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  1. Article ; Online: Association of genetic and epigenetic changes of insulin like growth factor binding protein-1 in Egyptian patients with type 2 diabetes mellitus.

    Hasan, Nehal Salah / Gamal El Dine, Hesham / Kamel, Solaf Ahmed / Hamed, Mona / Youssef, Rasha N / Mahmoud Hassan, Eman / Abdelrahman, Amany Hosny / Musa, Nevine Ibrahim / Ali, Asmaa / Awadallah, Eman

    Diabetes research and clinical practice

    2023  Volume 200, Page(s) 110677

    Abstract: Background: Diabetes is one of the global health threat. Type 2 Diabetes mellitus (T2DM) is associated with life-threatening complications. This work, aimed to study the association between T2DM and IGFBP-1 gene methylation, gene polymorphism and serum ... ...

    Abstract Background: Diabetes is one of the global health threat. Type 2 Diabetes mellitus (T2DM) is associated with life-threatening complications. This work, aimed to study the association between T2DM and IGFBP-1 gene methylation, gene polymorphism and serum levels of IGFBP-1.
    Method: We included 100 subjects with T2DM and 100 control. DNA methylation of IGFBP-1 was analyzed using pyrosequencing, IGFBP-1 gene polymorphism was analyzed using real time polymerase chain reaction and serum level of IGFBP-1 was measured by ELISA.
    Results: There was DNA hyper methylation levels of IGFBP1 gene at each of the six CpG sites in T2DM patients than control (P < 0.001). IGFBP-1 gene polymorphism (rs 2854843) CC pattern was significantly associated with DM, P = 0.002. Also, there was decrease in serum IGFBP-1 in patients with T2DM than control group (P < 0.001).
    Conclusion: We concluded that DNA hyper methylation of IGFBP-1 gene and CC polymorphism (rs 2854843) of IGFBP-1 gene are associated with T2DM in Egyptian patients, also, decrease serum level of IGFBP-1. Further cohort study is recommended with large sample size to detect which one, epigenetic changes or polymorphism of IGFBP-1 gene, is the cause of T2DM or even both.
    MeSH term(s) Humans ; Cohort Studies ; Diabetes Mellitus, Type 2/complications ; DNA ; Egypt ; Epigenesis, Genetic ; Insulin-Like Growth Factor Binding Protein 1/genetics ; Insulin-Like Growth Factor Binding Protein 1/metabolism ; Insulin-Like Growth Factor I/metabolism
    Chemical Substances DNA (9007-49-2) ; Insulin-Like Growth Factor Binding Protein 1 ; Insulin-Like Growth Factor I (67763-96-6)
    Language English
    Publishing date 2023-04-22
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 632523-3
    ISSN 1872-8227 ; 0168-8227
    ISSN (online) 1872-8227
    ISSN 0168-8227
    DOI 10.1016/j.diabres.2023.110677
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  2. Article ; Online: Insulin-like growth factor binding protein 1 DNA methylation in type 2 diabetes

    Sally M. Hafez / Hazem. El-Sayed Abou-youssef / Mona Abdel-Kader Awad / Solaf Ahmed Kamel / Rasha N. Youssef / Suzan Mahrous Elshiekh / Hala Raslan / Nehal Salah

    Egyptian Journal of Medical Human Genetics, Vol 22, Iss 1, Pp 1-

    2021  Volume 8

    Abstract: Abstract Background Type 2 diabetes (T2D) is a complex trait in humans. Several environmental and hereditary factors contribute to the overall pathogenesis of this disease. The association between genes, environment, and T2D was unknown for decades until ...

    Abstract Abstract Background Type 2 diabetes (T2D) is a complex trait in humans. Several environmental and hereditary factors contribute to the overall pathogenesis of this disease. The association between genes, environment, and T2D was unknown for decades until epigenetics was discovered. Epigenetics affects gene transcription, which, in turn, influences organ function. One of the epigenetic regulatory mechanisms is DNA methylation. This mechanism permits modification of gene function without changes in the DNA sequence. There are several risk factors for type 2 diabetes such as harmful intrauterine environment, obesity, poor physical activity, increasing age, a family history of the disease, and an unhealthy diet. All these factors have been proven to influence the DNA methylation sequence in target tissues for insulin resistance in humans. We aimed to evaluate insulin-like growth factor binding protein-1 (IGFBP1) gene methylation levels in T2D. In all, 100 Egyptian individuals were included in this study: 50 patients with T2D versus 50 healthy controls. Genomic DNA was extracted from peripheral blood and IGFBP1 methylation levels were analyzed using pyrosequencing. Results DNA methylation levels in the IGFBP1 gene at each of the six CpG sites were significantly higher in the T2D patients than in the controls at P values of 0.001, 0.002, 0.010, 0.007, 0.014, and 0.001, respectively. Conclusion According to this study, T2D is due to interactions between genetics, epigenetics, and lifestyle. This study also revealed that DNA methylation levels of the IGFBP-1 gene are higher in T2D patients than in healthy control.
    Keywords Epigenetics ; IGFBP1 ; DNA methylation ; Type 2 diabetes mellitus ; Medicine (General) ; R5-920 ; Genetics ; QH426-470
    Subject code 570
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: GLUL rs10911021 polymorphism and risk of coronary artery disease among Egyptian individuals

    Rasha Nazih Yousef / Solaf Ahmed Kamel / Nehal Salah Hasan / Mona Awad / Hesham Gamal / Nevine Ibrahim Musa / Mona Hamed Farag

    Bulletin of the National Research Centre, Vol 43, Iss 1, Pp 1-

    2019  Volume 5

    Abstract: Abstract Background Genome-wide association studies have identified novel genes related to coronary artery disease (CAD). These studies have been replicated in distinct ethnic populations, returning inconsistent results. Our work aimed to study the ... ...

    Abstract Abstract Background Genome-wide association studies have identified novel genes related to coronary artery disease (CAD). These studies have been replicated in distinct ethnic populations, returning inconsistent results. Our work aimed to study the frequency of C and T alleles of GLUL polymorphism genetic variant rs10911021 among Egyptians with coronary artery disease in comparison to apparently healthy subjects. Our study included 420 patients with CAD (180 CAD without T2DM, 240 CAD with T2DM patients) and 200 control subjects. All subjects were genotyped for rs10911021 by real-time polymerase chain reaction. Results For rs10911021, the frequency of (C/T + T/T) genotypes was significantly higher in CAD patients with and without T2DM than in controls (55(45 + 10) % vs. 22(19 + 3) %; p < 0.001) and (50(45 + 5) % vs. 22(19 + 3) %; p < 0.001 respectively). The genotype C/C was the most frequent among the controls (78%). The presence of GLUL polymorphism was associated with 4.4-fold increased risk to develop CAD in diabetic patients (OR = 4.4, 95% CI = (2.2–8.7); p < 0.001) and was associated with 2.3-fold increased risk to develop CAD (OR = 2.3, 95% CI = (1.1–4.6); p = 0.0213). Conclusion In conclusion, among Egyptians, the GLUL polymorphism susceptibility variant rs10911021 is associated with CAD, with and without T2DM.
    Keywords Coronary artery disease ; Single-nucleotide polymorphism ; GLUL ; Type 2 diabetes mellitus ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2019-03-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Peroxisome proliferator-activated receptor-γ polymorphism (rs1801282) is associated with obesity in Egyptian patients with coronary artery disease and type 2 diabetes mellitus.

    Hasan, Nehal Salah / Kamel, Solaf Ahmed / Hamed, Mona / Awadallah, Eman / Rahman, Amany Hosny Abdel / Musa, Nevine Ibrahim / Hussein, Ghada Hussein Sayed

    Journal, genetic engineering & biotechnology

    2017  Volume 15, Issue 2, Page(s) 409–414

    Abstract: Objective: Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) gene is one of the possible genes linking diabetes mellitus (DM) with coronary artery disease (CAD). The aim of this study is to clarify whether PPAR-γ Pro12Ala polymorphism is associated ... ...

    Abstract Objective: Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) gene is one of the possible genes linking diabetes mellitus (DM) with coronary artery disease (CAD). The aim of this study is to clarify whether PPAR-γ Pro12Ala polymorphism is associated with the development of CAD in type 2 diabetic patients and to evaluate PPAR-γ Pro12Ala polymorphism genetic distribution in type 2 DM (T2DM) Egyptian subjects.
    Methods: PPAR-γ Pro12Ala polymorphism was determined by Real-Time PCR in serum of 405 subjects classified into 4 groups; T2DM patients (n = 105), T2DM with CAD (n = 100), CAD patients (n = 100) and healthy controls (n = 100).
    Results: The PPAR-γ Pro12Ala polymorphism was associated significantly with T2DM with CAD (group2) (OR = 3, 95% CI = (1.5-6); p = 0.001). In this study, T2DM with CAD complications carrying the PPAR-γ Pro12Ala polymorphism had higher BMI than those without the PPAR-γ Pro12Ala polymorphism (p < 0.0001). CAD patients carrying PPAR-γ Pro12Ala polymorphism had considerable insulin resistance features. Plasma paraoxanase 1(PON1) level was considerably reduced among our 3 studied groups in comparison to control group (p < 0.001).
    Conclusions: PPAR-γ Pro12Ala polymorphism might represent a novel risk factor for CAD in T2DM.
    Language English
    Publishing date 2017-08-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2637420-1
    ISSN 2090-5920 ; 1687-157X ; 2090-5920
    ISSN (online) 2090-5920
    ISSN 1687-157X ; 2090-5920
    DOI 10.1016/j.jgeb.2017.08.002
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  5. Article ; Online: Original paper The leukemogenic role of (iASPP) in acute leukemia

    Sohier Abdel Almawgood / Nehal Salah Hasan / Mai Yousef

    Archives of Medical Science, Vol 3, Iss 2, Pp 102-

    2007  Volume 107

    Abstract: Introduction: The ASPP family (apoptosis – stimulating proteins of p53) comprises three proteins, ASPP1, ASPP2 and iASPP, that interact with and modulate the behavior of p53. ASPP1 and ASPP2 enhance the ability of p53 to induce apoptosis by causing p53 ... ...

    Abstract Introduction: The ASPP family (apoptosis – stimulating proteins of p53) comprises three proteins, ASPP1, ASPP2 and iASPP, that interact with and modulate the behavior of p53. ASPP1 and ASPP2 enhance the ability of p53 to induce apoptosis by causing p53 to up regulate the expression of proapoptotic genes specifically rather than genes involved in cell cycle arrest. Inhibitory member of the ASPP family (iASPP) acts as inhibitor for the p53, it was originally identified as a nuclear protein that interacts with and inhibits NFkb p56 RelA and inhibits p53-mediated cell death as well. Material and methods: To examine the role of iASPP in acute leukemic patients, we analyzed iASPP mRNA expression in acute leukemia by semi quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Results: The results showed that the median level of iASPP in acute lymphoblastic leukemia and acute myeloid leukemia patients was significantly higher than those in cells from normal donors (p=0.04). The expression of iASPP in ALL and AML patients was not associated with age, gender, hemoglobin, platelets; blasts count in bone marrow, treatment outcome but was associated with blasts count in peripheral blood and total leucocytic count in AL patients. Conclusions: The results of the present study suggest that iASPP may play a role in leukemogenesis and/or disease progression of acute leukemia.
    Keywords acute leukemia ; ALL ; AML ; iASPP ; p53 ; apoptosis ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2007-06-01T00:00:00Z
    Publisher Termedia Publishing House
    Document type Article ; Online
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  6. Article: Peroxisome proliferator-activated receptor-γ polymorphism (rs1801282) is associated with obesity in Egyptian patients with coronary artery disease and type 2 diabetes mellitus

    Hasan, Nehal Salah / Kamel, Solaf Ahmed / Hamed, Mona / Awadallah, Eman / Rahman, Amany Hosny Abdel / Musa, Nevine Ibrahim / Hussein, Ghada Hussein Sayed

    Journal of Genetic Engineering and Biotechnology (Print). 2017 Dec., v. 15, no. 2

    2017  

    Abstract: Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) gene is one of the possible genes linking diabetes mellitus (DM) with coronary artery disease (CAD). The aim of this study is to clarify whether PPAR-γ Pro12Ala polymorphism is associated with the ... ...

    Abstract Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ) gene is one of the possible genes linking diabetes mellitus (DM) with coronary artery disease (CAD). The aim of this study is to clarify whether PPAR-γ Pro12Ala polymorphism is associated with the development of CAD in type 2 diabetic patients and to evaluate PPAR-γ Pro12Ala polymorphism genetic distribution in type 2DM (T2DM) Egyptian subjects.PPAR-γ Pro12Ala polymorphism was determined by Real-Time PCR in serum of 405 subjects classified into 4 groups; T2DM patients (n=105), T2DM with CAD (n=100), CAD patients (n=100) and healthy controls (n=100).The PPAR-γ Pro12Ala polymorphism was associated significantly with T2DM with CAD (group2) (OR=3, 95% CI=(1.5–6); p=0.001). In this study, T2DM with CAD complications carrying the PPAR-γ Pro12Ala polymorphism had higher BMI than those without the PPAR-γ Pro12Ala polymorphism (p<0.0001). CAD patients carrying PPAR-γ Pro12Ala polymorphism had considerable insulin resistance features. Plasma paraoxanase 1(PON1) level was considerably reduced among our 3 studied groups in comparison to control group (p<0.001).PPAR-γ Pro12Ala polymorphism might represent a novel risk factor for CAD in T2DM.
    Keywords blood serum ; body mass index ; coronary artery disease ; genes ; insulin resistance ; noninsulin-dependent diabetes mellitus ; obesity ; patients ; quantitative polymerase chain reaction ; risk factors ; Egypt
    Language English
    Dates of publication 2017-12
    Size p. 409-414.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2637420-1
    ISSN 1687-157X
    ISSN 1687-157X
    DOI 10.1016/j.jgeb.2017.08.002
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Red cell alloimmunization and autoantibodies in Egyptian transfusion-dependent thalassaemia patients.

    Ahmed, Azza Mohamed / Hasan, Nehal Salah / Ragab, Shadia Hassan / Habib, Sonia Adolf / Emara, Nahed Abdelmonem / Aly, Azza Ahmed

    Archives of medical science : AMS

    2010  Volume 6, Issue 4, Page(s) 592–598

    Abstract: Introduction: The objective of this study was to explore the frequency of red cell alloantibodies and autoantibodies among β-thalassaemia patients who received regular transfusions.: Material and methods: This study included 501 patients with β- ... ...

    Abstract Introduction: The objective of this study was to explore the frequency of red cell alloantibodies and autoantibodies among β-thalassaemia patients who received regular transfusions.
    Material and methods: This study included 501 patients with β-thalassaemia. This work planned to study the presence of alloantibodies and autoantibodies to different red cell antigens in multitransfused thalassaemia patients using the ID. Card micro typing system.
    Results: Of a total of 501 β-thalassaemia patients included in the study, 11.3% of patients developed alloantibodies; 9.7% of these alloantibodies were clinically significant. The most common alloantibodies were anti-K, anti-E and anti-C. The rate of incidence of these alloantibodies was 3.9%, 3.3% and 1.7% respectively. Autoantibodies occurred in 28.8% of the patients and 22.1% of these antibodies were typed IgG. There was a significant association between splenectomy with alloimmunization and autoantibody formation (p = 0.03, p = 0.001 respectively). There was no significant association between alloantibody, autoantibody formation and number of transfused packed red cells.
    Conclusions: Alloimmunization to minor erythrocyte antigens and erythrocyte autoantibodies of variable clinical significance are frequent findings in transfused β-thalassaemia patients. There is an association between absence of the spleen and the presence of alloimmunization and autoantibody formation.
    Language English
    Publishing date 2010-09-07
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2203781-0
    ISSN 1896-9151 ; 1896-9151
    ISSN (online) 1896-9151
    ISSN 1896-9151
    DOI 10.5114/aoms.2010.14473
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