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  1. Article: MYC integrates FSH signalling networks in cumulus cells during bovine oocyte maturation

    Cantanhêde, Ludymila F. / Moura, Marcelo T. / Oliveira-Silva, Roberta L. / Nascimento, Pábola S. / Ferreira-Silva, José C. / Benko-Iseppon, Ana M. / Oliveira, Marcos A. L.

    Acta veterinaria Hungarica. 2022 May 02,

    2022  

    Abstract: Follicle-stimulating hormone (FSH) contributes to the acquisition of oocyte competence by modulating signalling pathways in cumulus cells (CCs), albeit much less is known about transcription factors (TFs) that orchestrate the downstream transcriptional ... ...

    Abstract Follicle-stimulating hormone (FSH) contributes to the acquisition of oocyte competence by modulating signalling pathways in cumulus cells (CCs), albeit much less is known about transcription factors (TFs) that orchestrate the downstream transcriptional changes. This work allowed to prospect TFs involved in FSH-mediated signalling during oocyte in vitro maturation (IVM). Bovine cumulus-oocyte complexes underwent IVM with FSH (FSH+) or without FSH (control/CTL) for 22 h, and CCs were subjected to gene expression profiling. Five software identified reference genes for RT-qPCR (ATP1A1, UBB, and YWHAZ). The transcript levels of FSH-responsive genes HAS2 and PTGS2 (COX2) validated the experimental design. Among candidate TFs, MYC was down-regulated (0.35-fold; P < 0.0001), and THAP11 (RONIN) was up-regulated (1.47-fold; P = 0.016) under FSH+ conditions. In silico analyses predicted binding motifs at MYC and THAP11 genes for previously known FSH-responsive TFs. Signalling pathways (EGFR, ERK, GSK3, PKA, and P38) may execute post-translational regulation due to potential phosphorylation sites in MYC and THAP11 proteins. Prediction of protein–protein interaction networks showed MYC as a core component of FSH signalling, albeit THAP11 acts independently. Hence, MYC integrates FSH signalling networks and may assist in exploring genome-wide transcriptional changes associated with the acquisition of oocyte competence.
    Keywords cattle ; computer simulation ; computer software ; experimental design ; follicle-stimulating hormone ; gene expression ; oocytes ; phosphorylation ; prediction ; protein-protein interactions ; transcription (genetics)
    Language English
    Dates of publication 2022-0502
    Publishing place Akadémiai Kiadó
    Document type Article
    ZDB-ID 605742-1
    ISSN 1588-2705 ; 0365-8198 ; 0236-6290
    ISSN (online) 1588-2705
    ISSN 0365-8198 ; 0236-6290
    DOI 10.1556/004.2022.00007
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 483: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Pluripotency transcription factor levels in sheep embryos correlate with mRNA regulatory elements

    Nascimento, Pábola S. / Moura, Marcelo T. / Oliveira-Silva, Roberta L. / Ramos-Deus, Pamela / Ferreira-Silva, José C. / Filho, Antônio S. Santos / Bartolomeu, Cláudio C. / Benko-Iseppon, Ana M. / Oliveira, Marcos A.L.

    Livestock science. 2022 Jan., v. 255

    2022  

    Abstract: Pluripotency-associated transcription factors (PATF) contribute to cell fate decisions in embryos, cellular reprogramming, and the derivation of stem cells. PATF hold species-specific mRNA fluctuations during early development but the regulatory ... ...

    Abstract Pluripotency-associated transcription factors (PATF) contribute to cell fate decisions in embryos, cellular reprogramming, and the derivation of stem cells. PATF hold species-specific mRNA fluctuations during early development but the regulatory determinants remain largely unknown in sheep. The aim of this study was to prospect correlations between PATF gene expression patterns and mRNA regulatory features. The relative expression of PATF was investigated in sheep embryos by RT-qPCR and mRNAs were investigated for cis-regulatory elements and N6-methyladenosine (m6A) RNA methylation using bioinformatics. Four PATF gene expression patterns emerged with relatively distinct mRNA regulatory modes. RXRβ and TCF3 were detected in oocytes and mRNAs had variable 3′untranslated region (3′UTR) length. Three mRNAs (DPPA3, THAP11, and ZFX), which had similar abundance among developmental stages, frequently had short 3′UTRs and polyadelylation sites. NR5A2 and SP1 levels increased at the cleavage-stage but diminished in morulae, thus showing long 3′UTRs with multiple polyadelylation and m6A sites. Finally, mRNA abundance was low in oocytes and up-regulated in morulae (UTF1 and ZNF281) without a distinctive regulatory mode. Hence, sheep embryos hold PATF gene expression that correlates with mRNA 3′UTR length and the number of polyadenylation and m6A sites. These findings may lead to modulating sheep PATF to enhance reprogramming and stem cell derivation.
    Keywords bioinformatics ; early development ; gene expression ; methylation ; oocytes ; sheep ; stem cells ; transcription factors
    Language English
    Dates of publication 2022-01
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2226176-X
    ISSN 1878-0490 ; 1871-1413
    ISSN (online) 1878-0490
    ISSN 1871-1413
    DOI 10.1016/j.livsci.2021.104778
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3115: Show issues

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  3. Article: MYC integrates FSH signalling networks in cumulus cells during bovine oocyte maturation.

    Cantanhêde, Ludymila F / Moura, Marcelo T / Oliveira-Silva, Roberta L / Nascimento, Pábola S / Ferreira-Silva, José C / Benko-Iseppon, Ana M / Oliveira, Marcos A L

    Acta veterinaria Hungarica

    2022  

    Abstract: Follicle-stimulating hormone (FSH) contributes to the acquisition of oocyte competence by modulating signalling pathways in cumulus cells (CCs), albeit much less is known about transcription factors (TFs) that orchestrate the downstream transcriptional ... ...

    Abstract Follicle-stimulating hormone (FSH) contributes to the acquisition of oocyte competence by modulating signalling pathways in cumulus cells (CCs), albeit much less is known about transcription factors (TFs) that orchestrate the downstream transcriptional changes. This work allowed to prospect TFs involved in FSH-mediated signalling during oocyte in vitro maturation (IVM). Bovine cumulus-oocyte complexes underwent IVM with FSH (FSH+) or without FSH (control/CTL) for 22 h, and CCs were subjected to gene expression profiling. Five software identified reference genes for RT-qPCR (ATP1A1, UBB, and YWHAZ). The transcript levels of FSH-responsive genes HAS2 and PTGS2 (COX2) validated the experimental design. Among candidate TFs, MYC was down-regulated (0.35-fold; P < 0.0001), and THAP11 (RONIN) was up-regulated (1.47-fold; P = 0.016) under FSH+ conditions. In silico analyses predicted binding motifs at MYC and THAP11 genes for previously known FSH-responsive TFs. Signalling pathways (EGFR, ERK, GSK3, PKA, and P38) may execute post-translational regulation due to potential phosphorylation sites in MYC and THAP11 proteins. Prediction of protein-protein interaction networks showed MYC as a core component of FSH signalling, albeit THAP11 acts independently. Hence, MYC integrates FSH signalling networks and may assist in exploring genome-wide transcriptional changes associated with the acquisition of oocyte competence.
    Language English
    Publishing date 2022-05-02
    Publishing country Hungary
    Document type Journal Article
    ZDB-ID 605742-1
    ISSN 1588-2705 ; 0236-6290 ; 0365-8198
    ISSN (online) 1588-2705
    ISSN 0236-6290 ; 0365-8198
    DOI 10.1556/004.2022.00007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 483: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Evolutionary-driven C-MYC gene expression in mammalian fibroblasts.

    Moura, Marcelo T / Silva, Roberta L O / Cantanhêde, Ludymila F / Ferreira-Silva, José C / Nascimento, Pábola S / Benko-Iseppon, Ana M / Oliveira, Marcos A L

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 11056

    Abstract: The extent to which mammalian cells share similar transcriptomes remains unclear. Notwithstanding, such cross-species gene expression inquiries have been scarce for defined cell types and most lack the dissection of gene regulatory landscapes. Therefore, ...

    Abstract The extent to which mammalian cells share similar transcriptomes remains unclear. Notwithstanding, such cross-species gene expression inquiries have been scarce for defined cell types and most lack the dissection of gene regulatory landscapes. Therefore, the work was aimed to determine C-MYC relative expression across mammalian fibroblasts (Ovis aries and Bos taurus) via cross-species RT-qPCR and comprehensively explore its regulatory landscape by in silico tools. The prediction of transcription factor binding sites in C-MYC and its 2.5 kb upstream sequence revealed substantial variation, thus indicating evolutionary-driven re-wiring of cis-regulatory elements. C-MYC and its downstream target TBX3 were up-regulated in Bos taurus fibroblasts. The relative expression of C-MYC regulators [RONIN (also known as THAP11), RXRβ, and TCF3] and the C-MYC-associated transcript elongation factor CDK9 did not differ between species. Additional in silico analyses suggested Bos taurus-specific C-MYC exonization, alternative splicing, and binding sites for non-coding RNAs. C-MYC protein orthologs were highly conserved, while variation was in the transactivation domain and the leucine zipper motif. Altogether, mammalian fibroblasts display evolutionary-driven C-MYC relative expression that should be instructive for understanding cellular physiology, cellular reprogramming, and C-MYC-related diseases.
    MeSH term(s) Amino Acid Sequence ; Animals ; Cattle/genetics ; Cattle/metabolism ; Cyclin-Dependent Kinase 9/genetics ; Evolution, Molecular ; Fibroblasts/metabolism ; Gene Expression ; Genes, myc ; Protein Processing, Post-Translational ; Proto-Oncogene Proteins c-myc/genetics ; Proto-Oncogene Proteins c-myc/metabolism ; Regulatory Elements, Transcriptional ; Sequence Homology, Amino Acid ; Sheep, Domestic/genetics ; Sheep, Domestic/metabolism ; Species Specificity ; T-Box Domain Proteins/genetics ; Transcriptome
    Chemical Substances Proto-Oncogene Proteins c-myc ; T-Box Domain Proteins ; Cyclin-Dependent Kinase 9 (EC 2.7.11.22)
    Language English
    Publishing date 2020-07-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-67391-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set.

    Moura, Marcelo T / Silva, Roberta L O / Nascimento, Pábola S / Ferreira-Silva, José C / Cantanhêde, Ludymila F / Kido, Ederson A / Benko-Iseppon, Ana M / Oliveira, Marcos A L

    PloS one

    2019  Volume 14, Issue 8, Page(s) e0221170

    Abstract: Quantitative reverse transcription PCR (RT-qPCR) remains as an accurate approach for gene expression analysis but requires labor-intensive validation of reference genes using species-specific primers. To ease such demand, the aim was to design and test a ...

    Abstract Quantitative reverse transcription PCR (RT-qPCR) remains as an accurate approach for gene expression analysis but requires labor-intensive validation of reference genes using species-specific primers. To ease such demand, the aim was to design and test a multi-species primer set to validate reference genes for inter-genus RT-qPCR gene expression analysis. Primers were designed for ten housekeeping genes using transcript sequences of various livestock species. All ten gene transcripts were detected by RT-PCR in Bos taurus (cattle), Bubalus bubalis (buffaloes), Capra hircus (goats), and Ovis aries (sheep) cDNA. Primer efficiency was attained for eight reference genes using B. taurus-O. aries fibroblast cDNA (95.54-98.39%). The RT-qPCR data normalization was carried out for B. taurus vs. O. aries relative gene expression using Bestkeeper, GeNorm, Norm-finder, Delta CT method, and RefFinder algorithms. Validation of inter-genus RT-qPCR showed up-regulation of TLR4 and ZFX gene transcripts in B. taurus fibroblasts, irrespectively of normalization conditions (two, three, or four reference genes). In silico search in mammalian transcriptomes showed that the multi-species primer set is expected to amplify transcripts of at least two distinct loci in 114 species, and 79 species would be covered by six or more primers. Hence, a multi-species primer set allows for inter-genus gene expression analysis between O. aries and B. taurus fibroblasts and further reveals species-specific gene transcript abundance of key transcription factors.
    MeSH term(s) Animals ; Buffaloes ; Cattle ; DNA Primers/genetics ; Fibroblasts/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Genetic Loci ; Goats ; Livestock/genetics ; Livestock/metabolism ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Sheep ; Species Specificity ; Transcriptome
    Chemical Substances DNA Primers
    Language English
    Publishing date 2019-08-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0221170
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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