Article ; Online: Acute and chronic ethanol administration: A potential model of behavioral sensitization using wheel-running in male CD1 mice.
Pharmacology, biochemistry, and behavior
2022 Volume 213, Page(s) 173336
Abstract: Rationale: We define behavioral sensitization as an augmented response to subsequent dosing after chronic intermittent administration of a drug. However, the biphasic effects of ethanol (EtOH), first stimulatory followed by depressive, make animal ... ...
Abstract | Rationale: We define behavioral sensitization as an augmented response to subsequent dosing after chronic intermittent administration of a drug. However, the biphasic effects of ethanol (EtOH), first stimulatory followed by depressive, make animal models of behavioral sensitization rare. Objectives: This study aimed to determine a dose of EtOH that did not depress wheel-running (WR) in CD1 mice and then to develop a model of EtOH-induced behavioral sensitization. Methods: For the first part of this study, male CD1 mice (n = 24, 6/group) were administered either phosphate buffer saline (PBS), 0.5 g/kg, 1 g/kg, or 2 g/kg EtOH at a volume of 3 ml/kg, intraperitoneally (IP). Mice were divided into equal groups and received the weight-based dose once daily on Days 1, 2, 3, 4, and 5. All mice received a challenge dose of 0.5 g/kg on Day 10. In both parts of the study, mice were habituated to the running wheel for 5 min prior to dosing and wheel running was measured for 10 min after each dose. Results: The acute dose-response of EtOH effects on wheel running determined a significant difference between doses in wheel running (p < 0.05), with a post-hoc analysis establishing that 0.5 g/kg EtOH resulted in significantly more WR compared to 2 g/kg EtOH (p < 0.05). The chronic study demonstrated a significant main effect of Day (1 vs. 5 vs. Challenge, p < 0.001) and an interaction between Day and Treatment, with post-hoc analysis determining the effect to be between PBS and EtOH WR on Day 5 (p < 0.05). In addition, Bonferroni post-hoc analysis determined no differences between Days in the PBS condition, but a significant difference in the EtOH condition between Day 1 and Day 5 (p < 0.001) and that difference from Day 1 persisted when comparing to the Challenge Day (p < 0.01). Conclusion: After chronic, intermittent, low dose administration of EtOH, male mice showed an increase in activity as measured by wheel running. Therefore, we laid the groundwork for a potentially useful rodent model for EtOH-induced behavioral sensitization. |
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MeSH term(s) | Alcoholism/metabolism ; Alcoholism/psychology ; Animals ; Behavior, Animal/drug effects ; Disease Models, Animal ; Drug Administration Schedule ; Ethanol/administration & dosage ; Ethanol/pharmacology ; Male ; Mice ; Motor Activity/drug effects ; Time Factors |
Chemical Substances | Ethanol (3K9958V90M) |
Language | English |
Publishing date | 2022-01-15 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 191042-5 |
ISSN | 1873-5177 ; 0091-3057 |
ISSN (online) | 1873-5177 |
ISSN | 0091-3057 |
DOI | 10.1016/j.pbb.2022.173336 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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