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Article: Influence of cation content on adenosine triphosphate determinations in soil.

Eiland, F / Nielsen, B S

2013 Volume 5, Issue 2, Page(s) 129–137

Abstract: Adenosine triphosphate in soil is measured by the luciferin-luciferase bioluminescence method. Cation exchange in the ATP extraction procedure is used to reduce the content of, e.g., Fe, Al, and Cations in extracts. The efficiency of the cation exchange ... ...

Abstract	Adenosine triphosphate in soil is measured by the luciferin-luciferase bioluminescence method. Cation exchange in the ATP extraction procedure is used to reduce the content of, e.g., Fe, Al, and Cations in extracts. The efficiency of the cation exchange is determined by measuring the concentration of Fe by X-ray fluorescence spectrometry. The time dependence of the light emission from the bioluminescence process is investigated, and a method for correlating the ATP content and the light emission is proposed.
Language	English
Publishing date	2013-10-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	1462065-0
ISSN	1432-184X ; 0095-3628
ISSN (online)	1432-184X
ISSN	0095-3628
DOI	10.1007/BF02010504
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Article ; Online: Human sperm cells can form paracetamol metabolite AM404 that directly interferes with sperm calcium signalling and function through a CatSper-dependent mechanism.

Rehfeld, A / Frederiksen, H / Rasmussen, R H / David, A / Chaker, J / Nielsen, B S / Nielsen, J E / Juul, A / Skakkebæk, N E / Kristensen, D M

Human reproduction (Oxford, England)

2022 Volume 37, Issue 5, Page(s) 922–935

Abstract: Study question: Do paracetamol (N-acetyl-para-aminophenol (APAP) or acetaminophen) and/or its metabolites affect human sperm Ca2+-signalling and function?: Summary answer: While APAP itself does not interact with Ca2+-signalling in human sperm, its ... ...

Abstract	Study question: Do paracetamol (N-acetyl-para-aminophenol (APAP) or acetaminophen) and/or its metabolites affect human sperm Ca2+-signalling and function? Summary answer: While APAP itself does not interact with Ca2+-signalling in human sperm, its metabolite N-arachidonoyl phenolamine (AM404), produced via fatty acid amide hydrolase (FAAH), interferes with human sperm Ca2+-signalling and function through a suggested CatSper channel-dependent action. What is known already: Studies have shown that adult men with high urinary levels of over-the-counter mild analgesic APAP have impaired sperm motility and increased time-to-pregnancy. Study design, size, duration: This study consists of (i) an in vivo human pharmaceutical APAP exposure experiment to understand to what degree APAP reaches the sperm cells in the seminal fluid; (ii) in vitro calcium imaging and functional experiments in freshly donated human sperm cells to investigate CatSper channel-dependent activation by APAP and its metabolites; and (iii) experiments to understand the in situ capabilities of human sperm cells to form APAP metabolite AM404. Participants/materials, setting, methods: Three healthy young males participated in the in vivo human exposure experiment after prior consent. Human semen samples were provided by healthy young volunteer donors after prior consent on the day of the in vitro experiments. Main results and the role of chance: Pharmaceutical APAP exposure reaches the seminal plasma in high micromolar concentrations and accumulates in the seminal plasma between 3 and 5 days of exposure (P-value 0.023). APAP and its primary metabolite 4-aminophenol (4AP) do not interact with human sperm Ca2+-signalling. Instead, the APAP metabolite AM404 produced via FAAH interferes with human sperm Ca2+-signalling through a CatSper-dependent action. Also, AM404 significantly increases sperm cell penetration into viscous mucous (P-value of 0.003). FAAH is functionally expressed in human sperm cells in the neck/midpiece region, as evidenced by immunohistochemical staining and the ability of human sperm cells to hydrolyse the fluorogenic FAAH substrate arachidonyl 7-amino, 4-methyl coumarin amide in an FAAH-dependent manner. Importantly, human sperm cells have the capacity to form AM404 in situ after exposure to 4AP (P-value 0.0402 compared to vehicle-treated sperm cells). Limitations, reasons for caution: The experiments were conducted largely in vitro. Future studies are needed to test whether APAP can disrupt human sperm function in vivo through the action of AM404. Wider implications of the findings: We hypothesize that these observations could, at least in part, be responsible for the negative association between male urinary APAP concentrations, sperm motility and time-to-pregnancy. Study funding/competing interest(s): D.M.K. is funded by the Lundbeck Foundation, grant number R324-2019-1881, and the Svend Andersen Foundation. A.R. is funded by a BRIDGE-Translational Excellence Programme grant funded by the Novo Nordisk Foundation, grant agreement number: NNF18SA0034956. All authors declare no competing interests. Trial registration number: N/A.
MeSH term(s)	Acetaminophen/pharmacology ; Adult ; Arachidonic Acids ; Calcium/metabolism ; Calcium Channels/metabolism ; Humans ; Male ; Pharmaceutical Preparations/metabolism ; Progesterone/metabolism ; Sperm Motility ; Spermatozoa/metabolism
Chemical Substances	Arachidonic Acids ; Calcium Channels ; Pharmaceutical Preparations ; Acetaminophen (362O9ITL9D) ; Progesterone (4G7DS2Q64Y) ; Calcium (SY7Q814VUP) ; N-(4-hydroxyphenyl)arachidonylamide (XVJ94H0U21)
Language	English
Publishing date	2022-03-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632776-x
ISSN	1460-2350 ; 0268-1161 ; 1477-741X
ISSN (online)	1460-2350
ISSN	0268-1161 ; 1477-741X
DOI	10.1093/humrep/deac042
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Article: European & Danish biogas experience

Nielsen, B.S

Advances in pork production : proceedings of the ... Banff Pork Seminar. 2007, v. 18

2007

Keywords	biogas ; Denmark ; Europe
Language	English
Size	p. 237-243.
Document type	Article
Note	Meeting held January 16-19, 2007.
ISSN	1489-1395
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: DUF1220 protein domains drive proliferation in human neural stem cells and are associated with increased cortical volume in anthropoid primates.

Keeney, J G / Davis, J M / Siegenthaler, J / Post, M D / Nielsen, B S / Hopkins, W D / Sikela, J M

Brain structure & function

2014 Volume 220, Issue 5, Page(s) 3053–3060

Abstract: Genome sequences encoding DUF1220 protein domains show a burst in copy number among anthropoid species and especially humans, where they have undergone the greatest human lineage-specific copy number expansion of any protein coding sequence in the genome. ...

Abstract	Genome sequences encoding DUF1220 protein domains show a burst in copy number among anthropoid species and especially humans, where they have undergone the greatest human lineage-specific copy number expansion of any protein coding sequence in the genome. While DUF1220 copy number shows a dosage-related association with brain size in both normal populations and in 1q21.1-associated microcephaly and macrocephaly, a function for these domains has not yet been described. Here we provide multiple lines of evidence supporting the view that DUF1220 domains function as drivers of neural stem cell proliferation among anthropoid species including humans. First, we show that brain MRI data from 131 individuals across 7 anthropoid species shows a strong correlation between DUF1220 copy number and multiple brain size-related measures. Using in situ hybridization analyses of human fetal brain, we also show that DUF1220 domains are expressed in the ventricular zone and primarily during human cortical neurogenesis, and are therefore expressed at the right time and place to be affecting cortical brain development. Finally, we demonstrate that in vitro expression of DUF1220 sequences in neural stem cells strongly promotes proliferation. Taken together, these data provide the strongest evidence so far reported implicating DUF1220 dosage in anthropoid and human brain expansion through mechanisms involving increasing neural stem cell proliferation.
MeSH term(s)	Adult ; Animals ; Biological Evolution ; Brain/metabolism ; Brain/pathology ; Cell Proliferation/physiology ; Female ; Humans ; Male ; Neural Stem Cells/cytology ; Organ Size/physiology ; Primates ; Protein Structure, Tertiary/physiology ; Young Adult
Language	English
Publishing date	2014-06-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2273162-3
ISSN	1863-2661 ; 1863-2653
ISSN (online)	1863-2661
ISSN	1863-2653
DOI	10.1007/s00429-014-0814-9
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Article: The urokinase receptor associated protein (uPARAP/endo180): a novel internalization receptor connected to the plasminogen activation system.

Engelholm, L H / Nielsen, B S / Danø, K / Behrendt, N

Trends in cardiovascular medicine

2001 Volume 11, Issue 1, Page(s) 7–13

Abstract: The urokinase-mediated plasminogen activation system plays a central role in the extracellular proteolytic degradation reactions in cancer invasion. In this review article we discuss a number of recent findings identifying a new cellular receptor protein, ...

Abstract	The urokinase-mediated plasminogen activation system plays a central role in the extracellular proteolytic degradation reactions in cancer invasion. In this review article we discuss a number of recent findings identifying a new cellular receptor protein, uPARAP, that interacts with components of this proteolytic system. uPARAP is a high molecular weight type-1 membrane protein, belonging to the macrophage mannose receptor protein family. On the surface of certain cells, uPARAP forms a ternary complex with the pro-form of the urokinase-type plasminogen activator (uPA) and its primary receptor (uPAR). While the biological consequences of this reaction have not yet been verified experimentally, a likely event is ligand internalization because uPARAP is a constitutively recycling internalization receptor. uPARAP also binds at least one component, collagen type V, in the extracellular matrix meshwork, pointing to a potential role in proteolytic substrate presentation. Additional ligands have been proposed, including collagenase-3 and glycoproteins capable of interacting with one of the multiple carbohydrate recognition-type domains of uPARAP. In various adult tissues uPARAP is present on fibroblasts, macrophages and a subset of endothelial cells. In fetal tissues the protein has also been demonstrated in certain bone forming regions. Hypotheses on the physiological function of uPARAP include regulatory roles in extracellular proteolysis. This type of function would be likely to direct the local turnover of proteases and their substrate degradation products and thus may add to the complicated interplay between several cell types in governing restricted tissue degradation.
MeSH term(s)	Animals ; Humans ; Mannose-Binding Lectins ; Membrane Glycoproteins/analysis ; Membrane Glycoproteins/chemistry ; Membrane Glycoproteins/physiology ; Neoplasms/metabolism ; Peptide Hydrolases/metabolism ; Plasminogen Activators/chemistry ; Plasminogen Activators/physiology ; Protein Binding ; Receptors, Cell Surface/analysis ; Receptors, Cell Surface/chemistry ; Receptors, Cell Surface/physiology ; Receptors, Urokinase Plasminogen Activator ; Urokinase-Type Plasminogen Activator/chemistry ; Urokinase-Type Plasminogen Activator/physiology
Chemical Substances	MRC2 protein, human ; Mannose-Binding Lectins ; Membrane Glycoproteins ; PLAUR protein, human ; Receptors, Cell Surface ; Receptors, Urokinase Plasminogen Activator ; Peptide Hydrolases (EC 3.4.-) ; Plasminogen Activators (EC 3.4.21.-) ; Urokinase-Type Plasminogen Activator (EC 3.4.21.73)
Language	English
Publishing date	2001-06-08
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1097434-9
ISSN	1873-2615 ; 1050-1738
ISSN (online)	1873-2615
ISSN	1050-1738
DOI	10.1016/s1050-1738(01)00076-7
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Zs.A 3419: Show issues

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Article ; Online: Pleiotropic actions of miR-21 highlight the critical role of deregulated stromal microRNAs during colorectal cancer progression.

Bullock, M D / Pickard, K M / Nielsen, B S / Sayan, A E / Jenei, V / Mellone, M / Mitter, R / Primrose, J N / Thomas, G J / Packham, G K / Mirenzami, A H / Mirnezami, A H

Cell death & disease

2013 Volume 4, Page(s) e684

Abstract: The oncogene microRNA-21 (miRNA; miR-21) is overexpressed in most solid organ tumours; however, a recent examination of stage II colorectal cancer (CRC) specimens suggests this may be a stromal phenomenon and not only a feature of cancer cells. In vitro ... ...

Abstract	The oncogene microRNA-21 (miRNA; miR-21) is overexpressed in most solid organ tumours; however, a recent examination of stage II colorectal cancer (CRC) specimens suggests this may be a stromal phenomenon and not only a feature of cancer cells. In vitro and in vivo studies show that miR-21 has potent pro-metastatic effects in various malignant carcinoma cell lines. The tumour microenvironment has also been identified as a key actor during the metastatic cascade; however to date the significance of deregulated miR-21 expression within the cancer-associated stroma has not been examined. In the present study, a quantitative RT-PCR-based analysis of laser microdissected tissue confirmed that miR-21 expression is associated with a four-fold mean increase in CRC stroma compared with normal tissue. In situ hybridisation using locked nucleic acid probes localised miR-21 expression predominantly to fibroblasts within tumour-associated stroma. To study the molecular and biological impact of deregulated stromal miR-21 in CRC, stable ectopic expression was induced in immortalised fibroblasts. This resulted in upregulated α-smooth muscle actin expression implying miR-21 overexpression is driving the fibroblast-to-myofibroblast transdifferentiation. Conditioned medium from miR-21-overexpressing fibroblasts protected CRC cells from oxaliplatin-induced apoptosis and increased their proliferative capacity. 3D organotypic co-cultures containing fibroblasts and CRC cells revealed that ectopic stromal miR-21 expression was associated with increased epithelial invasiveness. Reversion-inducing cysteine-rich protein with kazal motifs, an inhibitor of matrix-remodelling enzyme MMP2, was significantly downregulated by ectopic miR-21 in established and primary colorectal fibroblasts with a reciprocal rise in MMP2 activity. Inhibition of MMP2 abrogated the invasion-promoting effects of ectopic miR-21. This data, which characterises a novel pro-metastatic mechanism mediated by miR-21 in the CRC stroma, highlights the importance of miRNA deregulation within the tumour microenvironment and identifies a potential application for stromal miRNAs as biomarkers in cancer.
MeSH term(s)	Adenocarcinoma/metabolism ; Adenocarcinoma/pathology ; Antineoplastic Agents/pharmacology ; Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; Cell Survival/drug effects ; Colorectal Neoplasms/metabolism ; Colorectal Neoplasms/pathology ; Drug Resistance, Neoplasm ; Fibroblasts/metabolism ; GPI-Linked Proteins/genetics ; GPI-Linked Proteins/metabolism ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Genetic Pleiotropy ; Humans ; Matrix Metalloproteinase 2/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Neoplasm Invasiveness ; Organoplatinum Compounds/pharmacology ; Oxaliplatin ; RNA Interference ; Stromal Cells/metabolism ; Tumor Microenvironment
Chemical Substances	Antineoplastic Agents ; GPI-Linked Proteins ; MIRN21 microRNA, human ; MicroRNAs ; Organoplatinum Compounds ; RECK protein, human ; Oxaliplatin (04ZR38536J) ; MMP2 protein, human (EC 3.4.24.24) ; Matrix Metalloproteinase 2 (EC 3.4.24.24)
Language	English
Publishing date	2013-06-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2541626-1
ISSN	2041-4889 ; 2041-4889
ISSN (online)	2041-4889
ISSN	2041-4889
DOI	10.1038/cddis.2013.213
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Article ; Online: The prognostic importance of miR-21 in stage II colon cancer: a population-based study.

Kjaer-Frifeldt, S / Hansen, T F / Nielsen, B S / Joergensen, S / Lindebjerg, J / Soerensen, F B / dePont Christensen, R / Jakobsen, A

British journal of cancer

2012 Volume 107, Issue 7, Page(s) 1169–1174

Abstract: Background: Despite several years of research and attempts to develop prognostic models a considerable fraction of stage II colon cancer patients will experience relapse within few years from their operation. The aim of the present study was to ... ...

Abstract	Background: Despite several years of research and attempts to develop prognostic models a considerable fraction of stage II colon cancer patients will experience relapse within few years from their operation. The aim of the present study was to investigate the prognostic importance of miRNA-21 (miR-21), quantified by in situ hybridisation, in a unique, large population-based cohort. Patients and methods: The study included 764 patients diagnosed with stage II colon cancer in Denmark in the year 2003. One section from a representative paraffin-embedded tumour tissue specimen from each patient was processed for analysis of miR-21 and quantitatively assessed by image analysis. Results: The miR-21 signal was predominantly observed in fibroblast-like cells located in the stromal compartment of the tumours. We found that patients expressing high levels of miR-21 had significantly inferior recurrence-free cancer-specific survival (RF-CSS): HR=1.26; 95% CI: 1.15-1.60; P<0.001. In Cox regression analysis, a high level of miR-21 retained its prognostic importance and was found to be significantly related to poor RF-CSS: HR=1.41; 95% CI: 1.19-1.67; P<0.001. Conclusion: The present study showed that increasing miR-21 expression levels were significantly correlated to decreasing RF-CSS. Further investigations of the clinical importance of miR-21 in the selection of high-risk stage II colon cancer patients are merited.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Cohort Studies ; Colonic Neoplasms/genetics ; Colonic Neoplasms/pathology ; Denmark ; Disease-Free Survival ; Female ; Humans ; Male ; MicroRNAs/genetics ; Middle Aged ; Neoplasm Recurrence, Local/genetics ; Neoplasm Recurrence, Local/pathology ; Neoplasm Staging ; Prognosis
Chemical Substances	MIRN21 microRNA, human ; MicroRNAs
Language	English
Publishing date	2012-07-21
Publishing country	England
Document type	Journal Article
ZDB-ID	80075-2
ISSN	1532-1827 ; 0007-0920
ISSN (online)	1532-1827
ISSN	0007-0920
DOI	10.1038/bjc.2012.365
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Article: Systemic administration of anti-urokinase plasminogen activator receptor monoclonal antibodies induces hepatic fibrin deposition in tissue-type plasminogen activator deficient mice.

Jögi, A / Pass, J / Høyer-Hansen, G / Lund, L R / Nielsen, B S / Danø, K / Rømer, J

Journal of thrombosis and haemostasis : JTH

2007 Volume 5, Issue 9, Page(s) 1936–1944

Abstract: Background: Degradation of extracellular matrix proteins, such as fibrin, is pivotal to tumor invasion. Inhibition of the interaction between urokinase plasminogen activator (u-PA) and its receptor (u-PAR), and hence pro-u-PA activation, is an ... ...

Abstract	Background: Degradation of extracellular matrix proteins, such as fibrin, is pivotal to tumor invasion. Inhibition of the interaction between urokinase plasminogen activator (u-PA) and its receptor (u-PAR), and hence pro-u-PA activation, is an attractive approach to anti-invasive cancer therapy. A number of inhibitors exist for the human system, but because of species specificity none of these are efficient in mice. We have recently generated an inhibitory monoclonal antibody (mAb) against mouse u-PAR (mR1) by immunization of u-PAR-deficient mice. Objectives: To evaluate the effect of mR1 in vivo in a physiological setting sensitive to deregulated fibrinolysis, we have administered mR1 systemically and quantitated the effect on liver fibrin accumulation. Methods: Wild-type and tissue-type plasminogen activator (t-PA) deficient mice were administered with mR1, or control antibody, during 6 weeks. Thereafter, the livers were retrieved and the amount of liver fibrin measured by unbiased morphometrical analysis of immunofluorescence signal. Results: Systemic administration of mR1 caused significantly increased fibrin signal in anti-u-PAR treated t-PA-deficient mice compared to mock-treated, which mimics the phenotype of u-PAR;t-PA double-deficient mice. Fibrin and fibronectin accumulated within the sinusoidal space and was infiltrated by inflammatory cells. Analysis of small and rare hepatic fibrin plaques observed in t-PA-deficient mice showed infiltrating macrophages that, contrary to surrounding Kuppfer cells, expressed u-PAR. Conclusion: We show that u-PAR-expressing macrophages are involved in cell-mediated fibrinolysis of liver fibrin deposits, and that the antimouse-u-PAR mAb is effective in vivo and thus suited for studies of the effect of targeting the u-PA/u-PAR interaction in mouse cancer models.
MeSH term(s)	Animals ; Antibodies, Monoclonal/administration & dosage ; Antibodies, Monoclonal/pharmacology ; Fibrin/metabolism ; Fluorescent Antibody Technique ; Liver/drug effects ; Liver/metabolism ; Mice ; Mice, Inbred C57BL ; Receptors, Cell Surface/immunology ; Receptors, Urokinase Plasminogen Activator ; Tissue Plasminogen Activator/genetics
Chemical Substances	Antibodies, Monoclonal ; PLAUR protein, human ; Plaur protein, mouse ; Receptors, Cell Surface ; Receptors, Urokinase Plasminogen Activator ; Fibrin (9001-31-4) ; Tissue Plasminogen Activator (EC 3.4.21.68)
Language	English
Publishing date	2007-08-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2112661-6
ISSN	1538-7836 ; 1538-7933
ISSN (online)	1538-7836
ISSN	1538-7933
DOI	10.1111/j.1538-7836.2007.02653.x
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Zs.A 5805: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Pharmacological properties of homomeric and heteromeric GluR1o and GluR3o receptors.

Nielsen, B S / Banke, T G / Schousboe, A / Pickering, D S

European journal of pharmacology

1998 Volume 360, Issue 2-3, Page(s) 227–238

Abstract: Homomeric and heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunits GluR1o and GluR3o were expressed in Spodoptera frugiperda (Sf9) insect cells. Membranes containing the recombinant receptors showed a doublet of bands ...

Abstract	Homomeric and heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunits GluR1o and GluR3o were expressed in Spodoptera frugiperda (Sf9) insect cells. Membranes containing the recombinant receptors showed a doublet of bands of the expected size (99-109 kDa) after western immunoblotting which was shifted to a single band upon deglycosylation. In (R,S)-[3H]AMPA binding experiments, high expression was seen (Bmax = 0.8-3.8 pmol/mg protein) along with high affinity binding to a single site (Kd, nM+/-S.D.): GluR1o, 32.5+/-2.7; GluR3o, 23.7+/-2.4; GluR1o + GluR3o, 18.1+/-2.9. The pharmacological profiles of these receptors resembled that of native rat brain AMPA receptors: AMPA analogues > L-glutamate > quinoxaline-2,3-diones > kainate. In the Xenopus oocyte expression system we had previously shown that the agonist (R,S)-2-amino-3-(3-carboxy-5-methyl-4-isoxazolyl)propionate (ACPA) exhibited an 11-fold selectivity for GluR3o vs. GluR1o. In this study, it was found that ACPA has 3-fold higher affinity at homomeric GluR3o and heteromeric receptors than at homomeric GluR1o, suggesting that its efficacy and/or desensitisation properties are different at GluR1o vs. GluR3o.
MeSH term(s)	Animals ; Baculoviridae ; Binding, Competitive ; Blotting, Western ; Glutamic Acid/pharmacology ; Glycosylation ; Mutagenesis ; Radioligand Assay ; Receptors, AMPA/agonists ; Receptors, AMPA/antagonists & inhibitors ; Receptors, AMPA/genetics ; Receptors, AMPA/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Spodoptera ; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/metabolism
Chemical Substances	Receptors, AMPA ; Recombinant Proteins ; glutamate receptor ionotropic, AMPA 3 ; Glutamic Acid (3KX376GY7L) ; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (77521-29-0) ; glutamate receptor ionotropic, AMPA 1 (TFZ3H25BS1)
Language	English
Publishing date	1998-11-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80121-5
ISSN	1879-0712 ; 0014-2999
ISSN (online)	1879-0712
ISSN	0014-2999
DOI	10.1016/s0014-2999(98)00668-2
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Article ; Online: Immunolymphoscintigraphy for metastatic sentinel nodes: test of a model.

Chakera, A H / Nielsen, B S / Madsen, J / Romer, J / Kristjansen, P E G / Buch, I / Binderup, T / Ingvar, C / Nalla, A / Kjaer, A / Hesse, B

International journal of molecular imaging

2011 Volume 2011, Page(s) 828151

Abstract: Aim. To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes. Methods. We selected one of four tumour-specific antibodies against human breast cancer and investigated (1), in immune- ... ...

Abstract	Aim. To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes. Methods. We selected one of four tumour-specific antibodies against human breast cancer and investigated (1), in immune-deficient (nude) mice with xenograft human breast cancer expressing the antigen if specific binding of the intratumorally injected, radioactively labelled, monoclonal antibody could be scintigraphically visualized, and (2) transportation to and retention in regional lymph nodes of the radioactively labelled antibody after subcutaneous injection in healthy rabbits. Results and Conclusion. Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.
Language	English
Publishing date	2011-04-28
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2595407-6
ISSN	2090-1720 ; 2090-1712
ISSN (online)	2090-1720
ISSN	2090-1712
DOI	10.1155/2011/828151
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