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  1. Article ; Online: Understanding the influence of experimental factors on bio-interactions of nanoparticles: Towards improving correlation between in vitro and in vivo studies.

    Natarajan, Pavithra / Tomich, John M

    Archives of biochemistry and biophysics

    2020  Volume 694, Page(s) 108592

    Abstract: Bionanotechnology has developed rapidly over the past two decades, owing to the extensive and versatile, functionalities and applicability of nanoparticles (NPs). Fifty-one nanomedicines have been approved by FDA since 1995, out of the many NPs based ... ...

    Abstract Bionanotechnology has developed rapidly over the past two decades, owing to the extensive and versatile, functionalities and applicability of nanoparticles (NPs). Fifty-one nanomedicines have been approved by FDA since 1995, out of the many NPs based formulations developed to date. The general conformation of NPs consists of a core with ligands coating their surface, that stabilizes them and provides them with added functionalities. The physicochemical properties, especially the surface composition of NPs influence their bio-interactions to a large extent. This review discusses recent studies that help understand the nano-bio interactions of iron oxide and gold NPs with different surface compositions. We discuss the influence of the experimental factors on the outcome of the studies and, thus, the importance of standardization in the field of nanotechnology. Recent studies suggest that with careful selection of experimental parameters, it is possible to improve the positive correlation between in vitro and in vivo studies. This provides a fundamental understanding of the NPs which helps in assessing their potential toxic side effects and may aid in manipulating them further to improve their biocompatibility and biosafety.
    MeSH term(s) Animals ; Cell Line, Tumor ; Endocytosis ; Gold/chemistry ; Humans ; Immunity/drug effects ; Ligands ; Magnetic Iron Oxide Nanoparticles/chemistry ; Magnetic Iron Oxide Nanoparticles/toxicity ; Nanotechnology/standards ; Reactive Oxygen Species/metabolism ; Surface Properties
    Chemical Substances Ligands ; Reactive Oxygen Species ; Gold (7440-57-5)
    Keywords covid19
    Language English
    Publishing date 2020-09-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 523-x
    ISSN 1096-0384 ; 0003-9861
    ISSN (online) 1096-0384
    ISSN 0003-9861
    DOI 10.1016/j.abb.2020.108592
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    In stock of ZB MED Cologne/Königswinter

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  2. Article: Understanding the influence of experimental factors on bio-interactions of nanoparticles: Towards improving correlation between in vitro and in vivo studies

    Natarajan, Pavithra / Tomich, John M

    Archives of biochemistry and biophysics. 2020 Nov. 15, v. 694

    2020  

    Abstract: Bionanotechnology has developed rapidly over the past two decades, owing to the extensive and versatile, functionalities and applicability of nanoparticles (NPs). Fifty-one nanomedicines have been approved by FDA since 1995, out of the many NPs based ... ...

    Abstract Bionanotechnology has developed rapidly over the past two decades, owing to the extensive and versatile, functionalities and applicability of nanoparticles (NPs). Fifty-one nanomedicines have been approved by FDA since 1995, out of the many NPs based formulations developed to date. The general conformation of NPs consists of a core with ligands coating their surface, that stabilizes them and provides them with added functionalities. The physicochemical properties, especially the surface composition of NPs influence their bio-interactions to a large extent. This review discusses recent studies that help understand the nano-bio interactions of iron oxide and gold NPs with different surface compositions. We discuss the influence of the experimental factors on the outcome of the studies and, thus, the importance of standardization in the field of nanotechnology. Recent studies suggest that with careful selection of experimental parameters, it is possible to improve the positive correlation between in vitro and in vivo studies. This provides a fundamental understanding of the NPs which helps in assessing their potential toxic side effects and may aid in manipulating them further to improve their biocompatibility and biosafety.
    Keywords biocompatibility ; biophysics ; biosafety ; iron oxides ; ligands ; nanobiotechnology ; nanogold ; nanomedicine ; toxicity
    Language English
    Dates of publication 2020-1115
    Publishing place Elsevier Inc.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 523-x
    ISSN 1096-0384 ; 0003-9861
    ISSN (online) 1096-0384
    ISSN 0003-9861
    DOI 10.1016/j.abb.2020.108592
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  3. Article ; Online: Biodistribution Analysis of Peptide-Coated Magnetic Iron Nanoparticles: A Simple and Quantitative Method.

    Natarajan, Pavithra / Horak, Katherine / Rowe, Jennifer / Yoon, Sungmin / Lingo, Joshua / Tomich, John M / Fleming, Sherry D

    Molecular pharmaceutics

    2024  Volume 21, Issue 2, Page(s) 970–981

    Abstract: Biodistribution tracks compounds or molecules of interest in vivo to understand a compound's anticipated efficacy and safety. Nanoparticles deliver nucleic acid and drug payloads and enhance tumor permeability due to multiple properties such as high ... ...

    Abstract Biodistribution tracks compounds or molecules of interest in vivo to understand a compound's anticipated efficacy and safety. Nanoparticles deliver nucleic acid and drug payloads and enhance tumor permeability due to multiple properties such as high surface area to volume ratio, surface functionalization, and modifications. Studying the in vivo biodistribution of nanoparticles documents the effectiveness and safety of nanoparticles and facilitates a more application-driven approach for nanoparticle development that allows for more successful translation into clinical use. In this study, we present a relatively simple method to determine the biodistribution of magnetic iron nanoparticles in mice. In vitro, cells take up branched amphiphilic peptide-coated magnetic nanobeads (BAPc-MNBs) like their counterparts, i.e., branched amphiphilic peptide capsules (BAPCs) with a hollow water-filled core. Both BAPc-MNBs and BAPCs have widespread applications as a nanodelivery system. We evaluated the BAPc-MNBs tissue distribution in wild-type mice injected intravenously (i.v.), intraperitoneally (i.p.), or orally gavaged to understand the biological interactions and to further the development of branched amphiphilic peptide-based nanoparticles. The magnetic nanoparticles allowed collection of the BAPc-MNBs from multiple organs by magnetic bead sorting, followed by a high-throughput screening for iron content. When injected i.v., nanoparticles were distributed widely to various organs before elimination from the system via the intestines in feces. The spleen accumulated the highest amount of BAPc-MNBs in mice administered NPs via i.v. and i.p. but not via oral gavage. Taken together, these data demonstrate that the magnetic sorting not only allowed quantification of the BAPc-MNBs but also identified the distribution of BAPc-MNBs after distinct administration methods.
    MeSH term(s) Mice ; Animals ; Tissue Distribution ; Peptides/chemistry ; Nanoparticles/chemistry ; Magnetic Iron Oxide Nanoparticles ; Magnetite Nanoparticles/chemistry ; Benzenesulfonates
    Chemical Substances methyl-4-nitrobenzenesulfonate (6214-20-6) ; Peptides ; Magnetite Nanoparticles ; Benzenesulfonates
    Language English
    Publishing date 2024-01-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2138405-8
    ISSN 1543-8392 ; 1543-8384
    ISSN (online) 1543-8392
    ISSN 1543-8384
    DOI 10.1021/acs.molpharmaceut.3c01080
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6250: Show issues Location:
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  4. Article: Biodistribution Analysis of Peptide-Coated Magnetic Iron Nanoparticles: A Simple and Quantitative Method.

    Natarajan, Pavithra / Horak, Katherine / Rowe, Jennifer / Lingo, Joshua / Tomich, John M / Fleming, Sherry D

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Biodistribution is the tracking of compounds or molecules of interest in the subject which is integral to understanding their anticipated efficacy and safety. Nanoparticles are highly desirable delivery systems which have the ability to deliver higher ... ...

    Abstract Biodistribution is the tracking of compounds or molecules of interest in the subject which is integral to understanding their anticipated efficacy and safety. Nanoparticles are highly desirable delivery systems which have the ability to deliver higher nucleic acid and drug payloads and they have enhanced tumor permeability due to their unique properties such as high surface area to volume ratio. Studying the biodistribution of nanoparticles is crucial to understand their effectiveness and safety in vivo, facilitate a more application driven approach for nanoparticle development which will lead to their successful translation into clinical use. In this study, we present a relatively simple method to determine the biodistribution of magnetic iron nanoparticles in mice. Branched Amphiphilic Peptide coated Magnetic Nanobeads BAPc-MNBs like their counterpart i.e., Branched Amphiphilic Peptide capsules (BAPCs) with a hollow water-filled core, are readily taken up by cells in vitro and have widespread application as a nanodelivery systems. We evaluated the BAPc-MNBs tissue distribution in wildtype mice injected intravenously (i.v.), intraperitoneally (i.p.) or orally gavaged to understand the biological interactions of the peptide nanoparticles and to further the development of branched amphiphilic peptides-based nanoparticles. BAPc-MNBs were distributed widely to various organs when injected i.v. and were eliminated from the system via the intestines in feces. The spleen was found to accumulate the highest amount of BAPc-MNBs in mice administered the NPs i.v. and i.p. while they were not absorbed into the system via oral gavage. This study not only presents a relatively simple quantification method to determine in vivo biodistribution of magnetic iron nanoparticles that can be widely applied but also demonstrates the potential of Branched Amphiphilic Peptides in the form of BAPCs or BAPc-MNBs as a delivery system.
    Language English
    Publishing date 2023-10-11
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.10.11.561862
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A case of relapsed visceral Kaposi's sarcoma with bilateral chylothoraces successfully treated with paclitaxel.

    Natarajan, Pavithra / Miller, Alastair

    International journal of STD & AIDS

    2015  Volume 26, Issue 8, Page(s) 605–609

    Abstract: Chylothorax is a rare complication of visceral Kaposi's sarcoma. We report a case with bilateral chylothoraces secondary to relapsed visceral Kaposi's sarcoma who was successfully treated with paclitaxel chemotherapy. ...

    Abstract Chylothorax is a rare complication of visceral Kaposi's sarcoma. We report a case with bilateral chylothoraces secondary to relapsed visceral Kaposi's sarcoma who was successfully treated with paclitaxel chemotherapy.
    MeSH term(s) Adult ; Antineoplastic Agents, Phytogenic/therapeutic use ; Chylothorax/complications ; Chylothorax/diagnosis ; HIV Infections/complications ; HIV Infections/diagnosis ; Humans ; Male ; Paclitaxel/therapeutic use ; Recurrence ; Sarcoma, Kaposi/complications ; Sarcoma, Kaposi/diagnosis ; Sarcoma, Kaposi/drug therapy ; Treatment Outcome
    Chemical Substances Antineoplastic Agents, Phytogenic ; Paclitaxel (P88XT4IS4D)
    Language English
    Publishing date 2015-07
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 1018089-8
    ISSN 1758-1052 ; 0956-4624
    ISSN (online) 1758-1052
    ISSN 0956-4624
    DOI 10.1177/0956462414546915
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2829: Show issues Location:
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  8. Article ; Online: Donor-derived Cryptococcus gattii sensu stricto infection in two kidney transplant recipients, southeastern United States.

    Natarajan, Pavithra / Lockhart, Shawn R / Basavaraju, Sridhar V / Anjan, Shweta / Lindsley, Mark D / McGrath, Martina M / Oh, David H / Jackson, Brendan R

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    2021  Volume 21, Issue 11, Page(s) 3780–3784

    Abstract: Cryptococcus gattii infection is a rare cause of severe pulmonary disease and meningoencephalitis that has only recently been detected in the southeastern United States. We describe an organ transplant-associated outbreak of C. gattii infection involving ...

    Abstract Cryptococcus gattii infection is a rare cause of severe pulmonary disease and meningoencephalitis that has only recently been detected in the southeastern United States. We describe an organ transplant-associated outbreak of C. gattii infection involving an HIV-negative immunosuppressed donor in this region who died following new-onset headache and seizure of unknown cause. Retrospective cryptococcal antigen (CrAg) testing of donor serum was positive. Two of the three transplant recipients developed severe C. gattii infection 11 and 12 weeks following transplantation. One recipient died from severe pulmonary infection, identified on autopsy, and the other ill recipient survived following treatment for cryptococcal meningitis. This outbreak underscores the importance of considering cryptococcosis in patients with clinical findings suggestive of subacute meningitis or other central nervous system (CNS) pathology, and the potential benefit of routine pre-transplant donor CrAg screening using lateral flow assay to guide recipient antifungal prophylaxis. The case also adds to emerging evidence that C. gattii is a potential threat in the southeastern United States.
    MeSH term(s) Cryptococcosis/diagnosis ; Cryptococcosis/drug therapy ; Cryptococcosis/etiology ; Cryptococcus gattii ; Humans ; Kidney Transplantation/adverse effects ; Retrospective Studies ; Southeastern United States/epidemiology ; Tissue Donors ; Transplant Recipients
    Language English
    Publishing date 2021-08-31
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2060594-8
    ISSN 1600-6143 ; 1600-6135
    ISSN (online) 1600-6143
    ISSN 1600-6135
    DOI 10.1111/ajt.16729
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5542: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  9. Article ; Online: A Study of the Cellular Uptake of Magnetic Branched Amphiphilic Peptide Capsules.

    Natarajan, Pavithra / Roberts, Jonathan D / Kunte, Nitish / Hunter, Wayne B / Fleming, Sherry D / Tomich, John M / Avila, L Adriana

    Molecular pharmaceutics

    2020  Volume 17, Issue 6, Page(s) 2208–2220

    Abstract: Understanding cellular uptake mechanisms of nanoparticles with therapeutic potential has become critical in the field of drug delivery. Elucidation of cellular entry routes can aid in the dissection of the complex intracellular trafficking and ... ...

    Abstract Understanding cellular uptake mechanisms of nanoparticles with therapeutic potential has become critical in the field of drug delivery. Elucidation of cellular entry routes can aid in the dissection of the complex intracellular trafficking and potentially allow for the manipulation of nanoparticle fate after cellular delivery (i.e., avoid lysosomal degradation). Branched amphiphilic peptide capsules (BAPCs) are peptide nanoparticles that have been and are being explored as delivery systems for nucleic acids and other therapeutic molecules in vitro and in vivo. In the present study, we determined the cellular uptake routes of BAPCs with and without a magnetic nanobead core (BAPc-MNBs) in two cell lines: macrophages and intestinal epithelial cells. We also studied the influence of size and growth media composition in this cellular process. Substituting the water-filled core with magnetic nanobeads might provide the peptide bilayer nanocapsules with added functionalities, facilitating their use in bio/immunoassays, magnetic field guided drug delivery, and magnetofection among others. Results suggest that BAPc-MNBs are internalized into the cytosol using more than one endocytic pathway. Flow cytometry and analysis of reactive oxygen and nitrogen species (ROS/RNS) demonstrated that cell viability was minimally impacted by BAPc-MNBs. Cellular uptake pathways of peptide vesicles remain poorly understood, particularly with respect to endocytosis and intracellular trafficking. Outcomes from these studies provide a fundamental understanding of the cellular uptake of this peptide-based delivery system which will allow for strengthening of their delivery capabilities and expanding their applications both in vitro and in vivo.
    MeSH term(s) Endocytosis/physiology ; Flow Cytometry ; Microscopy, Confocal ; Nanoparticles/chemistry ; Peptides/chemistry ; Reactive Nitrogen Species/metabolism ; Reactive Oxygen Species/metabolism
    Chemical Substances Peptides ; Reactive Nitrogen Species ; Reactive Oxygen Species
    Language English
    Publishing date 2020-05-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2138405-8
    ISSN 1543-8392 ; 1543-8384
    ISSN (online) 1543-8392
    ISSN 1543-8384
    DOI 10.1021/acs.molpharmaceut.0c00393
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  10. Article: Intrinsic Kinetic Study for Photocatalytic Degradation of Diclofenac under UV and Visible Light

    Hashim, Noshin / Natarajan Pavithra / Ray Ajay K

    Industrial & Engineering Chemistry Research. 2014 Dec. 10, v. 53, no. 49

    2014  

    Abstract: This study employs a semibatch, swirl-flow, monolithic type photocatalytic reactor to determine intrinsic kinetic parameters for the photocatalytic degradation of a common anti-inflammatory drug, diclofenac (DCF), in an immobilized system under both UV ... ...

    Abstract This study employs a semibatch, swirl-flow, monolithic type photocatalytic reactor to determine intrinsic kinetic parameters for the photocatalytic degradation of a common anti-inflammatory drug, diclofenac (DCF), in an immobilized system under both UV and visible radiation. The goal of this work was (a) to find a better reactor configuration that provides improved residence time distribution of fluid, (b) to obtain a suitable support system (such as fiberglass sheet) for the immobilization of various photocatalysts (Degussa P25 TiO₂ and modified TiO₂ (doped and dye-sensitized), (c) to compare degradation rates with Degussa P25 when doped and dye-sensitized photocatalysts is used particularly under visible radiation, and finally (d) to determine true kinetic parameters after correcting for external mass transfer resistance that exists when catalysts is immobilized as a function of various operating parameters such as flow rate, catalyst loading, pH, light intensity, initial concentration, and photocatalyst type. The objective of this study lies in obtaining true kinetic data independent of reactor types and, therefore, can be used for process scale-up and high-rate water treatment. It was observed experimentally that a better degradation rate can be achieved with dye-sensitized catalysts activated under visible light.
    Keywords anti-inflammatory agents ; diclofenac ; engineering ; fiberglass ; light intensity ; mass transfer ; pH ; photocatalysis ; photocatalysts ; titanium dioxide ; water treatment
    Language English
    Dates of publication 2014-1210
    Size p. 18637-18646.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1484436-9
    ISSN 1520-5045 ; 0888-5885
    ISSN (online) 1520-5045
    ISSN 0888-5885
    DOI 10.1021%2Fie404355k
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