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  1. Article ; Online: Pseudo-NRBC in the Mindray BC-6800Plus analyzer: A clue for diagnostic anticipation of fungemia. Experimental and preliminary clinical reports.

    Zheng, Xiaohe / La Gioia, Antonio / Fiorini, Fabiana / Wang, Dong / Zhang, Shihong / Marsano, Miriam / Nicastro, Carmine / Fumi, Maurizio / Luo, Jinzhu

    International journal of laboratory hematology

    2024  

    Abstract: Introduction: Candidemia can be a significant cause of death in immunosuppressed or debilitated patients particularly. Abnormalities of the instrumental cytograms of some hematological analyzers, such as Mindray BC-6800Plus, can be related to ... ...

    Abstract Introduction: Candidemia can be a significant cause of death in immunosuppressed or debilitated patients particularly. Abnormalities of the instrumental cytograms of some hematological analyzers, such as Mindray BC-6800Plus, can be related to circulating Candida. We studied the possible diagnostic usefulness of this information.
    Methods: A fungal bloodstream infection has been simulated by adding aliquots of Candida albicans, Candida parapsilosis, and Candida glabrata to 75 leftovers and anonymized peripheral blood samples. Cytographic abnormalities like those of experimental samples were used to select patients with possible fungemia. The microscopic review of peripheral blood smears constituted the confirmatory method.
    Results: In all experimental samples, the various Candida types caused pseudo-NRBC and morphological abnormalities of WNB and DIFF cytograms. Circulating blastospores, free or engulfed by neutrophils, were the microscopic findings in the peripheral blood smears. In the clinical verification, 72 patients were recruited based on the presence of an evocative cluster in the WNB and DIFF cytograms. The microscopic review of 39 out of 72 samples was positive for NRBC. According to blood cultures, light microscopy revealed fungal forms of several Candida or non-Candida types in the remaining 33 samples. Nine of these cases were not yet known to suffer from bloodstream infection.
    Conclusions: Although further confirmatory clinical studies are required for these diagnostic abilities, the BC 6800Plus cytographic abnormalities related to fungemia have proven helpful in rapidly monitoring persistent fungemia in already diagnosed patients. In unknown or undiagnosed cases, they could be the trigger point for the subsequent diagnostic-therapeutic pathway.
    Language English
    Publishing date 2024-05-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.14303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1499: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: The Dysregulation of the Monocyte-Dendritic Cell Interplay Is Associated with In-Hospital Mortality in COVID-19 Pneumonia.

    Galati, Domenico / Mallardo, Domenico / Nicastro, Carmine / Zanotta, Serena / Capitelli, Ludovica / Lombardi, Carmen / Baino, Bianca / Cavalcanti, Ernesta / Sale, Silvia / Labonia, Francesco / Boenzi, Rita / Atripaldi, Luigi / Ascierto, Paolo Antonio / Bocchino, Marialuisa

    Journal of clinical medicine

    2024  Volume 13, Issue 9

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2024-04-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm13092481
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  3. Article ; Online: New Insights into the Identification of Metabolites and Cytokines Predictive of Outcome for Patients with Severe SARS-CoV-2 Infection Showed Similarity with Cancer.

    Costantini, Susan / Madonna, Gabriele / Di Gennaro, Elena / Capone, Francesca / Bagnara, Palmina / Capone, Mariaelena / Sale, Silvia / Nicastro, Carmine / Atripaldi, Lidia / Fiorentino, Giuseppe / Parrella, Roberto / Montesarchio, Vincenzo / Atripaldi, Luigi / Ascierto, Paolo A / Budillon, Alfredo

    International journal of molecular sciences

    2023  Volume 24, Issue 5

    Abstract: SARS-CoV-2 infection is characterized by several clinical manifestations, ranging from the absence of symptoms to severe forms that necessitate intensive care treatment. It is known that the patients with the highest rate of mortality develop increased ... ...

    Abstract SARS-CoV-2 infection is characterized by several clinical manifestations, ranging from the absence of symptoms to severe forms that necessitate intensive care treatment. It is known that the patients with the highest rate of mortality develop increased levels of proinflammatory cytokines, called the "cytokine storm", which is similar to inflammatory processes that occur in cancer. Additionally, SARS-CoV-2 infection induces modifications in host metabolism leading to metabolic reprogramming, which is closely linked to metabolic changes in cancer. A better understanding of the correlation between perturbed metabolism and inflammatory responses is necessary. We evaluated untargeted plasma metabolomics and cytokine profiling via
    MeSH term(s) Humans ; COVID-19 ; SARS-CoV-2 ; Cytokines ; Neoplasms ; Lactates
    Chemical Substances Cytokines ; Lactates
    Language English
    Publishing date 2023-03-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24054922
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  4. Article ; Online: Immune profiling of SARS-CoV-2 epitopes in asymptomatic and symptomatic pediatric and adult patients.

    Tornesello, Anna Lucia / Botti, Chiara / Micillo, Alberto / Labonia, Francesco / Arpino, Sergio / Isgrò, Maria Antonietta / Meola, Serena / Russo, Luigi / Cavalcanti, Ernesta / Sale, Silvia / Nicastro, Carmine / Atripaldi, Luigi / Starita, Noemy / Cerasuolo, Andrea / Reimer, Ulf / Holenya, Pavlo / Buonaguro, Luigi / Buonaguro, Franco M / Tornesello, Maria Lina

    Journal of translational medicine

    2023  Volume 21, Issue 1, Page(s) 123

    Abstract: Background: The infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has unpredictable manifestations of coronavirus disease (COVID-19) and variable clinical course with some patients being asymptomatic whereas others experiencing ...

    Abstract Background: The infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has unpredictable manifestations of coronavirus disease (COVID-19) and variable clinical course with some patients being asymptomatic whereas others experiencing severe respiratory distress, or even death. We aimed to evaluate the immunoglobulin G (IgG) response towards linear peptides on a peptide array containing sequences from SARS-CoV-2, Middle East respiratory syndrome-related coronavirus (MERS) and common-cold coronaviruses 229E, OC43, NL63 and HKU1 antigens, in order to identify immunological indicators of disease outcome in SARS-CoV-2 infected patients.
    Methods: We included in the study 79 subjects, comprising 19 pediatric and 30 adult SARS-CoV-2 infected patients with increasing disease severity, from mild to critical illness, and 30 uninfected subjects who were vaccinated with one dose of SARS-CoV-2 spike mRNA BNT162b2 vaccine. Serum samples were analyzed by a peptide microarray containing 5828 overlapping 15-mer synthetic peptides corresponding to the full SARS-CoV-2 proteome and selected linear epitopes of spike (S), envelope (E) and membrane (M) glycoproteins as well as nucleoprotein (N) of MERS, SARS and coronaviruses 229E, OC43, NL63 and HKU1 (isolates 1, 2 and 5).
    Results: All patients exhibited high IgG reactivity against the central region and C-terminus peptides of both SARS-CoV-2 N and S proteins. Setting the threshold value for serum reactivity above 25,000 units, 100% and 81% of patients with severe disease, 36% and 29% of subjects with mild symptoms, and 8% and 17% of children younger than 8-years reacted against N and S proteins, respectively. Overall, the total number of peptides in the SARS-CoV-2 proteome targeted by serum samples was much higher in children compared to adults. Notably, we revealed a differential antibody response to SARS-CoV-2 peptides of M protein between adults, mainly reacting against the C-terminus epitopes, and children, who were highly responsive to the N-terminus of M protein. In addition, IgG signals against NS7B, NS8 and ORF10 peptides were found elevated mainly among adults with mild (63%) symptoms. Antibodies towards S and N proteins of other coronaviruses (MERS, 229E, OC43, NL63 and HKU1) were detected in all groups without a significant correlation with SARS-CoV-2 antibody levels.
    Conclusions: Overall, our results showed that antibodies elicited by specific linear epitopes of SARS-CoV-2 proteome are age dependent and related to COVID-19 clinical severity. Cross-reaction of antibodies to epitopes of other human coronaviruses was evident in all patients with distinct profiles between children and adult patients. Several SARS-CoV-2 peptides identified in this study are of particular interest for the development of vaccines and diagnostic tests to predict the clinical outcome of SARS-CoV-2 infection.
    MeSH term(s) Adult ; Child ; Humans ; Antibodies, Viral ; BNT162 Vaccine ; Coronavirus 229E, Human ; COVID-19/immunology ; Epitopes ; Immunoglobulin G ; Middle East Respiratory Syndrome Coronavirus ; Proteome ; SARS-CoV-2
    Chemical Substances Antibodies, Viral ; BNT162 Vaccine ; Epitopes ; Immunoglobulin G ; Proteome
    Language English
    Publishing date 2023-02-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2118570-0
    ISSN 1479-5876 ; 1479-5876
    ISSN (online) 1479-5876
    ISSN 1479-5876
    DOI 10.1186/s12967-023-03963-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Serum levels of SARS-CoV-2 nucleocapsid antigen associate with inflammatory status and disease severity in COVID-19 patients.

    Perna, Francesco / Bruzzaniti, Sara / Piemonte, Erica / Maddaloni, Valeria / Atripaldi, Lidia / Sale, Silvia / Sanduzzi, Alessandro / Nicastro, Carmine / Pepe, Nicola / Bifulco, Maurizio / Matarese, Giuseppe / Galgani, Mario / Atripaldi, Luigi

    Clinical immunology (Orlando, Fla.)

    2021  Volume 226, Page(s) 108720

    MeSH term(s) COVID-19/blood ; COVID-19/immunology ; COVID-19/virology ; Humans ; Inflammation/immunology ; Nucleocapsid/immunology ; Phosphoproteins ; SARS-CoV-2/pathogenicity ; Severity of Illness Index
    Chemical Substances Phosphoproteins
    Language English
    Publishing date 2021-04-02
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 1459903-x
    ISSN 1521-7035 ; 1521-6616
    ISSN (online) 1521-7035
    ISSN 1521-6616
    DOI 10.1016/j.clim.2021.108720
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 880: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: COVID-19 vaccination in adults with congenital heart disease: Real-world data from an Italian tertiary centre.

    Fusco, Flavia / Scognamiglio, Giancarlo / Merola, Assunta / Roma, Anna Selvaggia / Nicastro, Carmine / Spatarella, Micaela / D'Abbraccio, Maurizio / Di Mauro, Gabriella / Atripaldi, Umberto / Atripaldi, Lidia / Correra, Anna / Palma, Michela / Barracano, Rosaria / Borrelli, Nunzia / Capuano, Annalisa / Sarubbi, Berardo

    International journal of cardiology. Congenital heart disease

    2021  Volume 6, Page(s) 100266

    Abstract: Background: real-world data on COVID-19 vaccine safety, immunogenicity and acceptance in adults with congenital heart disease (ACHD) are lacking.: Methods: ACHD patients who were offered COVID-19 vaccination from January to June 2021 were included. ... ...

    Abstract Background: real-world data on COVID-19 vaccine safety, immunogenicity and acceptance in adults with congenital heart disease (ACHD) are lacking.
    Methods: ACHD patients who were offered COVID-19 vaccination from January to June 2021 were included. Data on adverse events, on patients' attitude towards vaccination and antispike IgG titre were retrospectively collected. A group of healthy individuals with similar age and sex undergoing vaccination was included for comparison.
    Results: 208 patients followed in a single ACHD tertiary centre (33.3 [26-45] years, 54% male) received COVID-19 vaccine, 65% vaccinated at our institution: 199 (96%) received Pfizer-BioNTech BNT162b2 vaccine, 4 (2%) Moderna-1273 and 5 (2%) AstraZeneca-ChAdOx1. Median follow-up after vaccination was 79 [57-96] days. No major adverse event was reported and the incidence of minor events was not different between ACHD patients and the control group. One patient was diagnosed with acute pericarditis. There were two deaths unrelated to the vaccine during follow-up. Three (1.5%) vaccinated patients tested positive for COVID-19. Antispike IgG titre, available in 159 (76%) patients, was 1334 [600-3401] BAU/ml, not significantly different from the control group (p=0.2). One patient with Fontan failure was seronegative. Advanced physiological stage was associated with lower antibody response, independently from previous viral exposure (p<0.0001). Fourteen percent refused COVID-19 vaccination at our institution. However, 50% of vaccinated patients declared to have been influenced by the discussion with the ACHD cardiologist and 66% of those vaccinated in situ reported that undergoing COVID-19 vaccination at the ACHD centre made them feel safer.
    Conclusion: COVID-19 vaccines appear safe in ACHD with satisfactory immunogenicity. However, the most vulnerable patients showed lower antibody response. ACHD team may play a key role in vaccine acceptance.
    Language English
    Publishing date 2021-10-28
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2666-6685
    ISSN (online) 2666-6685
    DOI 10.1016/j.ijcchd.2021.100266
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