LIVIVO - Search results -

Search results

Result 1 - 10 of total 124

Search options

Article ; Online: Isolation protocols and mitochondrial content for plasma extracellular vesicles

Nguyễn, Ái / Turko, Illarion V.

Anal Bioanal Chem. 2023 Mar., v. 415, no. 7 p.1299-1304

2023

Abstract: Mitochondrial content has been reported outside of cells either within extracellular vesicles (EVs) or as free mitochondria. Mitochondrial EVs can potentially play multiple physiological and pathophysiological roles. To understand their functions, ... ...

Abstract	Mitochondrial content has been reported outside of cells either within extracellular vesicles (EVs) or as free mitochondria. Mitochondrial EVs can potentially play multiple physiological and pathophysiological roles. To understand their functions, isolation protocols to separate mitochondrial EVs from other mitochondrial content need to be established. In the present work, we use a multiple reaction monitoring assay with isotope labeled internal standards to quantify 11 mitochondrial, 6 plasma membrane-specific, 4 endosomal membrane-specific, and 2 soluble proteins to evaluate the efficiency of chromatographic isolation of mitochondrial EVs. The isolation protocol includes ultracentrifugation, size exclusion chromatography, and chromatography on immobilized heparin. All protein concentrations were normalized to the concentration of ATP synthase alpha subunit to generate a ratio that allows comparison of different samples obtained during the isolation. We have shown that initial samples after ultracentrifugation are contaminated with non-EV mitochondrial content that cannot be separated from EVs using size exclusion chromatography, but can be efficiently separated from EVs on the column with immobilized heparin.
Keywords	H-transporting ATP synthase ; gel chromatography ; heparin ; isotopes ; mitochondria ; ultracentrifugation
Language	English
Dates of publication	2023-03
Size	p. 1299-1304.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ISSN	1618-2642
DOI	10.1007/s00216-022-04465-x
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Isolation protocols and mitochondrial content for plasma extracellular vesicles.

Nguyen, Ai / Turko, Illarion V

Analytical and bioanalytical chemistry

2022 Volume 415, Issue 7, Page(s) 1299–1304

Abstract	Mitochondrial content has been reported outside of cells either within extracellular vesicles (EVs) or as free mitochondria. Mitochondrial EVs can potentially play multiple physiological and pathophysiological roles. To understand their functions, isolation protocols to separate mitochondrial EVs from other mitochondrial content need to be established. In the present work, we use a multiple reaction monitoring assay with isotope labeled internal standards to quantify 11 mitochondrial, 6 plasma membrane-specific, 4 endosomal membrane-specific, and 2 soluble proteins to evaluate the efficiency of chromatographic isolation of mitochondrial EVs. The isolation protocol includes ultracentrifugation, size exclusion chromatography, and chromatography on immobilized heparin. All protein concentrations were normalized to the concentration of ATP synthase alpha subunit to generate a ratio that allows comparison of different samples obtained during the isolation. We have shown that initial samples after ultracentrifugation are contaminated with non-EV mitochondrial content that cannot be separated from EVs using size exclusion chromatography, but can be efficiently separated from EVs on the column with immobilized heparin.
MeSH term(s)	Extracellular Vesicles/chemistry ; Chromatography, Gel ; Mitochondria ; Heparin/analysis ; Ultracentrifugation
Chemical Substances	Heparin (9005-49-6)
Language	English
Publishing date	2022-12-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-022-04465-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 4' 69/97: Show issues
Archiv 3: Show issues
Z 9.4: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Quantification of rare earth elements in Australian and imported rice samples from different origins using ICP-MS.

Imran, Maryam / Nguyen, Ai / Sultanbawa, Yasmina

The Science of the total environment

2023 Volume 895, Page(s) 164865

Abstract: Rare earth elements (REEs) are also known as lanthanides and are comprised of seventeen elements including lanthanum to lutetium in the periodic table. Despite their increased utilization, little attention is given to them as emerging environmental ... ...

Abstract	Rare earth elements (REEs) are also known as lanthanides and are comprised of seventeen elements including lanthanum to lutetium in the periodic table. Despite their increased utilization, little attention is given to them as emerging environmental contaminants and their associated health risks. The concentration of these elements in urban and agronomic soil may trigger bioaccumulation in plants and may enter the food chain. Also, the consumption of fertilizers in agricultural practices on a larger scale is a significant challenge. The REEs enriched fertilizers are a risk factor for contamination in soil and food. However, there is very limited data in the literature regarding the occurrence of these elements in a staple food such as rice. Thus, this study is aimed at quantification of rare earth elements in Australian and imported rice samples from different countries by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The developed analytical method was validated by using two certified reference materials (CRMs) for precision and accuracy. The method was applied for analysing rice samples (including polished, brown, and parboiled) imported from different countries and sourced locally and consumed by the Australian population. The average concentration of REEs in Australian, Thailand and Vietnamese rice samples were quantified as 0.013-2.974 μg/kg, 0.012-3.113 μg/kg, 0.009-0.919 μg/kg respectively and were lower than other countries. The highest average concentrations of REEs were found in Pakistan (0.299-128.2 μg/kg), India (0.063-20.574 μg/kg), and Sri Lankan (0.022-11.522 μg/kg) rice samples imported to Australia. Scandium and yttrium were found in the range of 107.463-85.961 μg/kg. The pattern of Light rare earth elements (LREE) was more abundant than heavy rare earth elements (HREE). This study did not include field experiments to find the translocation factors of REEs from soil to different parts of plant bodies, thus cannot establish the correlation between fertilizers and REEs concentration in rice grains. However, this study presented the general interpretation of REEs quantification in rice grains from different Countries. The outcome of this study includes filling the subsequent knowledge gaps in analysing REEs in rice. This study indicated the need to establish a monitoring program for this type of staple cereals, aiming at promoting public health.
MeSH term(s)	Oryza ; Fertilizers/analysis ; Australia ; Metals, Rare Earth/analysis ; Soil/chemistry ; Edible Grain/chemistry
Chemical Substances	Fertilizers ; Metals, Rare Earth ; Soil
Language	English
Publishing date	2023-06-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.164865
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 949: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Diagnosis and Management of Non-Infectious Uveitis in Pediatric Patients.

Nguyen, Ai Tien / Koné-Paut, Isabelle / Dusser, Perrine

Paediatric drugs

2023 Volume 26, Issue 1, Page(s) 31–47

Abstract: Uveitis in children accounts for 5-10% of all cases. The causes vary considerably. Classically, uveitis is distinguished according to its infectious or inflammatory origin and whether it is part of a systemic disease or represents an isolated ocular ... ...

Abstract	Uveitis in children accounts for 5-10% of all cases. The causes vary considerably. Classically, uveitis is distinguished according to its infectious or inflammatory origin and whether it is part of a systemic disease or represents an isolated ocular disease. It is important to highlight the specificity of certain etiologies among children such as juvenile idiopathic arthritis. The development of visual function can potentially be hindered by amblyopia (children aged < 7 years), in addition to the usual complications (synechiae, macular edema) seen in adult patients. Moreover, the presentation of uveitis in children is often "silent" with few warning signs and few functional complaints from young children, which frequently leads to a substantial diagnostic delay. The diagnostic approach is guided by the presentation of the uveitis, which can be characterized by its location, and corresponds to the initial and main site of intraocular inflammation; its presentation, whether acute or chronic, granulomatous or not; and the response to treatment. Pediatricians have an important role to play and must be aware of the various presentations and etiologies of uveitis in children. Juvenile idiopathic arthritis is the most common etiology of pediatric non-infectious uveitis, but other causes must be recognized. Promptly initiated treatment before complications arise requires early diagnosis, recognition, and treatment. Any dependence on prolonged local corticosteroid therapy justifies discussing the introduction of a corticosteroid-sparing treatment considering the risk to develop corticoid-induced glaucoma and cataracts. Systemic corticosteroid therapy can be required for urgent control of inflammation in the case of severe uveitis. Long-lasting immunosuppressive treatment and biotherapies are most often prescribed at the same time to reinforce treatment efficacy and to prevent relapse and corticosteroid dependency. We review the different causes of uveitis, excluding infection, and the diagnostic and therapeutic management aimed at limiting the risk of irreversible sequelae.
MeSH term(s)	Child ; Child, Preschool ; Humans ; Adrenal Cortex Hormones/therapeutic use ; Arthritis, Juvenile/complications ; Arthritis, Juvenile/diagnosis ; Arthritis, Juvenile/drug therapy ; Delayed Diagnosis ; Inflammation/complications ; Uveitis/diagnosis ; Uveitis/drug therapy ; Uveitis/etiology
Chemical Substances	Adrenal Cortex Hormones
Language	English
Publishing date	2023-10-04
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	1492748-2
ISSN	1179-2019 ; 1174-5878
ISSN (online)	1179-2019
ISSN	1174-5878
DOI	10.1007/s40272-023-00596-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 5205: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Pore Restructuring of Peptide Frameworks by Mutations at Distal Packing Residues.

Heinz-Kunert, Sherrie L / Pandya, Ashma / Dang, Viet Thuc / Oktawiec, Julia / Nguyen, Andy I

Biomacromolecules

2024 Volume 25, Issue 3, Page(s) 2016–2023

Abstract: Porous framework materials are highly useful for catalysis, adsorption, and separations. Though they are usually made from inorganic and organic building blocks, recently, folded peptides have been utilized for constructing frameworks, opening up an ... ...

Abstract	Porous framework materials are highly useful for catalysis, adsorption, and separations. Though they are usually made from inorganic and organic building blocks, recently, folded peptides have been utilized for constructing frameworks, opening up an enormous structure-space for exploration. These peptides assemble in a metal-free fashion using π-stacking, H-bonding, dispersion forces, and the hydrophobic effect. Manipulation of pore-defining H-bonding residues is known to generate new topologies, but the impact of mutations in the hydrophobic packing region facing away from the pores is less obvious. To explore their effects, we synthesized variants of peptide frameworks with mutations in the hydrophobic packing positions and found by single-crystal X-ray crystallography (SC-XRD) that they induce significant changes to the framework pore structure. These structural changes are driven by a need to maximize van der Waals interactions of the nonpolar groups, which are achieved by various mechanisms including helix twisting, chain flipping, chain offsetting, and desymmetrization. Even subtle changes to the van der Waals interface, such as the introduction of a methyl group or isomeric replacement, result in significant pore restructuring. This study shows that the dispersion interactions upholding a peptide material are a rich area for structural engineering.
MeSH term(s)	Metals/chemistry ; Crystallography, X-Ray ; Peptides/genetics ; Mutation
Chemical Substances	Metals ; Peptides
Language	English
Publishing date	2024-02-16
Publishing country	United States
Document type	Journal Article
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.3c01418
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: The pharmacogenomics of carbamazepine-induced cutaneous adverse drug reaction in the South of Vietnam.

Nguyen, Ai-Hoc / Sukasem, Chonlaphat / Nguyen, Quy Ngoc / Pham, Hong Tham

Frontiers in pharmacology

2023 Volume 14, Page(s) 1217516

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2023-07-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1217516
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mechanistic Aspects of Cobalt-Oxo Cubane Clusters in Oxidation Chemistry.

Amtawong, Jaruwan / Nguyen, Andy I / Tilley, T Don

Journal of the American Chemical Society

2022 Volume 144, Issue 4, Page(s) 1475–1492

Abstract: Cobalt oxides are recognized as one of the most efficient earth-abundant catalysts for challenging oxidation chemistry, with substrates ranging from water to organic compounds. In these oxidations, cobalt-oxo species with formal oxidation states greater ... ...

Abstract	Cobalt oxides are recognized as one of the most efficient earth-abundant catalysts for challenging oxidation chemistry, with substrates ranging from water to organic compounds. In these oxidations, cobalt-oxo species with formal oxidation states greater than 3 are commonly invoked as reactive intermediates. However, there is a dearth of mechanistic information regarding how these high-valent cobalt catalysts operate. This Perspective describes how the study of molecular cobalt oxo clusters, with an emphasis on [Co
Language	English
Publishing date	2022-01-21
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.1c11445
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 55/32: Show issues
Z 7.3: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Root-associated bacteria Bacillus albus and Bacillus proteolyticus promote the growth of peanut seedlings and protect them from the aflatoxigenic Aspergillus flavus CDP2

Trinh, Lai Loi / Le Nguyen, Ai Mi / Aubert-Nguyen, Hoai Huong

Biocatalysis and Agricultural Biotechnology. 2023 Jan., v. 47 p.102582-

2023

Abstract: Three isolates of bacteria NNK24, NDP61, and 0405-02, obtained from the surface and the inside of healthy peanut roots, were considered putative epiphytic and endophytic bacteria of peanut plants. 16S rRNA gene sequencing and BLAST search revealed that ... ...

Abstract	Three isolates of bacteria NNK24, NDP61, and 0405-02, obtained from the surface and the inside of healthy peanut roots, were considered putative epiphytic and endophytic bacteria of peanut plants. 16S rRNA gene sequencing and BLAST search revealed that NNK24, NDP61 belong to Bacillus albus species and 0405-02 belong to Bacillus proteolyticus species. The culture broths of all 3 isolates obtained in the stationary phase showed good antifungal activity, approximately 70%–80% in the in-vitro antagonistic test. Meanwhile, in the in-vivo assay, after 72-hr-incubation of peanut seeds with Aspergillus flavus CDP2 conidia, isolates NNK24 and NDP61 reduced the mold growth from 6.9 log (cfu/g) to 4.5 and 3.9 log (cfu/g), respectively; whereas strain 0405-02 had only a slighter effect on the mold growth. Soaking peanut seeds in bacterial culture broth at 10⁷ cfu/ml for 30 min promoted well seedling growth with the Vigour index (VI) increasing by about 3.6–5.3 times; reduced the Disease severity index (DSI) from 0.59 to 0.18–0.28. At 30 days after planting (DAP) seedlings into the soil, the plant weight and plant length grew approximately by 1.5–1.9 times and 1.4–1.6 times, respectively. Therefore, Bacillus albus NNK24, NDP61 and Bacillus proteolyticus 0405-02 are good candidates for microbial inoculants to promote peanut plant growth and protect them from aflatoxigenic Aspergillus flavus growth in order to develop sustainable agriculture.
Keywords	Aspergillus flavus ; agricultural biotechnology ; antifungal properties ; bacterial culture ; biocatalysis ; conidia ; culture media ; disease severity ; endophytes ; epiphytes ; genes ; peanuts ; seedling growth ; soil ; sustainable agriculture ; vigor ; Bacillus albus ; Bacillus proteolyticus ; Microbial inoculants ; Peanut seeds
Language	English
Dates of publication	2023-01
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	2642052-1
ISSN	1878-8181
ISSN	1878-8181
DOI	10.1016/j.bcab.2022.102582
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Quantitative proteomic analysis for evaluating affinity isolation of extracellular vesicles

Nguyễn, Ái / Wang, Tingting / Turko, Illarion V.

Journal of Proteomics. 2021 Oct., v. 249 p.104359-

2021

Abstract: Absolute quantification with mass spectrometry and isotope labeled internal standards has found broad applications in biomedical research. In the present research, it was used for developing and evaluating a new affinity-based approach to isolate ... ...

Abstract	Absolute quantification with mass spectrometry and isotope labeled internal standards has found broad applications in biomedical research. In the present research, it was used for developing and evaluating a new affinity-based approach to isolate extracellular vesicles (EVs) from human plasma. First, a phage display peptide library was screened against EVs as a bait and absolute quantification of multiple proteins helped to select the best bait available. Then, absolute quantification was used to evaluate the efficiency of affinity chromatography on peptide-Sepharose. In summary, we have demonstrated that peptides with affinity to EVs selected from phage library screening can be valuable ligands for EVs isolation. Extracellular vesicles (EVs) have an important role in intercellular communication for all cell types. This makes EVs a promising new type of therapeutics capable to deliver drugs to specific sites with no off-target side effects. However, their isolation, and correct assignment of their biological function and properties remains an obscure field of research. In this study, we proposed to use MRM quantitation of a pattern of EVs and non-EVs proteins to develop a purification protocol based on affinity peptides selected from phage library screening. MRM quantification of EVs proteins can also help in identifying those that are subpopulation specific markers for further target-specific isolation.
Keywords	DNA libraries ; affinity chromatography ; biomedical research ; cell communication ; humans ; isotopes ; ligands ; mass spectrometry ; peptide libraries ; proteomics ; therapeutics ; Extracellular vesicles ; Targeted proteomics ; QconCATs ; Multiple reaction monitoring ; α-2-MG ; EV ; MRM ; SEC
Language	English
Dates of publication	2021-10
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2021.104359
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article: Modulation of Biophysical Properties of Nucleocapsid Protein in the Mutant Spectrum of SARS-CoV-2.

Nguyen, Ai / Zhao, Huaying / Myagmarsuren, Dulguun / Srinivasan, Sanjana / Wu, Di / Chen, Jiji / Piszczek, Grzegorz / Schuck, Peter

bioRxiv : the preprint server for biology

2024

Abstract: Genetic diversity is a hallmark of RNA viruses and the basis for their evolutionary success. Taking advantage of the uniquely large genomic database of SARS-CoV-2, we examine the impact of mutations across the spectrum of viable amino acid sequences on ... ...

Abstract	Genetic diversity is a hallmark of RNA viruses and the basis for their evolutionary success. Taking advantage of the uniquely large genomic database of SARS-CoV-2, we examine the impact of mutations across the spectrum of viable amino acid sequences on the biophysical phenotypes of the highly expressed and multifunctional nucleocapsid protein. We find variation in the physicochemical parameters of its extended intrinsically disordered regions (IDRs) sufficient to allow local plasticity, but also exhibiting functional constraints that similarly occur in related coronaviruses. In biophysical experiments with several N-protein species carrying mutations associated with major variants, we find that point mutations in the IDRs can have nonlocal impact and modulate thermodynamic stability, secondary structure, protein oligomeric state, particle formation, and liquid-liquid phase separation. In the Omicron variant, distant mutations in different IDRs have compensatory effects in shifting a delicate balance of interactions controlling protein assembly properties, and include the creation of a new protein-protein interaction interface in the N-terminal IDR through the defining P13L mutation. A picture emerges where genetic diversity is accompanied by significant variation in biophysical characteristics of functional N-protein species, in particular in the IDRs.
Language	English
Publishing date	2024-03-22
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.11.21.568093
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

To top

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Inter-library loan at ZB MED