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Article: Chitosan nanoparticle and its effect on

Mohammadi, Naemeh / Safaie, Naser / Nikkhah, Maryam / Moradi, Sajad

2024 Volume 10, Issue 7, Page(s) e28666

Abstract: Fungal pathogen " ...

Abstract	Fungal pathogen "
Language	English
Publishing date	2024-03-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e28666
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Article ; Online: A FRET-based aptasensor for the detection of α-synuclein oligomers as biomarkers of Parkinson's disease.

Saedi, Zeinab / Nikkhah, Maryam

Analytical methods : advancing methods and applications

2022 Volume 14, Issue 30, Page(s) 2937–2944

Abstract: Soluble oligomers of α-synuclein (α-syn) are known to be responsible for neuronal death in the early stages of Parkinson's disease (PD). Thus, the development of a simple, rapid, and inexpensive method for the detection of α-syn oligomers can help PD ... ...

Abstract	Soluble oligomers of α-synuclein (α-syn) are known to be responsible for neuronal death in the early stages of Parkinson's disease (PD). Thus, the development of a simple, rapid, and inexpensive method for the detection of α-syn oligomers can help PD diagnosis before irreversible damage to the brain tissue occurs. The present study is aimed at developing a FRET-based aptasensor for the selective and sensitive detection of α-syn oligomers. Herein, FAM-labeled aptamer adsorption on graphene oxide (GO) resulted in fluorescence quenching of FAM. The binding of α-syn oligomers to the aptamer led to the recovery of the fluorescence intensity. Under optimized conditions, the developed biosensor showed two linear response ranges from 10-100 nM and 250 nM to 2 μM in α-syn oligomer detection. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 6.3 nM and 19.3 nM, respectively. Furthermore, the performance of the sensing platform in the detection of the target analyte in biological matrices was demonstrated by the assay of α-syn oligomers in spiked human saliva samples. According to the obtained results, this sensing platform has a good performance for α-syn oligomer detection and it can be considered as a promising candidate for the early diagnosis of PD.
MeSH term(s)	Biomarkers ; Brain/metabolism ; Fluorescence Resonance Energy Transfer ; Humans ; Parkinson Disease/diagnosis ; Parkinson Disease/metabolism ; alpha-Synuclein/metabolism
Chemical Substances	Biomarkers ; alpha-Synuclein
Language	English
Publishing date	2022-08-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d2ay00611a
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Article: A FRET-based aptasensor for the detection of α-synuclein oligomers as biomarkers of Parkinson's disease

Saedi, Zeinab / Nikkhah, Maryam

Analytical methods. 2022 Aug. 4, v. 14, no. 30

2022

Abstract	Soluble oligomers of α-synuclein (α-syn) are known to be responsible for neuronal death in the early stages of Parkinson's disease (PD). Thus, the development of a simple, rapid, and inexpensive method for the detection of α-syn oligomers can help PD diagnosis before irreversible damage to the brain tissue occurs. The present study is aimed at developing a FRET-based aptasensor for the selective and sensitive detection of α-syn oligomers. Herein, FAM-labeled aptamer adsorption on graphene oxide (GO) resulted in fluorescence quenching of FAM. The binding of α-syn oligomers to the aptamer led to the recovery of the fluorescence intensity. Under optimized conditions, the developed biosensor showed two linear response ranges from 10–100 nM and 250 nM to 2 μM in α-syn oligomer detection. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 6.3 nM and 19.3 nM, respectively. Furthermore, the performance of the sensing platform in the detection of the target analyte in biological matrices was demonstrated by the assay of α-syn oligomers in spiked human saliva samples. According to the obtained results, this sensing platform has a good performance for α-syn oligomer detection and it can be considered as a promising candidate for the early diagnosis of PD.
Keywords	Parkinson disease ; adsorption ; aptasensors ; biomarkers ; brain ; death ; detection limit ; early diagnosis ; fluorescence ; graphene oxide ; humans ; neurons ; oligonucleotides ; saliva
Language	English
Dates of publication	2022-0804
Size	p. 2937-2944.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d2ay00611a
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Article ; Online: DNA binding properties and cytotoxic effects of two double rollover cycloplatinated (II) complexes on cancer cell lines.

Shabaninejad, Zahra / Nikkhah, Maryam / Nabavizadeh, S Masoud

Journal of inorganic biochemistry

2023 Volume 243, Page(s) 112194

Abstract: In this study, the DNA binding capacity and cytotoxic effects of two double rollovers cycloplatinated complexes, [ ... ...

Abstract	In this study, the DNA binding capacity and cytotoxic effects of two double rollovers cycloplatinated complexes, [Pt
MeSH term(s)	Cisplatin/pharmacology ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/chemistry ; DNA/chemistry ; Cell Line, Tumor ; Spectrum Analysis ; Coordination Complexes/pharmacology ; Coordination Complexes/chemistry ; Neoplasms
Chemical Substances	Cisplatin (Q20Q21Q62J) ; Antineoplastic Agents ; DNA (9007-49-2) ; Coordination Complexes
Language	English
Publishing date	2023-03-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	162843-4
ISSN	1873-3344 ; 0162-0134
ISSN (online)	1873-3344
ISSN	0162-0134
DOI	10.1016/j.jinorgbio.2023.112194
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Article ; Online: Spectroscopic analysis of recombinant human growth hormone in the presence of sucrose and trehalose.

Kamrani, Solmaz / Yaghmaei, Parichehreh / Nikkhah, Maryam / Hosseinkhani, Saman

Biotechnology and applied biochemistry

2023 Volume 70, Issue 4, Page(s) 1543–1553

Abstract: Recombinant human growth hormone (rhGH) is a therapeutic protein, associated with various human diseases, such as growth hormone deficiency. One of the interesting issues in the formulation of therapeutic proteins is excipients like disaccharides. In the ...

Abstract	Recombinant human growth hormone (rhGH) is a therapeutic protein, associated with various human diseases, such as growth hormone deficiency. One of the interesting issues in the formulation of therapeutic proteins is excipients like disaccharides. In the current study, we try to compare the effect of sucrose and trehalose on the structure of rhGH in the liquid state at 25°C and 55°C. We use spectroscopic techniques including intrinsic and extrinsic fluorescence, Fourier-transform infrared (FTIR), circular dichroism (CD), dynamic light scattering (DLS), and time-resolved fluorescence. FTIR shows a slight change in the secondary structure of rhGH in presence of the sugars as sucrose is more effective than trehalose. Fluorescence investigations also confirm the enhancements of folding of rhGH and fluorescein isothiocyanate (FITC)-rhGH in presence of sucrose (1.5-fold more than trehalose). Also, we studied sucrose's effect on the rete of aggregation of rhGH using spectroscopy of Congo red, and fluorescence imaging of thioflavin T (ThT)-treated samples. It can be suggested that sucrose facilitates the amyloid formation of rhGH during 20 days of incubation at 37°C. This study will help to understand the growth hormone structural behavior in the liquid state in the presence of sucrose and trehalose in vitro.
MeSH term(s)	Humans ; Human Growth Hormone/chemistry ; Sucrose/chemistry ; Trehalose/chemistry ; Recombinant Proteins ; Growth Hormone/chemistry ; Spectrum Analysis
Chemical Substances	Human Growth Hormone (12629-01-5) ; Sucrose (57-50-1) ; Trehalose (B8WCK70T7I) ; Recombinant Proteins ; Growth Hormone (9002-72-6)
Language	English
Publishing date	2023-02-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	883433-7
ISSN	1470-8744 ; 0885-4513
ISSN (online)	1470-8744
ISSN	0885-4513
DOI	10.1002/bab.2452
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Article ; Online: A turn-off fluorimetric -aptasensor for early detection of apoptosis inside the cells.

Salmani-Zarchi, Hamed / Borghei, Yasaman-Sadat / Nikkhah, Maryam

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2023 Volume 300, Page(s) 122933

Abstract: To detect cytochrome c (Cyt c) as an important biomarker of apoptosis inside the cells, a simple, label-free, fluorometric detection method has been presented. For this purpose, an aptamer/gold nanocluster probe (Aptamer@AuNCs) was produced which could ... ...

Abstract	To detect cytochrome c (Cyt c) as an important biomarker of apoptosis inside the cells, a simple, label-free, fluorometric detection method has been presented. For this purpose, an aptamer/gold nanocluster probe (Aptamer@AuNCs) was produced which could specifically bind to Cyt c leading to fluorescence quenching of AuNCs. The developed aptasensor showed two linear ranges of 1-80 μM and 100-1000 μM and a detection limit of 0.77 μM and 297.5 μM, respectively. This platform was successfully used to assay Cyt c release inside the apoptotic cells and their cell lysate. Aptamer@AuNC due to its enzyme-like properties could replace antibodies in Cyt c detection by conventional blotting techniques.
MeSH term(s)	Metal Nanoparticles ; Fluorometry/methods ; Apoptosis ; Aptamers, Nucleotide ; Cytochromes c ; Gold ; Limit of Detection ; Biosensing Techniques/methods
Chemical Substances	Aptamers, Nucleotide ; Cytochromes c (9007-43-6) ; Gold (7440-57-5)
Language	English
Publishing date	2023-05-29
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2023.122933
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Article ; Online: A comparative study of structural and catalytic activity alterations in firefly luciferase induced by carbon quantum dots containing amine and carboxyl functional groups.

Rad-Faraji, Mehrnaz / Mousazadeh, Marziyeh / Nikkhah, Maryam / Rezaei, Aram / Moradi, Sajad / Hosseinkhani, Saman

International journal of biological macromolecules

2024 Volume 260, Issue Pt 2, Page(s) 129503

Abstract: Despite of growing interest in use of carbon-based nanomaterials as carriers of functional proteins, less attention has been paid to the effects of these nanomaterials on the structure and function of the proteins. In this study, with the aim of shedding ...

Abstract	Despite of growing interest in use of carbon-based nanomaterials as carriers of functional proteins, less attention has been paid to the effects of these nanomaterials on the structure and function of the proteins. In this study, with the aim of shedding light on the mechanisms of interaction between carbon-based nanomaterials and proteins, the interactions of carbon quantum dots (CQDs) containing amine (CQD-NH
MeSH term(s)	Quantum Dots/chemistry ; Luciferases, Firefly ; Amines ; Carbon/chemistry ; Luciferases/metabolism ; Amino Acids
Chemical Substances	Luciferases, Firefly (EC 1.13.12.7) ; Amines ; Carbon (7440-44-0) ; Luciferases (EC 1.13.12.-) ; Amino Acids
Language	English
Publishing date	2024-01-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.129503
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Article ; Online: A review of recent developments in the application of nanostructures for sperm cryopreservation.

Zandiyeh, Saeed / Kalantari, Hamid / Fakhri, Ali / Nikkhah, Maryam / Janani, Baadal Jushi / Sabbaghian, Marjan

Cryobiology

2024 Volume 115, Page(s) 104890

Abstract: In the 1970s, sperm cryopreservation was presented as a unique route to fertility preservation. The ability to cryopreserve sperm from all species is challenging. The sperm cryopreservation process encompasses various cellular stresses such as increased ... ...

Abstract	In the 1970s, sperm cryopreservation was presented as a unique route to fertility preservation. The ability to cryopreserve sperm from all species is challenging. The sperm cryopreservation process encompasses various cellular stresses such as increased osmotic pressure, ice crystal formation, and thermal shock, therefore decreasing the quality of sperm. The nanostructures due to their inherent features such as reactivity, high uptake, active surface area, and antioxidant activity, have contributed to modifying freezing protocols. In this review, the current state of the art with regards to emerging applications of nanotechnology in sperm cryopreservation are reviewed, some of the most promising advances are summarized, and the limitations and advantages are comprehensively discussed.
Language	English
Publishing date	2024-04-03
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	80098-3
ISSN	1090-2392 ; 0011-2240
ISSN (online)	1090-2392
ISSN	0011-2240
DOI	10.1016/j.cryobiol.2024.104890
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Article ; Online: Aptasensor for ovarian cancer biomarker detection using nanostructured gold electrodes.

Amirabadizadeh, Masood / Siampour, Hossein / Abbasian, Sara / Nikkhah, Maryam / Moshaii, Ahmad

Mikrochimica acta

2023 Volume 191, Issue 1, Page(s) 2

Abstract: The development of an electrochemical aptasensor for the detection of CA125 as an ovarian cancer biomarker using gold nanostructures (GNs) modified electrodes is reported. The GNs were deposited on the surface of fluorine-doped tin oxide electrodes using ...

Abstract	The development of an electrochemical aptasensor for the detection of CA125 as an ovarian cancer biomarker using gold nanostructures (GNs) modified electrodes is reported. The GNs were deposited on the surface of fluorine-doped tin oxide electrodes using a simple electrochemical method and the effects of pH and surfactant concentration on the topography and electrochemical properties of the resulting GNs modified electrodes were investigated. The electrodes were characterized using field-emission scanning electron microscopy and X-ray diffraction, cyclic voltammetry, and electrochemical impedance spectroscopy. The best electrode, in terms of the uniformity of the deposited GNs and the increase in electroactive surface area, was used for development of an aptasensor for CA125 tumor marker detection in human serum. Signal amplification was done by using aptamer-conjugated gold nanorods resulting in the detection limit of 2.6 U/ml and a linear range of 10 to 800 U/ml. The results showed that without the need for expensive antibodies, the developed aptasensor could specifically measure the clinically relevant concentrations of the tumor marker in human serum.
MeSH term(s)	Humans ; Biomarkers, Tumor ; Metal Nanoparticles/chemistry ; Gold/chemistry ; Aptamers, Nucleotide/chemistry ; Neoplasms ; Nanostructures ; Electrodes
Chemical Substances	Biomarkers, Tumor ; Gold (7440-57-5) ; Aptamers, Nucleotide
Language	English
Publishing date	2023-12-02
Publishing country	Austria
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	89-9
ISSN	1436-5073 ; 0026-3672
ISSN (online)	1436-5073
ISSN	0026-3672
DOI	10.1007/s00604-023-06072-8
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Article ; Online: Label-Free and Bioluminescence-Based Nano-Biosensor for ATP Detection.

Karimi, Elham / Nikkhah, Maryam / Hosseinkhani, Saman

Biosensors

2022 Volume 12, Issue 11

Abstract: A bioluminescence-based assay for ATP can measure cell viability. Higher ATP concentration indicates a higher number of living cells. Thus, it is necessary to design an ATP sensor that is low-cost and easy to use. Gold nanoparticles provide excellent ... ...

Abstract	A bioluminescence-based assay for ATP can measure cell viability. Higher ATP concentration indicates a higher number of living cells. Thus, it is necessary to design an ATP sensor that is low-cost and easy to use. Gold nanoparticles provide excellent biocompatibility for enzyme immobilization. We investigated the effect of luciferase proximity with citrate-coated gold, silver, and gold-silver core-shell nanoparticles, gold nanorods, and BSA-Au nanoclusters. The effect of metal nanoparticles on the activity of luciferases was recorded by the luminescence assay, which was 3-5 times higher than free enzyme. The results showed that the signal stability in presence of nanoparticles improved and was reliable up to 6 h for analytes measurements. It has been suggested that energy is mutually transferred from luciferase bioluminescence spectra to metal nanoparticle surface plasmons. In addition, we herein report the 27-base DNA aptamer for adenosine-5'-triphosphate (ATP) as a suitable probe for the ATP biosensor based on firefly luciferase activity and AuNPs. Due to ATP application in the firefly luciferase reaction, the increase in luciferase activity and improved detection limits may indicate more stability or accessibility of ATP in the presence of nanoparticles. The bioluminescence intensity increased with the ATP concentration up to 600 µM with a detection limit of 5 µM for ATP.
MeSH term(s)	Gold ; Metal Nanoparticles ; Silver ; Luciferases, Firefly/genetics ; Luciferases, Firefly/metabolism ; Adenosine Triphosphate ; Luciferases
Chemical Substances	Gold (7440-57-5) ; Silver (3M4G523W1G) ; Luciferases, Firefly (EC 1.13.12.7) ; Adenosine Triphosphate (8L70Q75FXE) ; Luciferases (EC 1.13.12.-)
Language	English
Publishing date	2022-10-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios12110918
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