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  1. Book ; Online: Compositional Servoing by Recombining Demonstrations

    Argus, Max / Nayak, Abhijeet / Büchner, Martin / Galesso, Silvio / Valada, Abhinav / Brox, Thomas

    2023  

    Abstract: Learning-based manipulation policies from image inputs often show weak task transfer capabilities. In contrast, visual servoing methods allow efficient task transfer in high-precision scenarios while requiring only a few demonstrations. In this work, we ... ...

    Abstract Learning-based manipulation policies from image inputs often show weak task transfer capabilities. In contrast, visual servoing methods allow efficient task transfer in high-precision scenarios while requiring only a few demonstrations. In this work, we present a framework that formulates the visual servoing task as graph traversal. Our method not only extends the robustness of visual servoing, but also enables multitask capability based on a few task-specific demonstrations. We construct demonstration graphs by splitting existing demonstrations and recombining them. In order to traverse the demonstration graph in the inference case, we utilize a similarity function that helps select the best demonstration for a specific task. This enables us to compute the shortest path through the graph. Ultimately, we show that recombining demonstrations leads to higher task-respective success. We present extensive simulation and real-world experimental results that demonstrate the efficacy of our approach.

    Comment: http://compservo.cs.uni-freiburg.de
    Keywords Computer Science - Robotics ; Computer Science - Computer Vision and Pattern Recognition
    Subject code 004
    Publishing date 2023-10-06
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Broadly Protective Multivalent OspA Vaccine against Lyme Borreliosis, Developed Based on Surface Shaping of the C-Terminal Fragment.

    Nayak, Abhijeet / Schüler, Wolfgang / Seidel, Stefan / Gomez, Ivan / Meinke, Andreas / Comstedt, Pär / Lundberg, Urban

    Infection and immunity

    2020  Volume 88, Issue 4

    Abstract: The development of vaccines for prevention of diseases caused by pathogenic species can encounter major obstacles if high sequence diversity is observed between individual strains. Therefore, development might be restricted either to conserved antigens, ... ...

    Abstract The development of vaccines for prevention of diseases caused by pathogenic species can encounter major obstacles if high sequence diversity is observed between individual strains. Therefore, development might be restricted either to conserved antigens, which are often rare, or to multivalent vaccines, which renders the production more costly and cumbersome. In light of this complexity, we applied a structure-based surface shaping approach for the development of a Lyme borreliosis (LB) vaccine suitable for the United States and Europe. The surface of the C-terminal fragment of outer surface protein A (OspA) was divided into distinct regions, based primarily on binding sites of monoclonal antibodies (MAbs). In order to target the six clinically most relevant OspA serotypes (ST) in a single protein, exposed amino acids of the individual regions were exchanged to corresponding amino acids of a chosen OspA serotype. Six chimeric proteins were constructed, and, based on their immunogenicity, four of these chimeras were tested in mouse challenge models. Significant protection could be demonstrated for all four proteins following challenge with infected ticks (OspA ST1, OspA ST2, and OspA ST4) or with
    MeSH term(s) Animals ; Antigens, Surface/administration & dosage ; Antigens, Surface/genetics ; Antigens, Surface/immunology ; Bacterial Outer Membrane Proteins/administration & dosage ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/immunology ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/genetics ; Bacterial Vaccines/immunology ; Borrelia/genetics ; Borrelia/immunology ; Disease Models, Animal ; Lipoproteins/administration & dosage ; Lipoproteins/genetics ; Lipoproteins/immunology ; Lyme Disease/prevention & control ; Mice ; Protein Engineering ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Vaccines, Synthetic/administration & dosage ; Vaccines, Synthetic/genetics ; Vaccines, Synthetic/immunology
    Chemical Substances Antigens, Surface ; Bacterial Outer Membrane Proteins ; Bacterial Vaccines ; Lipoproteins ; OspA protein ; Recombinant Proteins ; Vaccines, Synthetic
    Language English
    Publishing date 2020-03-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.00917-19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Ixodes ricinus salivary gland proteome during feeding and B. Afzelii infection: New avenues for an anti-tick vaccine.

    Klouwens, Michelle J / Trentelman, Jos J A / Barriales, Diego / Ersoz, Jasmin I / Azkargorta, Mikel / Elortza, Felix / Šíma, Radek / Hajdušek, Ondrej / Lavin, José-Luis / Tomás Cortazar, Julen / Escobes Corcuera, Iraide / Colstrup, Emil / Nayak, Abhijeet / Martín Ruíz, Itziar / Rodriguez, Hector / Nijhof, Ard M / Anguita, Juan / Hovius, Joppe W R

    Vaccine

    2023  Volume 41, Issue 12, Page(s) 1951–1960

    Abstract: Introduction: Borrelia burgdorferi sensu lato, the causative agents of Lyme borreliosis, are transmitted by Ixodes ticks. Tick saliva proteins are instrumental for survival of both the vector and spirochete and have been investigated as targets for ... ...

    Abstract Introduction: Borrelia burgdorferi sensu lato, the causative agents of Lyme borreliosis, are transmitted by Ixodes ticks. Tick saliva proteins are instrumental for survival of both the vector and spirochete and have been investigated as targets for vaccine targeting the vector. In Europe, the main vector for Lyme borreliosis is Ixodes ricinus, which predominantly transmits Borrelia afzelii. We here investigated the differential production of I. ricinus tick saliva proteins in response to feeding and B. afzelii infection.
    Method: Label-free Quantitative Proteomics and Progenesis QI software was used to identify, compare, and select tick salivary gland proteins differentially produced during tick feeding and in response to B. afzelii infection. Tick saliva proteins were selected for validation, recombinantly expressed and used in both mouse and guinea pig vaccination and tick-challenge studies.
    Results: We identified 870 I. ricinus proteins from which 68 were overrepresented upon 24-hours of feeding and B. afzelii infection. Selected tick proteins were successfully validated by confirming their expression at the RNA and native protein level in independent tick pools. When used in a recombinant vaccine formulation, these tick proteins significantly reduced the post-engorgement weights of I. ricinus nymphs in two experimental animal models. Despite the reduced ability of ticks to feed on vaccinated animals, we observed efficient transmission of B. afzelii to the murine host.
    Conclusion: Using quantitative proteomics, we identified differential protein production in I. ricinus salivary glands in response to B. afzelii infection and different feeding conditions. These results provide novel insights into the process of I. ricinus feeding and B. afzelii transmission and revealed novel candidates for an anti-tick vaccine.
    MeSH term(s) Animals ; Guinea Pigs ; Mice ; Ixodes ; Proteome ; Arachnid Vectors ; Lyme Disease/prevention & control ; Salivary Glands ; Arthropod Proteins ; Vaccines
    Chemical Substances Proteome ; Arthropod Proteins ; Vaccines
    Language English
    Publishing date 2023-02-14
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2023.02.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Identification of novel conserved Ixodes vaccine candidates; a promising role for non-secreted salivary gland proteins.

    Trentelman, Jos J A / de Vogel, Fons A / Colstrup, Emil / Sima, Radek / Coumou, Jeroen / Koetsveld, Joris / Klouwens, Michelle J / Nayak, Abhijeet / Ersoz, Jasmin / Barriales, Diego / Tomás-Cortázar, Julen / Narasimhan, Sukanya / Hajdusek, Ondrej / Anguita, Juan / Hovius, Joppe W

    Vaccine

    2022  Volume 40, Issue 52, Page(s) 7593–7603

    Abstract: Ixodes ricinus and Ixodes scapularis are the main vectors for the causative agents of Lyme borreliosis and a wide range of other pathogens. Repeated tick-bites are known to lead to tick rejection; a phenomenon designated as tick immunity. Tick immunity ... ...

    Abstract Ixodes ricinus and Ixodes scapularis are the main vectors for the causative agents of Lyme borreliosis and a wide range of other pathogens. Repeated tick-bites are known to lead to tick rejection; a phenomenon designated as tick immunity. Tick immunity is mainly directed against tick salivary gland proteins (TSGPs) and has been shown to partially protect against experimental Lyme borreliosis. TSGPs recognized by antibodies from tick immune animals could therefore be interesting candidates for an anti-tick vaccine, which might also block pathogen transmission. To identify conserved Ixodes TSGPs that could serve as a universal anti-tick vaccine in both Europe and the US, a Yeast Surface Display containing salivary gland genes of nymphal I. ricinus expressed at 24, 48 and 72 h into tick feeding was probed with either sera from rabbits repeatedly exposed for 24 h to I. ricinus nymphal ticks and/or sera from rabbits immune to I. scapularis. Thus, we identified thirteen TSGP vaccine candidates, of which ten were secreted. For vaccination studies in rabbits, we selected six secreted TSGPs, five full length and one conserved peptide. None of these proteins hampered tick feeding. In contrast, vaccination of guinea pigs with four non-secreted TSGPs - two from the current and two from a previous human immunoscreening - did significantly reduce tick attachment and feeding. Therefore, non-secreted TSGPs appear to be involved in the development of tick immunity and are interesting candidates for an anti-tick vaccine.
    MeSH term(s) Animals ; Guinea Pigs ; Humans ; Rabbits ; Ixodes ; Lyme Disease/prevention & control ; Salivary Glands ; Salivary Proteins and Peptides/genetics ; Salivary Proteins and Peptides/metabolism ; Vaccines
    Chemical Substances Salivary Proteins and Peptides ; Vaccines
    Language English
    Publishing date 2022-11-08
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2022.10.032
    Database MEDical Literature Analysis and Retrieval System OnLINE

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