LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: Polyethylene Glycol -Mediated Exosome Isolation: A Method for Exosomal RNA Analysis.

    Teflischi Gharavi, Abdulwahab / Niknejad, Azadeh / Irian, Saeed / Rahimi, Amirabbas / Salimi, Mona

    Iranian biomedical journal

    2024  

    Abstract: Background: ExoRNAs offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce ... ...

    Abstract Background: ExoRNAs offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce an appropriate strategy to extract RNA from cancer-derived exosomes for further RNA analysis.
    Methods: Exosomes were isolated through ultracentrifugation, precipitation kit, and size exclusion column chromatography, and then characterized by DLS and TEM, followed by extracting total RNA. The quality and quantity of the extracted RNAs were assessed by a NanoDrop and 2.5% agarose gel electrophoresis.
    Results: Extracted exosomes displayed a similar range of size and morphology. We found that PEG-precipitation method resulted in a higher RNA yield with a 260/280 ratio of 1.9. The obtained exoRNA appeared as a smear in the agarose gel, indicative of small exoRNAs.
    Conclusion: We provide researchers a suitable approach to isolate exosomes based on yield and purity of exoRNA.
    Language English
    Publishing date 2024-02-24
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2489282-8
    ISSN 2008-823X ; 1028-852X
    ISSN (online) 2008-823X
    ISSN 1028-852X
    DOI 10.61186/ibj.4129
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6776: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Harnessing exosomes as a platform for drug delivery in breast cancer: A systematic review for

    Gharavi, Abdulwahab Teflischi / Irian, Saeed / Niknejad, Azadeh / Parang, Keykavous / Salimi, Mona

    Molecular therapy. Oncology

    2024  Volume 32, Issue 2, Page(s) 200800

    Abstract: Breast cancer remains a significant global health concern, emphasizing the critical need for effective treatment strategies, especially targeted therapies. This systematic review summarizes the findings ... ...

    Abstract Breast cancer remains a significant global health concern, emphasizing the critical need for effective treatment strategies, especially targeted therapies. This systematic review summarizes the findings from
    Language English
    Publishing date 2024-04-06
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2950-3299
    ISSN 2950-3299
    DOI 10.1016/j.omton.2024.200800
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: The Protective Effect of Crocin on Rat Bone Marrow Mesenchymal Stem Cells Exposed to Aluminum Chloride as an Endocrine Disruptor.

    Amini, Elaheh / Baharvand, Zahra / Niknejad, Azadeh / Tabari, Yasaman / Shemshadi, Sahel

    Avicenna journal of medical biotechnology

    2023  Volume 16, Issue 2, Page(s) 81–87

    Abstract: Background: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and proliferate which gives them healing properties in various tissues. Aluminium chloride (AlCl: Methods: To investigate whether crocin can reduce AlCl: Results: The results ...

    Abstract Background: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and proliferate which gives them healing properties in various tissues. Aluminium chloride (AlCl
    Methods: To investigate whether crocin can reduce AlCl
    Results: The results showed that AlCl
    Conclusion: Overall, the study suggests that crocin can protect BM-MSCs from AlCl
    Language English
    Publishing date 2023-08-25
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2520683-7
    ISSN 2008-4625 ; 2008-2835
    ISSN (online) 2008-4625
    ISSN 2008-2835
    DOI 10.18502/ajmb.v16i2.14858
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Production of bioactive wheat puroindoline proteins in Nicotiana benthamiana using a virus-based transient expression system.

    Niknejad, Azadeh / Webster, Diane / Bhave, Mrinal

    Protein expression and purification

    2015  Volume 125, Page(s) 43–52

    Abstract: The emergence of antibiotic resistant pathogenic strains of bacteria has necessitated the development of novel antimicrobial agents. The puroindoline A and B (PINA and PINB) proteins of wheat, well-known for their roles in determining the important ... ...

    Abstract The emergence of antibiotic resistant pathogenic strains of bacteria has necessitated the development of novel antimicrobial agents. The puroindoline A and B (PINA and PINB) proteins of wheat, well-known for their roles in determining the important phenotype of grain texture, are also antimicrobial, making them attractive as natural bio-control agents. However, the biochemical basis of PIN functionality remains unclear due to limitations in expressing them at the required yield and purity and lack of accurate tertiary structure. This study focussed on rapid transient expression of PINs targeted to different subcellular compartments (chloroplast, apoplast, endoplasmic reticulum and cytosol) of Nicotiana benthamiana leaf cells using the deconstructed tobacco mosaic virus-based 'magnICON®' system. The expressed recombinant PINs were characterised by Western blot using the Durotest anti-friabilin antibody, enzyme-linked immunosorbent assays (ELISA) and antimicrobial activity tests. Maximum yield of the His-tagged PINs occurred when targeted to the chloroplast. Both PINs exhibited oligomeric and monomeric forms on gels, but Western blots with the widely used Durotest anti-friabilin antibody identified only oligomeric forms. Only the PINs purified by a hydrophobic interaction method exhibited monomeric forms with the anti-His tag antibody, indicating correct folding. Interestingly, the Durotest antibody did not bind to monomers, suggesting their epitope may be obscured. PINs purified by His-tag affinity purification under native conditions or by the hydrophobic method exhibited antimicrobial activities. The successful in planta expression and optimisation of purification will enable future studies to examine the detailed structure of the PINs and explore novel bio-control applications in health, food and/or agriculture.
    MeSH term(s) Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacology ; Bacteria/drug effects ; Blotting, Western ; Cell Line ; Chloroplasts/metabolism ; Chromatography, Affinity ; Escherichia coli ; Fungi/drug effects ; Gene Expression Regulation, Plant ; Hydrophobic and Hydrophilic Interactions ; Phenotype ; Plant Leaves/cytology ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Plant Proteins/isolation & purification ; Plant Proteins/pharmacology ; Protein Multimerization ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Nicotiana/cytology ; Nicotiana/genetics ; Nicotiana/metabolism ; Tobacco Mosaic Virus/genetics ; Triticum/genetics ; Triticum/metabolism
    Chemical Substances Anti-Infective Agents ; Plant Proteins ; Recombinant Proteins ; puroindoline protein, Triticum aestivum
    Language English
    Publishing date 2015-09-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1055455-5
    ISSN 1096-0279 ; 1046-5928
    ISSN (online) 1096-0279
    ISSN 1046-5928
    DOI 10.1016/j.pep.2015.09.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3084: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top