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Article ; Online: Editorial: Inflammasomes in infectious diseases, cell death and ROS generation: stimulation by microorganisms and membrane-derived microvesicles.

Nagao, Prescilla Emy / Santos, Louisy / Keestra-Gounder, A Marijke

2024 Volume 15, Page(s) 1378506

MeSH term(s)	Humans ; Inflammasomes/metabolism ; Reactive Oxygen Species/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Cell Death ; Communicable Diseases
Chemical Substances	Inflammasomes ; Reactive Oxygen Species ; NLR Family, Pyrin Domain-Containing 3 Protein
Language	English
Publishing date	2024-02-27
Publishing country	Switzerland
Document type	Editorial ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1378506
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Article ; Online: Inflammasome activation by Gram-positive bacteria: Mechanisms of activation and regulation.

Keestra-Gounder, A Marijke / Nagao, Prescilla Emy

Frontiers in immunology

2023 Volume 14, Page(s) 1075834

Abstract: The inflammasomes are intracellular multimeric protein complexes consisting of an innate immune sensor, the adapter protein ASC and the inflammatory caspases-1 and/or -11 and are important for the host defense against pathogens. Activaton of the receptor ...

Abstract	The inflammasomes are intracellular multimeric protein complexes consisting of an innate immune sensor, the adapter protein ASC and the inflammatory caspases-1 and/or -11 and are important for the host defense against pathogens. Activaton of the receptor leads to formation of the inflammasomes and subsequent processing and activation of caspase-1 that cleaves the proinflammatory cytokines IL-1β and IL-18. Active caspase-1, and in some instances caspase-11, cleaves gasdermin D that translocates to the cell membrane where it forms pores resulting in the cell death program called pyroptosis. Inflammasomes can detect a range of microbial ligands through direct interaction or indirectly through diverse cellular processes including changes in ion fluxes, production of reactive oxygen species and disruption of various host cell functions. In this review, we will focus on the NLRP3, NLRP6, NLRC4 and AIM2 inflammasomes and how they are activated and regulated during infections with Gram-positive bacteria, including
MeSH term(s)	Inflammasomes/metabolism ; Carrier Proteins/metabolism ; Caspases/metabolism ; Cytokines/metabolism ; Caspase 1/metabolism ; Gram-Positive Bacteria/metabolism
Chemical Substances	Inflammasomes ; Carrier Proteins ; Caspases (EC 3.4.22.-) ; Cytokines ; Caspase 1 (EC 3.4.22.36)
Language	English
Publishing date	2023-01-24
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1075834
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Article: Editorial:

Nagao, Prescilla Emy / Burkovski, Andreas / Mattos-Guaraldi, Ana Luíza

Frontiers in microbiology

2022 Volume 13, Page(s) 867210

Language	English
Publishing date	2022-02-28
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2022.867210
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Article ; Online: Role of Caveolin-1 in Sepsis - A Mini-Review.

Lannes-Costa, Pamella Silva / Pimentel, Bruna Alves da Silva / Nagao, Prescilla Emy

Frontiers in immunology

2022 Volume 13, Page(s) 902907

Abstract: Sepsis is a generalized disease characterized by an extreme response to a severe infection. Moreover, challenges remain in the diagnosis, treatment and management of septic patients. In this mini-review we demonstrate developments on cellular ... ...

Abstract	Sepsis is a generalized disease characterized by an extreme response to a severe infection. Moreover, challenges remain in the diagnosis, treatment and management of septic patients. In this mini-review we demonstrate developments on cellular pathogenesis and the role of Caveolin-1 (Cav-1) in sepsis. Studies have shown that Cav-1 has a significant role in sepsis through the regulation of membrane traffic and intracellular signaling pathways. In addition, activation of apoptosis/autophagy is considered relevant for the progression and development of sepsis. However, how Cav-1 is involved in sepsis remains unclear, and the precise mechanisms need to be further investigated. Finally, the role of Cav-1 in altering cell permeability during inflammation, in sepsis caused by microorganisms, apoptosis/autophagy activation and new therapies under study are discussed in this mini-review.
MeSH term(s)	Autophagy/physiology ; Caveolin 1/genetics ; Caveolin 1/metabolism ; Humans ; Permeability ; Sepsis/genetics ; Sepsis/metabolism ; Signal Transduction
Chemical Substances	Caveolin 1
Language	English
Publishing date	2022-07-15
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.902907
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Article ; Online: Streptococcus agalactiae strains isolated from cancer patients in Rio de Janeiro, Brazil.

de Figueiredo Sanches, Glenda / Lannes-Costa, Pamella Silva / Cristoforêto, Melissa Coimbra / Doran, Kelly S / Mattos-Guaraldi, Ana Luíza / Nagao, Prescilla Emy

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

2021 Volume 52, Issue 1, Page(s) 303–310

Abstract: Streptococcus agalactiae is a recognized pathogen associated with infections in neonates, elderly, and immunocompromised adults, particularly those with cancer. In the present investigation, clinical-epidemiological features, multidrug resistance ... ...

Abstract	Streptococcus agalactiae is a recognized pathogen associated with infections in neonates, elderly, and immunocompromised adults, particularly those with cancer. In the present investigation, clinical-epidemiological features, multidrug resistance profiles, and virulence genes of S. agalactiae strains isolated from cancer patients were investigated. S. agalactiae capsular distribution assays demonstrated that Ia (43.6%) and V (23.6%) types were predominantly detected among 55 clinical isolates tested; only one strain (GBS1428) was capsular type III/ST-17. The fbsB and hylB genes were detected in all isolates, while the iag, lmb, and fbsA genes were detected in 94.5%, 91%, and 91% of oncological isolates, respectively. The combination of PI-1 and PI-2a was the most common (60%) among S. agalactiae strains isolated from oncologic patients. S. agalactiae strains were resistant to tetracycline (85.5%), erythromycin (9%), and clindamycin (5.5%). Norfloxacin non-susceptible was detected in 7.3% of S. agalactiae strains. Our findings reinforce the need for S. agalactiae control measures in Brazil, including cancer patients.
MeSH term(s)	Adolescent ; Adult ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Brazil/epidemiology ; Drug Resistance, Multiple, Bacterial ; Genotype ; Humans ; Microbial Sensitivity Tests ; Middle Aged ; Neoplasms/complications ; Neoplasms/microbiology ; Streptococcal Infections/epidemiology ; Streptococcal Infections/microbiology ; Streptococcus agalactiae/drug effects ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/isolation & purification ; Streptococcus agalactiae/pathogenicity ; Virulence Factors/genetics ; Young Adult
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Virulence Factors
Language	English
Publishing date	2021-01-04
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2017175-4
ISSN	1678-4405 ; 1517-8382
ISSN (online)	1678-4405
ISSN	1517-8382
DOI	10.1007/s42770-020-00419-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

2023 Volume 12, Issue 4

Abstract: Streptococcus ... ...

Abstract	Streptococcus agalactiae
Language	English
Publishing date	2023-04-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12040580
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Article ; Online: Involvement of lipid microdomains in human endothelial cells infected by Streptococcus agalactiae type III belonging to the hypervirulent ST-17.

Ferreira, Beatriz Jandre / Lannes-Costa, Pamella Silva / Santos, Gabriela da Silva / Mermelstein, Cláudia / Einicker-Lamas, Marcelo / Nagao, Prescilla Emy

Memorias do Instituto Oswaldo Cruz

2020 Volume 115, Page(s) e190398

Abstract: Background: Streptococcus agalactiae capsular type III strains are a leading cause of invasive neonatal infections. Many pathogens have developed mechanisms to escape from host defense response using the host membrane microdomain machinery. Lipid rafts ... ...

Abstract	Background: Streptococcus agalactiae capsular type III strains are a leading cause of invasive neonatal infections. Many pathogens have developed mechanisms to escape from host defense response using the host membrane microdomain machinery. Lipid rafts play an important role in a variety of cellular functions and the benefit provided by interaction with lipid rafts can vary from one pathogen to another. Objectives: This study aims to evaluate the involvement of membrane microdomains during infection of human endothelial cell by S. agalactiae. Methods: The effects of cholesterol depletion and PI3K/AKT signaling pathway activation during S. agalactiae-human umbilical vein endothelial cells (HUVEC) interaction were analysed by pre-treatment with methyl-β-cyclodextrin (MβCD) or LY294002 inhibitors, immunofluorescence and immunoblot analysis. The involvement of lipid rafts was analysed by colocalisation of bacteria with flotillin-1 and caveolin-1 using fluorescence confocal microscopy. Findings: In this work, we demonstrated the importance of the integrity of lipid rafts microdomains and activation of PI3K/Akt pathway during invasion of S. agalactiae strain to HUVEC cells. Our results suggest the involvement of flotillin-1 and caveolin-1 during the invasion of S. agalactiae strain in HUVEC cells. Conclusions: The collection of our results suggests that lipid microdomain affects the interaction of S. agalactiae type III belonging to the hypervirulent ST-17 with HUVEC cells through PI3K/Akt signaling pathway.
MeSH term(s)	Endothelial Cells/virology ; Humans ; Infant, Newborn ; Membrane Lipids ; Membrane Microdomains/virology ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/pathogenicity ; Virulence
Chemical Substances	Membrane Lipids
Keywords	covid19
Language	English
Publishing date	2020-03-16
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	953293-6
ISSN	1678-8060 ; 0074-0276
ISSN (online)	1678-8060
ISSN	0074-0276
DOI	10.1590/0074-02760190398
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Article ; Online: Resistance to Antiseptics and Disinfectants of Planktonic and Biofilm-Associated Forms of

Souza, Cassius de / Mota, Higor Franceschi / Faria, Yuri Vieira / Cabral, Fellipe de Oliveira / Oliveira, Dryelle Rodrigues de / Sant'Anna, Lincoln de Oliveira / Nagao, Prescilla Emy / Santos, Cintia da Silva / Moreira, Lílian Oliveira / Mattos-Guaraldi, Ana Luíza

Microbial drug resistance (Larchmont, N.Y.)

2020 Volume 26, Issue 12, Page(s) 1546–1558

Abstract: Disinfection and antisepsis are of primary importance in controlling nosocomial infections and outbreaks by pathogens expressing multiple resistance to antimicrobial agents (multidrug-resistant [MDR]) used in therapy. Nowadays, infections related to ... ...

Abstract	Disinfection and antisepsis are of primary importance in controlling nosocomial infections and outbreaks by pathogens expressing multiple resistance to antimicrobial agents (multidrug-resistant [MDR]) used in therapy. Nowadays, infections related to health services (HAIs) due to MDR and multidrug-susceptible (MDS)
MeSH term(s)	Adult ; Anti-Infective Agents, Local/pharmacology ; Biofilms/drug effects ; Corynebacterium/drug effects ; Cross Infection/prevention & control ; Disinfectants/pharmacology ; Drug Resistance, Multiple, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Female ; Humans ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Plankton/drug effects ; Virulence
Chemical Substances	Anti-Infective Agents, Local ; Disinfectants
Language	English
Publishing date	2020-05-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	1290490-9
ISSN	1931-8448 ; 1076-6294
ISSN (online)	1931-8448
ISSN	1076-6294
DOI	10.1089/mdr.2019.0124
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Zs.A 4435: Show issues

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Article ; Online: Comparative genomic analysis and identification of pathogenicity islands of hypervirulent ST-17 Streptococcus agalactiae Brazilian strain.

Lannes-Costa, Pamella Silva / Baraúna, Rafael Azevedo / Ramos, Juliana Nunes / Veras, João Flavio Carneiro / Conceição, Marcos Vinícius Reis / Vieira, Verônica Viana / de Mattos-Guaraldi, Ana Luiza / Ramos, Rommel Thiago Juca / Doran, Kelly S / Silva, Artur / Nagao, Prescilla Emy

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

2020 Volume 80, Page(s) 104195

Abstract: Streptococcus agalactiae are important pathogenic bacteria that cause severe infections in humans, especially neonates. The mechanism by which ST-17 causes invasive infections than other STs is not well understood. In this study, we sequenced the first ... ...

Abstract	Streptococcus agalactiae are important pathogenic bacteria that cause severe infections in humans, especially neonates. The mechanism by which ST-17 causes invasive infections than other STs is not well understood. In this study, we sequenced the first genome of a S. agalactiae ST-17 strain isolated in Brazil using the Illumina HiSeq 2500 technology. S. agalactiae GBS90356 ST-17 belongs to the capsular type III and was isolated from a neonatal with a fatal case of meningitis. The genome presented a size of 2.03 Mbp and a G + C content of 35.2%. S. agalactiae has 706 genes in its core genome and an open pan-genome with a size of 5.020 genes, suggesting a high genomic plasticity. GIPSy software was used to identify 10 Pathogenicity islands (PAIs) which corresponded to 15% of the genome size. IslandViewer4 corroborated the prediction of six PAIs. The pathogenicity islands showed important virulence factors genes for S. agalactiae e.g. neu, cps, dlt, fbs, cfb, lmb. SignalP detected 20 proteins with signal peptides among the 352 proteins found in PAIs, which 60% were located in the SagPAI_5. SagPAI_2 and 5 were mainly detected in ST-17 strains studied. Moreover, we identified 51 unique genes, 9 recombination regions and a large number of SNPs with an average of 760.3 polymorphisms, which can be related with high genomic plasticity and virulence during host-pathogen interactions. Our results showed implications for pathogenesis, evolution, concept of species and in silico analysis value to understand the epidemiology and genome plasticity of S. agalactiae.
MeSH term(s)	Brazil/epidemiology ; Computational Biology/methods ; Genome, Bacterial ; Genomics/methods ; Humans ; Molecular Sequence Annotation ; Phylogeny ; Public Health Surveillance ; Streptococcal Infections/epidemiology ; Streptococcal Infections/microbiology ; Streptococcus agalactiae/classification ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/isolation & purification ; Streptococcus agalactiae/pathogenicity ; Virulence/genetics ; Virulence Factors/genetics
Chemical Substances	Virulence Factors
Language	English
Publishing date	2020-01-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2037068-4
ISSN	1567-7257 ; 1567-1348
ISSN (online)	1567-7257
ISSN	1567-1348
DOI	10.1016/j.meegid.2020.104195
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Zs.A 5645: Show issues

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Article ; Online: Reactive oxygen species generation mediated by NADPH oxidase and PI3K/Akt pathways contribute to invasion of Streptococcus agalactiae in human endothelial cells.

Oliveira, Jessica Silva Santos de / Santos, Gabriela da Silva / Moraes, João Alfredo / Saliba, Alessandra Mattos / Barja-Fidalgo, Thereza Christina / Mattos-Guaraldi, Ana Luíza / Nagao, Prescilla Emy

Memorias do Instituto Oswaldo Cruz

2018 Volume 113, Issue 6, Page(s) e140421

Abstract: BACKGROUND Streptococcus agalactiae can causes sepsis, pneumonia, and meningitis in neonates, the elderly, and immunocompromised patients. Although the virulence properties of S. agalactiae have been partially elucidated, the molecular mechanisms related ...

Abstract	BACKGROUND Streptococcus agalactiae can causes sepsis, pneumonia, and meningitis in neonates, the elderly, and immunocompromised patients. Although the virulence properties of S. agalactiae have been partially elucidated, the molecular mechanisms related to reactive oxygen species (ROS) generation in infected human endothelial cells need further investigation. OBJECTIVES This study aimed to evaluate the influence of oxidative stress in human umbilical vein endothelial cells (HUVECs) during S. agalactiae infection. METHODS ROS production during S. agalactiae-HUVEC infection was detected using the probe CM-H2DCFDA. Microfilaments labelled with phalloidin-FITC and p47phox-Alexa 546 conjugated were analysed by immunofluorescence. mRNA levels of p47phox (NADPH oxidase subunit) were assessed using Real Time qRT-PCR. The adherence and intracellular viability of S. agalactiae in HUVECs with or without pre-treatment of DPI, apocynin (NADPH oxidase inhibitors), and LY294002 (PI3K inhibitor) were evaluated by penicillin/gentamicin exclusion. Phosphorylation of p47phox and Akt activation by S. agalactiae were evaluated by immunoblotting analysis. FINDINGS Data showed increased ROS production 15 min after HUVEC infection. Real-Time qRT-PCR and western blotting performed in HUVEC infected with S. agalactiae detected alterations in mRNA levels and activation of p47phox. Pre-treatment of endothelial cells with NADPH oxidase (DPI and apocynin) and PI3K/Akt pathway (LY294002) inhibitors reduced ROS production, bacterial intracellular viability, and generation of actin stress fibres in HUVECs infected with S. agalactiae. CONCLUSIONS ROS generation via the NADPH oxidase pathway contributes to invasion of S. agalactiae in human endothelial cells accompanied by cytoskeletal reorganisation through the PI3K/Akt pathway, which provides novel evidence for the involvement of oxidative stress in S. agalactiae pathogenesis.
MeSH term(s)	Analysis of Variance ; Bacterial Adhesion ; Blotting, Western ; Human Umbilical Vein Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells/microbiology ; Humans ; NADPH Oxidases/analysis ; NADPH Oxidases/metabolism ; Oxidative Stress/physiology ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Reactive Oxygen Species/analysis ; Reactive Oxygen Species/metabolism ; Real-Time Polymerase Chain Reaction ; Reference Values ; Signal Transduction/physiology ; Streptococcal Infections/metabolism ; Streptococcus agalactiae/metabolism ; Time Factors
Chemical Substances	Reactive Oxygen Species ; NADPH Oxidases (EC 1.6.3.-) ; neutrophil cytosolic factor 1 (EC 1.6.3.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2018
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	953293-6
ISSN	1678-8060 ; 0074-0276
ISSN (online)	1678-8060
ISSN	0074-0276
DOI	10.1590/0074-02760170421
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