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  1. Article ; Online: Milk-Derived Extracellular Vesicles: Biomedical Applications, Current Challenges, and Future Perspectives.

    Salehi, Mahsa / Negahdari, Babak / Mehryab, Fatemeh / Shekari, Faezeh

    Journal of agricultural and food chemistry

    2024  Volume 72, Issue 15, Page(s) 8304–8331

    Abstract: Extracellular vesicles (EVs) are nano to-micrometer-sized sacs that are released by almost all animal and plant cells and act as intercellular communicators by transferring their cargos between the source and target cells. As a safe and scalable ... ...

    Abstract Extracellular vesicles (EVs) are nano to-micrometer-sized sacs that are released by almost all animal and plant cells and act as intercellular communicators by transferring their cargos between the source and target cells. As a safe and scalable alternative to conditioned medium-derived EVs, milk-derived EVs (miEVs) have recently gained a great deal of popularity. Numerous studies have shown that miEVs have intrinsic therapeutic actions that can treat diseases and enhance human health. Additionally, they can be used as natural drug carriers and novel classes of biomarkers. However, due to the complexity of the milk, the successful translation of miEVs from benchtop to bedside still faces several unfilled gaps, especially a lack of standardized protocols for the isolation of high-purity miEVs. In this work, by comprehensively reviewing the bovine miEVs studies, we provide an overview of current knowledge and research on miEVs while highlighting their challenges and enormous promise as a novel class of theranostics. It is hoped that this study will pave the way for clinical applications of miEVs by addressing their challenges and opportunities.
    MeSH term(s) Animals ; Cattle ; Humans ; Milk ; Extracellular Vesicles ; Drug Carriers ; Drug Delivery Systems/methods ; Biomarkers
    Chemical Substances Drug Carriers ; Biomarkers
    Language English
    Publishing date 2024-04-08
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.3c07899
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    Zs.A 1172: Show issues Location:
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  2. Article ; Online: Clinical and diagnostic potential of regulatory T cell markers: From bench to bedside.

    Haddadi, Mohammad Hossein / Negahdari, Babak

    Transplant immunology

    2021  Volume 70, Page(s) 101518

    Abstract: Regulatory T (Treg) cells are heterogeneous immune cell populations residing in the thymus and peripheral lymphatic tissues. This immune cell plays a central and critical role in maintaining immune tolerance against undesirable immune responses. Treg ... ...

    Abstract Regulatory T (Treg) cells are heterogeneous immune cell populations residing in the thymus and peripheral lymphatic tissues. This immune cell plays a central and critical role in maintaining immune tolerance against undesirable immune responses. Treg cells' phenotypic heterogeneity caused by different pathological conditions makes their identification and differentiation from non-suppressive T cells difficult. On the other hand, using nonspecific markers and variable isolation panels leads to undesirable outcomes. There are a variety of markers to identify functional Treg cells, including CD25, FOXP3, and CTLA-4, as well as the epigenetic signature of forkhead box P3 (FOXP3), which can be used for both natural and induced Treg cells. Phenotypic heterogeneity is a major concern in Treg purification when using nonspecific markers, which can be addressed by utilizing suitable isolation panels designed for different purposes. This review presents a clinical framework for Treg detection and isolation, focusing on Treg markers such as CD25, FOXP3, CTLA-4, CD127, GPA-33, and TSDR demethylation to design Treg isolation panels suitable for different Treg therapy purposes. The current review also highlights new reliable Treg markers applicable for different purposes.
    MeSH term(s) Biomarkers ; CD3 Complex ; Forkhead Transcription Factors ; Interleukin-2 Receptor alpha Subunit ; T-Lymphocytes, Regulatory
    Chemical Substances Biomarkers ; CD3 Complex ; Forkhead Transcription Factors ; Interleukin-2 Receptor alpha Subunit
    Language English
    Publishing date 2021-12-16
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 1160846-8
    ISSN 1878-5492 ; 0966-3274
    ISSN (online) 1878-5492
    ISSN 0966-3274
    DOI 10.1016/j.trim.2021.101518
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    Zs.A 3784: Show issues Location:
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  3. Article ; Online: Antibacterial and Immunoregulatory Effects of Metformin against

    Valadbeigi, Hassan / Khoshnood, Saeed / Negahdari, Babak / Abdullah, Mohd Azmuddin / Haddadi, Mohammad Hossein

    BioMed research international

    2023  Volume 2023, Page(s) 5583286

    Abstract: Introduction: Helicobacter pylori: Methods: Forty-five male Sprague-Dawley rats were divided into seven groups and infected with : Results: Metformin showed weak antibacterial activity against clinically isolated : Conclusions: The combination ... ...

    Abstract Introduction: Helicobacter pylori
    Methods: Forty-five male Sprague-Dawley rats were divided into seven groups and infected with
    Results: Metformin showed weak antibacterial activity against clinically isolated
    Conclusions: The combination of metformin with potential antibiotics such as AMX had a positive effect on the relief of
    MeSH term(s) Male ; Rats ; Animals ; Helicobacter Infections/drug therapy ; Helicobacter pylori ; Interleukin-8 ; Rats, Sprague-Dawley ; Anti-Bacterial Agents/pharmacology ; Amoxicillin/pharmacology ; Inflammation ; Transforming Growth Factor beta
    Chemical Substances Interleukin-8 ; Anti-Bacterial Agents ; Amoxicillin (804826J2HU) ; Transforming Growth Factor beta
    Language English
    Publishing date 2023-11-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2023/5583286
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  4. Article ; Online: Nanovaccines for cancer immunotherapy: Focusing on complex formation between adjuvant and antigen.

    Hashemi Goradel, Nasser / Nemati, Mahnaz / Bakhshandeh, Azam / Arashkia, Arash / Negahdari, Babak

    International immunopharmacology

    2023  Volume 117, Page(s) 109887

    Abstract: As an interesting cancer immunotherapy approach, cancer vaccines have been developed to deliver tumor antigens and adjuvants to antigen-presenting cells (APCs). Although the safety and easy production shifted the vaccine designing platforms toward the ... ...

    Abstract As an interesting cancer immunotherapy approach, cancer vaccines have been developed to deliver tumor antigens and adjuvants to antigen-presenting cells (APCs). Although the safety and easy production shifted the vaccine designing platforms toward the subunit vaccines, their efficacy is limited due to inefficient vaccine delivery. Nanotechnology-based vaccines, called nanovaccines, address the delivery limitations through co-delivery of antigens and adjuvants into lymphoid organs and APCs and their intracellular release, leading to cross-presentation of antigens and induction of potent anti-tumor immune responses. Although the nanovaccines, either as encapsulating agents or biomimetic nanoparticles, exert the desired anti-tumor activities, there is evidence that the mixing formulation to form nanocomplexes between antigens and adjuvants based on the electrostatic interactions provokes high levels of immune responses owing to Ags' availability and faster release. Here, we summarized the various platforms for developing cancer vaccines and the advantages of using delivery systems. The cancer nanovaccines, including nanoparticle-based and biomimetic-based nanovaccines, are discussed in detail. Finally, we focused on the nanocomplexes formation between antigens and adjuvants as promising cancer nanovaccine platforms.
    MeSH term(s) Humans ; Cancer Vaccines/therapeutic use ; Adjuvants, Immunologic ; Neoplasms/therapy ; Antigens, Neoplasm ; Nanoparticles ; Immunotherapy
    Chemical Substances Cancer Vaccines ; Adjuvants, Immunologic ; Antigens, Neoplasm
    Language English
    Publishing date 2023-02-24
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2043785-7
    ISSN 1878-1705 ; 1567-5769
    ISSN (online) 1878-1705
    ISSN 1567-5769
    DOI 10.1016/j.intimp.2023.109887
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    Zs.A 5408: Show issues Location:
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  5. Article ; Online: Immunotherapy for Breast Cancer Treatment.

    Simonian, Miganoosh / Haji Ghaffari, Mozhan / Negahdari, Babak

    Iranian biomedical journal

    2021  Volume 25, Issue 3, Page(s) 140–156

    Abstract: Breast cancer, as a heterogeneous disease, includes a wide range of pathological and clinical behaviors. Current treatment protocols, including radiotherapy, chemotherapy, and hormone replacement therapy, are mainly associated with poor response and high ...

    Abstract Breast cancer, as a heterogeneous disease, includes a wide range of pathological and clinical behaviors. Current treatment protocols, including radiotherapy, chemotherapy, and hormone replacement therapy, are mainly associated with poor response and high rate of recurrence. Therefore, more efforts are needed to develop alternative therapies for this type of cancer. Immunotherapy, as a novel strategy in cancer treatment, has a potential in treating breast cancer patients. Although breast cancer has long been considered problematic to treat with immunotherapy, as it is immunologically "cold," numerous newer preclinical and clinical reports now recommend that immunotherapy has the capability to treat breast cancer patients. In this review, we highlight the different immunotherapy strategies in breast cancer treatment.
    Language English
    Publishing date 2021-03-08
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2489282-8
    ISSN 2008-823X ; 1028-852X
    ISSN (online) 2008-823X
    ISSN 1028-852X
    DOI 10.29252/ibj.25.3.140
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    Zs.A 6776: Show issues Location:
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  6. Article ; Online: Screening of candidate genes associated with high titer production of oncolytic measles virus based on systems biology approach.

    Rastegarpanah, Malihe / Azadmanesh, Kayhan / Negahdari, Babak / Asgari, Yazdan / Mazloomi, Mohammadali

    Virus genes

    2022  Volume 58, Issue 4, Page(s) 270–283

    Abstract: The number of viral particles required for oncolytic activity of measles virus (MV) can be more than a million times greater than the reported amount for vaccination. The aim of the current study is to find potential genes and signaling pathways that may ...

    Abstract The number of viral particles required for oncolytic activity of measles virus (MV) can be more than a million times greater than the reported amount for vaccination. The aim of the current study is to find potential genes and signaling pathways that may be involved in the high-titer production of MV. In this study, a systems biology approach was considered including collection of gene expression profiles from the Gene Expression Omnibus (GEO) database, obtaining differentially expressed genes (DEGs), performing gene ontology, functional enrichment analyses, and topological analyses on the protein-protein interaction (PPI) network. Then, to validate the in-silico data, total RNA was isolated from five cell lines, and full-length cDNA from template RNA was synthesized. Subsequently, quantitative reverse transcription-PCR (RT-qPCR) was employed. We identified five hub genes, including RAC1, HSP90AA1, DNM1, LTBP1, and FSTL1 associated with the enhancement in MV titer. Pathway analysis indicated enrichment in PI3K-Akt signaling pathway, axon guidance, proteoglycans in cancer, regulation of actin cytoskeleton, focal adhesion, and calcium signaling pathways. Upon verification by RT-qPCR, the relative expression of candidate genes was generally consistent with our bioinformatics analysis. Hub genes and signaling pathways may be involved in understanding the pathological mechanisms by which measles virus manipulates host factors in order to facilitate its replication. RAC1, HSP90AA1, DNM1, LTBP1, and FSTL1 genes, in combination with genetic engineering techniques, will allow the direct design of high-throughput cell lines to answer the required amounts for the oncolytic activity of MV.
    MeSH term(s) Computational Biology/methods ; Follistatin-Related Proteins/genetics ; Gene Expression Profiling/methods ; Gene Regulatory Networks/genetics ; Measles virus/genetics ; Oncolytic Viruses/genetics ; Phosphatidylinositol 3-Kinases/genetics ; Protein Interaction Maps/genetics ; RNA ; Systems Biology
    Chemical Substances Follistatin-Related Proteins ; RNA (63231-63-0)
    Language English
    Publishing date 2022-04-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639496-6
    ISSN 1572-994X ; 0920-8569
    ISSN (online) 1572-994X
    ISSN 0920-8569
    DOI 10.1007/s11262-022-01902-y
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    Zs.A 2397: Show issues Location:
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  7. Article ; Online: VEGFR2 Mimicking Peptide Inhibits the Proliferation of Human Umbilical Vein Endothelial Cells (Huvecs) by Blocking VEGF.

    Ghasemali, Samaneh / Barzegar, Abolfazl / Farajnia, Safar / Rahmati, Mohammad / Negahdari, Babak / Etemadi, Ali / Nazari, Atefeh

    Anti-cancer agents in medicinal chemistry

    2023  Volume 23, Issue 14, Page(s) 1678–1688

    Abstract: Introduction: A variety of key human physiological processes rely on angiogenesis, ranging from reproduction and fetal growth to wound healing and tissue repair. Furthermore, this process significantly contributes to tumor progression, invasion, and ... ...

    Abstract Introduction: A variety of key human physiological processes rely on angiogenesis, ranging from reproduction and fetal growth to wound healing and tissue repair. Furthermore, this process significantly contributes to tumor progression, invasion, and metastasis. As the strongest inducer of angiogenesis, Vascular Endothelial Growth Factor (VEGF) and its receptor (VEGFR) are targets of therapeutic research for blocking pathological angiogenesis.
    Objective: Preventing the interaction between VEGF and VEGFR2 by a peptide is a promising strategy for developing antiangiogenic drug candidates. This study was aimed at designing and evaluating VEGF-targeting peptides using
    Methods: The VEGF binding site of VEGFR2 was considered a basis for peptide design. The interaction of VEGF and all three peptides derived from VEGFR2 were analyzed using ClusPro tools. In a complex with VEGF, the peptide with a higher docking score was evaluated to confirm its stability using molecular dynamics (MD) simulation. The gene coding for the selected peptide was cloned and expressed in
    Results: Among three peptides, the peptide with the best docking pose and the highest affinity for VEGF was selected for further studies. Then the stability of the peptide was confirmed over the 100 ns MD simulation. After
    Conclusion: In summary, the selected peptide demonstrated a promising inhibitory effect on human umbilical vein endothelial cells that could be a valuable anti-angiogenic candidate for further assessment. Additionally, these
    MeSH term(s) Humans ; Vascular Endothelial Growth Factor A/metabolism ; Human Umbilical Vein Endothelial Cells ; Escherichia coli/metabolism ; Cell Proliferation ; Vascular Endothelial Growth Factors/metabolism ; Peptides/pharmacology ; Angiogenesis Inhibitors/pharmacology ; Angiogenesis Inhibitors/chemistry ; Vascular Endothelial Growth Factor Receptor-2 ; Cell Movement
    Chemical Substances Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; Peptides ; Angiogenesis Inhibitors ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
    Language English
    Publishing date 2023-05-14
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2217610-X
    ISSN 1875-5992 ; 1871-5206
    ISSN (online) 1875-5992
    ISSN 1871-5206
    DOI 10.2174/1871520623666230517141144
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    Zs.A 5583: Show issues Location:
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  8. Article ; Online: Mixed oral biofilms are controlled by the interspecies interactions of Fusobacterium nucleatum.

    Valadbeigi, Hassan / Khoshnood, Saeed / Negahdari, Babak / Maleki, Abbas / Mohammadinejat, Medya / Haddadi, Mohammad Hossein

    Oral diseases

    2023  

    Abstract: Background: Fusobacterium nucleatum (F. nucleatum) is an integral component of supra- and subgingival biofilms, especially more prevalent in subgingival areas during both periodontal health and disease.: Aims: In this review, we explore the physical, ...

    Abstract Background: Fusobacterium nucleatum (F. nucleatum) is an integral component of supra- and subgingival biofilms, especially more prevalent in subgingival areas during both periodontal health and disease.
    Aims: In this review, we explore the physical, metabolic, and genetic interactions that influence the role of F. nucleatum in the formation of mixed oral biofilms. The role of F. nucleatum in antibiotic resistance in oral biofilms was discussed and some therapeutic strategies were proposed.
    Methods: PubMed, Scopus, Google Scholar, and the Web of Science were extensively searched for English-language reports.
    Results: F. nucleatum-derived proteins such as RadD, Fap2, FomA, and CmpA are involved in direct interactions contributing to biofilm formation, while autoinducer-2 and putrescine are involved in metabolic interactions. Both groups are essential for the formation and persistence of oral biofilms. This study highlights the clinical relevance of targeted interactions of F. nucleatum in supra- and subgingival oral biofilms.
    Conclusions: By focusing on these interactions, researchers and clinicians can develop more effective strategies to prevent biofilm-related disease and reduce the spread of antibiotic resistance. Further research in this area is warranted to explore the potential therapeutic interventions that can be derived from understanding the interactions of F. nucleatum in oral biofilm dynamics.
    Language English
    Publishing date 2023-11-27
    Publishing country Denmark
    Document type Journal Article ; Review
    ZDB-ID 1290529-x
    ISSN 1601-0825 ; 1354-523X
    ISSN (online) 1601-0825
    ISSN 1354-523X
    DOI 10.1111/odi.14822
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    Zs.A 4427: Show issues Location:
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  9. Article ; Online: Nanotechnology and nano-sized tools: Newer approaches to circumvent oncolytic adenovirus limitations.

    Shirazi, Maryam Mashhadi Abolghasem / Saedi, Tayebeh Azam / Moghaddam, Zahra Samadi / Nemati, Mahnaz / Shiri, Reza / Negahdari, Babak / Goradel, Nasser Hashemi

    Pharmacology & therapeutics

    2024  Volume 256, Page(s) 108611

    Abstract: Oncolytic adenoviruses (OAds), engineered Ads preferentially infect and lyse tumor cells, have attracted remarkable attention as immunotherapy weapons for the treatment of various malignancies. Despite hopeful successes in preclinical investigations and ... ...

    Abstract Oncolytic adenoviruses (OAds), engineered Ads preferentially infect and lyse tumor cells, have attracted remarkable attention as immunotherapy weapons for the treatment of various malignancies. Despite hopeful successes in preclinical investigations and translation into clinical phases, they face some challenges that thwart their therapeutic effectiveness, including low infectivity of cancer cells, liver sequestration, pre-existing neutralizing antibodies, physical barriers to the spread of Ads, and immunosuppressive TME. Nanotechnology and nano-sized tools provide several advantages to overcome these limitations of OAds. Nano-sized tools could improve the therapeutic efficacy of OAds by enhancing infectivity and cellular uptake, targeting and protecting from pre-existing immune responses, masking and preventing liver tropism, and co-delivery with other therapeutic agents. Herein, we reviewed the constructs of various OAds and their application in clinical trials, as well as the limitations they have faced. Furthermore, we emphasized the potential applications of nanotechnology to solve the constraints of OAds to improve their anti-tumor activities.
    MeSH term(s) Humans ; Oncolytic Virotherapy ; Oncolytic Viruses ; Adenoviridae ; Neoplasms/therapy ; Nanotechnology
    Language English
    Publishing date 2024-02-20
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 194735-7
    ISSN 1879-016X ; 0163-7258
    ISSN (online) 1879-016X
    ISSN 0163-7258
    DOI 10.1016/j.pharmthera.2024.108611
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  10. Article: Screening of candidate genes associated with high titer production of oncolytic measles virus based on systems biology approach

    Rastegarpanah, Malihe / Azadmanesh, Kayhan / Negahdari, Babak / Asgari, Yazdan / Mazloomi, Mohammadali

    Virus genes. 2022 Aug., v. 58, no. 4

    2022  

    Abstract: The number of viral particles required for oncolytic activity of measles virus (MV) can be more than a million times greater than the reported amount for vaccination. The aim of the current study is to find potential genes and signaling pathways that may ...

    Abstract The number of viral particles required for oncolytic activity of measles virus (MV) can be more than a million times greater than the reported amount for vaccination. The aim of the current study is to find potential genes and signaling pathways that may be involved in the high-titer production of MV. In this study, a systems biology approach was considered including collection of gene expression profiles from the Gene Expression Omnibus (GEO) database, obtaining differentially expressed genes (DEGs), performing gene ontology, functional enrichment analyses, and topological analyses on the protein–protein interaction (PPI) network. Then, to validate the in-silico data, total RNA was isolated from five cell lines, and full-length cDNA from template RNA was synthesized. Subsequently, quantitative reverse transcription-PCR (RT-qPCR) was employed. We identified five hub genes, including RAC1, HSP90AA1, DNM1, LTBP1, and FSTL1 associated with the enhancement in MV titer. Pathway analysis indicated enrichment in PI3K-Akt signaling pathway, axon guidance, proteoglycans in cancer, regulation of actin cytoskeleton, focal adhesion, and calcium signaling pathways. Upon verification by RT-qPCR, the relative expression of candidate genes was generally consistent with our bioinformatics analysis. Hub genes and signaling pathways may be involved in understanding the pathological mechanisms by which measles virus manipulates host factors in order to facilitate its replication. RAC1, HSP90AA1, DNM1, LTBP1, and FSTL1 genes, in combination with genetic engineering techniques, will allow the direct design of high-throughput cell lines to answer the required amounts for the oncolytic activity of MV.
    Keywords Measles morbillivirus ; RNA ; axons ; calcium ; computer simulation ; focal adhesions ; gene expression ; gene expression regulation ; gene ontology ; microfilaments ; protein-protein interactions ; proteoglycans ; reverse transcriptase polymerase chain reaction ; topology ; vaccination ; viruses
    Language English
    Dates of publication 2022-08
    Size p. 270-283.
    Publishing place Springer US
    Document type Article
    ZDB-ID 639496-6
    ISSN 1572-994X ; 0920-8569
    ISSN (online) 1572-994X
    ISSN 0920-8569
    DOI 10.1007/s11262-022-01902-y
    Database NAL-Catalogue (AGRICOLA)

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