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  1. Article ; Online: Theorising post-truth in the COVID era.

    Nally, David

    Journal of educational change

    2022  Volume 23, Issue 2, Page(s) 277–289

    Abstract: The focus of this article is on the impacts of COVID-19 related manifestations of post-truth in educational settings in Australia. Within this context, there has been a reorientation of how wellbeing and academic achievement within schools reflect on ... ...

    Abstract The focus of this article is on the impacts of COVID-19 related manifestations of post-truth in educational settings in Australia. Within this context, there has been a reorientation of how wellbeing and academic achievement within schools reflect on broader trends within the general public, at local, state and national scales. Individual and communal experiences of adversity have been significantly impacted by phenomena associated with post-truth, particularly misinformation, a climate of anti-intellectualism, as well as fragmented socio-cultural cohesion. In the first section I explore these trends by providing an overview of how post-truth has been construed in Australian contexts, before moving to consider how engagements with post-truth have been shaped by the pandemic. Second, I analyse the close link between educational concerns that emerged from the pandemic era, and the circumstances that have supported the emergence of post-truth. Particular attention will be paid to debates over 'learning loss' and the place of teachers within Australian communities as a fulcrum for generating cultural capital and social cohesion. In the final section I consider what lessons these experiences have for education, as a way of cultivating learning communities that are oriented towards generating critical and digital literacy skills.
    Language English
    Publishing date 2022-04-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2016873-1
    ISSN 1573-1812 ; 1389-2843
    ISSN (online) 1573-1812
    ISSN 1389-2843
    DOI 10.1007/s10833-022-09457-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Development and Optimization of a Hydrophobic Interaction Chromatography-Based Method of AAV Harvest, Capture, and Recovery.

    McNally, David J / Piras, Bryan A / Willis, Catherine M / Lockey, Timothy D / Meagher, Michael M

    Molecular therapy. Methods & clinical development

    2020  Volume 19, Page(s) 275–284

    Abstract: With many ongoing clinical trials utilizing adeno-associated virus (AAV) gene therapy, it is necessary to find scalable and serotype-independent primary capture and recovery methods to allow for efficient and robust manufacturing processes. Here, we ... ...

    Abstract With many ongoing clinical trials utilizing adeno-associated virus (AAV) gene therapy, it is necessary to find scalable and serotype-independent primary capture and recovery methods to allow for efficient and robust manufacturing processes. Here, we demonstrate the ability of a hydrophobic interaction chromatography membrane to capture and recover AAV1, AAV5, AAV8, and AAV "Mutant C" (a novel serotype incorporating elements of AAV3B and AAV8) particles from cell culture media and cell lysate with recoveries of 76%-100% of loaded material, depending on serotype. A simple, novel technique that integrates release and recovery of cell-associated AAV capsids is demonstrated. We show that by the addition of lyotropic salts to AAV-containing cell suspensions, AAV is released at an equivalent efficiency to mechanical lysis. The addition of the lyotropic salt also promotes a phase separation, which allows physical removal of large amounts of DNA and insoluble cellular debris from the AAV-containing aqueous fraction. The AAV is then captured and eluted from a hydrophobic interaction chromatography membrane. This integrated lysis and primary capture and recovery technique facilitates substantial removal of host-cell DNA and host-cell protein impurities.
    Language English
    Publishing date 2020-09-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2872938-9
    ISSN 2329-0501 ; 2329-0501
    ISSN (online) 2329-0501
    ISSN 2329-0501
    DOI 10.1016/j.omtm.2020.09.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing.

    Fu, Qiang / Lee, Yong Suk / Green, Erica A / Wang, Yongdan / Park, So Young / Polanco, Ashli / Lee, Kelvin H / Betenbaugh, Michael / McNally, David / Yoon, Seongkyu

    Biotechnology and bioengineering

    2023  Volume 120, Issue 11, Page(s) 3148–3162

    Abstract: Recombinant adeno-associated virus (rAAV) vectors are a promising platform for in vivo gene therapies. However, cost-effective, well-characterized processes necessary to manufacture rAAV therapeutics are challenging to develop without an understanding of ...

    Abstract Recombinant adeno-associated virus (rAAV) vectors are a promising platform for in vivo gene therapies. However, cost-effective, well-characterized processes necessary to manufacture rAAV therapeutics are challenging to develop without an understanding of how process parameters (PPs) affect rAAV product quality attributes (PQAs). In this work, a central composite orthogonal experimental design was employed to examine the influence of four PPs for transient transfection complex formation (polyethylenimine:DNA [PEI:DNA] ratio, total DNA/cell, cocktail volume, and incubation time) on three rAAV PQAs related to capsid content (vector genome titer, vector genome:capsid particle ratio, and two-dimensional vector genome titer ratio). A regression model was established for each PQA using partial least squares, and a design space (DS) was defined in which Monte Carlo simulations predicted < 1% probability of failure (POF) to meet predetermined PQA specifications. Of the three PQAs, viral genome titer was most strongly correlated with changes in complexation PPs. The DS and acceptable PP ranges were largest when incubation time and cocktail volume were kept at mid-high setpoints, and PEI:DNA ratio and total DNA/cell were at low-mid setpoints. Verification experiments confirmed model predictive capability, and this work establishes a framework for studying other rAAV PPs and their relationship to PQAs.
    Language English
    Publishing date 2023-07-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.28508
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book: Global slump

    McNally, David

    the economics and politics of crisis and resistance

    (Spectre)

    2011  

    Author's details David McNally
    Series title Spectre
    Keywords Global Financial Crisis, 2008-2009 ; Finanzkrise ; Kapitalismus ; Finanzmarktregulierung ; Internationaler Finanzmarkt ; USA
    Language English
    Size XII, 230 S., graph. Darst.
    Publisher PM Press
    Publishing place Oakland, Calif
    Document type Book
    Note Includes bibliographical references
    ISBN 9781552663967 ; 9781604863321 ; 1552663965 ; 1604863323
    Database ECONomics Information System

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  5. Book ; Online: Monsters of the market

    McNally, David

    zombies, vampires, and global capitalism

    (Historical materialism book series ; 30)

    2011  

    Abstract: Monsters of the Market investigates the rise of capitalism through the prism of the body-panics it arouses. Drawing on folklore, literature and popular culture, the book links tales of monstrosity from early-modern England, including Mary Shelleys ... ...

    Institution ebrary, Inc
    Author's details by David McNally
    Series title Historical materialism book series ; 30
    Abstract Monsters of the Market investigates the rise of capitalism through the prism of the body-panics it arouses. Drawing on folklore, literature and popular culture, the book links tales of monstrosity from early-modern England, including Mary Shelleys Frankenstein, to a spate of recent vampire- and zombie-fables from sub-Saharan Africa, and it connects these to Marxs persistent use of monster-metaphors in his descriptions of capitalism. Reading across these tales of the grotesque, Monsters of the Market offers a novel account of the cultural and corporeal economy of a global market-system. The boo
    Keywords Capitalism ; Free enterprise ; Globalization/Economic aspects
    Language English
    Size Online-Ressource (x, 296 p), ill
    Publisher Brill
    Publishing place Leiden ;Boston
    Document type Book ; Online
    Note Includes bibliographical references and index
    ISBN 9789004201576 ; 9004201572
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  6. Book: Monsters of the market

    McNally, David

    zombies, vampires, and global capitalism

    (Historical materialism book series ; 30)

    2011  

    Author's details by David McNally
    Series title Historical materialism book series ; 30
    Keywords Capitalism/History ; Free enterprise ; Globalization/Economic aspects ; Kapitalismus ; Weltwirtschaftsordnung ; Kritik ; Marxismus ; Wirtschaftsgeschichte ; Großbritannien ; Afrika südlich der Sahara
    Language English
    Size X, 296 S.
    Publisher Haymarket Books ; Brill
    Publishing place Chicago, Ill ; Leiden u.a.
    Document type Book
    Note Includes bibliographical references and index
    ISBN 9781608462339 ; 9789004201576 ; 1608462331 ; 9004201572
    Database ECONomics Information System

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  7. Book: Monsters of the market

    McNally, David

    zombies, vampires, and global capitalism

    (Historical materialism book series ; 30)

    2011  

    Author's details by David McNally
    Series title Historical materialism book series ; 30
    Keywords Capitalism/History ; Free enterprise ; Globalization/Economic aspects ; Kapitalismus ; Weltwirtschaftsordnung ; Kritik ; Marxismus ; Wirtschaftsgeschichte ; Großbritannien ; Afrika südlich der Sahara
    Language English
    Size X, 296 S.
    Publisher Haymarket Books ; Brill
    Publishing place Chicago, Ill ; Leiden u.a.
    Document type Book
    Note Includes bibliographical references and index
    ISBN 9781608462339 ; 9789004201576 ; 1608462331 ; 9004201572
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  8. Article ; Online: Advancing a rapid, high throughput screening platform for optimization of lentivirus production.

    Gopal, Sneha / Osborne, Adam E / Hock, Lindsay / Zemianek, Jill / Fang, Kun / Gee, Gretchen / Ghosh, Ronit / McNally, David / Cramer, Steven M / Dordick, Jonathan S

    Biotechnology journal

    2021  Volume 16, Issue 10, Page(s) e2000621

    Abstract: Background: Lentiviral vectors (LVVs) hold great promise as delivery tools for gene therapy and chimeric antigen receptor T cell (CAR-T) therapy. Their ability to target difficult to transfect cells and deliver genetic payloads that integrate into the ... ...

    Abstract Background: Lentiviral vectors (LVVs) hold great promise as delivery tools for gene therapy and chimeric antigen receptor T cell (CAR-T) therapy. Their ability to target difficult to transfect cells and deliver genetic payloads that integrate into the host genome makes them ideal delivery candidates. However, several challenges remain to be addressed before LVVs are more widely used as therapeutics including low viral vector concentrations and the absence of suitable scale-up methods for large-scale production. To address these challenges, we have developed a high throughput microscale HEK293 suspension culture platform that enables rapid screening of conditions for improving LVV productivity.
    Key results: High density culture (40 million cells mL
    Conclusions: The ability to test a large number of conditions simultaneously with minimal reagent use allows for the rapid optimization of LVV production in HEK293 suspension cells. Therefore, such a system may serve as a valuable tool in early stage process development and can be used as a screening tool to improve LVV concentrations for both batch and perfusion based systems.
    MeSH term(s) Genetic Vectors/genetics ; HEK293 Cells ; High-Throughput Screening Assays ; Humans ; Lentivirus/genetics ; Transfection
    Language English
    Publishing date 2021-07-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.202000621
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Inequality, the profit system and global crisis

    McNally, David

    Bankruptcies and bailouts , p. 32-45

    2009  , Page(s) 32–45

    Author's details David McNally
    Language English
    Size graph. Darst.
    Publisher Fernwood Publ.
    Publishing place Halifax [u.a.]
    Document type Article
    ISBN 978-1-552-66313-4 ; 1-552-66313-2
    Database ECONomics Information System

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  10. Book ; Online: Be your own brand

    McNally, David / Speak, Karl D

    achieve more of what you want by being more of who you are

    (A BK life book)

    2011  

    Abstract: In this second edition of their classic book on personal brand David McNally and Karl Speak show that developing a personal brand is not about constructing a contrived image. Rather, it is a process of discovering who you really are and what you aspire ... ...

    Institution ebrary, Inc
    Author's details David McNally, Karl D. Speak
    Series title A BK life book
    Abstract In this second edition of their classic book on personal brand David McNally and Karl Speak show that developing a personal brand is not about constructing a contrived image. Rather, it is a process of discovering who you really are and what you aspire to be. The hallmark insight of this new edition is that the best way to establish a strong and memorable brand is to make a positive difference in the lives of others through making lasting impressions that build trusting relationships.McNally and Speak take you through the process of identifying the key components of your brand, conveying that
    Keywords Brand name products ; Identity (Psychology)
    Language English
    Size Online-Ressource (ix, 143 p), ports
    Edition 2nd ed., updated and expanded
    Publisher Berrett-Koehler Publishers
    Publishing place San Francisco, CA
    Document type Book ; Online
    Note Includes bibliographical references and index
    ISBN 9781605098104 ; 9781605098111 ; 1605098108 ; 1605098116
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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