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  1. Article ; Online: Multi-Domain Feature Alignment for Face Anti-Spoofing.

    Zhang, Shizhe / Nie, Wenhui

    Sensors (Basel, Switzerland)

    2023  Volume 23, Issue 8

    Abstract: Face anti-spoofing is critical for enhancing the robustness of face recognition systems against presentation attacks. Existing methods predominantly rely on binary classification tasks. Recently, methods based on domain generalization have yielded ... ...

    Abstract Face anti-spoofing is critical for enhancing the robustness of face recognition systems against presentation attacks. Existing methods predominantly rely on binary classification tasks. Recently, methods based on domain generalization have yielded promising results. However, due to distribution discrepancies between various domains, the differences in the feature space related to the domain considerably hinder the generalization of features from unfamiliar domains. In this work, we propose a multi-domain feature alignment framework (MADG) that addresses poor generalization when multiple source domains are distributed in the scattered feature space. Specifically, an adversarial learning process is designed to narrow the differences between domains, achieving the effect of aligning the features of multiple sources, thus resulting in multi-domain alignment. Moreover, to further improve the effectiveness of our proposed framework, we incorporate multi-directional triplet loss to achieve a higher degree of separation in the feature space between fake and real faces. To evaluate the performance of our method, we conducted extensive experiments on several public datasets. The results demonstrate that our proposed approach outperforms current state-of-the-art methods, thereby validating its effectiveness in face anti-spoofing.
    Language English
    Publishing date 2023-04-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s23084077
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: DPPA5A suppresses the mutagenic TLS and MMEJ pathways by modulating the cryptic splicing of

    Jiang, Fangjie / Wang, Lin / Dong, Yuping / Nie, Wenhui / Zhou, Hu / Gao, Jing / Zheng, Ping

    Proceedings of the National Academy of Sciences of the United States of America

    2023  Volume 120, Issue 30, Page(s) e2305187120

    Abstract: Genetic alterations are often acquired during prolonged propagation of pluripotent stem cells (PSCs). This ruins the stem cell quality and hampers their full applications. Understanding how PSCs maintain genomic integrity would provide the clues to ... ...

    Abstract Genetic alterations are often acquired during prolonged propagation of pluripotent stem cells (PSCs). This ruins the stem cell quality and hampers their full applications. Understanding how PSCs maintain genomic integrity would provide the clues to overcome the hurdle. It has been known that embryonic stem cells (ESCs) utilize high-fidelity pathways to ensure genomic stability, but the underlying mechanisms remain largely elusive. Here, we show that many DNA damage response and repair genes display differential alternative splicing in mouse ESCs compared to differentiated cells. Particularly,
    MeSH term(s) Animals ; Mice ; Mutagens ; Nucleotidyltransferases/genetics ; Nucleotidyltransferases/metabolism ; Mouse Embryonic Stem Cells/metabolism ; NIH 3T3 Cells ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; DNA ; DNA Damage
    Chemical Substances Mutagens ; Nucleotidyltransferases (EC 2.7.7.-) ; Nuclear Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 2023-07-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2305187120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The chromosomal homology between dusky langur (Trachypithecus obscurus Ried, 1837) and human (Homo sapiens) revealed by chromosome painting

    Thongnetr, Weera / Sangpakdee, Wiwat / Tanomtong, Alongklod / Nie, Wenhui / Raso, Sayam / Pinthong, Krit

    Nucleus. 2022 Aug., v. 65, no. 2

    2022  

    Abstract: The presnt study provides illustated characterization of the chromosomal rearrangement of the male dusky langur (Trachypithecus obscurus Ried, 1837; TOB) using Chromosome Painting technique. Ear tissues specimen collection was kindly provided by Songkla ... ...

    Abstract The presnt study provides illustated characterization of the chromosomal rearrangement of the male dusky langur (Trachypithecus obscurus Ried, 1837; TOB) using Chromosome Painting technique. Ear tissues specimen collection was kindly provided by Songkla Zoo, Songkla province, Thailand. The fibroblast cells were cultured and cryopreserved after that all 24 Human chromosome-specific probes (22 autosomes, X and Y) were hybridized in situ to the TOB mitotic cells and captured and analyzed by GENUS software. The results showed that the TOB chromosomes were highly rearranged compared to the 30 conserved regions of human chromosomes of different sizes. Majority, TOB chromosomes, such as TOB chromosomes 1, 2, 3, 4, 9, 11, 12, 13, 15, 17, 18, 20, 21, X and Y which were homologous to only one human chromosome, are conserved.. Some of those were constituted of regions being two TOB chromosomes that composed one of HSA homologous regions or one TOB chromosomes homologous to two human chromosomes. This is the first report of molecular cytogenetics on TOB using all 24 human chromosome-specific probes. The chromosome mappingis useful for a comparisonof Langur either in the same or other genus that had been reported to elucidate and clarify evolutionary relationship of primates shared by the same common ancestor.
    Keywords Trachypithecus ; ancestry ; autosomes ; chromosome aberrations ; computer software ; cryopreservation ; fibroblasts ; humans ; males ; mitosis ; zoos ; Thailand
    Language English
    Dates of publication 2022-08
    Size p. 233-237.
    Publishing place Springer India
    Document type Article
    ZDB-ID 2589081-5
    ISSN 0976-7975 ; 0029-568X
    ISSN (online) 0976-7975
    ISSN 0029-568X
    DOI 10.1007/s13237-021-00381-0
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Comparative chromosome maps between the stone curlew and three ciconiiform species (the grey heron, little egret and crested ibis).

    Wang, Jinhuan / Su, Weiting / Hu, Yi / Li, Shengbin / O'Brien, Patricia C M / Ferguson-Smith, Malcolm A / Yang, Fengtang / Nie, Wenhui

    BMC ecology and evolution

    2022  Volume 22, Issue 1, Page(s) 23

    Abstract: Background: Previous cytogenetic studies show that the karyotypes of species in Ciconiiformes vary considerably, from 2n = 52 to 78. Their karyotypes include different numbers of small to minute bi-armed chromosomes that have evolved probably by fusions ...

    Abstract Background: Previous cytogenetic studies show that the karyotypes of species in Ciconiiformes vary considerably, from 2n = 52 to 78. Their karyotypes include different numbers of small to minute bi-armed chromosomes that have evolved probably by fusions of two ancestral microchromosomes, besides macrochromosomes and dot-like microchromosomes. However, it is impossible to define the inter-species homologies of such small-sized bi-armed chromosomes based on chromosome morphology and banding characteristics. Although painting probes from the chicken (Gallus gallus, GGA) chromosomes 1-9 and Z have been widely used to investigate avian chromosome homologies, GGA microchromosome probes are rarely used in these studies because most GGA microchromosome probes generated by flow sorting often contain multiple GGA microchromosomes. In contrast, the stone curlew (Burhinus oedicnemus, BOE, Charadriiformes) has an atypical low diploid chromosome number (42) karyotype and only 4 pairs of dot-like microchromosomes; a set of chromosome-specific painting probes that cover all BOE chromosomes has been generated. To get a genome-wide view of evolutionary chromosomal rearrangements in different lineages of Ciconiiformes, we used BOE painting probes instead of GGA painting probes to analyze the karyotypes of three ciconiiform species belonging to two different families: the eastern grey heron (Ardea cinerea, ACI, 2n = 64, Ardeidae), the little egret (Egretta garzetta, EGA, 2n = 64, Ardeidae) and the crested ibis (Nipponia nippon, NNI, 2n = 68, Threskiornithidae).
    Results: BOE painting probes display the same hybridization pattern on chromosomes of ACI and EGA, while a different hybridization pattern is observed on chromosomes of NNI. BOE autosome probes detected 21 conserved homologous segments and 5 fusions on the sixteen pairs of recognizable chromosomes of ACI and EGA, while 16 conserved homologous segments and 4 fusions were found on the twelve pairs of recognizable chromosomes of NNI. Only a portion of smaller bi-armed chromosomes in the karyotypes of the ciconiiform species could have evolved from fusions of ancestral microchromosomes. In particular BOE 5, which is the result of a fusion between two segments homologous to GGA 7 and 8 respectively, was retained also as either a single chromosome in ACI (ACI 5) and EGA (EGA 5) or had fused with a part of the BOE 10 equivalent in NNI (NNI 5).
    Conclusion: Our painting results indicate that different chromosome rearrangements occur in different ciconiiform lineages. Some of the small-sized bi-armed chromosomes in ACI, EGA and NNI are derived from the fusions of two microchromosomes, indicating that microchromosome fusions play an important role in ciconiiform chromosome evolution. The fusion segment homologous to GGA 7 and 8 is a potential cytogenetic signature that unites Ardeidae and Threskiornithidae.
    MeSH term(s) Animals ; Charadriiformes/genetics ; Chickens/genetics ; Chromosome Painting/methods ; Evolution, Molecular ; Humans ; Karyotype
    Language English
    Publishing date 2022-03-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2730-7182
    ISSN (online) 2730-7182
    DOI 10.1186/s12862-022-01979-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: A new species of forest hedgehog (

    Shi, Zifan / Yao, Hongfeng / He, Kai / Bai, Weipeng / Zhou, Jiajun / Fan, Jingyi / Su, Weiting / Nie, Wenhui / Yang, Shuzhen / Onditi, Kenneth O / Jiang, Xuelong / Chen, Zhongzheng

    ZooKeys

    2023  Volume 1185, Page(s) 143–161

    Abstract: The hedgehog ... ...

    Abstract The hedgehog genus
    Language English
    Publishing date 2023-11-28
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1185.111615
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Maps of Constitutive-Heterochromatin Distribution for Four

    Beklemisheva, Violetta R / Lemskaya, Natalya A / Prokopov, Dmitry Yu / Perelman, Polina L / Romanenko, Svetlana A / Proskuryakova, Anastasia A / Serdyukova, Natalya A / Utkin, Yaroslav A / Nie, Wenhui / Ferguson-Smith, Malcolm A / Yang, Fentang / Graphodatsky, Alexander S

    Genes

    2023  Volume 14, Issue 2

    Abstract: Constitutive-heterochromatin placement in the genome affects chromosome structure by occupying centromeric areas and forming large blocks. To investigate the basis for heterochromatin variation in the genome, we chose a group of species with a conserved ... ...

    Abstract Constitutive-heterochromatin placement in the genome affects chromosome structure by occupying centromeric areas and forming large blocks. To investigate the basis for heterochromatin variation in the genome, we chose a group of species with a conserved euchromatin part: the genus
    MeSH term(s) Animals ; Mustelidae/genetics ; Heterochromatin ; In Situ Hybridization, Fluorescence ; Euchromatin ; Carnivora/genetics ; Chromosome Structures
    Chemical Substances Heterochromatin ; Euchromatin
    Language English
    Publishing date 2023-02-14
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14020489
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Discovery and identification of a novel canine coronavirus causing a diarrhea outbreak in Vulpes.

    Liu, Yuting / Deng, Yao / Niu, Sheng / Zhu, Na / Song, Jingdong / Zhang, Xiaoshuang / Su, Weiting / Nie, Wenhui / Lu, Roujian / Irwin, David M / Gao, George Fu / Wang, Wenling / Wang, Qihui / Tan, Wenjie / Zhang, Shuyi

    Science bulletin

    2023  Volume 68, Issue 21, Page(s) 2598–2606

    Abstract: Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the ... ...

    Abstract Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the transmission of genetically diverse coronaviruses from natural hosts to humans. Here, we report the identification and characterization of a novel canine coronavirus (VuCCoV), which caused an epidemic of acute diarrhea in Vulpes (foxes) in Shenyang, China. The epidemic started on November 8, 2019, and caused more than 39,600 deaths by January 1, 2022. Full-length viral genomic sequences were obtained from 15 foxes with diarrhea at the early stage of this outbreak. The VuCCoV genome shared more than 90% nucleotide identity with canine coronavirus (CCoV) for three of the four structural genes, with the S gene showing a larger amount of divergence. In addition, 67% (10/15) of the VuCCoV genomes contained an open reading frame (ORF3) gene, which was previously only detected in CCoV-I genomes. Notably, VuCCoV had only two to three amino acid differences at the partial RNA-dependent RNA polymerase (RdRp) level to bat CoV, suggesting a close genetic relationship. Therefore, these novel VuCCoV genomes represent a previously unsampled lineage of CCoVs. We also show that the VuCCoV spike protein binds to canine and fox aminopeptidase N (APN), which may allow this protein to serve as an entry receptor. In addition, cell lines were identified that are sensitive to VuCCoV using a pseudovirus system. These data highlight the importance of identifying the diversity and distribution of coronaviruses in domestic animals, which could mitigate future outbreaks that could threaten livestock, public health, and economic growth.
    MeSH term(s) Animals ; Dogs ; Humans ; Foxes ; Coronavirus, Canine/genetics ; Animals, Wild ; SARS-CoV-2/genetics ; Animals, Domestic ; Disease Outbreaks/veterinary ; Diarrhea/epidemiology
    Language English
    Publishing date 2023-09-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2816140-3
    ISSN 2095-9281 ; 2095-9273
    ISSN (online) 2095-9281
    ISSN 2095-9273
    DOI 10.1016/j.scib.2023.09.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Maps of Constitutive-Heterochromatin Distribution for Four Martes Species (Mustelidae, Carnivora, Mammalia) Show the Formative Role of Macrosatellite Repeats in Interspecific Variation of Chromosome Structure

    Beklemisheva, Violetta R. / Lemskaya, Natalya A. / Prokopov, Dmitry Yu / Perelman, Polina L. / Romanenko, Svetlana A. / Proskuryakova, Anastasia A. / Serdyukova, Natalya A. / Utkin, Yaroslav A. / Nie, Wenhui / Ferguson-Smith, Malcolm A. / Yang, Fentang / Graphodatsky, Alexander

    Genes (Basel). 2023 Feb. 14, v. 14, no. 2

    2023  

    Abstract: Constitutive-heterochromatin placement in the genome affects chromosome structure by occupying centromeric areas and forming large blocks. To investigate the basis for heterochromatin variation in the genome, we chose a group of species with a conserved ... ...

    Abstract Constitutive-heterochromatin placement in the genome affects chromosome structure by occupying centromeric areas and forming large blocks. To investigate the basis for heterochromatin variation in the genome, we chose a group of species with a conserved euchromatin part: the genus Martes [stone marten (M. foina, 2n = 38), sable (M. zibellina, 2n = 38), pine marten (M. martes, 2n = 38), and yellow-throated marten (M. flavigula, 2n = 40)]. We mined the stone marten genome for the most abundant tandem repeats and selected the top 11 macrosatellite repetitive sequences. Fluorescent in situ hybridization revealed distributions of the tandemly repeated sequences (macrosatellites, telomeric repeats, and ribosomal DNA). We next characterized the AT/GC content of constitutive heterochromatin by CDAG (Chromomycin A3-DAPI-after G-banding). The euchromatin conservatism was shown by comparative chromosome painting with stone marten probes in newly built maps of the sable and pine marten. Thus, for the four Martes species, we mapped three different types of tandemly repeated sequences critical for chromosome structure. Most macrosatellites are shared by the four species with individual patterns of amplification. Some macrosatellites are specific to a species, autosomes, or the X chromosome. The variation of core macrosatellites and their prevalence in a genome are responsible for the species-specific variation of the heterochromatic blocks.
    Keywords Martes flavigula ; Martes foina ; Martes martes ; autosomes ; chromosome banding ; fluorescence ; genome ; heterochromatin ; hybridization ; interspecific variation ; ribosomal DNA ; telomeres
    Language English
    Dates of publication 2023-0214
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14020489
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Handelin extends lifespan and healthspan of Caenorhabditis elegans by reducing ROS generation and improving motor function.

    Zhang, Huijie / Qin, Jiawei / Lan, Xinqiang / Zeng, Weirong / Zhou, Jing / Huang, Tian-E / Xiao, Wan-Li / Wang, Qi-Quan / Sun, Sujiao / Su, Weiting / Nie, Wenhui / Yang, Shan / Yang, Ji / Gao, Qian / Xiang, Yang

    Biogerontology

    2022  Volume 23, Issue 1, Page(s) 115–128

    Abstract: Aging and aging-related disorders contribute to formidable socioeconomic and healthcare challenges. Several promising small molecules have been identified to target conserved genetic pathways delaying aging to extend lifespan and healthspan in many ... ...

    Abstract Aging and aging-related disorders contribute to formidable socioeconomic and healthcare challenges. Several promising small molecules have been identified to target conserved genetic pathways delaying aging to extend lifespan and healthspan in many organisms. We previously found that extract from an edible and medicinal plant Chrysanthemum indicum L. (C. indicum L.) protect skin from UVB-induced photoaging, partially by reducing reactive oxygen species (ROS) generation. Thus, we hypothesized that C. indicum L. and its biological active compound may extend lifespan and health span in vivo. We find that both water and ethanol extracts from C. indicum L. extended lifespan of Caenorhabditis elegans, with better biological effect on life extending for ethanol extracts. As one of the major biological active compounds, handelin extended lifespan of C. elegans too. RNA-seq analysis revealed overall gene expression change of C. elegans post stimulation of handelin focus on several antioxidative proteins. Handelin significantly reduced ROS level and maintained the number and morphology of mitochondria. Moreover, handelin improveed many C. elegans behaviors related to healthspan, including increased pharyngeal pumping and body movement. Muscle fiber imaging analyses revealed that handelin maintains muscle architecture by stabilizing myofilaments. In conclusion, our present study finds a novel compound handelin, from C. indicum L., which bring about biologically beneficial effects by mild stress response, termed as hormetin, that can extend both lifespan and healthspan in vivo on C. elegans. Further study on mammal animal model of natural aging or sarcopenia will verify the potential clinical value of handelin.
    MeSH term(s) Animals ; Caenorhabditis elegans/physiology ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Ethanol/pharmacology ; Longevity/physiology ; Mammals/metabolism ; Plant Extracts/pharmacology ; Reactive Oxygen Species/metabolism ; Terpenes
    Chemical Substances Caenorhabditis elegans Proteins ; Plant Extracts ; Reactive Oxygen Species ; Terpenes ; Ethanol (3K9958V90M) ; handelin (62687-22-3)
    Language English
    Publishing date 2022-01-17
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2047160-9
    ISSN 1573-6768 ; 1389-5729
    ISSN (online) 1573-6768
    ISSN 1389-5729
    DOI 10.1007/s10522-022-09950-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Species identification of crested gibbons (

    Nie, Wen-Hui / Wang, Jin-Huan / Su, Wei-Ting / Hu, Yu / He, Shui-Wang / Jiang, Xue-Long / He, Kai

    Zoological research

    2018  Volume 39, Issue 5, Page(s) 356–363

    Abstract: Gibbons and siamangs (Hylobatidae) are well-known for their rapid chromosomal evolution, which has resulted in high speciation rate within the family. On the other hand, distinct karyotypes do not prevent speciation, allowing interbreeding between ... ...

    Abstract Gibbons and siamangs (Hylobatidae) are well-known for their rapid chromosomal evolution, which has resulted in high speciation rate within the family. On the other hand, distinct karyotypes do not prevent speciation, allowing interbreeding between individuals in captivity, and the unwanted hybrids are ethically problematic as all gibbon species are endangered or critically endangered. Thus, accurate species identification is crucial for captive breeding, particularly in China where studbooks are unavailable. Identification based on external morphology is difficult, especially for hybrids, because species are usually similar in appearance. In this study, we employed G-banding karyotyping and fluorescence in situ hybridization (FISH) as well as a PCR-based approach to examine karyotypic characteristics and identify crested gibbons of the genus
    MeSH term(s) Animals ; Animals, Zoo ; Cell Nucleus/genetics ; China ; Endangered Species ; Genes/genetics ; Hylobates/classification ; Hylobates/genetics ; In Situ Hybridization, Fluorescence ; Karyotype ; Karyotyping ; Mitochondria/genetics ; Polymerase Chain Reaction
    Language English
    Publishing date 2018-04-03
    Publishing country China
    Document type Journal Article
    ISSN 2095-8137
    ISSN 2095-8137
    DOI 10.24272/j.issn.2095-8137.2018.036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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