LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Back to previous search Search history
Advanced search

Your last searches

  1. AU="Nicole Matejka"
  2. AU="Nunes, Pedro M. Sales"
  3. AU="Sánchez-Montero, María Teresa"
  4. AU="Jiexin Zhang"
  5. AU=Mladinic M
  6. AU="Canfarotta, Michael W"
  7. AU="Merani, Shaheed"
  8. AU="Tarver, James E"
  9. AU=Wirestam Lina
  10. AU="Karen Martz"
  11. AU="Yadav, Kanhaiya L."
  12. AU="Girmay, Tigisty"
  13. AU="Hain, Sofia"
  14. AU="de Kler, R C F"
  15. AU="Veness, R."

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: Chromatin Organization after High-LET Irradiation Revealed by Super-Resolution STED Microscopy

    Benjamin Schwarz / Nicole Matejka / Sarah Rudigkeit / Matthias Sammer / Judith Reindl

    International Journal of Molecular Sciences, Vol 25, Iss 1, p

    2024  Volume 628

    Abstract: Ion-radiation-induced DNA double-strand breaks can lead to severe cellular damage ranging from mutations up to direct cell death. The interplay between the chromatin surrounding the damage and the proteins responsible for damage recognition and repair ... ...

    Abstract Ion-radiation-induced DNA double-strand breaks can lead to severe cellular damage ranging from mutations up to direct cell death. The interplay between the chromatin surrounding the damage and the proteins responsible for damage recognition and repair determines the efficiency and outcome of DNA repair. The chromatin is organized in three major functional compartments throughout the interphase: the chromatin territories, the interchromatin compartment, and the perichromatin lying in between. In this study, we perform correlation analysis using super-resolution STED images of chromatin; splicing factor SC35, as an interchromatin marker; and the DNA repair factors 53BP1, Rad51, and γH2AX in carbon-ion-irradiated human HeLa cells. Chromatin and interchromatin overlap only in protruding chromatin branches, which is the same for the correlation between chromatin and 53BP1. In contrast, between interchromatin and 53BP1, a gap of (270 ± 40) nm is visible. Rad51 shows overlap with decondensed euchromatic regions located at the borders of condensed heterochromatin with further correlation with γH2AX. We conclude that the DNA damage is repaired in decondensed DNA loops in the perichromatin, located in the periphery of the DNA-dense chromatin compartments containing the heterochromatin. Proteins like γH2AX and 53BP1 serve as supporters of the chromatin structure.
    Keywords chromatin organization ; DNA repair ; super-resolution microscopy ; interchromatin ; perichromatin ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 571
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Perspectives of cellular communication through tunneling nanotubes in cancer cells and the connection to radiation effects

    Nicole Matejka / Judith Reindl

    Radiation Oncology, Vol 14, Iss 1, Pp 1-

    2019  Volume 11

    Abstract: Abstract Direct cell-to-cell communication is crucial for the survival of cells in stressful situations such as during or after radiation exposure. This communication can lead to non-targeted effects, where non-treated or non-infected cells show effects ... ...

    Abstract Abstract Direct cell-to-cell communication is crucial for the survival of cells in stressful situations such as during or after radiation exposure. This communication can lead to non-targeted effects, where non-treated or non-infected cells show effects induced by signal transduction from non-healthy cells or vice versa. In the last 15 years, tunneling nanotubes (TNTs) were identified as membrane connections between cells which facilitate the transfer of several cargoes and signals. TNTs were identified in various cell types and serve as promoter of treatment resistance e.g. in chemotherapy treatment of cancer. Here, we discuss our current understanding of how to differentiate tunneling nanotubes from other direct cellular connections and their role in the stress reaction of cellular networks. We also provide a perspective on how the capability of cells to form such networks is related to the ability to surpass stress and how this can be used to study radioresistance of cancer cells.
    Keywords Cellular communication ; Tunneling nanotubes ; Radioresistance ; Cancer ; Medical physics. Medical radiology. Nuclear medicine ; R895-920 ; Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282
    Subject code 610
    Language English
    Publishing date 2019-12-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top