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  1. Article ; Online: Resveratrol as a Promising Nutraceutical: Implications in Gut Microbiota Modulation, Inflammatory Disorders, and Colorectal Cancer.

    Prakash, Vidhya / Bose, Chinchu / Sunilkumar, Damu / Cherian, Robin Mathew / Thomas, Shwetha Susan / Nair, Bipin G

    International journal of molecular sciences

    2024  Volume 25, Issue 6

    Abstract: Natural products have been a long-standing source for exploring health-beneficial components from time immemorial. Modern science has had a renewed interest in natural-products-based drug discovery. The quest for new potential secondary metabolites or ... ...

    Abstract Natural products have been a long-standing source for exploring health-beneficial components from time immemorial. Modern science has had a renewed interest in natural-products-based drug discovery. The quest for new potential secondary metabolites or exploring enhanced activities for existing molecules remains a pertinent topic for research. Resveratrol belongs to the stilbenoid polyphenols group that encompasses two phenol rings linked by ethylene bonds. Several plant species and foods, including grape skin and seeds, are the primary source of this compound. Resveratrol is known to possess potent anti-inflammatory, antiproliferative, and immunoregulatory properties. Among the notable bioactivities associated with resveratrol, its pivotal role in safeguarding the intestinal barrier is highlighted for its capacity to prevent intestinal inflammation and regulate the gut microbiome. A better understanding of how oxidative stress can be controlled using resveratrol and its capability to protect the intestinal barrier from a gut microbiome perspective can shed more light on associated physiological conditions. Additionally, resveratrol exhibits antitumor activity, proving its potential for cancer treatment and prevention. Moreover, cardioprotective, vasorelaxant, phytoestrogenic, and neuroprotective benefits have also been reported. The pharmaceutical industry continues to encounter difficulties administering resveratrol owing to its inadequate bioavailability and poor solubility, which must be addressed simultaneously. This report summarizes the currently available literature unveiling the pharmacological effects of resveratrol.
    MeSH term(s) Humans ; Resveratrol/pharmacology ; Resveratrol/therapeutic use ; Gastrointestinal Microbiome ; Polyphenols/pharmacology ; Dietary Supplements ; Colorectal Neoplasms/drug therapy
    Chemical Substances Resveratrol (Q369O8926L) ; Polyphenols
    Language English
    Publishing date 2024-03-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25063370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A novel and improved selective media for the isolation and enumeration of Klebsiella species

    Prasad, Megha / Shetty, Sindhu K. / Nair, Bipin G. / Pal, Sanjay / Madhavan, Ajith

    Appl Microbiol Biotechnol. 2022 Dec., v. 106, no. 24 p.8273-8284

    2022  

    Abstract: Bacterial pathogens are fostered in and transmitted through wastewater. Hence, monitoring their impact on sanitation and hygiene is imperative. As part of the monitoring process, culture-based methodologies are primarily used, which centre on the use of ... ...

    Abstract Bacterial pathogens are fostered in and transmitted through wastewater. Hence, monitoring their impact on sanitation and hygiene is imperative. As part of the monitoring process, culture-based methodologies are primarily used, which centre on the use of selective and differential media. Media available today are, at best, difficult to formulate and, at worst, prohibitively expensive. To address this lacuna, the study proposes a selective and differential medium for Klebsiella spp. Klebsiella blue agar (KBA) is completely selective against selected gram-positive bacteria (Bacillus spp., Staphylococcus aureus) and a few gram-negative bacteria (Acinetobacter baumanii, Serratia marcescens). On the other hand, it supports the growth of the chosen members of the Klebsiella pneumoniae species-complex with a characteristic green colouration. Methylene blue, tryptophan, and bile salt make up the selective components of KBA. Moreover, methylene blue, 0.6% NaCl, and glycerol render it differential. KBA was more selective than HiCrome™ Klebsiella Selective Agar Base (KSA) in replica plating experiments. KBA promoted only 157 CFUs against 209 CFUs in KSA when stamped with 253 CFUs grown on LB. The colonies so isolated were predominantly Klebsiella spp., on identification through colony polymerase chain reaction. Moreover, the differential nature of KBA distinguished Klebsiella aerogenes from other species. On the contrary, KSA lodged colonies indistinguishable from each other and Klebsiella spp. Due to its ease of formulation, high selectivity, differential nature, and cost-effective composition, KBA is a viable option for the routine culture of Klebsiella spp. in environmental and clinical settings. KEY POINTS: • Formulated a novel selective and differential media for Klebsiella spp., named Klebsiella Blue agar • Facile formulation methodology • Can be employed to isolate Klebsiella spp. from complex sources such as wastewater
    Keywords Acinetobacter ; Bacillus (bacteria) ; Klebsiella aerogenes ; Klebsiella pneumoniae ; Serratia marcescens ; Staphylococcus aureus ; agar ; bile salts ; color ; cost effectiveness ; glycerol ; hygiene ; methylene blue ; polymerase chain reaction ; sanitation ; tryptophan ; wastewater
    Language English
    Dates of publication 2022-12
    Size p. 8273-8284.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-022-12270-w
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Anacardic acid-mediated regulation of osteoblast differentiation involves mitigation of inflammasome activation pathways.

    Venugopal, Meera / Nambiar, Jyotsna / Nair, Bipin G

    Molecular and cellular biochemistry

    2020  Volume 476, Issue 2, Page(s) 819–829

    Abstract: Disruption of the finely tuned osteoblast-osteoclast balance is the underlying basis of several inflammatory bone diseases, such as osteomyelitis, osteoporosis, and septic arthritis. Prolonged and unrestrained exposure to inflammatory environment results ...

    Abstract Disruption of the finely tuned osteoblast-osteoclast balance is the underlying basis of several inflammatory bone diseases, such as osteomyelitis, osteoporosis, and septic arthritis. Prolonged and unrestrained exposure to inflammatory environment results in reduction of bone mineral density by downregulating osteoblast differentiation. Earlier studies from our laboratory have identified that Anacardic acid (AA), a constituent of Cashew nut shell liquid that is used widely in traditional medicine, has potential inhibitory effect on gelatinases (MMP2 and MMP9) which are over-expressed in numerous inflammatory conditions (Omanakuttan et al. in Mol Pharmacol, 2012 and Nambiar et al. in Exp Cell Res, 2016). The study demonstrated for the first time that AA promotes osteoblast differentiation in lipopolysaccharide-treated osteosarcoma cells (MG63) by upregulating specific markers, like osteocalcin, receptor activator of NF-κB ligand, and alkaline phosphatase. Furthermore, expression of the negative regulators, such as nuclear factor-κB, matrix metalloproteinases (MMPs), namely MMP13, and MMP1, along with several inflammatory markers, such as Interleukin-1β and Nod-like receptor protein 3 were downregulated by AA. Taken together, AA expounds as a novel template for development of potential pharmacological therapeutics for inflammatory bone diseases.
    MeSH term(s) Anacardic Acids/pharmacology ; Bone Diseases/drug therapy ; Bone Diseases/metabolism ; Bone Diseases/pathology ; Cell Differentiation/drug effects ; Cell Line, Tumor ; Humans ; Inflammasomes/antagonists & inhibitors ; Inflammasomes/metabolism ; NF-kappa B/antagonists & inhibitors ; Osteoblasts/drug effects ; Osteoblasts/metabolism ; Osteocalcin/agonists ; Osteocalcin/metabolism ; RANK Ligand/agonists ; RANK Ligand/metabolism
    Chemical Substances Anacardic Acids ; BGLAP protein, human ; Inflammasomes ; NF-kappa B ; RANK Ligand ; TNFSF11 protein, human ; Osteocalcin (104982-03-8) ; anacardic acid (18654-18-7)
    Language English
    Publishing date 2020-10-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 184833-1
    ISSN 1573-4919 ; 0300-8177
    ISSN (online) 1573-4919
    ISSN 0300-8177
    DOI 10.1007/s11010-020-03947-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Snake antivenom: Challenges and alternate approaches.

    Alangode, Aswathy / Rajan, Karthika / Nair, Bipin G

    Biochemical pharmacology

    2020  Volume 181, Page(s) 114135

    Abstract: Snake envenomation is still a serious threat to many countries in the world. The only mainstay treatment depends on the administration of animal derived immunoglobulin based antivenom. Significant limitations to these antivenoms are a challenge in the ... ...

    Abstract Snake envenomation is still a serious threat to many countries in the world. The only mainstay treatment depends on the administration of animal derived immunoglobulin based antivenom. Significant limitations to these antivenoms are a challenge in the treatment of snake envenomation. Many alternate approaches have been explored to overcome the limitations of antivenom. Exploring alternate approaches like use of bioactive components from plant sources, use of peptide and small molecule inhibitors are some aspects taken towards improving the current limitations of antivenom therapy. However, all these alternate approaches also have many drawbacks which should be improved by more in vitro and in vivo experiments. Here, we review some of the limitations of current antivenom therapy and developments as well as drawbacks of these alternate treatment strategies.
    MeSH term(s) Animals ; Antibodies, Neutralizing/immunology ; Antivenins/immunology ; Antivenins/therapeutic use ; Humans ; Peptides/immunology ; Peptides/therapeutic use ; Snake Bites/drug therapy ; Snake Bites/immunology ; Snake Venoms/antagonists & inhibitors ; Snake Venoms/immunology ; Snakes/immunology ; Snakes/metabolism ; Toxins, Biological/antagonists & inhibitors ; Toxins, Biological/immunology
    Chemical Substances Antibodies, Neutralizing ; Antivenins ; Peptides ; Snake Venoms ; Toxins, Biological
    Language English
    Publishing date 2020-07-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 208787-x
    ISSN 1873-2968 ; 0006-2952
    ISSN (online) 1873-2968
    ISSN 0006-2952
    DOI 10.1016/j.bcp.2020.114135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Systematic understanding of anti-tumor mechanisms of Tamarixetin through network and experimental analyses.

    Shaji, Sanu K / Drishya, G / Sunilkumar, Damu / Suravajhala, Prashanth / Kumar, Geetha B / Nair, Bipin G

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 3966

    Abstract: Tamarixetin, a flavonoid derived from Quercetin, was shown to possess anti-cancer properties in various types of cancer. However, the mechanism of action of this compound is not well understood. Observations from reverse docking and network pharmacology ... ...

    Abstract Tamarixetin, a flavonoid derived from Quercetin, was shown to possess anti-cancer properties in various types of cancer. However, the mechanism of action of this compound is not well understood. Observations from reverse docking and network pharmacology analysis, were validated by cell based studies to analyse the chemotherapeutic potential and elucidate the molecular mechanism of action of Tamarixetin in breast cancer. In silico analysis using reverse docking and PPI analysis clearly indicated that out of 35 proteins targeted by Tamarixetin, the top 3 hub genes, namely, AKT1, ESR1 and HSP90AA1, were upregulated in breast tumor tissues and more importantly showed strong negative correlation to breast cancer patient survival. Furthermore, the KEGG pathway analysis showed enrichment of target proteins of Tamarixetin in 33 pathways which are mainly involved in neoplastic signalling. In vitro cell-based studies demonstrated that Tamarixetin could inhibit cell proliferation, induce ROS and reduce mitochondrial membrane potential, leading to cell death. Tamarixetin induced cell cycle arrest at G2/M phase and inhibited the migration as well as the invasion of breast cancer cells. Taken together, the combination of in silico and in vitro approaches used in the present study clearly provides evidence for the chemotherapeutic potential of Tamarixetin in breast cancer.
    MeSH term(s) Breast Neoplasms/drug therapy ; Disaccharides/pharmacology ; Drugs, Chinese Herbal/pharmacology ; Female ; Humans ; Molecular Docking Simulation ; Quercetin/analogs & derivatives ; Quercetin/pharmacology ; Quercetin/therapeutic use
    Chemical Substances Disaccharides ; Drugs, Chinese Herbal ; tamarixetin (73WRA8Z8M8) ; Quercetin (9IKM0I5T1E)
    Language English
    Publishing date 2022-03-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-07087-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A novel and improved selective media for the isolation and enumeration of Klebsiella species.

    Prasad, Megha / Shetty, Sindhu K / Nair, Bipin G / Pal, Sanjay / Madhavan, Ajith

    Applied microbiology and biotechnology

    2022  Volume 106, Issue 24, Page(s) 8273–8284

    Abstract: Bacterial pathogens are fostered in and transmitted through wastewater. Hence, monitoring their impact on sanitation and hygiene is imperative. As part of the monitoring process, culture-based methodologies are primarily used, which centre on the use of ... ...

    Abstract Bacterial pathogens are fostered in and transmitted through wastewater. Hence, monitoring their impact on sanitation and hygiene is imperative. As part of the monitoring process, culture-based methodologies are primarily used, which centre on the use of selective and differential media. Media available today are, at best, difficult to formulate and, at worst, prohibitively expensive. To address this lacuna, the study proposes a selective and differential medium for Klebsiella spp. Klebsiella blue agar (KBA) is completely selective against selected gram-positive bacteria (Bacillus spp., Staphylococcus aureus) and a few gram-negative bacteria (Acinetobacter baumanii, Serratia marcescens). On the other hand, it supports the growth of the chosen members of the Klebsiella pneumoniae species-complex with a characteristic green colouration. Methylene blue, tryptophan, and bile salt make up the selective components of KBA. Moreover, methylene blue, 0.6% NaCl, and glycerol render it differential. KBA was more selective than HiCrome™ Klebsiella Selective Agar Base (KSA) in replica plating experiments. KBA promoted only 157 CFUs against 209 CFUs in KSA when stamped with 253 CFUs grown on LB. The colonies so isolated were predominantly Klebsiella spp., on identification through colony polymerase chain reaction. Moreover, the differential nature of KBA distinguished Klebsiella aerogenes from other species. On the contrary, KSA lodged colonies indistinguishable from each other and Klebsiella spp. Due to its ease of formulation, high selectivity, differential nature, and cost-effective composition, KBA is a viable option for the routine culture of Klebsiella spp. in environmental and clinical settings. KEY POINTS: • Formulated a novel selective and differential media for Klebsiella spp., named Klebsiella Blue agar • Facile formulation methodology • Can be employed to isolate Klebsiella spp. from complex sources such as wastewater.
    Language English
    Publishing date 2022-11-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-022-12270-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Escherichia coli ST155 as a production-host of three different polyvalent phages and their characterisation with a prospect for wastewater disinfection.

    Salim, Amrita / Madhavan, Ajith / Subhash, Suja / Prasad, Megha / Nair, Bipin G / Pal, Sanjay

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 19406

    Abstract: Bacteriophages are generally specific, and a cocktail of phages is needed to combat different bacterial targets. Their production usually requires pathogenic isolation hosts. We identified a novel strain, Escherichia coli ST155, that could serve as a ... ...

    Abstract Bacteriophages are generally specific, and a cocktail of phages is needed to combat different bacterial targets. Their production usually requires pathogenic isolation hosts. We identified a novel strain, Escherichia coli ST155, that could serve as a production host for three different polyvalent phages (ϕPh_SE03, ϕPh_SD01, and ϕPh_EC01), thus superseding the use of individual isolation hosts. Upon propagation in E. coli ST155, the phages demonstrated differential intergeneric infectivity against Salmonella enterica, E. coli OP50, Shigella dysenteriae, E. coli MDR, and Acinetobacter baumannii. Phages were characterised based on morphology, latent period, burst size, the efficiency of plating, and restriction enzyme profile. Survival assay on Caenorhabditis elegans, the absence of Shiga toxin, and enterotoxigenic E. coli virulence genes indicated that E. coli ST155 could be non-pathogenic. Lack of antibiotic resistance and absence of functional prophages rendered the host suitable for environmental applications. As a proof-of-concept, phage ϕPh_SE03 was produced in ST155 by employing a unique Bacteriophage Amplification Reactor-Lytics Broadcasting System and was simultaneously disseminated into S. enterica augmented wastewater, which resulted in a 3-log reduction in 24 h. The study establishes the potential of E. coli ST155 as a phage production host thereby minimising the possibility of accidental release of pathogenic hosts into wastewater.
    MeSH term(s) Humans ; Bacteriophages/genetics ; Escherichia coli ; Waste Water ; Clonidine ; Disinfection ; Escherichia coli Infections
    Chemical Substances Waste Water ; Clonidine (MN3L5RMN02)
    Language English
    Publishing date 2022-11-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-24134-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Zirconium copper oxide microflowers based non-enzymatic screen-printed electrochemical sensor for the detection of glucose in saliva, urine, and blood serum.

    J P, Chandhana / Punnakkal, Navaneeth / Vasu, Suneesh Punathil / Pradeep, Aarathi / Nair, Bipin G / Babu, T G Satheesh

    Mikrochimica acta

    2023  Volume 190, Issue 10, Page(s) 390

    Abstract: Zirconium copper oxide microflowers (Zr/CuO MF) based non-enzymatic sensor was developed for glucose detection in saliva, urine, and blood. An easy urea hydrolysis method was employed for the synthesis of the metal oxide and further calcined to improve ... ...

    Abstract Zirconium copper oxide microflowers (Zr/CuO MF) based non-enzymatic sensor was developed for glucose detection in saliva, urine, and blood. An easy urea hydrolysis method was employed for the synthesis of the metal oxide and further calcined to improve the catalytic property. The flower-like morphology of the Zr/CuO was confirmed by SEM analysis and the presence of copper and zirconium was examined using energy dispersive X-ray analysis (EDAX). The Zr/CuO MF modified screen-printed electrodes exhibited excellent glucose sensing performance in 0.15 M NaOH medium and could quantify glucose in the range from 10 µM to 27 mM. A high sensitivity of 1.815 ± 0.003 mA mM
    MeSH term(s) Saliva ; Serum ; Copper ; Glucose ; Zirconium ; Reproducibility of Results ; Body Fluids ; Oxides
    Chemical Substances cupric oxide (V1XJQ704R4) ; cuprous oxide (T8BEA5064F) ; Copper (789U1901C5) ; Glucose (IY9XDZ35W2) ; zirconium oxide (S38N85C5G0) ; Zirconium (C6V6S92N3C) ; Oxides
    Language English
    Publishing date 2023-09-12
    Publishing country Austria
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 89-9
    ISSN 1436-5073 ; 0026-3672
    ISSN (online) 1436-5073
    ISSN 0026-3672
    DOI 10.1007/s00604-023-05965-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Corrigendum: Paraptosis: a unique cell death mode for targeting cancer.

    Hanson, Sweata / Dharan, Aiswarya / V, Jinsha P / Pal, Sanjay / Nair, Bipin G / Kar, Rekha / Mishra, Nandita

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1274076

    Abstract: This corrects the article DOI: 10.3389/fphar.2023.1159409.]. ...

    Abstract [This corrects the article DOI: 10.3389/fphar.2023.1159409.].
    Language English
    Publishing date 2023-09-08
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1274076
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Paraptosis: a unique cell death mode for targeting cancer.

    Hanson, Sweata / Dharan, Aiswarya / P V, Jinsha / Pal, Sanjay / Nair, Bipin G / Kar, Rekha / Mishra, Nandita

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1159409

    Abstract: Programmed cell death (PCD) is the universal process that maintains cellular homeostasis and regulates all living systems' development, health and disease. Out of all, apoptosis is one of the major PCDs that was found to play a crucial role in many ... ...

    Abstract Programmed cell death (PCD) is the universal process that maintains cellular homeostasis and regulates all living systems' development, health and disease. Out of all, apoptosis is one of the major PCDs that was found to play a crucial role in many disease conditions, including cancer. The cancer cells acquire the ability to escape apoptotic cell death, thereby increasing their resistance towards current therapies. This issue has led to the need to search for alternate forms of programmed cell death mechanisms. Paraptosis is an alternative cell death pathway characterized by vacuolation and damage to the endoplasmic reticulum and mitochondria. Many natural compounds and metallic complexes have been reported to induce paraptosis in cancer cell lines. Since the morphological and biochemical features of paraptosis are much different from apoptosis and other alternate PCDs, it is crucial to understand the different modulators governing it. In this review, we have highlighted the factors that trigger paraptosis and the role of specific modulators in mediating this alternative cell death pathway. Recent findings include the role of paraptosis in inducing anti-tumour T-cell immunity and other immunogenic responses against cancer. A significant role played by paraptosis in cancer has also scaled its importance in knowing its mechanism. The study of paraptosis in xenograft mice, zebrafish model, 3D cultures, and novel paraptosis-based prognostic model for low-grade glioma patients have led to the broad aspect and its potential involvement in the field of cancer therapy. The co-occurrence of different modes of cell death with photodynamic therapy and other combinatorial treatments in the tumour microenvironment are also summarized here. Finally, the growth, challenges, and future perspectives of paraptosis research in cancer are discussed in this review. Understanding this unique PCD pathway would help to develop potential therapy and combat chemo-resistance in various cancer.
    Language English
    Publishing date 2023-06-15
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1159409
    Database MEDical Literature Analysis and Retrieval System OnLINE

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