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Article ; Online: The Zebrafish as a Model for Human Bacterial Infections.

Neely, Melody N

Methods in molecular biology (Clifton, N.J.)

2017 Volume 1535, Page(s) 245–266

Abstract: The development of the zebrafish (Danio rerio) infectious disease model has provided new insights and information into pathogenesis. Many of these new discoveries would not have been possible using a typical mammalian model. The advantages of using this ... ...

Abstract	The development of the zebrafish (Danio rerio) infectious disease model has provided new insights and information into pathogenesis. Many of these new discoveries would not have been possible using a typical mammalian model. The advantages of using this model are many and in the last 15 years the model has been exploited for the analysis of many different pathogens. Here, we describe in detail how to perform a bacterial infection using either the adult zebrafish or zebrafish larvae using microinjection. Multiple methods of analysis are described that can be used to address specific questions pertaining to disease progression and the interactions with the immune system.
Language	English
Publishing date	2017
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-6673-8_16
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The LCP Family Protein, Psr, Is Required for Cell Wall Integrity and Virulence in

Rajaei, Atefeh / Rowe, Hannah M / Neely, Melody N

Microorganisms

2022 Volume 10, Issue 2

Abstract: A robust cell envelope is the first line of protection for an infecting pathogen when encountering the immune defense of its host. In Gram-positive organisms, LytR-CpsA-Psr (LCP) family proteins play a major role in the synthesis and assembly of the cell ...

Abstract	A robust cell envelope is the first line of protection for an infecting pathogen when encountering the immune defense of its host. In Gram-positive organisms, LytR-CpsA-Psr (LCP) family proteins play a major role in the synthesis and assembly of the cell envelope. While these proteins could be considered for potential new drug targets, not enough is known about how they function to support the integrity of the cell wall.
Language	English
Publishing date	2022-01-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10020217
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Article: The LCP Family Protein, Psr, Is Required for Cell Wall Integrity and Virulence in Streptococcus agalactiae

Rajaei, Atefeh / Rowe, Hannah M. / Neely, Melody N.

Microorganisms. 2022 Jan. 20, v. 10, no. 2

2022

Abstract	A robust cell envelope is the first line of protection for an infecting pathogen when encountering the immune defense of its host. In Gram-positive organisms, LytR-CpsA-Psr (LCP) family proteins play a major role in the synthesis and assembly of the cell envelope. While these proteins could be considered for potential new drug targets, not enough is known about how they function to support the integrity of the cell wall. Streptococcus agalactiae (group B streptococcus or GBS) is known to encode at least three LCP family proteins, including CpsA, LytR (BrpA) and Psr. Using strains of GBS that have mutations in two of the three LCP proteins, we were able to determine a role for these proteins in GBS cell wall integrity. The results presented here demonstrate that the absence of Psr results in a decreased growth rate, decreased viability over time, inconsistent cocci morphology and diminished cell wall integrity, as well as an increased penicillin susceptibility, decreased capsule levels and attenuation in virulence in a zebrafish model of infectious disease. A strain that is missing two of the LCP family proteins, CpsA and Psr, exhibits an increase in these defective phenotypes, indicating that CpsA and Psr are partially redundant in function.
Keywords	Danio rerio ; Streptococcus agalactiae ; cell walls ; immune response ; infectious diseases ; models ; pathogens ; penicillins ; viability ; virulence
Language	English
Dates of publication	2022-0120
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10020217
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Genome Sequence of Singleton Gordonia terrae Bacteriophage Finkle.

Ashby, Seth / Bressette, Gavin / Brown, Sydney / Charles, Sophie / Maginnis, Melissa / Molloy, Sally D / Neely, Melody N

Microbiology resource announcements

2022 Volume 11, Issue 9, Page(s) e0069322

Abstract: Bacteriophage Finkle is a temperate siphovirus isolated from soil on Gordonia terrae. The 47,895-bp genome has a GC content of 66.6% and encodes 84 protein-coding genes. The genome is not closely related to sequences in the Actinobacteriophage database, ... ...

Abstract	Bacteriophage Finkle is a temperate siphovirus isolated from soil on Gordonia terrae. The 47,895-bp genome has a GC content of 66.6% and encodes 84 protein-coding genes. The genome is not closely related to sequences in the Actinobacteriophage database, sharing less than 35% gene content, and was classified as a singleton.
Language	English
Publishing date	2022-08-25
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/mra.00693-22
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Article ; Online: Genome Sequence of the Cluster CD

Curtis, Ben / Ganbaatar, Bayarjavkhlan / Lawrence, Griffin / Oglesby, Wyatt / Rice, Jaylee / Tremblay, Emelia / Maginnis, Melissa / Molloy, Sally D / Neely, Melody N

Microbiology resource announcements

2022 Volume 11, Issue 10, Page(s) e0069522

Abstract: Widow is a novel cluster CD bacteriophage isolated from a soil sample using the bacterial host Gordonia terrae. The Widow genome is 43,656 bp in length and encodes 64 protein-coding genes and no tRNAs. The genome shares 52 to 92% gene content with other ... ...

Abstract	Widow is a novel cluster CD bacteriophage isolated from a soil sample using the bacterial host Gordonia terrae. The Widow genome is 43,656 bp in length and encodes 64 protein-coding genes and no tRNAs. The genome shares 52 to 92% gene content with other cluster CD members.
Language	English
Publishing date	2022-09-06
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/mra.00695-22
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Article ; Online: Characterization of the genomes of cluster CT

Bazinet, Nicole / Biro, Esther / Geydoshek, Ashley / Hodgkin, Grace / Jestel, Eric / Klute, Emily / MacDonald, Clare / Russano, Marcus / Thatcher, Jayson / Neely, Melody N / Molloy, Sally

Microbiology resource announcements

2023 Volume 12, Issue 12, Page(s) e0074723

Abstract: The genomes of lytic, cluster ... ...

Abstract	The genomes of lytic, cluster CT
Language	English
Publishing date	2023-11-20
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/MRA.00747-23
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Article ; Online: Coordinate regulation of Gram-positive cell surface components.

Hanson, Brett R / Neely, Melody N

Current opinion in microbiology

2012 Volume 15, Issue 2, Page(s) 204–210

Abstract: The cell surface of Gram-positive pathogens represents a complex association of glycopolymers that control cell division, homeostasis, immune evasion, tissue invasion, and resistance to antimicrobials. These glycopolymers include the peptidoglycan cell ... ...

Abstract	The cell surface of Gram-positive pathogens represents a complex association of glycopolymers that control cell division, homeostasis, immune evasion, tissue invasion, and resistance to antimicrobials. These glycopolymers include the peptidoglycan cell wall, wall-teichoic acids, lipoteichoic acids, and capsular polysaccharide. Disruption of individual factors often results in pleiotropic effects, making it difficult to discern regulation and function. In this review we collate recent work describing these pleiotropic phenotypes, and propose that this is due to coordinated regulation of biosynthesis or modification of these cell surface components. A better understanding of the regulatory networks that control the relative prevalence of each factor on the cell surface or their modulated functions may help facilitate the identification of new targets for antimicrobial therapy.
MeSH term(s)	Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cell Membrane/chemistry ; Cell Membrane/metabolism ; Cell Wall/chemistry ; Cell Wall/metabolism ; Gene Expression Regulation, Bacterial ; Gram-Positive Bacteria/chemistry ; Gram-Positive Bacteria/metabolism ; Humans ; Lipopolysaccharides/metabolism ; Peptidoglycan/metabolism ; Polysaccharides, Bacterial/metabolism ; Teichoic Acids/metabolism
Chemical Substances	Bacterial Proteins ; Lipopolysaccharides ; Peptidoglycan ; Polysaccharides, Bacterial ; Teichoic Acids ; lipoteichoic acid (56411-57-5)
Language	English
Publishing date	2012-01-10
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1418474-6
ISSN	1879-0364 ; 1369-5274
ISSN (online)	1879-0364
ISSN	1369-5274
DOI	10.1016/j.mib.2011.12.011
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Zs.A 5514: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 51: Show issues

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Article ; Online: CpsY influences Streptococcus iniae cell wall adaptations important for neutrophil intracellular survival.

Allen, Jonathan P / Neely, Melody N

Infection and immunity

2012 Volume 80, Issue 5, Page(s) 1707–1715

Abstract: The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development ... ...

Abstract	The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is a conserved transcriptional regulator previously identified as an important virulence determinant for systemic infection of Streptococcus iniae. While orthologs of CpsY have been associated with the regulation of methionine metabolism and uptake pathways, CpsY additionally functions in protection from neutrophil-mediated killing. S. iniae does not alter neutrophil phagosomal maturation but instead is able to adapt to the extreme bactericidal environment of a mature neutrophil phagosome, a property dependent upon CpsY. This CpsY-dependent adaptation appears to involve stabilization of the cell wall through peptidoglycan O-acetylation and repression of cellular autolysins. Furthermore, S. iniae continues to be a powerful model for investigation of bacterial adaptations during systemic streptococcal infection.
MeSH term(s)	Adaptation, Physiological/physiology ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cell Wall/metabolism ; Drug Resistance, Bacterial ; Gene Expression Regulation, Bacterial/physiology ; Mutation ; Neutrophils/microbiology ; Phagosomes ; Promoter Regions, Genetic ; Streptococcus/drug effects ; Streptococcus/physiology ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Transcription Factors
Language	English
Publishing date	2012-02-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	218698-6
ISSN	1098-5522 ; 0019-9567
ISSN (online)	1098-5522
ISSN	0019-9567
DOI	10.1128/IAI.00027-12
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 684: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.

Spencer, Brady L / Job, Alyx M / Robertson, Clare M / Hameed, Zainab A / Serchejian, Camille / Wiafe-Kwakye, Caitlin S / Mendonça, Jéssica C / Apolonio, Morgan A / Nagao, Prescilla E / Neely, Melody N / Korotkova, Natalia / Korotkov, Konstantin V / Patras, Kathryn A / Doran, Kelly S

Molecular microbiology

2023 Volume 120, Issue 2, Page(s) 258–275

Abstract: Type VIIb secretion systems (T7SSb) in Gram-positive bacteria facilitate physiology, interbacterial competition, and/or virulence via EssC ATPase-driven secretion of small ɑ-helical proteins and toxins. Recently, we characterized T7SSb in group B ... ...

Abstract	Type VIIb secretion systems (T7SSb) in Gram-positive bacteria facilitate physiology, interbacterial competition, and/or virulence via EssC ATPase-driven secretion of small ɑ-helical proteins and toxins. Recently, we characterized T7SSb in group B Streptococcus (GBS), a leading cause of infection in newborns and immunocompromised adults. GBS T7SS comprises four subtypes based on variation in the C-terminus of EssC and the repertoire of downstream effectors; however, the intraspecies diversity of GBS T7SS and impact on GBS-host interactions remains unknown. Bioinformatic analysis indicates that GBS T7SS loci encode subtype-specific putative effectors, which have low interspecies and inter-subtype homology but contain similar domains/motifs and therefore may serve similar functions. We further identify orphaned GBS WXG100 proteins. Functionally, we show that GBS T7SS subtype I and III strains secrete EsxA in vitro and that in subtype I strain CJB111, esxA1 appears to be differentially transcribed from the T7SS operon. Furthermore, we observe subtype-specific effects of GBS T7SS on host colonization, as CJB111 subtype I but not CNCTC 10/84 subtype III T7SS promotes GBS vaginal colonization. Finally, we observe that T7SS subtypes I and II are the predominant subtypes in clinical GBS isolates. This study highlights the potential impact of T7SS heterogeneity on host-GBS interactions.
MeSH term(s)	Infant, Newborn ; Female ; Humans ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Type VII Secretion Systems/genetics ; Virulence ; Operon/genetics ; Genitalia, Female/metabolism ; Streptococcal Infections/microbiology ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/metabolism ; Vagina/metabolism ; Vagina/microbiology
Chemical Substances	Bacterial Proteins ; Type VII Secretion Systems
Language	English
Publishing date	2023-06-26
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	619315-8
ISSN	1365-2958 ; 0950-382X
ISSN (online)	1365-2958
ISSN	0950-382X
DOI	10.1111/mmi.15115
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Zs.A 2502: Show issues

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Article: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.

bioRxiv : the preprint server for biology

2023

Abstract	Type VIIb secretion systems (T7SSb) in Gram-positive bacteria facilitate physiology, interbacterial competition, and/or virulence via EssC ATPase-driven secretion of small ɑ-helical proteins and toxins. Recently, we characterized T7SSb in group B
Language	English
Publishing date	2023-01-25
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.01.25.525443
Database	MEDical Literature Analysis and Retrieval System OnLINE

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