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Article ; Online: Bone marrow adipocytes - Good, bad, or just different?

Nandy, Ananya / Rendina-Ruedy, Elizabeth

Best practice & research. Clinical endocrinology & metabolism

2021 Volume 35, Issue 4, Page(s) 101550

Abstract: Skeletal remodeling is essential for proper maintenance of adult bone mass, and due to its heavy energetic demands this process is closely tied to whole body metabolic. Thus, bone formation by the osteoblast, bone resorption by the osteoclast, and ... ...

Abstract	Skeletal remodeling is essential for proper maintenance of adult bone mass, and due to its heavy energetic demands this process is closely tied to whole body metabolic. Thus, bone formation by the osteoblast, bone resorption by the osteoclast, and mechano-sensing by the osteocyte, are highly coupled processes that are essential for bone turnover. When one experiences a disruption in these processes, over time increased skeletal fragility and fracture can result. In addition to these primary cells, secondary cells within the skeletal niche are suspected to directly coordinate bone health as well. The bone marrow compartment provides a unique microenvironment in which communication occurs between white blood cells, red blood cells, platelets, and immune cells, in addition to classic bone cells (osteoblasts, osteoclasts, and osteocytes) that can both directly and indirectly impact skeletal homeostasis. One such cell population that has attracted much attention and scientific inquiry in the past decade are bone marrow adipocytes (BMAdipo) which can be found interspersed throughout the marrow compartment, and collectively are often referred to as bone marrow adipose tissue (BMAT). Although our understanding of BMAT has advanced significantly in the past decade, many questions remain relative to their lineage and function. The current collection presents recent studies and the up-to-date understanding of bone marrow adipocytes, along with future clinical perspectives related to this unique depot.
MeSH term(s)	Adipocytes ; Adipose Tissue ; Bone Marrow ; Humans ; Osteoblasts ; Osteoclasts ; Osteocytes
Language	English
Publishing date	2021-05-29
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2052339-7
ISSN	1878-1594 ; 1532-1908 ; 1521-690X
ISSN (online)	1878-1594 ; 1532-1908
ISSN	1521-690X
DOI	10.1016/j.beem.2021.101550
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Article ; Online: Altered Osteoblast Metabolism with Aging Results in Lipid Accumulation and Oxidative Stress Mediated Bone Loss.

Nandy, Ananya / Richards, Alison / Thapa, Santosh / Akhmetshina, Alena / Narayani, Nikita / Rendina-Ruedy, Elizabeth

Aging and disease

2024 Volume 15, Issue 2, Page(s) 767–786

Abstract: Cellular aging is associated with dysfunction of numerous tissues affecting multiple organ systems. A striking example of this is related to age-related bone loss, or osteoporosis, increasing fracture incidence. Interestingly, the two compartments of ... ...

Abstract	Cellular aging is associated with dysfunction of numerous tissues affecting multiple organ systems. A striking example of this is related to age-related bone loss, or osteoporosis, increasing fracture incidence. Interestingly, the two compartments of bone, cortical and cancellous or trabecular, rely on different mechanisms for development and maintenance during 'normal' aging. At a cellular level, the aging process disturbs a multitude of intracellular pathways. In particular, alterations in cellular metabolic functions thereby impacting cellular bioenergetics have been implicated in multiple tissues. Therefore, this study aimed to characterize how metabolic processes were altered in bone forming osteoblasts in aged mice compared to young mice. Metabolic flux analyses demonstrated both stromal cells and mature, matrix secreting osteoblasts from aged mice exhibited mitochondrial dysfunction. This was also accompanied by a lack of adaptability or metabolic flexibility to utilize exogenous substrates compared to osteoblasts cultured from young mice. Additionally, lipid droplets accumulated in both early stromal cells and mature osteoblasts from aged mice, which was further depicted as increased lipid content within the bone cortex of aged mice. Global transcriptomic analysis of the bone further supported these metabolic data as enhanced oxidative stress genes were up-regulated in aged mice, while osteoblast-related genes were down-regulated when compared to the young mice. Collectively, these data suggest that aging results in altered osteoblast metabolic handling of both exogenous and endogenous substrates which could contribute to age-related osteoporosis.
MeSH term(s)	Mice ; Animals ; Osteoblasts/metabolism ; Bone and Bones/metabolism ; Osteoporosis/genetics ; Oxidative Stress ; Lipids
Chemical Substances	Lipids
Language	English
Publishing date	2024-04-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2625789-0
ISSN	2152-5250 ; 2152-5250
ISSN (online)	2152-5250
ISSN	2152-5250
DOI	10.14336/AD.2023.0510
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Article: Using real-time cell metabolic flux analyzer to monitor osteoblast bioenergetics

Jayapalan, Shobana / Nandy, Ananya / Rendina-Ruedy, Elizabeth

Journal of visualized experiments. 2022 Mar. 01, , no. 181

2022

Abstract: Bone formation by osteoblasts is an essential process for proper bone acquisition and bone turnover to maintain skeletal homeostasis, and ultimately, prevent fracture. In the interest to both optimize peak bone mass and combat various musculoskeletal ... ...

Abstract	Bone formation by osteoblasts is an essential process for proper bone acquisition and bone turnover to maintain skeletal homeostasis, and ultimately, prevent fracture. In the interest to both optimize peak bone mass and combat various musculoskeletal diseases (i.e., post-menopausal osteoporosis, anorexia nervosa, type 1 and 2 diabetes mellitus), incredible efforts have been made in the field of bone biology to fully characterize osteoblasts throughout their differentiation process. Given the primary role of mature osteoblasts to secrete matrix proteins and mineralization vesicles, it has been noted that these processes take an incredible amount of cellular energy, or adenosine triphosphate (ATP). The overall cellular energy status is often referred to as cellular bioenergetics, and it includes a series of metabolic reactions that sense substrate availability to derive ATP to meet cellular needs. Therefore, the current method details the process of isolating primary, murine bone marrow stromal cells (BMSCs) and monitoring their bioenergetic status using the Real-time cell metabolic flux analyzer at various stages in osteoblast differentiation. Importantly, these data have demonstrated that the metabolic profile changes dramatically throughout osteoblast differentiation. Thus, using this physiologically relevant cell type is required to fully appreciate how a cell's bioenergetic status can regulate the overall function.
Keywords	adenosine triphosphate ; anorexia nervosa ; bone density ; bone formation ; diabetes mellitus ; energy ; energy metabolism ; homeostasis ; mice ; mineralization ; osteoblasts ; osteoporosis ; postmenopause
Language	English
Dates of publication	2022-0301
Size	p. e63142.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/63142
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A more efficient valuation of beaches using tourists’ perspectives and Geographic Information System (GIS)

Bashar Raisa / Nandy Ananya

Environmental & Socio-economic Studies, Vol 7, Iss 3, Pp 54-

The case of Patenga of Chittagong, Bangladesh

2019 Volume 65

Abstract: The valuation of non–marketed goods and services of beaches in developing nations is rarely conducted; thus, lax management efforts at these resource–rich places often lead to degradation. In an attempt to resolve this issue the research used the travel ... ...

Abstract	The valuation of non–marketed goods and services of beaches in developing nations is rarely conducted; thus, lax management efforts at these resource–rich places often lead to degradation. In an attempt to resolve this issue the research used the travel cost valuation method and incorporated the use of geographic information system to estimate a never-before-done non-market valuation of the area visited by respondents at the beach of Patenga of Chittagong, Bangladesh. The uniqueness of the research stems from defining the area enjoyed by the survey respondents, via mapping, which helped assign the value generated to that particular part of Patenga and not the rest; this significantly decreased the underestimation problem of the travel cost methodology. The research used secondary sources and collected responses and geographical data from surveys conducted over a seven day period in March of 2018, from 277 respondents. A value of at least 14,331,044BDT/USD1.71million was estimated for 2018 for only 9% of Patenga. Furthermore, the Willingness to Pay approach was used to propose an entry fee of 15BDT/USD0.18 which would yield a revenue of more than 6,750,000BDT/USD79,882 around the year. The authors believe, these results will push the policy-makers and encourage stakeholder participation to better implement and monitor environmental management measures at Patenga.
Keywords	travel cost ; willingness to pay ; beach ; gis ; chittagong ; patenga ; Environmental sciences ; GE1-350
Subject code	333
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Endocrinal metabolic regulation on the skeletal system in post-menopausal women.

Thapa, Santosh / Nandy, Ananya / Rendina-Ruedy, Elizabeth

Frontiers in physiology

2022 Volume 13, Page(s) 1052429

Abstract: Osteoporosis is a common endocrinologic disorder characterized as a chronic bone loss condition. Sexual dimorphism is ubiquitous in the incidence of osteoporosis with post-menopausal women being acutely affected. Gonadal sex hormones including estrogen ... ...

Abstract	Osteoporosis is a common endocrinologic disorder characterized as a chronic bone loss condition. Sexual dimorphism is ubiquitous in the incidence of osteoporosis with post-menopausal women being acutely affected. Gonadal sex hormones including estrogen act as crucial regulators of bone mass; therefore, loss of such hormones leads to an imbalance in skeletal turnover leading to osteoporosis. Estrogen can influence both bone formation as well as resorption by reducing osteoblast activity and enhancing osteoclastogenesis. Additionally, estrogen is a potent regulator of systemic metabolism. Recent studies have provided clues that estrogenic effect on bone might also involve alterations in bone cell metabolism and bioenergetic potential. While direct effects of gonadal hormones ability to alter intracellular metabolism of bone cells has not been studied, there is precedence within the literature that this is occurring and contributing to post-menopausal bone loss. This review aims to serve as a perspective piece detailing the prospective role of gonadal hormones regulating bone cell metabolic potential.
Language	English
Publishing date	2022-11-11
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2022.1052429
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Article ; Online: Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics.

Jayapalan, Shobana / Nandy, Ananya / Rendina-Ruedy, Elizabeth

Journal of visualized experiments : JoVE

2022 , Issue 181

Abstract	Bone formation by osteoblasts is an essential process for proper bone acquisition and bone turnover to maintain skeletal homeostasis, and ultimately, prevent fracture. In the interest to both optimize peak bone mass and combat various musculoskeletal diseases (i.e., post-menopausal osteoporosis, anorexia nervosa, type 1 and 2 diabetes mellitus), incredible efforts have been made in the field of bone biology to fully characterize osteoblasts throughout their differentiation process. Given the primary role of mature osteoblasts to secrete matrix proteins and mineralization vesicles, it has been noted that these processes take an incredible amount of cellular energy, or adenosine triphosphate (ATP). The overall cellular energy status is often referred to as cellular bioenergetics, and it includes a series of metabolic reactions that sense substrate availability to derive ATP to meet cellular needs. Therefore, the current method details the process of isolating primary, murine bone marrow stromal cells (BMSCs) and monitoring their bioenergetic status using the Real-time cell metabolic flux analyzer at various stages in osteoblast differentiation. Importantly, these data have demonstrated that the metabolic profile changes dramatically throughout osteoblast differentiation. Thus, using this physiologically relevant cell type is required to fully appreciate how a cell's bioenergetic status can regulate the overall function.
MeSH term(s)	Animals ; Cell Differentiation ; Diabetes Mellitus, Type 1 ; Diabetes Mellitus, Type 2/metabolism ; Energy Metabolism/physiology ; Mice ; Osteoblasts ; Osteogenesis
Language	English
Publishing date	2022-03-01
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/63142
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Article ; Online: Environmental citizenship and solid waste management in Chattogram, Bangladesh

Biswas Sarmistha / Nandy Ananya / Islam Nafisa / Rafa Nazifa

Open Economics, Vol 3, Iss 1, Pp 135-

2020 Volume 150

Abstract: Chattogram city is grappling with problems caused by inadequate solid waste management (SWM). Because the successful policy intervention regarding proper SWM depends on the intrinsic motivation of the city dwellers along with government intervention, the ...

Abstract	Chattogram city is grappling with problems caused by inadequate solid waste management (SWM). Because the successful policy intervention regarding proper SWM depends on the intrinsic motivation of the city dwellers along with government intervention, the objective of this paper was to conduct an attitudinal study of the residents of Chattogram regarding their roles and responsibilities towards the SWM, and thus, the sustainable development of the city. It is a cross-sectional study based on a sample size of 150. Data was collected using a structured, pretested questionnaire. The result showed that the residents were unable to prioritize the city’s environmental problem over other problems due to the lack of knowledge and awareness about the environmental problems and its impacts on the Chattogram. However, in respect of SWM in the city, their eagerness for participation is appreciable, even though they are more dependent on government policy initiatives. An attitudinal gap was also observed here among the respondents pertaining to the vacuum of knowledge and awareness. Therefore, strong policy decisions and the awareness campaign can make people aware of their roles and responsibilities for a sustainable city and intrinsically motivate them to participate in SWM.
Keywords	behavior ; environmental citizenship ; solid waste management ; sustainable development ; sustainable city ; Economics as a science ; HB71-74
Subject code	710
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	De Gruyter
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Environmental citizenship and solid waste management in Chattogram, Bangladesh

Biswas, Sarmistha / Nandy, Ananya / Islam, Nafisa / Rafa, Nazifa

2020

Abstract	Chattogram city is grappling with problems caused by inadequate solid waste management (SWM). Because the successful policy intervention regarding proper SWM depends on the intrinsic motivation of the city dwellers along with government intervention, the objective of this paper was to conduct an attitudinal study of the residents of Chattogram regarding their roles and responsibilities towards the SWM, and thus, the sustainable development of the city. It is a cross-sectional study based on a sample size of 150. Data was collected using a structured, pretested questionnaire. The result showed that the residents were unable to prioritize the city's environmental problem over other problems due to the lack of knowledge and awareness about the environmental problems and its impacts on the Chattogram. However, in respect of SWM in the city, their eagerness for participation is appreciable, even though they are more dependent on government policy initiatives. An attitudinal gap was also observed here among the respondents pertaining to the vacuum of knowledge and awareness. Therefore, strong policy decisions and the awareness campaign can make people aware of their roles and responsibilities for a sustainable city and intrinsically motivate them to participate in SWM.
Keywords	ddc:330 ; behavior ; environmental citizenship ; solid waste management ; sustainable development ; sustainable city
Subject code	710
Language	English
Publisher	Warsaw: De Gruyter
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Lipolysis supports bone formation by providing osteoblasts with endogenous fatty acid substrates to maintain bioenergetic status.

Nandy, Ananya / Helderman, Ron C M / Thapa, Santosh / Jayapalan, Shobana / Richards, Alison / Narayani, Nikita / Czech, Michael P / Rosen, Clifford J / Rendina-Ruedy, Elizabeth

Bone research

2023 Volume 11, Issue 1, Page(s) 62

Abstract: Bone formation is a highly energy-demanding process that can be impacted by metabolic disorders. Glucose has been considered the principal substrate for osteoblasts, although fatty acids are also important for osteoblast function. Here, we report that ... ...

Abstract	Bone formation is a highly energy-demanding process that can be impacted by metabolic disorders. Glucose has been considered the principal substrate for osteoblasts, although fatty acids are also important for osteoblast function. Here, we report that osteoblasts can derive energy from endogenous fatty acids stored in lipid droplets via lipolysis and that this process is critical for bone formation. As such, we demonstrate that osteoblasts accumulate lipid droplets that are highly dynamic and provide the molecular mechanism by which they serve as a fuel source for energy generation during osteoblast maturation. Inhibiting cytoplasmic lipolysis leads to both an increase in lipid droplet size in osteoblasts and an impairment in osteoblast function. The fatty acids released by lipolysis from these lipid droplets become critical for cellular energy production as cellular energetics shifts towards oxidative phosphorylation during nutrient-depleted conditions. In vivo, conditional deletion of the ATGL-encoding gene Pnpla2 in osteoblast progenitor cells reduces cortical and trabecular bone parameters and alters skeletal lipid metabolism. Collectively, our data demonstrate that osteoblasts store fatty acids in the form of lipid droplets, which are released via lipolysis to support cellular bioenergetic status when nutrients are limited. Perturbations in this process result in impairment of bone formation, specifically reducing ATP production and overall osteoblast function.
MeSH term(s)	Lipolysis/genetics ; Fatty Acids/metabolism ; Osteogenesis/genetics ; Energy Metabolism ; Osteoblasts/metabolism
Chemical Substances	Fatty Acids
Language	English
Publishing date	2023-11-24
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2803313-9
ISSN	2095-6231 ; 2095-4700
ISSN (online)	2095-6231
ISSN	2095-4700
DOI	10.1038/s41413-023-00297-2
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Article ; Online: Quantitative Lipid Droplet Proteomics Reveals Mycobacterium tuberculosis Induced Alterations in Macrophage Response to Infection.

Menon, Dilip / Singh, Kaurab / Pinto, Sneha M / Nandy, Ananya / Jaisinghani, Neetika / Kutum, Rintu / Dash, Debasis / Prasad, T S Keshava / Gandotra, Sheetal

ACS infectious diseases

2019 Volume 5, Issue 4, Page(s) 559–569

Abstract: Growing evidence suggests the importance of lipid metabolism in pathogenesis of tuberculosis. Neutral lipids form the majority of lipids in a caseous granuloma, a pathology characteristic of tuberculosis. Cytosolic lipid droplets (LDs) of macrophages ... ...

Abstract	Growing evidence suggests the importance of lipid metabolism in pathogenesis of tuberculosis. Neutral lipids form the majority of lipids in a caseous granuloma, a pathology characteristic of tuberculosis. Cytosolic lipid droplets (LDs) of macrophages form the store house of these lipids and have been demonstrated to contribute to the inflammatory response to infection. The proteome of lipid droplets reflects the mechanisms of lipid metabolism active under a condition. However, infection induced changes in the proteome of these dynamic organelles remains elusive. Here, we employed quantitative proteomics to identify alterations induced upon infection with live Mycobacterium tuberculosis (Mtb) in comparison with heat killed bacilli or uninfected macrophages. We found increased abundance of proteins coupled with lipid metabolism, protein synthesis, and vesicular transport function in LDs upon infection with live Mtb. Using biochemical methods and microscopy, we validated ADP-ribosyltransferase (Arf)-like 8 (ARL8B) to be increased on the lipid droplet surface of live Mtb infected macrophages and that ARL8B is a bonafide LD protein. This study provides the first proteomic evidence that the dynamic responses to infection also encompass changes at the level of LDs. This information will be important in understanding how Mtb manipulates lipid metabolism and defense mechanisms of the host macrophage.
MeSH term(s)	Cell Line ; Humans ; Lipid Droplets/chemistry ; Lipid Droplets/metabolism ; Macrophages/chemistry ; Macrophages/immunology ; Macrophages/metabolism ; Macrophages/microbiology ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/physiology ; Proteome/chemistry ; Proteome/genetics ; Proteome/metabolism ; Proteomics ; Tuberculosis/immunology ; Tuberculosis/metabolism ; Tuberculosis/microbiology
Chemical Substances	Proteome
Language	English
Publishing date	2019-02-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2373-8227
ISSN (online)	2373-8227
DOI	10.1021/acsinfecdis.8b00301
Database	MEDical Literature Analysis and Retrieval System OnLINE

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