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  1. Artikel ; Online: Draft Genome Sequences of Two Virulent Streptococcus equi subsp.

    Surendran Nair, Meera / Byukusenge, Maurice / Li, Lingling / Nissly, Ruth H / Cavener, Victoria S / Yon, Michele / Barry, Rhiannon / Natesan, Pazhanivel / Thirumalapura, Nagaraja / Tewari, Deepanker / Jayarao, Bhushan M / Kuchipudi, Suresh V

    Microbiology resource announcements

    2020  Band 9, Heft 42

    Abstract: Draft genome sequences of two outbreak isolates ... ...

    Abstract Draft genome sequences of two outbreak isolates of
    Sprache Englisch
    Erscheinungsdatum 2020-10-15
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00974-20
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Development and Validation of Indirect Enzyme-Linked Immunosorbent Assays for Detecting Antibodies to SARS-CoV-2 in Cattle, Swine, and Chicken

    Gontu, Abhinay / Marlin, Erika A. / Ramasamy, Santhamani / Neerukonda, Sabarinath / Anil, Gayatri / Morgan, Jasmine / Quraishi, Meysoon / Chen, Chen / Boorla, Veda Sheersh / Nissly, Ruth H. / Jakka, Padmaja / Chothe, Shubhada K. / Ravichandran, Abirami / Kodali, Nishitha / Amirthalingam, Saranya / LaBella, Lindsey / Kelly, Kathleen / Natesan, Pazhanivel / Minns, Allen M. /
    Rossi, Randall M. / Werner, Jacob R. / Hovingh, Ernest / Lindner, Scott E. / Tewari, Deepanker / Kapur, Vivek / Vandegrift, Kurt J. / Maranas, Costas D. / Surendran Nair, Meera / Kuchipudi, Suresh V.

    Viruses. 2022 June 22, v. 14, no. 7

    2022  

    Abstract: Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the ... ...

    Abstract Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the emergence of novel variants. In addition, the spillover and subsequent adaptation of SARS-CoV-2 in livestock could significantly impact food security as well as animal and public health. Therefore, it is essential to monitor livestock species for SARS-CoV-2 spillover. We developed and optimized species-specific indirect ELISAs (iELISAs) to detect anti-SARS-CoV-2 antibodies in cattle, swine, and chickens using the spike protein receptor-binding domain (RBD) antigen. Serum samples collected prior to the COVID-19 pandemic were used to determine the cut-off threshold. RBD hyperimmunized sera from cattle (n = 3), swine (n = 6), and chicken (n = 3) were used as the positive controls. The iELISAs were evaluated compared to a live virus neutralization test using cattle (n = 150), swine (n = 150), and chicken (n = 150) serum samples collected during the COVID-19 pandemic. The iELISAs for cattle, swine, and chicken were found to have 100% sensitivity and specificity. These tools facilitate the surveillance that is necessary to quickly identify spillovers into the three most important agricultural species worldwide.
    Schlagwörter COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; antigens ; blood serum ; cattle ; chickens ; food security ; monitoring ; neutralization tests ; public health ; swine ; viruses ; wild animals
    Sprache Englisch
    Erscheinungsverlauf 2022-0622
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14071358
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel ; Online: Development and Validation of Indirect Enzyme-Linked Immunosorbent Assays for Detecting Antibodies to SARS-CoV-2 in Cattle, Swine, and Chicken.

    Gontu, Abhinay / Marlin, Erika A / Ramasamy, Santhamani / Neerukonda, Sabarinath / Anil, Gayatri / Morgan, Jasmine / Quraishi, Meysoon / Chen, Chen / Boorla, Veda Sheersh / Nissly, Ruth H / Jakka, Padmaja / Chothe, Shubhada K / Ravichandran, Abirami / Kodali, Nishitha / Amirthalingam, Saranya / LaBella, Lindsey / Kelly, Kathleen / Natesan, Pazhanivel / Minns, Allen M /
    Rossi, Randall M / Werner, Jacob R / Hovingh, Ernest / Lindner, Scott E / Tewari, Deepanker / Kapur, Vivek / Vandegrift, Kurt J / Maranas, Costas D / Surendran Nair, Meera / Kuchipudi, Suresh V

    Viruses

    2022  Band 14, Heft 7

    Abstract: Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the ... ...

    Abstract Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the emergence of novel variants. In addition, the spillover and subsequent adaptation of SARS-CoV-2 in livestock could significantly impact food security as well as animal and public health. Therefore, it is essential to monitor livestock species for SARS-CoV-2 spillover. We developed and optimized species-specific indirect ELISAs (iELISAs) to detect anti-SARS-CoV-2 antibodies in cattle, swine, and chickens using the spike protein receptor-binding domain (RBD) antigen. Serum samples collected prior to the COVID-19 pandemic were used to determine the cut-off threshold. RBD hyperimmunized sera from cattle (
    Mesh-Begriff(e) Animals ; Antibodies, Viral ; COVID-19/diagnosis ; COVID-19/veterinary ; Cattle ; Chickens ; Enzyme-Linked Immunosorbent Assay ; Humans ; Pandemics/prevention & control ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Swine
    Chemische Substanzen Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Sprache Englisch
    Erscheinungsdatum 2022-06-22
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14071358
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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