Article ; Online: Donor characteristics and intraoperative total nucleated cell count influence hematopoietic progenitor cell yield of healthy donor bone marrow grafts.
2021 Volume 68, Issue 4, Page(s) e28902
Abstract: Background: Bone marrow graft cell content impacts engraftment potential after allogeneic hematopoietic cell transplantation (alloHCT). Surrogates, such as intraoperative total nucleated cell count (ioTNC), are of unclear utility in predicting final ... ...
Abstract | Background: Bone marrow graft cell content impacts engraftment potential after allogeneic hematopoietic cell transplantation (alloHCT). Surrogates, such as intraoperative total nucleated cell count (ioTNC), are of unclear utility in predicting final graft characteristics. In addition, demographic and clinical factors may influence graft cellular profile and recipient engraftment. Procedure: We retrospectively reviewed marrow harvests at our institution performed between 2009 and 2019. During this time, an ioTNC was measured after 50% of the projected final graft volume was collected. Regression models were used to assess associations between ioTNC (cells/µL) and final graft CD34+ (cells/mL), and between graft and donor characteristics and final graft CD34+ (cells/mL). Results: Fifty-three marrow harvests and donor-recipient pairs were analyzed. Median (range) donor and recipient ages were 13 (0.7-28) years and 9 (0.2-21) years, respectively. The median ratio of donor/recipient weight was 1.225 kg (range 0.31-7.13). Median total volume of harvested marrow was 15.3 mL/kg (range 4.3-20.4) of donor weight and 19.4 mL/kg (range 4.7-87.4) of recipient weight. Median ioTNC was 20 930/µL (range 6600-44310) or 2.1 × 10 Conclusions: ioTNC and certain donor characteristics correlate moderately well with marrow product CD34+ cells/mL, potentially informing donor selection and marrow procurement strategies. |
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MeSH term(s) | Adolescent ; Adult ; Antigens, CD34/analysis ; Bone Marrow Cells/cytology ; Cell Count ; Child ; Child, Preschool ; Donor Selection ; Female ; Graft Survival ; Hematopoietic Stem Cell Transplantation/methods ; Hematopoietic Stem Cells/cytology ; Humans ; Infant ; Male ; Transplantation, Homologous/methods ; Young Adult |
Chemical Substances | Antigens, CD34 |
Language | English |
Publishing date | 2021-01-23 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 2131448-2 |
ISSN | 1545-5017 ; 1545-5009 |
ISSN (online) | 1545-5017 |
ISSN | 1545-5009 |
DOI | 10.1002/pbc.28902 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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