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Article ; Online: Recent contributions to mechanisms of Innate Immunity of Fish.

Nie, Pin

Developmental and comparative immunology

2023 Volume 151, Page(s) 105089

MeSH term(s)	Animals ; Immunity, Innate ; Fishes ; Fish Diseases
Language	English
Publishing date	2023-11-02
Publishing country	United States
Document type	Editorial
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2023.105089
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Article ; Online: Detection of Dermocystidium anguillae in imported elvers of American eel Anguilla rostrata in China.

Li, Dan / Nie, Pin / Liu, Yang

Folia parasitologica

2022 Volume 69

Abstract: In recent years, an emerging dermocystidiosis caused by Dermocystidium anguillae Spangenberg, 1975 has been found to pose a threat to the culture of American eel, Anguilla rostrata (Lesueur), as well as Chinese perch, Siniperca chuatsi (Basilewsky), in ... ...

Abstract	In recent years, an emerging dermocystidiosis caused by Dermocystidium anguillae Spangenberg, 1975 has been found to pose a threat to the culture of American eel, Anguilla rostrata (Lesueur), as well as Chinese perch, Siniperca chuatsi (Basilewsky), in China. Dermocystidium anguillae was originally described from European eel, Anguilla anguilla (Linnaeus), and it is thus important to identify the possible source of this pathogen. In the present study, we compared D. anguillae from European eels cultured in China with those from American eels. Molecular analysis showed that the SSU rDNA of D. anguillae infecting European eels was identical to that of D. anguillae infecting American eels, suggesting their conspecificity. To investigate the source of D. anguillae causing dermocystidiosis in American eels cultured in China, a specific PCR assay for the detection of D. anguillae was developed with high sensitivity (10
MeSH term(s)	Anguilla ; Animals ; China/epidemiology ; Water
Chemical Substances	Water (059QF0KO0R)
Language	English
Publishing date	2022-06-21
Publishing country	Czech Republic
Document type	Journal Article
ZDB-ID	162048-4
ISSN	1803-6465 ; 0015-5683
ISSN (online)	1803-6465
ISSN	0015-5683
DOI	10.14411/fp.2022.013
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Article ; Online: Retroposition of the Long Transcript from Multiexon IFN-β Homologs in Ancestry Vertebrate Gave Rise to the Proximal Transcription Elements of Intronless IFN-β Promoter in Humans.

Chen, Shan Nan / Gan, Zhen / Nie, Pin

Journal of immunology (Baltimore, Md. : 1950)

2021 Volume 207, Issue 10, Page(s) 2512–2520

Abstract: IFN-β is a unique member of type I IFN in humans and contains four positive regulatory domains (PRDs), I-II-III-IV, in its promoter, which are docking sites for transcription factors IFN regulatory factor (IRF) 3/7, NF-κB, IRF3/7, and activating ... ...

Abstract	IFN-β is a unique member of type I IFN in humans and contains four positive regulatory domains (PRDs), I-II-III-IV, in its promoter, which are docking sites for transcription factors IFN regulatory factor (IRF) 3/7, NF-κB, IRF3/7, and activating transcription factor 2/Jun proto-oncogene, respectively. In chicken IFN-β and zebrafish IFNφ1 promoters, a conserved PRD or PRD-like sequences have been reported. In this study, a type I IFN gene, named as xl-IFN1 in the amphibian model
MeSH term(s)	Animals ; Chickens ; Evolution, Molecular ; Humans ; Interferon-beta/genetics ; Introns/genetics ; Promoter Regions, Genetic/genetics ; Zebrafish
Chemical Substances	Interferon-beta (77238-31-4)
Language	English
Publishing date	2021-10-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.2100092
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Article ; Online: Molecular cloning and functional analyses of C-C motif chemokine ligand 3 (CCL3) in mandarin fish Siniperca chuatsi.

Liu, Xiao / Ji, Jia Xiang / Pang, An Ning / Li, Li / Nie, Pin / Zhang, Li Qiang / Zeng, Ke Wei / Chen, Shan Nan

Fish & shellfish immunology

2024 Volume 149, Page(s) 109614

Abstract: Chemokines are critical molecules involved in immune reaction and immune system homeostasis, and some chemokines play a role in antiviral immunity. It is not known if the C-C motif chemokine ligand 3 (CCL3), a member of the CC chemokine family, possesses ...

Abstract	Chemokines are critical molecules involved in immune reaction and immune system homeostasis, and some chemokines play a role in antiviral immunity. It is not known if the C-C motif chemokine ligand 3 (CCL3), a member of the CC chemokine family, possesses antiviral properties in fish. In this study, a ccl3 was cloned from the mandarin fish (Siniperca chuatsi), and it has an open reading frame (ORF) of 276 base pairs, which are predicted to encode a 91-amino acid peptide. Mandarin fish CCL3 revealed conserved sequence features with four cysteine residues and closely relationships with the CCL3s from other vertebrates based on the sequence alignment and phylogenetic analysis. The transcripts of ccl3 were notably enriched in immune-related organs, such as spleen and gills in healthy mandarin fish, and the ccl3 was induced in the isolated mandarin fish brain (MFB) cells following infection with infectious spleen and kidney necrosis virus (ISKNV). Moreover, in MFB cells, overexpression of CCL3 induced immune factors, such as IL1β, TNFα, MX, IRF1 and IFNh, and exhibited antiviral activity against ISKNV. This study sheds light on the immune role of CCL3 in immune response of mandarin fish, and its antiviral defense mechanism is of interest for further investigation.
Language	English
Publishing date	2024-05-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2024.109614
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Article ; Online: Identification of PGRP2 and its three splice variants in grass carp Ctenopharyngodon idella.

Jin, Yong / Li, Li / Li, Jun Hua / Nie, Pin

Fish & shellfish immunology

2022 Volume 127, Page(s) 933–938

Abstract: In this study, peptidoglycan recognition protein 2 (PGRP2) gene was cloned in grass carp Ctenopharyngodon idella, with the open reading frame (ORF) of PGRP2 being 1452 bp, encoding a protein of 483 amino acids. Three splice variants, PGRP2a, PGRP2b, and ... ...

Abstract	In this study, peptidoglycan recognition protein 2 (PGRP2) gene was cloned in grass carp Ctenopharyngodon idella, with the open reading frame (ORF) of PGRP2 being 1452 bp, encoding a protein of 483 amino acids. Three splice variants, PGRP2a, PGRP2b, and PGRP2c, were found also in grass carp with the absence of entire exon two and partial exon two of the PGRP2, and were predicted to have 124, 371 and 311 amino acids. But, they all have PGRP domain and signal peptide, except PGRP2a. The PGRP2 and its variants were expressed in all organs/tissues examined, and stimulated following PGN injection. It is further detected that the expression of gcPGRP2 and its variants was up-regulated after the single transfection of each of gcPGRP2 and its variant expression plasmids in CO cells. It is considered that the cloning of PGRP2 in grass carp provides a compositional completeness of PGRP members in this fish with the inclusion of previously reported PGRP5 and PGRP6.
MeSH term(s)	Amino Acid Sequence ; Amino Acids/genetics ; Animals ; Carps/genetics ; Carps/metabolism ; Cloning, Molecular ; Exons ; Fish Diseases/genetics ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Phylogeny
Chemical Substances	Amino Acids ; Fish Proteins
Language	English
Publishing date	2022-07-19
Publishing country	England
Document type	Journal Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2022.07.042
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Article ; Online: Liver X Receptor LXRα Promotes Grass Carp Reovirus Infection by Attenuating IRF3-CBP Interaction and Inhibiting RLR Antiviral Signaling.

Song, Yun Jie / Zhang, Jie / Xu, Zhen / Nie, Pin / Chang, Ming Xian

Journal of immunology (Baltimore, Md. : 1950)

2023 Volume 211, Issue 6, Page(s) 1006–1019

Abstract: Liver X receptors (LXRs) are nuclear receptors involved in metabolism and the immune response. Different from mammalian LXRs, which include two isoforms, LXRα and LXRβ, only a single LXRα gene exists in the piscine genomes. Although a study has suggested ...

Abstract	Liver X receptors (LXRs) are nuclear receptors involved in metabolism and the immune response. Different from mammalian LXRs, which include two isoforms, LXRα and LXRβ, only a single LXRα gene exists in the piscine genomes. Although a study has suggested that piscine LXR inhibits intracellular bacterial survival, the functions of piscine LXRα in viral infection are unknown. In this study, we show that overexpression of LXRα from grass carp (Ctenopharyngodon idellus), which is named as gcLXRα, increases host susceptibility to grass carp reovirus (GCRV) infection, whereas gcLXRα knockdown in CIK (C. idellus kidney) cells inhibits GCRV infection. Consistent with these functional studies, gcLXRα knockdown promotes the transcription of antiviral genes involved in the RIG-I-like receptor (RLR) antiviral signaling pathway, including IFN regulatory factor (IRF3) and the type I IFN IFN1. Further results show that gcLXRα knockdown induces the expression of CREB-binding protein (CBP), a transcriptional coactivator. In the knockdown of CBP, the inhibitory effect of gcLXRα knockdown in limiting GCRV infection is completely abolished. gcLXRα also interacts with IRF3 and CBP, which impairs the formation of the IRF3/CBP transcription complex. Moreover, gcLXRα heterodimerizes with RXRg, which cooperatively impair the transcription of the RLR antiviral signaling pathway and promote GCRV infection. Taken together, to our knowledge, our findings provide new insight into the functional correlation between nuclear receptor LXRα and the RLR antiviral signaling pathway, and they demonstrate that gcLXRα can impair the RLR antiviral signaling pathway and the production of type I IFN via forming gcLXRα/RXRg complexes and attenuating IRF3/CBP complexes.
MeSH term(s)	Animals ; Humans ; Antiviral Agents/pharmacology ; Reoviridae ; Liver X Receptors/metabolism ; Carps/metabolism ; CREB-Binding Protein/metabolism ; Reoviridae Infections ; Signal Transduction ; Interferon Type I/metabolism ; Fish Diseases ; Fish Proteins/genetics ; Mammals/metabolism ; Interferon Regulatory Factor-3/metabolism
Chemical Substances	Antiviral Agents ; Liver X Receptors ; CREB-Binding Protein (EC 2.3.1.48) ; Interferon Type I ; Fish Proteins ; IRF3 protein, human ; Interferon Regulatory Factor-3
Language	English
Publishing date	2023-08-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.2300214
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Article ; Online: Review of " Fish Defenses

Nie Pin

Parasites & Vectors, Vol 3, Iss 1, p

Volume 2. Pathogens, Parasites and Predators " by G. Zaccone, C. Perrière, A. Mathis, B. G. Kapoor (eds.)

2010 Volume 24

Abstract: Abstract Book review of " Fish Defenses: Volume 2. Pathogens, Parasites and Predators " by G. Zaccone, C. Perrière, A. Mathis, B. G. Kapoor (eds.) ...

Abstract	Abstract Book review of " Fish Defenses: Volume 2. Pathogens, Parasites and Predators " by G. Zaccone, C. Perrière, A. Mathis, B. G. Kapoor (eds.)
Keywords	Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Infectious and parasitic diseases ; RC109-216 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Language	English
Publishing date	2010-03-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nuclear import of IRF11 via the importin α/β pathway is essential for its antiviral activity

Li, Wenxing / Zhao, Gejie / Jiao, Zhiyuan / Xiang, Chao / Liang, Ying / Huang, Wenshu / Nie, Pin / Huang, Bei

Developmental and Comparative Immunology. 2023 Apr., v. 141 p.104649-

2023

Abstract: Interferon regulatory factor 11 (IRF11), an intriguing IRF member found only in fish species, has recently been shown to have antiviral properties that are dependent on its nuclear entry and DNA binding affinity. However, the mechanisms by which IRF11 ... ...

Abstract	Interferon regulatory factor 11 (IRF11), an intriguing IRF member found only in fish species, has recently been shown to have antiviral properties that are dependent on its nuclear entry and DNA binding affinity. However, the mechanisms by which IRF11 enters the nucleus are unknown. In the present study, we found orthologs of IRF11 in lamprey and lancelet species by combining positional, phylogenetic and structural comparison data, showing that this gene has an ancient origin. The IRF11 gene (AjIRF11) from the Japanese eel, Anguilla japonica, was subsequently characterized, and it was found that AjIRF11 has antiviral activities against spring viremia of carp virus (SVCV), which are accomplished by regulating the production of type I IFN and IFN-stimulated genes. In addition to its known DNA binding residues in the α3 helix, two residues in Loop 1, His40 and Trp46, are also involved in DNA binding and activation of the IFN promoter. Using immunofluorescence microscopy and site-directed mutagenesis analysis, we confirmed that full nuclear localization of AjIRF11 requires the bipartite nuclear localization sequence (NLS) spanning residues 75 to 101, as well as the monopartite NLS situated between residues 119 and 122. Coimmunoprecipitation assays confirmed that AjIRF11 interacts with importin α via its NLSs and can also bind to importin β directly, implying that IRF11 can be imported to the nucleus by one or more transport receptors.
Keywords	Anguilla japonica ; Carp sprivivirus ; DNA ; Petromyzontiformes ; antiviral properties ; fish ; fluorescence microscopy ; genes ; importins ; interferon regulatory factors ; nuclear localization signals ; phylogeny ; physiological transport ; precipitin tests ; site-directed mutagenesis ; IRF11 ; DNA binding ; Nuclear import ; Importin α/β ; Japanese eel
Language	English
Dates of publication	2023-04
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2023.104649
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Article: Morphological, histopathological and molecular characterization of parasitic ciliate Ichthyophthirius multifiliis infecting an indigenous and endangered fish, Schizothorax macropogon (Cyprinidae: Schizothoracinae) in high plateau, Tibet, China

Li, Wen Jing / Lyu, Xiao Meng / Nie, Pin / Liu, Yang

Aquaculture reports. 2022 May 24,

2022

Abstract: Ichthyophthirius multifiliis Fouquet, 1876 is an important parasitic pathogen of freshwater fish, with low host specificity and broad geographical distribution. However, the epizootic occurrence of ichthyophthiriasis in high plateau has been rarely ... ...

Abstract	Ichthyophthirius multifiliis Fouquet, 1876 is an important parasitic pathogen of freshwater fish, with low host specificity and broad geographical distribution. However, the epizootic occurrence of ichthyophthiriasis in high plateau has been rarely reported. In the present study, infection of I. multifiliis was found in juvenile fish of an indigenous and endangered fish Schizothorax macropogon Regan, 1905 (Cyprinidae: Schizothoracinae) in high plateau of Tibet, China, which caused mass mortality of the host. To understand the characteristics of this I. multifiliis isolate in Tibet, a morphological, histopathological and molecular study was performed to compare with another isolate of I. multifiliis from Tachysurus fulvidraco Richardson, 1846 in Guangdong province, China. Despite the distribution in different altitudes, obvious difference was not found in the two isolates in relation with infection site, morphology, ultrastructure and histopathology. The analysis of COX-1 gene, SSU rDNA and ITS1-5.8S rDNA-ITS2 sequences cloned from the two isolates in the present study showed that variable sites were found only in COX-1 gene sequences, which indicated that COX-1 gene was the most effective marker to distinguish different isolates of I. multifiliis. Phylogenetically, all I. multifiliis isolates can be divided into three genetically distinct groups, and the two isolates identified in the present study were placed in different groups. In consideration of the high plateau and low water temperature in Tibet, the possible differentiation of I. multifiliis is of interest for further investigation.
Keywords	Ichthyophthirius multifiliis ; Schizothorax ; Tachysurus fulvidraco ; aquaculture ; endangered species ; epizootic diseases ; freshwater fish ; genes ; geographical distribution ; histopathology ; host specificity ; juveniles ; mortality ; pathogens ; phylogeny ; ultrastructure ; water temperature ; China
Language	English
Dates of publication	2022-0524
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	2835391-2
ISSN	2352-5134
ISSN	2352-5134
DOI	10.1016/j.aqrep.2022.101192
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Article: Molecular and functional characterization of zinc ﬁnger aspartate-histidine-histidine-cysteine (DHHC)-type containing 1, ZDHHC1 in Chinese perch Siniperca chuatsi

Chen, Shan Nan / Zhang, Shan / Li, Li / Laghari, Zubair Ahmed / Nie, Pin

Fish & shellfish immunology. 2022 Nov., v. 130

2022

Abstract: In the present study, the zinc ﬁnger aspartate-histidine-histidine-cysteine (DHHC)-type containing 1 (ZDHHC1) gene was identified in a commercial fish, the Chinese perch Siniperca chuatsi. The ZDHHC1 has five putative transmembrane motifs and conserved ... ...

Abstract	In the present study, the zinc ﬁnger aspartate-histidine-histidine-cysteine (DHHC)-type containing 1 (ZDHHC1) gene was identified in a commercial fish, the Chinese perch Siniperca chuatsi. The ZDHHC1 has five putative transmembrane motifs and conserved DHHC domain, showing high amino-acid identity with other teleost fish, and vertebrate ZDHHC1 loci are conserved from fish to human. In vivo expression analysis indicated that ZDHHC1 gene was constitutively transcribed in all the examined organs/tissues, and was induced following infectious spleen and kidney necrosis virus (ISKNV) infection. It is further observed that ZDHHC1 interacts with MITA and the overexpression of ZDHHC1 in cells resulted in the upregulated expression of ISGs, such as Mx, RSAD2, IRF3 and type I IFNs such as IFNh and IFNc, exhibiting its antiviral function in fish as reported in mammals.
Keywords	Infectious spleen and kidney necrosis virus ; Siniperca chuatsi ; amino acids ; fish ; genes ; humans ; immunology ; shellfish ; zinc
Language	English
Dates of publication	2022-11
Size	p. 215-222.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2022.09.023
Database	NAL-Catalogue (AGRICOLA)

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