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  1. Article ; Online: The Medicinal Effects of Different Solvent Extracts of Pyracantha Coccinea Roem. Fruits

    Neslihan Tekin KARACAER

    Bezmiâlem Science, Vol 11, Iss 1, Pp 23-

    Heavy Metal Content, Antioxidant, and Antimicrobial Properties

    2023  Volume 31

    Abstract: Objective:The current investigation has been conducted to assess the total flavonoid content, total phenolic content, heavy metal composition, and antioxidant and antimicrobial activities of Pyracantha coccinea Roem. fruit extracts prepared with ... ...

    Abstract Objective:The current investigation has been conducted to assess the total flavonoid content, total phenolic content, heavy metal composition, and antioxidant and antimicrobial activities of Pyracantha coccinea Roem. fruit extracts prepared with different solvents.Methods:Ethanol, diethyl ether, and hot water extraction were used as solvents to prepare the extract of Pyracantha coccinea Roem. fruit. Total phenolic ingredient was assessed by Folin- Ciocalteu assay, and the total ingredient of flavonoids was measured spectrophotometrically via AlCl3 assay. The antioxidant activities of the extracts were investigated via free radical scavenging assays, DPPH, and ABTS. The fruits were analyzed by Inductively Coupled Plasma/Mass Spectrometer to determine heavy metal content. The antimicrobial activities of the extracts were investigated using agar well diffusion method against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and Candida albicans.Results:It was determined that the total flavonoid ingredient, total phenolic ingredient, ABTS and DPPH activities of the hot water extract were significantly higher than the other fractions. These parameters were found to be significantly higher in ethanol extract compared to ether extract. All extracts exhibited antimicrobial activity against Bacillus cereus and Pseudomonas aeruginosa while the hot water fraction exhibited the highest antibacterial effect against Pseudomonas aeruginosa. It was determined that Cr, Co, Ni, and Cu contents exceeded the toxicity thresholds that might be found in plants.Conclusion:These results suggest that Pyracantha coccinea Roem. fruit may be considered as a natural source of antioxidants and antimicrobial agents.
    Keywords abts ; antimicrobial activity ; antioxidant activity ; dpph ; pyracantha coccinea roem ; Medicine (General) ; R5-920
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher Galenos Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: In vitro AND in silico EVALUATION OF THYMOQUINONE AS POTENTIAL ANTICANCER AGENT IN HUMAN ACUTE MYELOID LEUKEMIA HL-60 CELLS

    Neslihan Tekin Karacaer

    Trakya University Journal of Natural Sciences, Vol 23, Iss 1, Pp 53-

    2022  Volume 63

    Abstract: This study aims to explore the cytotoxic, apoptotic and autophagic effects of thymoquinone on human acute myeloid leukemia. The cytotoxic effects of thymoquinone were determined with 3-(4, 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) ...

    Abstract This study aims to explore the cytotoxic, apoptotic and autophagic effects of thymoquinone on human acute myeloid leukemia. The cytotoxic effects of thymoquinone were determined with 3-(4, 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) tests. B-cell lymphoma 2 associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), caspase 3, mammalian target of rapamycin (mTOR), phosphatidylinositol-3-kinase (PI3K), and protein kinase B (AKT) gene expression analyzes were studied with quantitative real-time polymerase chain reaction (qRT-PCR). AutoDock Tools 4.2 software was applied to research the potential binding of thymoquinone in the active sites of Bax, Bcl-2, caspase 3, mTOR, PI3K, and AKT proteins. Thymoquinone caused a cytotoxic effect on HL-60 cells (Human leukemia cell line) with a value of 16.35 µM. Bcl-2 expression was decreased in all concentrations applied compared to the control. A decrease in caspase 3 expression level was detected in the cells treated with 10 µM, 15 µM, and 25 µM thymoquinone compared to the control. Thymoquinone induced an important decrease in mTOR and PI3K expressions compared to the control at all doses, while AKT decreased at a dose of 15 µM. The docking outcomes showed that thymoquinone interacts with the active site amino acids of apoptotic and autophagic proteins via hydrophobic interactions and hydrogen bonding. The present findings suggest that thymoquinone can stimulate autophagy by prevention of PI3K/AKT/mTOR pathway in HL-60 cells and may become a new target for the therapy of acute myeloid leukemia.
    Keywords aml ; apoptosis ; autophagy ; molecular docking ; pi3k/akt/mtor ; thymoquinone ; Science (General) ; Q1-390
    Subject code 500
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher Trakya University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Apoptotic Effect of Bortezomib on Pancreatic Islet Cells in STZ-induced Diabetic Rats

    Çiğdem Ekin / Neslihan Tekin Karacaer / Mehtap Tarhan Karaoğlan / İbrahim Örün / Kamile Öztürk

    Makara Journal of Health Research, Vol 26, Iss 3, Pp 197-

    2022  Volume 203

    Abstract: Background: This study aimed to investigate the possible apoptotic role of bortezomib (BMZ) on pancreatic islets of streptozotocin (STZ)-induced diabetic rats. Methods: Sprague-Dawley rats were divided into groups that were administered BMZ alone or in ... ...

    Abstract Background: This study aimed to investigate the possible apoptotic role of bortezomib (BMZ) on pancreatic islets of streptozotocin (STZ)-induced diabetic rats. Methods: Sprague-Dawley rats were divided into groups that were administered BMZ alone or in combination with STZ. To evaluate the effect of BMZ on the development of diabetes, blood glucose levels were measured regularly in the animals. Islet cell viability was determined by staining the islets with fluorescein diacetate and propidium iodide. Expression of the Bcl-2 and bax genes was determined in islet cells by quantitative real-time polymerase chain reaction. Results: Administering STZ-induced hyperglycemia in the rats reduced the viability of islet cells and the bcl-2/bax ratio. In the group administered BMZ alone, the bcl-2/bax gene expression rate in islets increased significantly compared to the control group. BMZ co-administered with STZ significantly increased islet cell viability and the bcl-2/bax ratio compared to the diabetic group. Conclusions: This study demonstrates that BMZ may protect pancreatic islet cells from apoptosis by increasing islet viability and upregulating the bcl-2/bax gene expression ratio, even though it failed to protect against the destructive effect of STZ.
    Keywords apoptosis ; bortezomib ; pancreatic islets ; rats ; type 1 diabetes ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher Universitas Indonesia
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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