LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: CD11c

    Sosa-Hernández, Víctor A / Romero-Ramírez, Sandra / Cervantes-Díaz, Rodrigo / Carrillo-Vázquez, Daniel A / Navarro-Hernandez, Itze C / Whittall-García, Laura P / Absalón-Aguilar, Abdiel / Vargas-Castro, Ana S / Reyes-Huerta, Raúl F / Juárez-Vega, Guillermo / Meza-Sánchez, David E / Ortiz-Navarrete, Vianney / Torres-Ruiz, Jiram / Mejía-Domínguez, Nancy R / Gómez-Martín, Diana / Maravillas-Montero, José L

    Frontiers in immunology

    2022  Volume 13, Page(s) 892241

    Abstract: Lupus nephritis (LN) is one of the most common manifestations of systemic lupus erythematosus (SLE), characterized by abnormal B cell activation and differentiation to memory or plasma effector cells. However, the role of these cells in the pathogenesis ... ...

    Abstract Lupus nephritis (LN) is one of the most common manifestations of systemic lupus erythematosus (SLE), characterized by abnormal B cell activation and differentiation to memory or plasma effector cells. However, the role of these cells in the pathogenesis of LN is not fully understood, as well as the effect of induction therapy on B cell subsets, possibly associated with this manifestation, like aged-associated B cells (ABCs). Consequently, we analyzed the molecules defining the ABCs subpopulation (CD11c, T-bet, and CD21) through flow cytometry of blood samples from patients with lupus presenting or not LN, following up a small sub-cohort after six months of induction therapy. The frequency of ABCs resulted higher in LN patients compared to healthy subjects. Unexpectedly, we identified a robust reduction of a CD21
    MeSH term(s) Aged ; B-Lymphocyte Subsets ; Biomarkers ; CD11c Antigen ; Complement System Proteins/therapeutic use ; Humans ; Lupus Erythematosus, Systemic/complications ; Lupus Erythematosus, Systemic/diagnosis ; Lupus Erythematosus, Systemic/drug therapy ; Lupus Nephritis/diagnosis ; Renal Insufficiency
    Chemical Substances Biomarkers ; CD11c Antigen ; Complement System Proteins (9007-36-7)
    Language English
    Publishing date 2022-05-19
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.892241
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Tetraspanin 33 (TSPAN33) regulates endocytosis and migration of human B lymphocytes by affecting the tension of the plasma membrane

    Navarro‐Hernandez, Itze C / López‐Ortega, Orestes / Acevedo‐Ochoa, Ernesto / Cervantes‐Díaz, Rodrigo / Romero‐Ramírez, Sandra / Sosa‐Hernández, Víctor A / Meza‐Sánchez, David E / Juárez‐Vega, Guillermo / Pérez‐Martínez, César A / Chávez‐Munguía, Bibiana / Galván‐Hernández, Arturo / Antillón, Armando / Ortega‐Blake, Iván / Santos‐Argumedo, Leopoldo / Hernández‐Hernández, José M / Maravillas‐Montero, José L

    FEBS journal. 2020 Aug., v. 287, no. 16

    2020  

    Abstract: B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody‐secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, ... ...

    Abstract B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody‐secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, regulated by the formation of different lipid‐bilayer subdomains that are associated with the underlying cytoskeleton through different linker molecules, thus allowing the functional specialization of regions within the membrane. Among these, there are tetraspanin‐enriched domains. Tetraspanins constitute a superfamily of transmembrane proteins that establish lateral associations with other molecules, determining its activity and localization. In this study, we identified TSPAN33 as an active player during B‐lymphocyte cytoskeleton and plasma membrane‐related phenomena, including protrusion formation, adhesion, phagocytosis, and cell motility. By using an overexpression model of TSPAN33 in human Raji cells, we detected a specific distribution of this protein that includes membrane microvilli, the Golgi apparatus, and extracellular vesicles. Additionally, we identified diminished phagocytic ability and altered cell adhesion properties due to the aberrant expression of integrins. Accordingly, these cells presented an enhanced migratory phenotype, as shown by its augmented chemotaxis and invasion rates. When we evaluated the mechanic response of cells during fibronectin‐induced spreading, we found that TSPAN33 expression inhibited changes in roughness and membrane tension. Contrariwise, TSPAN33 knockdown cells displayed opposite phenotypes to those observed in the overexpression model. Altogether, our data indicate that TSPAN33 represents a regulatory element of the adhesion and migration of B lymphocytes, suggesting a novel implication of this tetraspanin in the control of the mechanical properties of their plasma membrane.
    Keywords B-lymphocytes ; Golgi apparatus ; adhesion ; cell adhesion ; cell movement ; chemotaxis ; cytoskeleton ; humans ; integrins ; microvilli ; migratory behavior ; models ; phagocytosis ; phenotype ; plasma membrane ; roughness
    Language English
    Dates of publication 2020-08
    Size p. 3449-3471.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note NAL-AP-2-clean ; JOURNAL ARTICLE
    ZDB-ID 2173655-8
    ISSN 1742-4658 ; 1742-464X
    ISSN (online) 1742-4658
    ISSN 1742-464X
    DOI 10.1111/febs.15216
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 2006/704: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Tetraspanin 33 (TSPAN33) regulates endocytosis and migration of human B lymphocytes by affecting the tension of the plasma membrane.

    Navarro-Hernandez, Itze C / López-Ortega, Orestes / Acevedo-Ochoa, Ernesto / Cervantes-Díaz, Rodrigo / Romero-Ramírez, Sandra / Sosa-Hernández, Víctor A / Meza-Sánchez, David E / Juárez-Vega, Guillermo / Pérez-Martínez, César A / Chávez-Munguía, Bibiana / Galván-Hernández, Arturo / Antillón, Armando / Ortega-Blake, Iván / Santos-Argumedo, Leopoldo / Hernández-Hernández, José M / Maravillas-Montero, José L

    The FEBS journal

    2020  Volume 287, Issue 16, Page(s) 3449–3471

    Abstract: B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody-secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, ... ...

    Abstract B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody-secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, regulated by the formation of different lipid-bilayer subdomains that are associated with the underlying cytoskeleton through different linker molecules, thus allowing the functional specialization of regions within the membrane. Among these, there are tetraspanin-enriched domains. Tetraspanins constitute a superfamily of transmembrane proteins that establish lateral associations with other molecules, determining its activity and localization. In this study, we identified TSPAN33 as an active player during B-lymphocyte cytoskeleton and plasma membrane-related phenomena, including protrusion formation, adhesion, phagocytosis, and cell motility. By using an overexpression model of TSPAN33 in human Raji cells, we detected a specific distribution of this protein that includes membrane microvilli, the Golgi apparatus, and extracellular vesicles. Additionally, we identified diminished phagocytic ability and altered cell adhesion properties due to the aberrant expression of integrins. Accordingly, these cells presented an enhanced migratory phenotype, as shown by its augmented chemotaxis and invasion rates. When we evaluated the mechanic response of cells during fibronectin-induced spreading, we found that TSPAN33 expression inhibited changes in roughness and membrane tension. Contrariwise, TSPAN33 knockdown cells displayed opposite phenotypes to those observed in the overexpression model. Altogether, our data indicate that TSPAN33 represents a regulatory element of the adhesion and migration of B lymphocytes, suggesting a novel implication of this tetraspanin in the control of the mechanical properties of their plasma membrane.
    MeSH term(s) B-Lymphocytes/metabolism ; B-Lymphocytes/ultrastructure ; CRISPR-Cas Systems ; Cell Adhesion/genetics ; Cell Line, Tumor ; Cell Membrane/metabolism ; Cell Movement/genetics ; Endocytosis/genetics ; Gene Knockdown Techniques ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Humans ; Microscopy, Confocal ; Microscopy, Electron ; Phagocytosis/genetics ; Stress, Mechanical ; Tetraspanins/genetics ; Tetraspanins/metabolism
    Chemical Substances TSPAN33 protein, human ; Tetraspanins ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2020-02-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2173655-8
    ISSN 1742-4658 ; 1742-464X
    ISSN (online) 1742-4658
    ISSN 1742-464X
    DOI 10.1111/febs.15216
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 260: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Innate-like B cell subsets during immune responses: Beyond antibody production.

    Romero-Ramírez, Sandra / Navarro-Hernandez, Itze C / Cervantes-Díaz, Rodrigo / Sosa-Hernández, Víctor A / Acevedo-Ochoa, Ernesto / Kleinberg-Bild, Ari / Valle-Rios, Ricardo / Meza-Sánchez, David E / Hernández-Hernández, José M / Maravillas-Montero, José L

    Journal of leukocyte biology

    2018  Volume 105, Issue 5, Page(s) 843–856

    Abstract: B lymphocytes are recognized for their crucial role in the adaptive immunity since they represent the only leukocyte lineage capable of differentiating into Ab-secreting cells. However, it has been demonstrated that these lymphocytes can exert several Ab- ...

    Abstract B lymphocytes are recognized for their crucial role in the adaptive immunity since they represent the only leukocyte lineage capable of differentiating into Ab-secreting cells. However, it has been demonstrated that these lymphocytes can exert several Ab-independent functions, including engulfing and processing Ags for presentation to T cells, secreting soluble mediators, providing co-stimulatory signals, and even participating in lymphoid tissues development. Beyond that, several reports claiming the existence of multiple B cell subsets contributing directly to innate immune responses have appeared. These "innate-like" B lymphocytes, whose phenotype, development pathways, tissue distribution, and functions are in most cases notoriously different from those of conventional B cells, are crucial to early protective responses against pathogens by exerting "crossover" defensive strategies that blur the established boundaries of innate and adaptive branches of immunity. Examples of these mechanisms include the rapid secretion of the polyspecific natural Abs, increased susceptibility to innate receptors-mediated activation, cytokine secretion, downstream priming of other innate cells, usage of specific variable immunoglobulin gene-segments, and other features. As these new insights emerge, it is becoming preponderant to redefine the functionality of B cells beyond their classical adaptive-immune tasks.
    MeSH term(s) Animals ; Antibodies/genetics ; Antibodies/immunology ; Antigens, CD/genetics ; Antigens, CD/immunology ; B-Lymphocyte Subsets/classification ; B-Lymphocyte Subsets/cytology ; B-Lymphocyte Subsets/immunology ; Cell Communication/immunology ; Cell Lineage/genetics ; Cell Lineage/immunology ; Cytokines/genetics ; Cytokines/immunology ; Gene Expression ; Humans ; Immunity, Cellular ; Immunity, Humoral ; Immunity, Innate
    Chemical Substances Antibodies ; Antigens, CD ; Cytokines
    Language English
    Publishing date 2018-11-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 605722-6
    ISSN 1938-3673 ; 0741-5400
    ISSN (online) 1938-3673
    ISSN 0741-5400
    DOI 10.1002/JLB.MR0618-227R
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 603: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top