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  1. Article: Curbing rampant cross-contamination and misidentification of cell lines.

    Nardone, Roland M

    BioTechniques

    2008  Volume 45, Issue 3, Page(s) 221–227

    Abstract: A son's challenge started an emeritus professor of biology on a three-year odyssey to get biological researchers to correct a decades-long problem with cross-contaminated and misidentified cell lines. These errors may account for more than 15% of ... ...

    Abstract A son's challenge started an emeritus professor of biology on a three-year odyssey to get biological researchers to correct a decades-long problem with cross-contaminated and misidentified cell lines. These errors may account for more than 15% of mammalian cultures, wasting resources and undermining the integrity of research.
    MeSH term(s) Animals ; Cell Culture Techniques/methods ; Cell Culture Techniques/standards ; Cell Line ; Cell Lineage ; Cells, Cultured ; DNA Fingerprinting/economics ; DNA Fingerprinting/methods ; DNA, Neoplasm/analysis ; Gene Expression Profiling ; HeLa Cells ; Humans ; Isoenzymes/analysis ; Karyotyping ; Reproducibility of Results ; Tumor Cells, Cultured
    Chemical Substances DNA, Neoplasm ; Isoenzymes
    Language English
    Publishing date 2008-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 48453-2
    ISSN 1940-9818 ; 0736-6205
    ISSN (online) 1940-9818
    ISSN 0736-6205
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Eradication of cross-contaminated cell lines: a call for action.

    Nardone, Roland M

    Cell biology and toxicology

    2007  Volume 23, Issue 6, Page(s) 367–372

    Abstract: This "white paper" was prepared and widely disseminated in an attempt to sound an alarm about the long-term existence of a grave, unresolved and growing problem that affects a significant portion of biomedical research, namely, the use of misidentified ... ...

    Abstract This "white paper" was prepared and widely disseminated in an attempt to sound an alarm about the long-term existence of a grave, unresolved and growing problem that affects a significant portion of biomedical research, namely, the use of misidentified and cross-contaminated cell cultures. The "white paper" shows how bold action could bring about a profession-wide change in practice that would prevent further erosion. Misidentification and inter- and intra-specific cross-contamination of mammalian cell cultures used in research continues as a widespread problem despite an awareness that dates back more than 45 years. Awareness of the problem has led to a good understanding of the causes of cross-contamination and appropriate preventive measures. It has also led to the application of robust methods for the authentication of cell lines. Yet the problem continues unabated. Estimates of the incidence of research papers flawed by the use of misidentified and cross-contaminated cell cultures approximate 15-20%. The gravity of the situation calls for a strategy that would deliver a remedial message of authentication to virtually all cell culture researchers and also ensure compliance with the message. At the core of the strategy proposed herein is having cell line authentication as a condition for the award of research grants and for the publication of research findings.
    MeSH term(s) Animals ; Cell Culture Techniques ; Cell Line ; Humans
    Language English
    Publishing date 2007-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 48824-0
    ISSN 1573-6822 ; 0742-2091
    ISSN (online) 1573-6822
    ISSN 0742-2091
    DOI 10.1007/s10565-007-9019-9
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  3. Article ; Online: Cancer: Authenticate new xenograft models.

    Nardone, Roland M / MacLeod, Roderick A F / Capes-Davis, Amanda

    Nature

    2016  Volume 532, Issue 7599, Page(s) 313

    MeSH term(s) Animals ; Cell Line, Tumor ; DNA Contamination ; Databases, Factual ; Disease Models, Animal ; Guidelines as Topic ; Heterografts/standards ; Humans ; National Cancer Institute (U.S.) ; Neoplasms/pathology ; Quality Control ; Reproducibility of Results ; United States ; Xenograft Model Antitumor Assays/standards
    Language English
    Publishing date 2016-04-25
    Publishing country England
    Document type Letter
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/532313a
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  4. Article: The nexus of biology and the abortion issue.

    Nardone, Roland M

    Jurist (Washington, D.C.)

    1973  Volume 33, Issue 2, Page(s) 153–161

    MeSH term(s) Abortion, Induced ; Beginning of Human Life ; Embryonic and Fetal Development ; Fetus ; Humans ; Individuality ; Life ; Personhood
    Language English
    Publishing date 1973
    Publishing country United States
    Document type Journal Article
    ISSN 0022-6858
    ISSN 0022-6858
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  5. Book: Special tissue culture methods

    Nardone, Roland M

    (Virology)

    1984  

    Institution American Society for Microbiology. / Committee on Educational Materials
    Author's details author, Roland M. Nardone ; produced by the American Society for Microbiology, Committee on Educational Materials
    Series title Virology
    MeSH term(s) Culture Techniques/methods
    Language English
    Size 80 slides :, col. +
    Publisher The Society
    Publishing place Washington, D.C
    Document type Book
    Note Supported by a grant from Corning Glass Works. ; Sound accompaniment compatible for manual and automatic operation. ; Includes guide (16 p.) and post-test. ; Approved for 4 credit hrs. in category 1 applicable also to recertification programs of the National Registry of Microbiologists and the American Board of Medical Microbiology.
    Accompanying material 1 sound cassette (30 min. : 1 7/8 ips) + 1 guide.
    Database Catalogue of the US National Library of Medicine (NLM)

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  6. Book ; Conference proceedings: Mendel centenary

    Nardone, Roland M

    genetics, development and evolution; proceedings of a symposium held at the Catholic University of America, November 3, 1965

    1968  

    Institution Catholic University of America. / Dept. of Biology
    Keywords Evolution. ; Genetics.
    Language English
    Size 174 p., illus.
    Publisher Catholic University of America Press
    Publishing place Washington
    Document type Book ; Conference proceedings
    Database NAL-Catalogue (AGRICOLA)

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  7. Book: Mendel centenary

    Nardone, Roland M

    genetics, development and evolution

    1968  

    Institution Catholic University of America / Dept. of Biology
    Author's details Edited by Roland M. Nardone
    Keywords Evolution (Biology) ; Genetics
    Language English
    Size v, 174 p, illus, 24 cm
    Publisher Catholic University of America Press
    Publishing place Washington
    Document type Book
    Note Includes bibliographies ; Sponsored by the Dept. of Biology of the Catholic University of America
    Database Leibniz Institute of Plant Genetics and Crop Plant Research

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  8. Book ; Conference proceedings: Mendel centenary - genetics, development and evolution;

    Nardone, Roland M

    proceedings of a symposium held at the Catholic University of America, November 3, 1965. Edited by Roland M. Nardone

    1968  

    Title variant Genetics, development and evolution
    Institution Catholic University of America. / Department of Biology
    MeSH term(s) Biological Evolution ; Genetics
    Language English
    Size v, 174 p., illus.
    Publisher Catholic Univ. of America Press
    Publishing place Washington
    Document type Book ; Conference proceedings
    Note Sponsored by the Dept. of Biology of the Catholic University of America.
    Database Catalogue of the US National Library of Medicine (NLM)

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  9. Article ; Online: Match criteria for human cell line authentication: where do we draw the line?

    Capes-Davis, Amanda / Reid, Yvonne A / Kline, Margaret C / Storts, Douglas R / Strauss, Ethan / Dirks, Wilhelm G / Drexler, Hans G / MacLeod, Roderick A F / Sykes, Gregory / Kohara, Arihiro / Nakamura, Yukio / Elmore, Eugene / Nims, Raymond W / Alston-Roberts, Christine / Barallon, Rita / Los, Georgyi V / Nardone, Roland M / Price, Paul J / Steuer, Anton /
    Thomson, Jim / Masters, John R W / Kerrigan, Liz

    International journal of cancer

    2013  Volume 132, Issue 11, Page(s) 2510–2519

    Abstract: Continuous human cell lines have been used extensively as models for biomedical research. In working with these cell lines, researchers are often unaware of the risk of cross-contamination and other causes of misidentification. To reduce this risk, there ...

    Abstract Continuous human cell lines have been used extensively as models for biomedical research. In working with these cell lines, researchers are often unaware of the risk of cross-contamination and other causes of misidentification. To reduce this risk, there is a pressing need to authenticate cell lines, comparing the sample handled in the laboratory to a previously tested sample. The American Type Culture Collection Standards Development Organization Workgroup ASN-0002 has developed a Standard for human cell line authentication, recommending short tandem repeat (STR) profiling for authentication of human cell lines. However, there are known limitations to the technique when applied to cultured samples, including possible genetic drift with passage. In our study, a dataset of 2,279 STR profiles from four cell banks was used to assess the effectiveness of the match criteria recommended within the Standard. Of these 2,279 STR profiles, 1,157 were grouped into sets of related cell lines-duplicate holdings, legitimately related samples or misidentified cell lines. Eight core STR loci plus amelogenin were used to unequivocally authenticate 98% of these related sets. Two simple match algorithms each clearly discriminated between related and unrelated samples, with separation between related samples at ≥80% match and unrelated samples at <50% match. A small degree of overlap was noted at 50-79% match, mostly from cell lines known to display variable STR profiles. These match criteria are recommended as a simple and effective way to interpret results from STR profiling of human cell lines.
    MeSH term(s) Algorithms ; Cell Line ; Gene Expression Profiling/methods ; Genotyping Techniques/standards ; Humans ; Microsatellite Repeats/genetics ; Polymerase Chain Reaction
    Language English
    Publishing date 2013-06-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 218257-9
    ISSN 1097-0215 ; 0020-7136
    ISSN (online) 1097-0215
    ISSN 0020-7136
    DOI 10.1002/ijc.27931
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    Zs.A 478: Show issues Location:
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    Zs.MO 576: Show issues

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  10. Article: Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues

    Barallon, Rita / Bauer, Steven R / Butler, John / Capes-Davis, Amanda / Dirks, Wilhelm G / Elmore, Eugene / Furtado, Manohar / Kline, Margaret C / Kohara, Arihiro / Los, Georgyi V / MacLeod, Roderick A. F / Masters, John R. W / Nardone, Mark / Nardone, Roland M / Nims, Raymond W / Price, Paul J / Reid, Yvonne A / Shewale, Jaiprakash / Sykes, Gregory /
    Steuer, Anton F / Storts, Douglas R / Thomson, Jim / Taraporewala, Zenobia / Alston-Roberts, Christine / Kerrigan, Liz

    In vitro cellular & developmental biology. Animal. 2010 Oct., v. 46, no. 9

    2010  

    Abstract: Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising ... ...

    Abstract Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues.
    Language English
    Dates of publication 2010-10
    Size p. 727-732.
    Publisher Springer-Verlag
    Publishing place New York
    Document type Article
    ISSN 1071-2690
    DOI 10.1007/s11626-010-9333-z
    Database NAL-Catalogue (AGRICOLA)

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