve defects are thought to arise through errors that occur after the endothelial-mesenchymal transition (EndoMT) stage of valve development. Although mechanical forces caused by heartbeat are essential modulators of cardiovascular development, their role in these later developmental events is poorly understood. To address this question, we used the zebrafish superior atrioventricular valve (AV) as a model. We found that cellularized cushions of the superior atrioventricular canal (AVC) morph into valve leaflets via mesenchymal-endothelial transition (MEndoT) and tissue sheet delamination. Defects in delamination result in thickened, hyperplastic valves, and reduced heart function. Mechanical, chemical, and genetic perturbation of cardiac forces showed that mechanical stimuli are important regulators of valve delamination. Mechanistically, we show that forces modulate Nfatc activity to control delamination. Together, our results establish the cellular and molecular signature of cardiac valve delamination in vivo and demonstrate the continuous regulatory role of mechanical forces and blood flow during valve formation.
Schlagwörter	Biology (General) ; QH301-705.5
Thema/Rubrik (Code)	621
Sprache	Englisch
Erscheinungsdatum	2022-01-01T00:00:00Z
Verlag	Public Library of Science (PLoS)
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection

Marion Ferren / Valérie Favède / Didier Decimo / Mathieu Iampietro / Nicole A. P. Lieberman / Jean-Luc Weickert / Rodolphe Pelissier / Magalie Mazelier / Olivier Terrier / Anne Moscona / Matteo Porotto / Alexander L. Greninger / Nadia Messaddeq / Branka Horvat / Cyrille Mathieu

Nature Communications, Vol 12, Iss 1, Pp 1-

2021 Band 17

Abstract: Here, Ferren et al. isolate Syrian hamster brainstem and lung tissue to establish ex vivo culture systems to study SARS-CoV-2 local viral tropism, immune response and tissue pathology. Further, they provide evidence that these systems can be used for ... ...

Abstract	Here, Ferren et al. isolate Syrian hamster brainstem and lung tissue to establish ex vivo culture systems to study SARS-CoV-2 local viral tropism, immune response and tissue pathology. Further, they provide evidence that these systems can be used for screening of anti-viral compounds.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2021-10-01T00:00:00Z
Verlag	Nature Portfolio
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Modulation of large dense core vesicle insulin content mediates rhythmic hormone release from pancreatic beta cells over the 24h cycle.

Aurore Quinault / Corinne Leloup / Geoffrey Denwood / Coralie Spiegelhalter / Marianne Rodriguez / Philippe Lefebvre / Nadia Messaddeq / Quan Zhang / Catherine Dacquet / Luc Pénicaud / Stephan C Collins

PLoS ONE, Vol 13, Iss 3, p e

2018 Band 0193882

Abstract: The rhythmic nature of insulin secretion over the 24h cycle in pancreatic islets has been mostly investigated using transcriptomics studies showing that modulation of insulin secretion over this cycle is achieved via distal stages of insulin secretion. ... ...

Abstract	The rhythmic nature of insulin secretion over the 24h cycle in pancreatic islets has been mostly investigated using transcriptomics studies showing that modulation of insulin secretion over this cycle is achieved via distal stages of insulin secretion. We set out to measure β-cell exocytosis using in depth cell physiology techniques at several time points. In agreement with the activity and feeding pattern of nocturnal rodents, we find that C57/Bl6J islets in culture for 24h exhibit higher insulin secretion during the corresponding dark phase than in the light phase (Zeitgeber Time ZT20 and ZT8, respectively, in vivo). Glucose-induced insulin secretion is increased by 21% despite normal intracellular Ca2+ transients and depolarization-evoked exocytosis, as measured by whole-cell capacitance measurements. This paradox is explained by a 1.37-fold increase in beta cell insulin content. Ultramorphological analyses show that vesicle size and density are unaltered, demonstrating that intravesicular insulin content per granule is modulated over the 24h cycle. Proinsulin levels did not change between ZT8 and ZT20. Islet glucagon content was inversely proportional to insulin content indicating that this unique feature is likely to support a physiological role. Microarray data identified the differential expression of 301 transcripts, of which 26 are miRNAs and 54 are known genes (including C2cd4b, a gene previously involved in insulin processing, and clock genes such as Bmal1 and Rev-erbα). Mouse β-cell secretion over the full course of the 24h cycle may rely on several distinct cellular functions but late night increase in insulin secretion depends solely on granule insulin content.
Schlagwörter	Medicine ; R ; Science ; Q
Thema/Rubrik (Code)	571
Sprache	Englisch
Erscheinungsdatum	2018-01-01T00:00:00Z
Verlag	Public Library of Science (PLoS)
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: ISCA1 is essential for mitochondrial Fe4S4 biogenesis in vivo

Lena Kristina Beilschmidt / Sandrine Ollagnier de Choudens / Marjorie Fournier / Ioannis Sanakis / Marc-André Hograindleur / Martin Clémancey / Geneviève Blondin / Stéphane Schmucker / Aurélie Eisenmann / Amélie Weiss / Pascale Koebel / Nadia Messaddeq / Hélène Puccio / Alain Martelli

Nature Communications, Vol 8, Iss 1, Pp 1-

2017 Band 12

Abstract: The mitochondrial proteins ISCA1 and ISCA2 form a complex that is involved in the biogenesis of Fe–S clusters. Here the authors report that ISCA1 and ISCA2 interact differently with proteins of the Fe–S machinery and that under certain conditions, ISCA2 ... ...

Abstract	The mitochondrial proteins ISCA1 and ISCA2 form a complex that is involved in the biogenesis of Fe–S clusters. Here the authors report that ISCA1 and ISCA2 interact differently with proteins of the Fe–S machinery and that under certain conditions, ISCA2 seems dispensable for Fe–S biogenesis.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2017-05-01T00:00:00Z
Verlag	Nature Portfolio
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Antisense oligonucleotide-mediated Dnm2 knockdown prevents and reverts myotubular myopathy in mice

Hichem Tasfaout / Suzie Buono / Shuling Guo / Christine Kretz / Nadia Messaddeq / Sheri Booten / Sarah Greenlee / Brett P. Monia / Belinda S. Cowling / Jocelyn Laporte

Nature Communications, Vol 8, Iss 1, Pp 1-

2017 Band 13

Abstract: X-linked myotubular myopathy is caused by mutations in the gene coding for myotubularin 1, and is characterized by overexpression of dynamin 2. Here the authors develop antisense oligonucleotides to dynamin 2, and show that systemic injection leads to ... ...

Abstract	X-linked myotubular myopathy is caused by mutations in the gene coding for myotubularin 1, and is characterized by overexpression of dynamin 2. Here the authors develop antisense oligonucleotides to dynamin 2, and show that systemic injection leads to improved pathology in mice.
Schlagwörter	Science ; Q
Sprache	Englisch
Erscheinungsdatum	2017-06-01T00:00:00Z
Verlag	Nature Portfolio
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel: Well-organized spheroids as a new platform to examine cell interaction and behaviour during organ development

BÃ©cavin, Thibault / Sabine Kuchler-Bopp / Tunay KÃ¶kten / Olivier Huck / Nadia Messaddeq / HervÃ© Lesot / Etienne Deveaux / Nadia Benkirane-Jessel / Keller Laetitia

Cell and tissue research. 2016 Dec., v. 366, no. 3

2016

Abstract: We present an experimental method allowing the production of three-dimensional organ-like structures, namely microtissues (MTs), in vitro without the need for exogenous extracellular matrix (ECM) or growth factors. Submandibular salivary glands ( ... ...

Abstract	We present an experimental method allowing the production of three-dimensional organ-like structures, namely microtissues (MTs), in vitro without the need for exogenous extracellular matrix (ECM) or growth factors. Submandibular salivary glands (embryonic day ED14), kidneys (ED13) and lungs (ED13) were harvested from mouse embryos and dissociated into single cells by enzyme treatment. Single cells were seeded into special hanging drop culture plates (InSphero) and cultured for up to 14Â days to obtain MTs. This strategy permitted full control of the quantity of seeded cells. The development of the MTs into organs was followed histologically and immunohistochemically. Well-organized epithelial structures surrounded by a basal lamina were formed, as confirmed by transmission electron microscopy. Expression of E-cadherin, vimentin, fibronectin and Î±-SMA was compared in organs and corresponding MTs by real-time quantitative polymerase chain reaction. Branching morphogenesis was induced in MTs (as shown by histology and immunostaining for fibronectin and perlecan) and was conserved even after 14Â days of culture. MTs continued their development and their epithelial structures were comparable with those of the physiological organ at postnatal day 2 (PN2). Expression of aquaporins was investigated to obtain better support for the functional differentiation of epithelial cells. Histogenesis proceeded and led to the start of organogenesis. This experimental model might improve our knowledge of epithelial-mesenchymal histogenesis and can be employed to study development or cellular organization during the embryonic formation of organs.
Schlagwörter	aquaporins ; cadherins ; enzymatic treatment ; epithelial cells ; epithelium ; extracellular matrix ; fibronectins ; growth factors ; immunohistochemistry ; kidneys ; lungs ; mice ; models ; organogenesis ; salivary glands ; transmission electron microscopy ; vimentin
Sprache	Englisch
Erscheinungsverlauf	2016-12
Umfang	p. 601-615.
Erscheinungsort	Springer Berlin Heidelberg
Dokumenttyp	Artikel
ZDB-ID	125067-x
ISSN	1432-0878 ; 0302-766X
ISSN (online)	1432-0878
ISSN	0302-766X
DOI	10.1007/s00441-016-2487-6
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: Magnetite- and Iodine-Containing Nanoemulsion as a Dual Modal Contrast Agent for X-ray/Magnetic Resonance Imaging

Wallyn, Justine / Nicolas Anton / Damien Mertz / Sylvie Begin-Colin / Francis Perton / Christophe A. Serra / Florence Franconi / Laurent Lemaire / Manuela Chiper / Hélène Libouban / Nadia Messaddeq / Halina Anton / Thierry F. Vandamme

ACS applied materials & interfaces. 2018 Dec. 13, v. 11, no. 1

2018

Abstract: Noninvasive diagnostic by imaging combined with a contrast agent (CA) is by now the most used technique to get insight into human bodies. X-ray and magnetic resonance imaging (MRI) are widely used technologies providing complementary results. Nowadays, ... ...

Abstract	Noninvasive diagnostic by imaging combined with a contrast agent (CA) is by now the most used technique to get insight into human bodies. X-ray and magnetic resonance imaging (MRI) are widely used technologies providing complementary results. Nowadays, it seems clear that bimodal CAs could be an emerging approach to increase the patient compliance, accessing different imaging modalities with a single CA injection. Owing to versatile designs, targeting properties, and high payload capacity, nanocarriers are considered as a viable solution to reach this goal. In this study, we investigated efficient superparamagnetic iron oxide nanoparticle (SPION)-loaded iodinated nano-emulsions (NEs) as dual modal injectable CAs for X-ray imaging and MRI. The strength of this new CA lies not only in its dual modal contrasting properties and biocompatibility, but also in the simplicity of the nanoparticulate assembling: iodinated oily core was synthesized by the triiodo-benzene group grafting on vitamin E (41.7% of iodine) via esterification, and SPIONs were produced by thermal decomposition during 2, 4, and 6 h to generate SPIONs with different morphologies and magnetic properties. SPIONs with most anisotropic shape and characterized by the highest r₂/r₁ ratio once encapsulated into iodinated NE were used for animal experimentation. The in vivo investigation showed an excellent contrast modification because of the presence of the selected NEs, for both imaging techniques explored, that is, MRI and X-ray imaging. This work provides the description and in vivo application of a simple and efficient nanoparticulate system capable of enhancing contrast for both preclinical imaging modalities, MRI, and computed tomography.
Schlagwörter	X-radiation ; animal experimentation ; anisotropy ; biocompatibility ; computed tomography ; esterification ; image analysis ; iodine ; iron oxides ; magnetic properties ; magnetic resonance imaging ; nanocarriers ; nanoemulsions ; nanoparticles ; patient compliance ; thermal degradation ; vitamin E
Sprache	Englisch
Erscheinungsverlauf	2018-1213
Umfang	p. 403-416.
Erscheinungsort	American Chemical Society
Dokumenttyp	Artikel
ISSN	1944-8252
DOI	10.1021/acsami.8b19517
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: Microfluidic-Assisted Production of Size-Controlled Superparamagnetic Iron Oxide Nanoparticles-Loaded Poly(methyl methacrylate) Nanohybrids

Ding, Shukai / Alexandre Collard / Chiara Taddei / Christophe A. Serra / Justine Wallyn / Marc Schmutz / Meriem Er-Rafik / Michele Giordano / Mohamad Kassem / Mohamed F. Attia / Nadia Messaddeq / Nicolas Anton / Thierry F. Vandamme / Wei Yu

Langmuir. 2018 Feb. 06, v. 34, no. 5

2018

Abstract: In this paper, superparamagnetic iron oxide nanoparticles (SPIONs, around 6 nm) encapsulated in poly(methyl methacrylate) nanoparticles (PMMA NPs) with controlled sizes ranging from 100 to 200 nm have been successfully produced. The hybrid polymeric NPs ... ...

Abstract	In this paper, superparamagnetic iron oxide nanoparticles (SPIONs, around 6 nm) encapsulated in poly(methyl methacrylate) nanoparticles (PMMA NPs) with controlled sizes ranging from 100 to 200 nm have been successfully produced. The hybrid polymeric NPs were prepared following two different methods: (1) nanoprecipitation and (2) nanoemulsification–evaporation. These two methods were implemented in two different microprocesses based on the use of an impact jet micromixer and an elongational-flow microemulsifier. SPIONs-loaded PMMA NPs synthesized by the two methods presented completely different physicochemical properties. The polymeric NPs prepared with the micromixer-assisted nanoprecipitation method showed a heterogeneous dispersion of SPIONs inside the polymer matrix, an encapsulation efficiency close to 100 wt %, and an irregular shape. In contrast, the polymeric NPs prepared with the microfluidic-assisted nanoemulsification–evaporation method showed a homogeneous dispersion, an almost complete encapsulation, and a spherical shape. The properties of the polymeric NPs have been characterized by dynamic light scattering, thermogravimetric analysis, and transmission electron microscope. In vitro cytotoxicity assays were also performed on the nanohybrids and pure PMMA NPs.
Schlagwörter	encapsulation ; iron oxides ; light scattering ; nanohybrids ; nanoparticles ; physicochemical properties ; polymethylmethacrylate ; thermogravimetry ; toxicity testing ; transmission electron microscopes
Sprache	Englisch
Erscheinungsverlauf	2018-0206
Umfang	p. 1981-1991.
Erscheinungsort	American Chemical Society
Dokumenttyp	Artikel
ZDB-ID	2005937-1
ISSN	1520-5827 ; 0743-7463
ISSN (online)	1520-5827
ISSN	0743-7463
DOI	10.1021/acs.langmuir.7b01928
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: Prokineticin receptor 1 as a novel suppressor of preadipocyte proliferation and differentiation to control obesity.

Cécilia Szatkowski / Judith Vallet / Mojdeh Dormishian / Nadia Messaddeq / Phillippe Valet / Mounia Boulberdaa / Daniel Metzger / Pierre Chambon / Canan G Nebigil

PLoS ONE, Vol 8, Iss 12, p e

2013 Band 81175

Abstract: BACKGROUND: Adipocyte renewal from preadipocytes occurs throughout the lifetime and contributes to obesity. To date, little is known about the mechanisms that control preadipocyte proliferation and differentiation. Prokineticin-2 is an angiogenic and ... ...

Abstract	BACKGROUND: Adipocyte renewal from preadipocytes occurs throughout the lifetime and contributes to obesity. To date, little is known about the mechanisms that control preadipocyte proliferation and differentiation. Prokineticin-2 is an angiogenic and anorexigenic hormone that activate two G protein-coupled receptors (GPCRs): PKR1 and PKR2. Prokineticin-2 regulates food intake and energy metabolism via central mechanisms (PKR2). The peripheral effect of prokineticin-2 on adipocytes/preadipocytes has not been studied yet. METHODOLOGY/PRINCIPAL FINDINGS: Since adipocytes and preadipocytes express mainly prokineticin receptor-1 (PKR1), here, we explored the role of PKR1 in adipose tissue expansion, generating PKR1-null (PKR1(-/-)) and adipocyte-specific (PKR1(ad-/-)) mutant mice, and using murine and human preadipocyte cell lines. Both PKR1(-/-) and PKR1(ad-/-) had excessive abdominal adipose tissue, but only PKR1(-/-) mice showed severe obesity and diabetes-like syndrome. PKR1(ad-/-)) mice had increased proliferating preadipocytes and newly formed adipocyte levels, leading to expansion of adipose tissue. Using PKR1-knockdown in 3T3-L1 preadipocytes, we show that PKR1 directly inhibits preadipocyte proliferation and differentiation. These PKR1 cell autonomous actions appear targeted at preadipocyte cell cycle regulatory pathways, through reducing cyclin D, E, cdk2, c-Myc levels. CONCLUSIONS/SIGNIFICANCE: These results suggest PKR1 to be a crucial player in the preadipocyte proliferation and differentiation. Our data should facilitate studies of both the pathogenesis and therapy of obesity in humans.
Schlagwörter	Medicine ; R ; Science ; Q
Thema/Rubrik (Code)	616
Sprache	Englisch
Erscheinungsdatum	2013-01-01T00:00:00Z
Verlag	Public Library of Science (PLoS)
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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