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  1. Article: Rice husk-derived mesoporous biogenic silica nanoparticles for gravity chromatography.

    Shrestha, Devendra / Nayaju, Tulsi / Kandel, M R / Pradhananga, Raja Ram / Park, Chan Hee / Kim, Cheol Sang

    Heliyon

    2023  Volume 9, Issue 4, Page(s) e15142

    Abstract: Biogenic silica nanoparticle is a superb alternative to synthetic silica because of their highly active, polar, and porous nanostructure with a large interior area. Among the available agricultural bioresources, biogenic silica extracted from rice husks ... ...

    Abstract Biogenic silica nanoparticle is a superb alternative to synthetic silica because of their highly active, polar, and porous nanostructure with a large interior area. Among the available agricultural bioresources, biogenic silica extracted from rice husks could be a simple, easily available, and cost-effective resource to use as the stationary phase for the column chromatographic technique. In the present study, highly pure amorphous biogenic silica nanoparticles (bSNPs) were synthesized using rice husk by a controlled combustion route followed by the sol-gel method. The bSNPs show better performance for the separation and isolation of ortho- and
    Language English
    Publishing date 2023-04-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e15142
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Rice husk-derived mesoporous biogenic silica nanoparticles for gravity chromatography

    Shrestha, Devendra / Nayaju, Tulsi / Kandel, M.R. / Pradhananga, Raja Ram / Park, Chan-Hee / Kim, Cheol Sang

    Heliyon. 20232023 Apr. 01, Apr. 01, v. 9, no. 4 p.e15142-

    2023  

    Abstract: Biogenic silica nanoparticle is a superb alternative to synthetic silica because of their highly active, polar, and porous nanostructure with a large interior area. Among the available agricultural bioresources, biogenic silica extracted from rice husks ... ...

    Abstract Biogenic silica nanoparticle is a superb alternative to synthetic silica because of their highly active, polar, and porous nanostructure with a large interior area. Among the available agricultural bioresources, biogenic silica extracted from rice husks could be a simple, easily available, and cost-effective resource to use as the stationary phase for the column chromatographic technique. In the present study, highly pure amorphous biogenic silica nanoparticles (bSNPs) were synthesized using rice husk by a controlled combustion route followed by the sol-gel method. The bSNPs show better performance for the separation and isolation of ortho- and para-nitrophenol and nitroaniline. The outstanding performance of the as-synthesized bSNPs is attributed to the high surface area, high porosity, and presence of Si-OH polar bonds. These preliminary findings imply that rice husk, an agricultural waste, could be an alternative source of silica and applicable as a stationary phase in column chromatography.
    Keywords agricultural wastes ; chromatography ; combustion ; cost effectiveness ; gravity ; nanoparticles ; nitroanilines ; porosity ; porous media ; rice ; rice hulls ; silica ; sol-gel processing ; surface area ; Biogenic silica nanoparticles ; Rice husk silica ; Column chromatography ; Sol-gel ; Amorphous
    Language English
    Dates of publication 2023-0401
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e15142
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Higher Prevalence of Extended Spectrum β-Lactamase Producing Uropathogenic Escherichia coli Among Patients with Diabetes from a Tertiary Care Hospital of Kathmandu, Nepal.

    Nayaju, Tulsi / Upreti, Milan Kumar / Ghimire, Alina / Shrestha, Basudha / Maharjan, Basanta / Joshi, Rajesh Dhoj / Lekhak, Binod / Thapa Shrestha, Upendra

    The American journal of tropical medicine and hygiene

    2021  Volume 105, Issue 5, Page(s) 1347–1355

    Abstract: This study aimed to determine the occurrence of antibiotic resistance genes for β-lactamases; blaTEM and blaCTX-M in uropathogenic Escherichia coli isolates from urinary tract infection (UTI) suspected diabetic and nondiabetic patients. A hospital-based ... ...

    Abstract This study aimed to determine the occurrence of antibiotic resistance genes for β-lactamases; blaTEM and blaCTX-M in uropathogenic Escherichia coli isolates from urinary tract infection (UTI) suspected diabetic and nondiabetic patients. A hospital-based cross-sectional study was conducted in Kathmandu Model Hospital, Kathmandu, in association with the Department of Microbiology, GoldenGate International College, Kathmandu, Nepal, from June to December 2018. A total of 1,267 nonduplicate midstream urine specimens were obtained and processed immediately for isolation of uropathogens. The isolates were subjected to antibiotic susceptibility testing and extended spectrum β-lactamase (ESBL) confirmation. In addition, blaTEM and blaCTX-M genes were detected using specific primers. The overall prevalence of UTI was 17.2% (218/1,267), of which patients with diabetes were significantly more infected; 32.3% (31/96) as compared with nonpatients with diabetes, 15.9% (187/1,171). A total of 221 bacterial isolates were obtained from 218 culture-positive specimens in which E. coli was the most predominant; 67.9% (150/221). Forty-four percent (66/150) of the total E. coli was multidrug resistant and 37.3% (56/150) were ESBL producers. Among 56 isolates, 92.3% (12/13) were from patients with diabetes, and 83.0% (44/53) were from nondiabetics. Furthermore, 84.9% of the screened ESBL producers were confirmed to possess either single or both of blaTEM and blaCTX-M genes. The blaTEM and blaCTX-M genes were detected in 53.6% and 87.5% of the phenotypically ESBL confirmed E. coli, respectively. Higher rates of ESBL producing uropathogenic E. coli are associated among patients with diabetes causing an alarming situation for disease management. However, second-line drugs with broad antimicrobial properties are still found to be effective drugs for multidrug resistance strains.
    MeSH term(s) Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Anti-Infective Agents/therapeutic use ; Child ; Child, Preschool ; Cross-Sectional Studies ; Diabetes Complications/drug therapy ; Diabetes Complications/epidemiology ; Drug Resistance, Multiple, Bacterial/drug effects ; Drug Resistance, Multiple, Bacterial/genetics ; Escherichia coli Infections/drug therapy ; Escherichia coli Infections/epidemiology ; Female ; Healthy Volunteers ; Humans ; Infant ; Male ; Middle Aged ; Nepal/epidemiology ; Prevalence ; Sex Factors ; Tertiary Care Centers ; Urinary Tract Infections/drug therapy ; Urinary Tract Infections/epidemiology ; Uropathogenic Escherichia coli/drug effects ; Uropathogenic Escherichia coli/genetics ; Young Adult ; beta-Lactamases/genetics ; beta-Lactamases/metabolism
    Chemical Substances Anti-Infective Agents ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2021-08-23
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2942-7
    ISSN 1476-1645 ; 0002-9637
    ISSN (online) 1476-1645
    ISSN 0002-9637
    DOI 10.4269/ajtmh.21-0691
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Microbial and Parasitic Contamination of Fresh Raw Vegetable Samples and Detection of the BlaTEM and BlaCTX-M Genes from E. coli Isolates

    Ghimire, Alina / Upadhyaya, Jitendra / Nayaju, Tulsi / Lekhak, Binod / Chaudhary, Dhiraj Kumar / Raghavan, Vijaya / Pant, Bhoj Raj / Bajgai, Tirtha Raj / Koirala, Niranjan / Upreti, Milan Kumar

    Agriculture (Basel). 2020 Aug. 07, v. 10, no. 8

    2020  

    Abstract: A total of 100 fresh-raw vegetable samples were collected from the Kathmandu, Lalitpur and Bhaktapur districts of Nepal to evaluate microbial and parasitic contamination, presence of Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli and ... ...

    Abstract A total of 100 fresh-raw vegetable samples were collected from the Kathmandu, Lalitpur and Bhaktapur districts of Nepal to evaluate microbial and parasitic contamination, presence of Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli and detect the blaTEM and blaCTX₋M genes among the Escherichia coli isolates. This study revealed that the prevalence of Giardia cysts was highest (100%) and Hookworm and Entamoeba coli were lowest (1% each). Coliforms were isolated from every raw vegetable sample. A total of 178 bacterial isolates were isolated among which 57 isolates were identified as E. coli, out of which 33 were Multi-drug resistant (MDR) isolates. The high rate of resistance was found towards amoxicillin/clavulanate, tetracycline and cotrimoxazole. The 10 E. coli isolates tested positive in an ESBL screening, out of which 4 were confirmed as ESBL producers by a combined disc test. Out of these 4 confirmed ESBL E. coli, one was found to carry both the blaTEM gene and blaCTX₋M genes by the Polymerase Chain Reaction (PCR) technique. One isolate has only the blaTEM gene, while other isolate harboured only blaCTX₋M genes.
    Keywords Entamoeba ; Escherichia coli ; Giardia ; agriculture ; amoxicillin ; genes ; hookworms ; multiple drug resistance ; polymerase chain reaction ; tetracycline ; vegetables ; Nepal
    Language English
    Dates of publication 2020-0807
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2651678-0
    ISSN 2077-0472
    ISSN 2077-0472
    DOI 10.3390/agriculture10080341
    Database NAL-Catalogue (AGRICOLA)

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