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  1. Article: Highly Efficient Synthesis of Rare Sugars from Glycerol in Endotoxin-Free

    Gao, Yahui / Chen, Zhou / Nakanishi, Hideki / Li, Zijie

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 16

    Abstract: Rare sugars possess potential applications as low-calorie sweeteners, especially for anti-obesity and anti-diabetes. In this study, a fermentation biosystem based on the "DHAP-dependent aldolases strategy" was established for D-allulose and D-sorbose ... ...

    Abstract Rare sugars possess potential applications as low-calorie sweeteners, especially for anti-obesity and anti-diabetes. In this study, a fermentation biosystem based on the "DHAP-dependent aldolases strategy" was established for D-allulose and D-sorbose production from glycerol in endotoxin-free
    Language English
    Publishing date 2023-08-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12163078
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Highly Efficient Synthesis of Rare Sugars from Glycerol in Endotoxin-Free ClearColi by Fermentation

    Gao, Yahui / Chen, Zhou / Nakanishi, Hideki / Li, Zijie

    Foods. 2023 Aug. 16, v. 12, no. 16

    2023  

    Abstract: Rare sugars possess potential applications as low-calorie sweeteners, especially for anti-obesity and anti-diabetes. In this study, a fermentation biosystem based on the “DHAP-dependent aldolases strategy” was established for D-allulose and D-sorbose ... ...

    Abstract Rare sugars possess potential applications as low-calorie sweeteners, especially for anti-obesity and anti-diabetes. In this study, a fermentation biosystem based on the “DHAP-dependent aldolases strategy” was established for D-allulose and D-sorbose production from glycerol in endotoxin-free ClearColi BL21 (DE3). Several engineering strategies were adopted to enhance rare sugar production. Firstly, the combination of different plasmids for aldO, rhaD, and yqaB expression was optimized. Then, the artificially constructed ribosomal binding site (RBS) libraries of aldO, rhaD, and yqaB genes were assembled individually and combinatorially. In addition, a peroxidase was overexpressed to eliminate the damage or toxicity from hydrogen peroxide generated by alditol oxidase (AldO). Finally, stepwise improvements in rare sugar synthesis were elevated to 15.01 g/L with a high yield of 0.75 g/g glycerol in a 3 L fermenter. This research enables the effective production of rare sugars from raw glycerol in high yields.
    Keywords fermentation ; fermenters ; glycerol ; hydrogen peroxide ; peroxidase ; plasmids ; psicose ; sorbose ; toxicity
    Language English
    Dates of publication 2023-0816
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12163078
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Construction of a Yeast Cell-Based Assay System to Analyze SNAP25-Targeting Botulinum Neurotoxins.

    Chen, Shilin / Li, Feng / Liu, Guoyu / Li, Yuqing / Li, Zijie / Liu, Yishi / Nakanishi, Hideki

    Microorganisms

    2023  Volume 11, Issue 5

    Abstract: Herein, we describe a yeast cell-based assay system to analyze SNAP25-targeting botulinum neurotoxins (BoNTs). BoNTs are protein toxins, and, upon incorporation into neuronal cells, their light chains (BoNT-LCs) target specific synaptosomal N- ... ...

    Abstract Herein, we describe a yeast cell-based assay system to analyze SNAP25-targeting botulinum neurotoxins (BoNTs). BoNTs are protein toxins, and, upon incorporation into neuronal cells, their light chains (BoNT-LCs) target specific synaptosomal N-ethylmaleimide-sensitive attachment protein receptor (SNARE) proteins, including synaptosomal-associated protein 25 (SNAP25). BoNT-LCs are metalloproteases, and each BoNT-LC recognizes and cleaves conserved domains in SNAREs termed the SNARE domain. In the budding yeast
    Language English
    Publishing date 2023-04-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11051125
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  4. Article ; Online: Studies on the Proteinaceous Structure Present on the Surface of the

    Yang, Yan / Yang, Ganglong / Li, Zi-Jie / Liu, Yi-Shi / Gao, Xiao-Dong / Nakanishi, Hideki

    Journal of fungi (Basel, Switzerland)

    2023  Volume 9, Issue 4

    Abstract: The surface of ... ...

    Abstract The surface of the
    Language English
    Publishing date 2023-03-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof9040392
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  5. Article ; Online: Highly efficient expression and secretion of human lysozyme using multiple strategies in Pichia pastoris.

    Wang, Yasen / Wang, Buqing / Gao, Yahui / Nakanishi, Hideki / Gao, Xiao-Dong / Li, Zijie

    Biotechnology journal

    2023  Volume 18, Issue 11, Page(s) e2300259

    Abstract: Background: Human lysozyme (hLYZ), an emerging antibacterial agent, has extensive application in the food and pharmaceutical industries. However, the source of hLYZ is particularly limited.: Results: To achieve highly efficient expression and ... ...

    Abstract Background: Human lysozyme (hLYZ), an emerging antibacterial agent, has extensive application in the food and pharmaceutical industries. However, the source of hLYZ is particularly limited.
    Results: To achieve highly efficient expression and secretion of hLYZ in Pichia pastoris, multiple strategies including G418 sulfate screening, signal sequence optimization, vacuolar sorting receptor VPS10 disruption, and chaperones/transcription factors co-expression were applied. The maximal enzyme activity of extracellular hLYZ in a shaking flask was 81,600 ± 5230 U mL
    Conclusion: This research provides a very useful and cost-effective approach for the hLYZ production in P. pastoris and can also be applied to the production of other recombinant proteins.
    MeSH term(s) Humans ; Muramidase/genetics ; Pichia ; Recombinant Proteins/metabolism ; Saccharomycetales/metabolism
    Chemical Substances Muramidase (EC 3.2.1.17) ; Recombinant Proteins
    Language English
    Publishing date 2023-07-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.202300259
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6349: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Rare sugar L-sorbose exerts antitumor activity by impairing glucose metabolism.

    Xu, Hui-Lin / Zhou, Xiaoman / Chen, Shuai / Xu, Si / Li, Zijie / Nakanishi, Hideki / Gao, Xiao-Dong

    Communications biology

    2023  Volume 6, Issue 1, Page(s) 259

    Abstract: Rare sugars are monosaccharides with low natural abundance. They are structural isomers of dietary sugars, but hardly be metabolized. Here, we report that rare sugar L-sorbose induces apoptosis in various cancer cells. As a C-3 epimer of D-fructose, L- ... ...

    Abstract Rare sugars are monosaccharides with low natural abundance. They are structural isomers of dietary sugars, but hardly be metabolized. Here, we report that rare sugar L-sorbose induces apoptosis in various cancer cells. As a C-3 epimer of D-fructose, L-sorbose is internalized via the transporter GLUT5 and phosphorylated by ketohexokinase (KHK) to produce L-sorbose-1-phosphate (S-1-P). Cellular S-1-P inactivates the glycolytic enzyme hexokinase resulting in attenuated glycolysis. Consequently, mitochondrial function is impaired and reactive oxygen species are produced. Moreover, L-sorbose downregulates the transcription of KHK-A, a splicing variant of KHK. Since KHK-A is a positive inducer of antioxidation genes, the antioxidant defense mechanism in cancer cells can be attenuated by L-sorbose-treatment. Thus, L-sorbose performs multiple anticancer activities to induce cell apoptosis. In mouse xenograft models, L-sorbose enhances the effect of tumor chemotherapy in combination with other anticancer drugs. These results demonstrate L-sorbose as an attractive therapeutic reagent for cancer treatment.
    MeSH term(s) Humans ; Mice ; Animals ; Sorbose/metabolism ; Sorbose/pharmacology ; Sugars ; Fructose/metabolism ; Glycolysis ; Glucose
    Chemical Substances Sorbose (NV2001607Y) ; Sugars ; Fructose (30237-26-4) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2023-03-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-023-04638-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Establishment of a Novel Cell Surface Display Platform Based on Natural “Chitosan Beads” of Yeast Spores

    Li, Zijie / Li, Wanjie / Wang, Yasen / Chen, Zhou / Nakanishi, Hideki / Xu, Xiangyang / Gao, Xiao-Dong

    Journal of agricultural and food chemistry. 2022 June 09, v. 70, no. 24

    2022  

    Abstract: Cell surface display technology, which expresses and anchors proteins on the surface of microbial cells, has broad application prospects in many fields, such as protein library screening, biocatalysis, and biosensor development. However, traditional cell ...

    Abstract Cell surface display technology, which expresses and anchors proteins on the surface of microbial cells, has broad application prospects in many fields, such as protein library screening, biocatalysis, and biosensor development. However, traditional cell surface display systems have disadvantages: the molecular weight of phage display proteins cannot be too large; bacterial display lacks the post-translational modification process for eukaryotic proteins; yeast display is prone to excessive protein glycosylation and misfolding of multisubunit proteins; and the compatibility of Bacillus subtilis spore display needs to be further improved. Therefore, it is extremely valuable to develop an efficient surface display platform with strong universality and stress resistance properties. Although yeast surface display systems have been extensively investigated, the establishment of a surface display platform using yeast spores has rarely been reported. In this study, a novel cell surface display platform based on natural “chitosan beads” of yeast spores was developed. The target protein in fusion with the chitosan affinity protein (CAP) exhibited strong binding capability with “chitosan beads” of yeast spores in vitro and in vivo. Moreover, this protein display system showed highly preferable enzymatic properties and stability. As an example, the displayed LXYL-P1-2-CAP demonstrated high thermostability and reusability (60% of the initial activity after seven cycles of reuse), high storage stability (75% of original activity after 8 weeks), and excellent tolerance to a concentration up to 75% (v/v) organic reagents. To prove the practicability of this surface display system, the semisynthesis of paclitaxel intermediate was demonstrated and its highest conversion rate was 92% using 0.25 mM substrate. This study provides a novel and useful platform for the surface display of proteins, especially for multimeric macromolecular proteins of eukaryotic origin.
    Keywords Bacillus subtilis ; bacteriophages ; biocatalysis ; biosensors ; chitosan ; food chemistry ; glycosylation ; molecular weight ; paclitaxel ; spores ; storage quality ; stress tolerance ; thermal stability ; yeasts
    Language English
    Dates of publication 2022-0609
    Size p. 7479-7489.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.2c01983
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    In stock of ZB MED Bonn / Germany

    Z 1185: Show issues

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  8. Article ; Online: Encapsulation of Mannose-6-phosphate Isomerase in Yeast Spores and Its Application in l-Ribose Production.

    Li, Zijie / Liu, Xiaoxiao / Nakanishi, Hideki / Gao, Xiao-Dong

    Journal of agricultural and food chemistry

    2020  Volume 68, Issue 25, Page(s) 6892–6899

    Abstract: A mannose-6-phosphate isomerase (MPI) ... ...

    Abstract A mannose-6-phosphate isomerase (MPI) from
    MeSH term(s) Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Gene Expression ; Geobacillus/enzymology ; Mannose-6-Phosphate Isomerase/chemistry ; Mannose-6-Phosphate Isomerase/genetics ; Mannose-6-Phosphate Isomerase/metabolism ; Ribose/biosynthesis ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Spores, Fungal/genetics ; Spores, Fungal/metabolism
    Chemical Substances Bacterial Proteins ; Ribose (681HV46001) ; Mannose-6-Phosphate Isomerase (EC 5.3.1.8)
    Language English
    Publishing date 2020-06-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.0c02399
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Establishment of a Novel Cell Surface Display Platform Based on Natural "Chitosan Beads" of Yeast Spores.

    Li, Zijie / Li, Wanjie / Wang, Yasen / Chen, Zhou / Nakanishi, Hideki / Xu, Xiangyang / Gao, Xiao-Dong

    Journal of agricultural and food chemistry

    2022  Volume 70, Issue 24, Page(s) 7479–7489

    Abstract: Cell surface display technology, which expresses and anchors proteins on the surface of microbial cells, has broad application prospects in many fields, such as protein library screening, biocatalysis, and biosensor development. However, traditional cell ...

    Abstract Cell surface display technology, which expresses and anchors proteins on the surface of microbial cells, has broad application prospects in many fields, such as protein library screening, biocatalysis, and biosensor development. However, traditional cell surface display systems have disadvantages: the molecular weight of phage display proteins cannot be too large; bacterial display lacks the post-translational modification process for eukaryotic proteins; yeast display is prone to excessive protein glycosylation and misfolding of multisubunit proteins; and the compatibility of
    MeSH term(s) Bacillus subtilis/genetics ; Bacillus subtilis/metabolism ; Bacterial Proteins/metabolism ; Chitosan/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Spores, Bacterial/genetics ; Spores, Bacterial/metabolism ; Spores, Fungal/metabolism
    Chemical Substances Bacterial Proteins ; Chitosan (9012-76-4)
    Language English
    Publishing date 2022-06-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.2c01983
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Triosephosphate Isomerase and Its Product Glyceraldehyde-3-Phosphate Are Involved in the Regulatory Mechanism That Suppresses Exit from the Quiescent State in Yeast Cells.

    Liu, Guoyu / Yang, Yan / Yang, Ganglong / Duan, Shenglin / Yuan, Peng / Zhang, Shuang / Li, Feng / Gao, Xiao-Dong / Nakanishi, Hideki

    Microbiology spectrum

    2022  Volume 10, Issue 4, Page(s) e0089722

    Abstract: Cells of the budding yeast Saccharomyces cerevisiae form spores or stationary cells upon nutrient starvation. These quiescent cells are known to resume mitotic growth in response to nutrient signals, but the mechanism remains elusive. Here, we report ... ...

    Abstract Cells of the budding yeast Saccharomyces cerevisiae form spores or stationary cells upon nutrient starvation. These quiescent cells are known to resume mitotic growth in response to nutrient signals, but the mechanism remains elusive. Here, we report that quiescent yeast cells are equipped with a negative regulatory mechanism which suppresses the commencement of mitotic growth. The regulatory process involves a glycolytic enzyme, triosephosphate isomerase (Tpi1), and its product, glyceraldehyde-3-phosphate (GAP). GAP serves as an inhibitory signaling molecule; indeed, the return to growth of spores or stationary cells is suppressed by the addition of GAP even in nutrient-rich growth media, though mitotic cells are not affected. Reciprocally, dormancy is abolished by heat treatment because of the heat sensitivity of Tpi1. For example, spores commence germination merely upon heat treatment, which indicates that the negative regulatory mechanism is actively required for spores to prevent premature germination. Stationary cells of Candida glabrata are also manipulated by heat and GAP, suggesting that the regulatory process is conserved in the pathogenic yeast.
    MeSH term(s) Glyceraldehyde ; Glyceraldehyde 3-Phosphate ; Phosphates ; Saccharomyces cerevisiae ; Triose-Phosphate Isomerase/metabolism
    Chemical Substances Phosphates ; Glyceraldehyde 3-Phosphate (142-10-9) ; Glyceraldehyde (367-47-5) ; Triose-Phosphate Isomerase (EC 5.3.1.1)
    Language English
    Publishing date 2022-08-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.00897-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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