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Article ; Online: Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device.

Nguyen, Suong T / Edo, Ayane / Nagahara, Megumi / Otoi, Takeshige / Taniguchi, Masayasu / Takagi, Mitsuhiro

2023 Volume 260, Page(s) 107386

Abstract: This study aimed to assess the potential of the centrifuge-free commercial device (MIGLIS®) in selecting functional frozen-thawed bovine sperm by migration-sedimentation, its effect on embryo development, and compare the potential with that of ... ...

Abstract	This study aimed to assess the potential of the centrifuge-free commercial device (MIGLIS®) in selecting functional frozen-thawed bovine sperm by migration-sedimentation, its effect on embryo development, and compare the potential with that of centrifugation-based techniques, including washing and Percoll density gradient centrifugation (DGC). In experiment 1, different dilutions (1.5×, 2×, and 3×) of frozen-thawed spermatozoa were assessed to identify the adequate one for the MIGLIS method. In experiment 2, the recovery rates, quality, and reactive oxygen species (ROS) concentrations of the spermatozoa selected using MIGLIS, washing, and Percoll DGC were compared. In experiment 3, the resultant in vitro fertilised embryos from spermatozoa selected using the three methods were evaluated for blastocyst formation rates and intracellular ROS concentrations at the 2-4 cell stage. The intracellular ROS concentrations were investigated using 2', 7'-dichlorodihydrofluorescein diacetate staining. Using the MIGLIS device, significantly more spermatozoa were recovered at 2× dilution compared with the other dilution ratio, but the motility was not affected by the dilution ratio. On the selection of spermatozoa using the three methods, employing MIGLIS decreased the recovery rates. However, the MIGLIS method increased motility, viability, and acrosome integrity rates compared to those in spermatozoa from the other methods. The ROS concentration of spermatozoa in the MIGLIS method was significantly lower than that in the washing method. Nevertheless, blastocyst formation rates were similar among the three methods, but the ROS concentration of early-stage embryos produced using MIGLIS was significantly lower than those produced using Percoll DGC. In conclusion, the MIGLIS method has the potential to select functional, high-quality frozen-thawed bovine spermatozoa.
MeSH term(s)	Male ; Animals ; Cattle ; Semen ; Reactive Oxygen Species ; Cryopreservation/veterinary ; Cryopreservation/methods ; Sperm Motility ; Spermatozoa ; Semen Preservation/veterinary ; Semen Preservation/methods ; Centrifugation/veterinary
Chemical Substances	Percoll (65455-52-9) ; Reactive Oxygen Species
Language	English
Publishing date	2023-11-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	429674-6
ISSN	1873-2232 ; 0378-4320
ISSN (online)	1873-2232
ISSN	0378-4320
DOI	10.1016/j.anireprosci.2023.107386
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Article ; Online: Asymmetric wall ingrowth deposition in Arabidopsis phloem parenchyma transfer cells is tightly associated with sieve elements.

Wei, Xiaoyang / Huang, Yuan / Nguyen, Suong T T / Collings, David A / McCurdy, David W

Journal of experimental botany

2022 Volume 73, Issue 16, Page(s) 5414–5427

Abstract: In Arabidopsis, polarized deposition of wall ingrowths in phloem parenchyma (PP) transfer cells (TCs) occurs adjacent to cells of the sieve element/companion cell (SE/CC) complex. However, the spatial relationships between these different cell types in ... ...

Abstract	In Arabidopsis, polarized deposition of wall ingrowths in phloem parenchyma (PP) transfer cells (TCs) occurs adjacent to cells of the sieve element/companion cell (SE/CC) complex. However, the spatial relationships between these different cell types in minor veins, where phloem loading occurs, are poorly understood. PP TC development and wall ingrowth localization were compared with those of other phloem cells in leaves of Col-0 and the transgenic lines AtSUC2::AtSTP9-GFP (green fluorescent protein) and AtSWEET11::AtSWEET11-GFP that identify CCs and PP cells, respectively. The development of PP TCs in minor veins, indicated by deposition of wall ingrowths, proceeded basipetally in leaves. However, not all PP cells develop wall ingrowths, and higher levels of deposition occur in abaxial- compared with adaxial-positioned PP TCs. Furthermore, the deposition of wall ingrowths was exclusively initiated on and preferentially covered the PP TC/SE interface, rather than the PP TC/CC interface, and only occurred in PP cells that were adjacent to SEs. Collectively, these results demonstrate a tight association between SEs and wall ingrowth deposition in PP TCs and suggest the existence of two subtypes of PP cells in leaf minor veins. Compared with PP cells, PP TCs showed more abundant accumulation of AtSWEET11-GFP, indicating functional differences in phloem loading between PP and PP TCs.
MeSH term(s)	Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Green Fluorescent Proteins/metabolism ; Phloem/metabolism ; Plant Leaves/metabolism
Chemical Substances	Arabidopsis Proteins ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2022-05-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
DOI	10.1093/jxb/erac234
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Article ; Online: Climate dictates microbial community composition and diversity in Australian biological soil crusts (biocrusts).

Chilton, Angela M / Nguyen, Suong T T / Nelson, Tiffanie M / Pearson, Leanne A / Neilan, Brett A

Environmental microbiology

2022 Volume 24, Issue 11, Page(s) 5467–5482

Abstract: The soil surface of drylands can typically be colonized by cyanobacteria and other microbes, forming biological soil crusts or 'biocrusts'. Biocrusts provide critical benefits to ecosystems and are a common component of the largely arid and semi-arid ... ...

Abstract	The soil surface of drylands can typically be colonized by cyanobacteria and other microbes, forming biological soil crusts or 'biocrusts'. Biocrusts provide critical benefits to ecosystems and are a common component of the largely arid and semi-arid Australian continent. Yet, their distribution and the parameters that shape their microbial composition have not been investigated. We present here the first detailed description of Australia's biocrust microbiome assessed from 15 sites across the continent using 16S rRNA sequencing. The most abundant bacterial phyla from all sites were Cyanobacteria, Proteobacteria, Actinobacteria, Chloroflexi and Bacteroidetes. Cyanobacterial communities from northern regions were more diverse and unclassified cyanobacteria were a noticeable feature of northern biocrusts. Segregation between northern and southern regions was largely due to the differential abundance of Microcoleus spp., with M. paludosus dominating in the north and M. vaginatus dominating in the south. The geographical shifts in bacterial composition and diversity were correlated to seasonal temperatures and summer rainfall. Our findings provide an initial reference for sampling strategies to maximize access to bacterial genetic diversity. As hubs for essential ecosystem services, further investigation into biocrusts in arid and semi-arid regions may yield discoveries of genetic mechanisms that combat increases in warming due to climate change.
MeSH term(s)	Soil ; Ecosystem ; Soil Microbiology ; RNA, Ribosomal, 16S/genetics ; Australia ; Microbiota/genetics ; Cyanobacteria/genetics
Chemical Substances	Soil ; RNA, Ribosomal, 16S
Language	English
Publishing date	2022-06-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.16098
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Article ; Online: Chemical Recycling of Thiol Epoxy Thermosets via Light-Driven C-C Bond Cleavage.

Nguyen, Suong T / Fries, Lydia R / Cox, James H / Ma, Yuting / Fors, Brett P / Knowles, Robert R

Journal of the American Chemical Society

2023 Volume 145, Issue 20, Page(s) 11151–11160

Abstract: Epoxy thermosets are high-volume materials that play a central role in a wide range of engineering applications; however, technologies to recycle these polymers remain rare. Here, we present a catalytic, light-driven method that enables chemical ... ...

Abstract	Epoxy thermosets are high-volume materials that play a central role in a wide range of engineering applications; however, technologies to recycle these polymers remain rare. Here, we present a catalytic, light-driven method that enables chemical recycling of industrially relevant thiol epoxy thermosets to their original monomer at ambient temperature. This strategy relies on the proton-coupled electron transfer (PCET) activation of hydroxy groups within the polymer network to generate key alkoxy radicals that promote the fragmentation of the polymer through C-C bond β-scission. The method fully depolymerizes insoluble thiol epoxy thermosets into well-defined mixtures of small-molecule products, which can collectively be converted into the original monomer via a one-step dealkylation process. Notably, this process is selective and efficient even in the presence of other commodity plastics and additives commonly found in commercial applications. These results constitute an important step toward making epoxy thermosets recyclable and more generally exemplify the potential of PCET to offer a more sustainable end-of-life for a diverse array of commercial plastics.
Language	English
Publishing date	2023-05-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.3c00958
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Article ; Online: Histidine-rich protein II nanoparticle delivery of heme iron load drives endothelial inflammation in cerebral malaria.

Nguyen, Suong T / Du, Daniel / Wychrij, Daniel / Cain, Matthew D / Wu, Qingping / Klein, Robyn S / Russo, Ilaria / Goldberg, Daniel E

Proceedings of the National Academy of Sciences of the United States of America

2023 Volume 120, Issue 26, Page(s) e2306318120

Abstract: Histidine-rich protein II (HRPII) is secreted ... ...

Abstract	Histidine-rich protein II (HRPII) is secreted by
MeSH term(s)	Animals ; Mice ; Malaria, Cerebral ; Histidine ; Endothelial Cells ; Inflammation ; Heme ; Hemeproteins ; Malaria, Falciparum ; Iron
Chemical Substances	Histidine (4QD397987E) ; Heme (42VZT0U6YR) ; Hemeproteins ; Iron (E1UOL152H7)
Language	English
Publishing date	2023-06-12
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2306318120
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Article ; Online: Climate dictates microbial community composition and diversity in Australian biological soil crusts (biocrusts)

Chilton, Angela M. / Nguyen, Suong T. T. / Nelson, Tiffanie M. / Pearson, Leanne A. / Neilan, Brett A.

Environmental Microbiology. 2022 Nov., v. 24, no. 11 p.5467-5482

2022

Abstract: The soil surface of drylands can typically be colonized by cyanobacteria and other microbes, forming biological soil crusts or ‘biocrusts’. Biocrusts provide critical benefits to ecosystems and are a common component of the largely arid and semi‐arid ... ...

Abstract	The soil surface of drylands can typically be colonized by cyanobacteria and other microbes, forming biological soil crusts or ‘biocrusts’. Biocrusts provide critical benefits to ecosystems and are a common component of the largely arid and semi‐arid Australian continent. Yet, their distribution and the parameters that shape their microbial composition have not been investigated. We present here the first detailed description of Australia's biocrust microbiome assessed from 15 sites across the continent using 16S rRNA sequencing. The most abundant bacterial phyla from all sites were Cyanobacteria, Proteobacteria, Actinobacteria, Chloroflexi and Bacteroidetes. Cyanobacterial communities from northern regions were more diverse and unclassified cyanobacteria were a noticeable feature of northern biocrusts. Segregation between northern and southern regions was largely due to the differential abundance of Microcoleus spp., with M. paludosus dominating in the north and M. vaginatus dominating in the south. The geographical shifts in bacterial composition and diversity were correlated to seasonal temperatures and summer rainfall. Our findings provide an initial reference for sampling strategies to maximize access to bacterial genetic diversity. As hubs for essential ecosystem services, further investigation into biocrusts in arid and semi‐arid regions may yield discoveries of genetic mechanisms that combat increases in warming due to climate change.
Keywords	Actinobacteria ; Bacteroidetes ; Chloroflexi ; Microcoleus ; Proteobacteria ; arid lands ; biological soil crusts ; climate ; climate change ; community structure ; ecosystems ; genetic variation ; microbial communities ; microbiology ; microbiome ; rain ; summer ; Australia
Language	English
Dates of publication	2022-11
Size	p. 5467-5482.
Publishing place	John Wiley & Sons, Inc.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.16098
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Article: PCET-Enabled Olefin Hydroamidation Reactions with

Nguyen, Suong T / Zhu, Qilei / Knowles, Robert R

ACS catalysis

2019 Volume 9, Issue 5, Page(s) 4502–4507

Abstract: Olefin aminations are important synthetic technologies for the construction of aliphatic C-N bonds. Here we report a catalytic protocol for olefin hydroamidation that proceeds through transient amidyl radical intermediates that are formed via proton- ... ...

Abstract	Olefin aminations are important synthetic technologies for the construction of aliphatic C-N bonds. Here we report a catalytic protocol for olefin hydroamidation that proceeds through transient amidyl radical intermediates that are formed via proton-coupled electron transfer (PCET) activation of the strong N-H bonds in
Language	English
Publishing date	2019-04-17
Publishing country	United States
Document type	Journal Article
ISSN	2155-5435
ISSN	2155-5435
DOI	10.1021/acscatal.9b00966
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Article ; Online: Depolymerization of Hydroxylated Polymers via Light-Driven C-C Bond Cleavage.

Nguyen, Suong T / McLoughlin, Elizabeth A / Cox, James H / Fors, Brett P / Knowles, Robert R

Journal of the American Chemical Society

2021 Volume 143, Issue 31, Page(s) 12268–12277

Abstract: The accumulation of persistent plastic waste in the environment is widely recognized as an ecological crisis. New chemical technologies are necessary both to recycle existing plastic waste streams into high-value chemical feedstocks and to develop next- ... ...

Abstract	The accumulation of persistent plastic waste in the environment is widely recognized as an ecological crisis. New chemical technologies are necessary both to recycle existing plastic waste streams into high-value chemical feedstocks and to develop next-generation materials that are degradable by design. Here, we report a catalytic methodology for the depolymerization of a commercial phenoxy resin and high molecular weight hydroxylated polyolefin derivatives upon visible light irradiation near ambient temperature. Proton-coupled electron transfer (PCET) activation of hydroxyl groups periodically spaced along the polymer backbone furnishes reactive alkoxy radicals that promote chain fragmentation through C-C bond β-scission. The depolymerization produces well-defined and isolable product mixtures that are readily diversified to polycondensation monomers. In addition to controlling depolymerization, the hydroxyl group modulates the thermomechanical properties of these polyolefin derivatives, yielding materials with diverse properties. These results demonstrate a new approach to polymer recycling based on light-driven C-C bond cleavage that has the potential to establish new links within a circular polymer economy and influence the development of new degradable-by-design polyolefin materials.
Language	English
Publishing date	2021-07-31
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.1c05330
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Article: Depolymerization of Hydroxylated Polymers via Light-Driven C–C Bond Cleavage

Nguyen, Suong T. / McLoughlin, Elizabeth A. / Cox, James H. / Fors, Brett P. / Knowles, Robert R.

Journal of the American Chemical Society. 2021 July 31, v. 143, no. 31

2021

Abstract	The accumulation of persistent plastic waste in the environment is widely recognized as an ecological crisis. New chemical technologies are necessary both to recycle existing plastic waste streams into high-value chemical feedstocks and to develop next-generation materials that are degradable by design. Here, we report a catalytic methodology for the depolymerization of a commercial phenoxy resin and high molecular weight hydroxylated polyolefin derivatives upon visible light irradiation near ambient temperature. Proton-coupled electron transfer (PCET) activation of hydroxyl groups periodically spaced along the polymer backbone furnishes reactive alkoxy radicals that promote chain fragmentation through C–C bond β-scission. The depolymerization produces well-defined and isolable product mixtures that are readily diversified to polycondensation monomers. In addition to controlling depolymerization, the hydroxyl group modulates the thermomechanical properties of these polyolefin derivatives, yielding materials with diverse properties. These results demonstrate a new approach to polymer recycling based on light-driven C–C bond cleavage that has the potential to establish new links within a circular polymer economy and influence the development of new degradable-by-design polyolefin materials.
Keywords	ambient temperature ; cleavage (chemistry) ; condensation reactions ; depolymerization ; electron transfer ; feedstocks ; hydroxylation ; irradiation ; light ; molecular weight ; polyolefin ; wastes
Language	English
Dates of publication	2021-0731
Size	p. 12268-12277.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.1c05330
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Article ; Online: Bacterial community structure and metabolic potential in microbialite-forming mats from South Australian saline lakes.

Nguyen, Suong T T / Vardeh, David P / Nelson, Tiffanie M / Pearson, Leanne A / Kinsela, Andrew S / Neilan, Brett A

Geobiology

2022 Volume 20, Issue 4, Page(s) 546–559

Abstract: Microbialites are sedimentary rocks created in association with benthic microorganisms. While they harbour complex microbial communities, Cyanobacteria perform critical roles in sediment stabilisation and accretion. Microbialites have been described from ...

Abstract	Microbialites are sedimentary rocks created in association with benthic microorganisms. While they harbour complex microbial communities, Cyanobacteria perform critical roles in sediment stabilisation and accretion. Microbialites have been described from permanent and ephemeral saline lakes in South Australia; however, the microbial communities that generate and inhabit these biogeological structures have not been studied in detail. To address this knowledge gap, we investigated the composition, diversity and metabolic potential of bacterial communities from different microbialite-forming mats and surrounding sediments in five South Australian saline coastal lakes using 16S rRNA gene sequencing and predictive metagenome analyses. While Proteobacteria and Bacteroidetes were the dominant phyla recovered from the mats and sediments, Cyanobacteria were significantly more abundant in the mat samples. Interestingly, at lower taxonomic levels, the mat communities were vastly different across the five lakes. Comparative analysis of putative mat and sediment metagenomes via PICRUSt2 revealed important metabolic pathways driving the process of carbonate precipitation, including cyanobacterial oxygenic photosynthesis, ureolysis and nitrogen fixation. These pathways were highly conserved across the five examined lakes, although they appeared to be performed by distinct groups of bacterial taxa found in each lake. Stress response, quorum sensing and circadian clock were other important pathways predicted by the in silico metagenome analysis. The enrichment of CRISPR/Cas and phage shock associated genes in these cyanobacteria-rich communities suggests that they may be under selective pressure from viral infection. Together, these results highlight that a very stable ecosystem function is maintained by distinctly different communities in microbialite-forming mats in the five South Australian lakes and reinforce the concept that 'who' is in the community is not as critical as their net metabolic capacity.
MeSH term(s)	Australia ; Cyanobacteria/genetics ; Geologic Sediments/chemistry ; Lakes/microbiology ; Microbiota ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; South Australia
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2022-03-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2113509-5
ISSN	1472-4669 ; 1472-4677
ISSN (online)	1472-4669
ISSN	1472-4677
DOI	10.1111/gbi.12489
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