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  1. Article: Follicular size, luteinizing hormone (LH), and progesterone (P4) levels in postpartum Santa Inês ewes subjected to ram effect combined with suckling interruption

    Ferreira-Silva, José Carlos / Filho, Fernando Tenório / Moura, Marcelo Tigre / Nascimento, Pábola Santos / Oliveira, Luis Rennan Sampaio / Bartolomeu, Claudio Coutinho / Oliveira, Marcos Antonio Lemos

    Livestock science. 2018 Aug., v. 214

    2018  

    Abstract: The objective was to evaluate the role of ram effect combined with temporary weaning on P4, LH, follicular size and reproductive performance of hair ewes during postpartum. Pluriparous Santa Inês ewes (n = 99) during 15 to 30 days postpartum were ... ...

    Abstract The objective was to evaluate the role of ram effect combined with temporary weaning on P4, LH, follicular size and reproductive performance of hair ewes during postpartum. Pluriparous Santa Inês ewes (n = 99) during 15 to 30 days postpartum were selected and isolated from rams for an additional period of 30 days. Before exposure to rams, ewes were allocated to experimental groups, where on SI0, no suckling interruption was performed, and in SI24 and SI48, suckling interruption was performed for 24 and 48 hours, respectively. Mean P4 was according to anestrus condition before the introduction of rams, and it reached cyclicity levels after contact between genders. Ewes displaying a single estrus were more frequent in SI24 than SI48 (P 〈 0.05). The LH pre-ovulatory peaks were detected within 44 hours and 60 hours after introduction of rams, irrespectively of the group. Ovulatory follicles reached a more significant size in SI24 than SI0 and SI48 (P < 0.05). Pregnancy rates differed at first service since SI24 was higher than SI48. Moreover, prolificacy was similar among groups (P 〉 0.05). Ram effect combined with temporary weaning for 24 hours is more efficient for Santa Inês reproduction, since it leads to larger ovulatory follicle size, increased the P4 level and higher pregnancy rate at first service.
    Keywords anestrus ; estrus ; ewes ; luteinization ; luteinizing hormone ; periodicity ; pregnancy rate ; progesterone ; ram effect ; rams ; reproductive performance ; suckling ; weaning
    Language English
    Dates of publication 2018-08
    Size p. 88-92.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2226176-X
    ISSN 1878-0490 ; 1871-1413
    ISSN (online) 1878-0490
    ISSN 1871-1413
    DOI 10.1016/j.livsci.2018.05.016
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Housekeeping genes for RT-qPCR in ovine preimplantation embryos.

    Nascimento, Pábola Santos / Moura, Marcelo Tigre / Silva, Roberta Lane Oliveira / Ramos-Deus, Pamela / Ferreira-Silva, José Carlos / Veira, Joane Isis Travassos / Santos Filho, Antônio Santana / Guido, Sebastião Inocêncio / Bartolomeu, Cláudio Coutinho / Benko-Iseppon, Ana Maria / Oliveira, Marcos Antonio Lemos

    Zygote (Cambridge, England)

    2020  , Page(s) 1–8

    Abstract: Housekeeping genes (HKG) are paramount for accurate gene expression analysis during preimplantation development. Markedly, quantitative reverse transcription polymerase chain reaction (RT-qPCR) in ovine embryos currently lacks HKGs. Therefore, we tested ... ...

    Abstract Housekeeping genes (HKG) are paramount for accurate gene expression analysis during preimplantation development. Markedly, quantitative reverse transcription polymerase chain reaction (RT-qPCR) in ovine embryos currently lacks HKGs. Therefore, we tested 11 HKGs for RT-qPCR normalization during ovine parthenogenetic preimplantation development. Seven HKGs reached the qPCR efficiency threshold (97.20-105.96%), with correlation coefficients ranging from -0.922 to -0.998 and slopes from -3.22 to -3.59. GeNorm ranked glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) as the best HKG pair, while H3 histone, family 3A (H3F3A) was the third HKG. Relative gene expression was measured for zinc finger protein X-linked (ZFX) and developmental pluripotency-associated 3 (DPPA3) transcripts during ovine parthenogenetic preimplantation development. ZFX did not show any transcript abundance fluctuation among oocytes, cleavage-stage embryos, and morulae. DPPA3 transcript abundance was also similar among all developmental stages, therefore suggesting that it may not display a maternal gene expression profile. In silico analysis of ovine DPPA3 mRNA and protein showed high conservation to bovine orthologues. However, DPPA3 orthologues differed in regulatory motifs. In conclusion, GAPDH, TBP and H3F3A are stable HKGs in ovine parthenogenetic embryos and allow accurate RT-qPCR-based gene expression analysis.
    Language English
    Publishing date 2020-07-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 1166294-3
    ISSN 1469-8730 ; 0967-1994
    ISSN (online) 1469-8730
    ISSN 0967-1994
    DOI 10.1017/S0967199420000295
    Database MEDical Literature Analysis and Retrieval System OnLINE

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