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Article ; Online: Inoculum effect of Enterobacterales co-expressing OXA-48 and CTX-M on the susceptibility to ceftazidime/avibactam and meropenem.

Mizrahi, A / Chat, L / Danjean, M / Mory, C / Nguyen Van, J C / de Ponfilly, G Péan / Caméléna, F / Le Monnier, A / Bercot, B / Birgy, A / Jacquier, H / Pilmis, B

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2022 Volume 41, Issue 5, Page(s) 853–858

Abstract: Background: The treatment of infections caused by OXA-48/CTX-M-coproducing Enterobacterales may be based on new beta-lactam/beta-lactamase inhibitors, such as ceftazidime/avibactam (CZA), or on high dose of meropenem (MER). However, bacterial density at ...

Abstract	Background: The treatment of infections caused by OXA-48/CTX-M-coproducing Enterobacterales may be based on new beta-lactam/beta-lactamase inhibitors, such as ceftazidime/avibactam (CZA), or on high dose of meropenem (MER). However, bacterial density at the infection site may vary widely, and the inoculum effect of such antimicrobial strategies has never been specifically investigated. To determine if CZA or MER susceptibilities are impacted by high inocula of Enterobacterales co-expressing both enzymes: OXA-48 like and CTX-M. Methods: Determination of an inoculum effect was performed with a standard inoculum of 10 Results: Thirty-nine isolates of ceftazidime-resistant Enterobacterales were included of which 27 (70%) co-expressed OXA-48 + CTX-M-15, 6 (15%) OXA-48 + CTX-M-14, and 6 (15%) OXA-181 + CTX-M-15. The susceptibility to the CZA combination was preserved whatever the inoculum used. Regarding MER, 24 (61.5%) of the isolates were susceptible to MER with the standard inoculum, 19 (48.7%) with a twofold increase, and only 15 (38.5%) with a tenfold increase. Conclusion: We showed that in vitro inoculum effect was observed with meropenem but not with CZA for OXA-48- combined with CTX-M-producing Enterobacterales.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Azabicyclo Compounds/pharmacology ; Ceftazidime/pharmacology ; Drug Combinations ; Enterobacteriaceae/drug effects ; Meropenem/pharmacology ; Microbial Sensitivity Tests ; beta-Lactamase Inhibitors/pharmacology ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Azabicyclo Compounds ; Drug Combinations ; avibactam, ceftazidime drug combination ; beta-Lactamase Inhibitors ; avibactam (7352665165) ; Ceftazidime (9M416Z9QNR) ; beta-Lactamases (EC 3.5.2.6) ; Meropenem (FV9J3JU8B1)
Language	English
Publishing date	2022-03-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-022-04430-7
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Article ; Online: Emergence of Ceftriaxone Resistance during a Case of Pneumococcal Meningitis with Fatal Evolution.

Mizrahi, A / Marvaud, J C / Pilmis, B / Nguyen Van, J C / Couzigou, C / Bruel, C / Engrand, N / Le Monnier, A / Lambert, T

Antimicrobial agents and chemotherapy

2020 Volume 64, Issue 3

Abstract: We report a case of a 62-year-old man treated ... ...

Abstract	We report a case of a 62-year-old man treated for
MeSH term(s)	Ceftriaxone/blood ; Ceftriaxone/pharmacokinetics ; Ceftriaxone/therapeutic use ; Cephalosporins/blood ; Cephalosporins/pharmacokinetics ; Cephalosporins/therapeutic use ; Dexamethasone/blood ; Dexamethasone/pharmacokinetics ; Dexamethasone/therapeutic use ; Humans ; Male ; Meningitis, Pneumococcal/blood ; Meningitis, Pneumococcal/drug therapy ; Meningitis, Pneumococcal/metabolism ; Microbial Sensitivity Tests ; Middle Aged ; Penicillin-Binding Proteins/genetics ; Penicillin-Binding Proteins/metabolism ; Streptococcus pneumoniae/drug effects ; Streptococcus pneumoniae/pathogenicity
Chemical Substances	Cephalosporins ; Penicillin-Binding Proteins ; PBP 2x protein, Streptococcus (128284-03-7) ; Ceftriaxone (75J73V1629) ; Dexamethasone (7S5I7G3JQL)
Language	English
Publishing date	2020-02-21
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.01958-19
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Article: Dynamique de l'excretion virale chez les patients infectes par le SARS-CoV-2

Toulemonde, P. E. / Vidal, B. / Mizrahi, A. / Nguyen Van, J. C. / Lourtet, J. / El Helali, N. / Perniceni, L. / Le Monnier, A. / Couzigou, C.

Medecine et Maladies Infectieuses

Abstract	Declaration de liens d'interets: Les auteurs declarent ne pas avoir de liens d'interets Copyright © 2020
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #832246
Database	COVID19

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Article ; Online: Dynamique de l’excrétion virale chez les patients infectés par le SARS-CoV-2

Toulemonde, P.E. / Vidal, B. / Mizrahi, A. / Nguyen Van, J.C. / Lourtet, J. / El Helali, N. / Perniceni, L. / Le Monnier, A. / Couzigou, C.

Médecine et Maladies Infectieuses

2020 Volume 50, Issue 6, Page(s) S27–S28

Keywords	Infectious Diseases ; covid19
Language	French
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	423536-8
ISSN	0399-077X ; 1166-8237
ISSN	0399-077X ; 1166-8237
DOI	10.1016/j.medmal.2020.06.441
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Article ; Online: Implementation of Alere i Influenza A & B point of care test for the diagnosis of influenza in an ED.

Trabattoni, E / Le, V / Pilmis, B / Pean de Ponfilly, G / Caisso, C / Couzigou, C / Vidal, B / Mizrahi, A / Ganansia, O / Le Monnier, A / Lina, B / Nguyen Van, J C

The American journal of emergency medicine

2017 Volume 36, Issue 6, Page(s) 916–921

Abstract: Study objective: This study aimed to evaluate the impact of implementing rapid point-of-care testing (POCT) with the Alere i Influenza A & B in an emergency department (ED) during an influenza epidemic.: Methods: Direct nasal swabs were prospectively ...

Abstract	Study objective: This study aimed to evaluate the impact of implementing rapid point-of-care testing (POCT) with the Alere i Influenza A & B in an emergency department (ED) during an influenza epidemic. Methods: Direct nasal swabs were prospectively collected following the physical examination of patients aged >18years who presented to the ED of a tertiary hospital in France with influenza-like illness (ILI) symptoms (N=301) between February 1st and March 31st, 2016, which coincided with an influenza epidemic. Laboratory-based testing (standard of care) was used to obtain a diagnosis in February 2016 (pre-POCT cohort) and positive results were confirmed using polymerase chain reaction. The primary endpoint was patient time in the ED. Results: A total of 169 and 132 patients participated in the pre-POCT phase and POCT phase respectively. A significantly higher proportion of patients received a positive diagnosis in the POCT cohort compared with the pre-POCT cohort (31% versus 5.3%, P<0.01). Mean time spent in the ED and hospitalization rate were significantly lower in the POCT cohort (6.06h versus 4.15h, P=0.03, and 44.4% versus 9.7%, P=0.02, respectively). Despite similar rates in the prescription of antibiotics and antiviral therapies, the proportion of patients who were referred for additional tests was significantly lower in the POCT cohort (78.1% versus 62.1%, P=0.003, and 80.5% versus 63.6%, P=0.01, respectively). Conclusions: The Alere i Influenza A & B POCT reduced the length of stay in ED, the hospitalization rates, and the number of additional diagnostic tests compared with standard of care testing.
MeSH term(s)	Adult ; Aged ; DNA, Viral/analysis ; Emergency Service, Hospital ; Female ; France/epidemiology ; Humans ; Incidence ; Influenza A virus/genetics ; Influenza B virus/genetics ; Influenza, Human/diagnosis ; Influenza, Human/epidemiology ; Influenza, Human/virology ; Male ; Middle Aged ; Point-of-Care Testing ; Prospective Studies ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction
Chemical Substances	DNA, Viral
Language	English
Publishing date	2017-10-18
Publishing country	United States
Document type	Journal Article ; Observational Study
ZDB-ID	605890-5
ISSN	1532-8171 ; 0735-6757
ISSN (online)	1532-8171
ISSN	0735-6757
DOI	10.1016/j.ajem.2017.10.046
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Article ; Online: Prospective evaluation of the Alere i Influenza A&B nucleic acid amplification versus Xpert Flu/RSV.

Nguyen Van, J C / Caméléna, F / Dahoun, M / Pilmis, B / Mizrahi, A / Lourtet, J / Behillil, S / Enouf, V / Le Monnier, A

Diagnostic microbiology and infectious disease

2016 Volume 85, Issue 1, Page(s) 19–22

Abstract: The rapid and accurate detection of influenza virus in respiratory specimens is required for optimal management of patients with acute respiratory infections. Because of the variability of the symptoms and the numerous other causes of influenza-like ... ...

Abstract	The rapid and accurate detection of influenza virus in respiratory specimens is required for optimal management of patients with acute respiratory infections. Because of the variability of the symptoms and the numerous other causes of influenza-like illness, the diagnosis of influenza cannot be made on the basis of clinical criteria alone. Thus, rapid influenza diagnostic tests have been developed such as the Alere i Influenza A&B isothermal nucleic acid assay. We prospectively evaluated the performance of the Alere i Influenza A&B assay in comparison with our routine Xpert Flu/RSV assay. Positive samples were subtyped according to the protocol from the National Influenza Center (Paris, France). A total of 96 respiratory nasal swab samples were analyzed: with both methods, 38 were positive and 56 were negative. Samples were prospectively collected from January 20 to April 8, 2015, from patient (86 adult and 10 pediatric patients) presenting with an influenza-like illness through the French influenza season. In comparison with the Xpert Flu/RSV assay, the overall sensitivity and specificity of the Alere i Influenza A&B assay were 95% and 100%, respectively. Our results indicate that the Alere i Influenza A&B assay has a good overall analytical performance and a high degree of concordance with the PCR-based Xpert Flu/RSV assay. The Alere i Influenza A&B isothermal nucleic acid amplification test is a powerful tool for influenza detection due to its high sensitivity and specificity as well as its ability to generate results within 15min.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Influenza A virus/classification ; Influenza A virus/genetics ; Influenza B virus/classification ; Influenza B virus/genetics ; Influenza, Human/diagnosis ; Influenza, Human/immunology ; Influenza, Human/virology ; Male ; Middle Aged ; Nucleic Acid Amplification Techniques/methods ; Nucleic Acid Amplification Techniques/standards ; Reagent Kits, Diagnostic ; Reproducibility of Results ; Sensitivity and Specificity ; Young Adult
Chemical Substances	Reagent Kits, Diagnostic
Language	English
Publishing date	2016-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2015.11.012
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Article ; Online: Infective endocarditis: Clinical presentation, etiology, and early predictors of in-hospital case fatality.

Pilmis, B / Mizrahi, A / Laincer, A / Couzigou, C / El Helali, N / Nguyen Van, J-C / Abassade, P / Cador, R / Le Monnier, A

Medecine et maladies infectieuses

2016 Volume 46, Issue 1, Page(s) 44–48

Abstract: Objective: We aimed to assess the clinical presentation, microbial etiology and outcome of patients presenting with infective endocarditis (IE).: Patients and methods: We conducted a four-year retrospective study including all patients presenting ... ...

Abstract	Objective: We aimed to assess the clinical presentation, microbial etiology and outcome of patients presenting with infective endocarditis (IE). Patients and methods: We conducted a four-year retrospective study including all patients presenting with IE. Results: We included 121 patients in the study. The median age was 74.8years. Most patients had native valve IE (57%). Staphylococcus aureus accounted for 24.8% of all IE. Surgery was indicated for 70 patients (57.9%) but actually performed in only 55 (44.7%). Factors associated with surgery were younger age (P=0.002) and prosthetic valve IE (P=0.001). Risk factors associated with in-hospital mortality were diabetes mellitus (OR=3.17), chronic renal insufficiency (OR=6.62), and surgical indication (OR=3.49). Mortality of patients who underwent surgery was one sixth of that of patients with surgical indication who did not have the surgery (P<0.001).
MeSH term(s)	Aged ; Aged, 80 and over ; Anti-Bacterial Agents/therapeutic use ; Cardiac Surgical Procedures/adverse effects ; Cardiac Surgical Procedures/utilization ; Comorbidity ; Diabetes Mellitus/epidemiology ; Embolism/epidemiology ; Embolism/etiology ; Endocarditis/drug therapy ; Endocarditis/epidemiology ; Endocarditis/etiology ; Endocarditis/surgery ; Female ; France ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation/adverse effects ; Heart Valve Prosthesis Implantation/utilization ; Hospital Mortality ; Humans ; Kidney Failure, Chronic/epidemiology ; Male ; Middle Aged ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Postoperative Complications/therapy ; Prognosis ; Prosthesis-Related Infections/drug therapy ; Prosthesis-Related Infections/epidemiology ; Prosthesis-Related Infections/surgery ; Retrospective Studies
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2016-02
Publishing country	France
Document type	Journal Article
ZDB-ID	423536-8
ISSN	1769-6690 ; 0399-077X ; 1166-8237
ISSN (online)	1769-6690
ISSN	0399-077X ; 1166-8237
DOI	10.1016/j.medmal.2015.12.012
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Article ; Online: Détection phénotypique d'une carbapénémase associée à une bêtalactamase à spectre élargi chez Klebsiella pneumoniae.

Chevet, K / Guyot, K / Mellon, G / Vidal, B / Couzigou, C / Misset, B / Janot, K / Lambert, T / Nguyen Van, J C

Medecine et maladies infectieuses

2012 Volume 42, Issue 1, Page(s) 33–35

Title translation	Phenotypic detection of carbapenemase associated with extended-spectrum beta-lactamase in Klebsiella pneumoniae.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/analysis ; Bacterial Proteins/genetics ; Bacterial Proteins/isolation & purification ; Drug Resistance, Multiple, Bacterial ; Genes, Bacterial ; Humans ; Klebsiella Infections/epidemiology ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/enzymology ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/isolation & purification ; Microbial Sensitivity Tests ; Paris/epidemiology ; Phenotype ; Substrate Specificity ; beta-Lactam Resistance ; beta-Lactamases/analysis ; beta-Lactamases/genetics ; beta-Lactams/pharmacology
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; beta-Lactams ; beta-Lactamases (EC 3.5.2.6)
Language	French
Publishing date	2012-01
Publishing country	France
Document type	Journal Article
ZDB-ID	423536-8
ISSN	1769-6690 ; 0399-077X ; 1166-8237
ISSN (online)	1769-6690
ISSN	0399-077X ; 1166-8237
DOI	10.1016/j.medmal.2011.11.002
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Article ; Online: Diagnosis and treatment strategies for community-acquired Streptococcus salivarius meningitis.

Vignier, N / Couzigou, C / Nguyen Van, J C / Gerber, S / Gaillard, S / Bruel, C / Misset, B / Kitzis, M D / Le Monnier, A

Medecine et maladies infectieuses

2014 Volume 44, Issue 1, Page(s) 42–44

MeSH term(s)	Anti-Bacterial Agents/therapeutic use ; Ceftriaxone/therapeutic use ; Cerebrospinal Fluid/microbiology ; Cerebrospinal Fluid Rhinorrhea/surgery ; Cerebrospinal Fluid Shunts ; Community-Acquired Infections/cerebrospinal fluid ; Community-Acquired Infections/diagnosis ; Community-Acquired Infections/drug therapy ; Community-Acquired Infections/microbiology ; DNA, Bacterial/cerebrospinal fluid ; Dexamethasone/therapeutic use ; Drug Therapy, Combination ; Female ; Fistula/complications ; Fistula/surgery ; Humans ; Magnetic Resonance Imaging ; Meningitis/cerebrospinal fluid ; Meningitis/diagnosis ; Meningitis/drug therapy ; Meningitis/microbiology ; Middle Aged ; Respiratory Tract Fistula/complications ; Respiratory Tract Fistula/surgery ; Ribotyping ; Sphenoid Sinusitis/complications ; Sphenoid Sinusitis/diagnostic imaging ; Sphenoid Sinusitis/microbiology ; Sphenoid Sinusitis/surgery ; Streptococcal Infections/cerebrospinal fluid ; Streptococcal Infections/diagnosis ; Streptococcal Infections/drug therapy ; Streptococcal Infections/microbiology ; Streptococcus/isolation & purification ; Subarachnoid Space ; Tomography, X-Ray Computed
Chemical Substances	Anti-Bacterial Agents ; DNA, Bacterial ; Ceftriaxone (75J73V1629) ; Dexamethasone (7S5I7G3JQL)
Language	English
Publishing date	2014-01
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	423536-8
ISSN	1769-6690 ; 0399-077X ; 1166-8237
ISSN (online)	1769-6690
ISSN	0399-077X ; 1166-8237
DOI	10.1016/j.medmal.2013.10.005
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Article: Résistance aux antibiotiques par diminution de la perméabilité chez les bactéries à gram négatif.

Nguyen Van, J C / Gutmann, L

Presse medicale (Paris, France : 1983)

1994 Volume 23, Issue 11, Page(s) 522, 527–31

Abstract: Due to their outer membrane, Gram negative bacteria are the only germs which can resist antibiotics by a mechanism of reduced permeability. This outer hydrophobic membrane allows hydrophilic molecules to pass only through its aqueous pores. The ... ...

Title translation	Resistance to antibiotics caused by decrease of the permeability in gram-negative bacteria.
Abstract	Due to their outer membrane, Gram negative bacteria are the only germs which can resist antibiotics by a mechanism of reduced permeability. This outer hydrophobic membrane allows hydrophilic molecules to pass only through its aqueous pores. The transmembrane pores have a trimere structure with a monomere component acting as an aqueous channel. Mean pore diameter is 1 to 1.2 nm. Changes in the absolute number of pores or in qualitative function reduce the diffusion of antibiotics entering the cell. This mechanism of reduced permeability can lead to cross resistance to several families of antibiotics. It is difficult to determine the clinical incidence since such resistances are not always detected. The species most often involved are enterobacteria including Klebsiella, Enterobacter, Serratia and Salmonella. For Pseudomonas aeruginosa, resistance to imipenem by reduced permeability results from a deficit in protein D2 and concerns 12 to 15% of the strains identified in French Hospitals. Reduced permeability is particularly effective when associated with another mechanism of resistance allowing the bacteria to express a higher level of resistance.
MeSH term(s)	4-Quinolones ; Anti-Bacterial Agents/pharmacokinetics ; Anti-Infective Agents/pharmacokinetics ; Cell Membrane/drug effects ; Cell Membrane/physiology ; Cell Membrane Permeability/drug effects ; Drug Resistance, Microbial ; Gram-Negative Bacteria/drug effects ; Gram-Negative Bacteria/ultrastructure ; In Vitro Techniques ; Lactams ; Porins/pharmacology
Chemical Substances	4-Quinolones ; Anti-Bacterial Agents ; Anti-Infective Agents ; Lactams ; Porins
Language	French
Publishing date	1994-03-19
Publishing country	France
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	120943-7
ISSN	2213-0276 ; 0755-4982 ; 0032-7867 ; 0301-1518
ISSN (online)	2213-0276
ISSN	0755-4982 ; 0032-7867 ; 0301-1518
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