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Article: Percutaneous Radiofrequency Disc Decompression: A Study of 27 Patients.

Živković, N / Ruetten, S / Tasić, G / Janicijević, A / Jelena, K / Nikola, J / Andjelković, I / Jovanović, S

2023 Volume 26, Issue 12, Page(s) 1850–1853

Abstract: Background: Percutaneous radiofrequency nucleoplasty is a true minimally invasive technique for treatment for radiculopathy caused by contained disc protrusions. This minimally invasive procedure uses controlled thermoablation for reducing the ... ...

Abstract	Background: Percutaneous radiofrequency nucleoplasty is a true minimally invasive technique for treatment for radiculopathy caused by contained disc protrusions. This minimally invasive procedure uses controlled thermoablation for reducing the intervertebral disc and decompressing the lumbar nerve root. Material and Methods: Our study is a prospective analysis of 27 patients aged from 30 to 64 years with lumbar disc protrusion who were treated with percutaneous radiofrequency disc decompression (PRFD) between May 2018 and May 2019. Clinical follow-up was reported at 1 month, 3 months, and 6 months. The outcomes were assessed using a visual analog scale (VAS) and MacNab score. Results: Of the 27 patients, 14 were female and 13 were male. Their mean age was 53 ± 2 years. In all 27 patients, percutaneous radiofrequency nucleotomy was performed. An excellent outcome as reflected by MacNab score was observed in 17 patients (63%), a good outcome in 8 patients (29.7%), and a poor outcome in 2 patients (7.3%). Prior to treatment, the average back and leg VAS scores were 7.95 and 7.82, respectively. At sixth month follow-up, the back and leg VAS scores were reduced to 3.17 and 3.04, respectively. Patients with a poor outcome developed early recurrent disc prolapse and required endoscopic discectomy. Conclusion: PRFD is a safe and effective treatment of contained disc protrusion. PRFD is a good alternative to surgery. These procedures significantly increase quality of life in patients with lumbar radiculopathy.
MeSH term(s)	Humans ; Male ; Female ; Middle Aged ; Intervertebral Disc Displacement/surgery ; Radiculopathy/surgery ; Quality of Life ; Diskectomy/adverse effects ; Diskectomy/methods ; Endoscopy/methods ; Treatment Outcome ; Decompression/adverse effects ; Retrospective Studies
Language	English
Publishing date	2023-12-29
Publishing country	India
Document type	Journal Article
ZDB-ID	1458330-6
ISSN	1119-3077
ISSN	1119-3077
DOI	10.4103/njcp.njcp_375_23
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Article ; Online: Process management and integrated management systems

Jovanović Duško R. / Milenković Nikola J. / Dumonjić Bojan M.

Војно дело, Vol 69, Iss 6, Pp 424-

2017 Volume 435

Abstract: The growing interest in the topics of management systems, as well as the concept of their integration in different organizational systems, has led to the need to clarify the basic concepts and concepts related to them. This paper will explain the basic ... ...

Abstract	The growing interest in the topics of management systems, as well as the concept of their integration in different organizational systems, has led to the need to clarify the basic concepts and concepts related to them. This paper will explain the basic concepts, the relationship and significance of the process, as well as the place and importance of process management in integrated management systems.
Keywords	process ; control ; management ; integrated ; Military Science ; U
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Ministry of Defence of Serbia - Military Publishing House, Belgrade
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Identification of stakeholders

Jovanović Duško R. / Milenković Nikola J. / Dumonjić Bojan M.

Војно дело, Vol 69, Iss 7, Pp 384-

Methods and approaches

2017 Volume 395

Abstract: In contemporary business conditions, identification of stakeholders appears as a necessity. This paper explains the concept of a stakeholder, an entity that is gaining increasing importance for an organization. Moreover, the methods and approaches ... ...

Abstract	In contemporary business conditions, identification of stakeholders appears as a necessity. This paper explains the concept of a stakeholder, an entity that is gaining increasing importance for an organization. Moreover, the methods and approaches developed for the purpose of identifying stakeholders, as well as management system integration that respects identification of stakeholders and their requirements are listed.
Keywords	stakeholder ; identification ; integrated management system ; Military Science ; U
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Ministry of Defence of Serbia - Military Publishing House, Belgrade
Document type	Article ; Online
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Article ; Online: A new technique of arsenic determination based on electrolytic arsine generation and atomic absorption spectroscopy

Sevaljevic Mirjana M. / Mentus Slavko V. / Marjanovic Nikola J.

Journal of the Serbian Chemical Society, Vol 66, Iss 6, Pp 419-

2016 Volume 427

Abstract: A method for the determination of traces of arsenic in food has been developed based on electrolytic arsine generation and atomic absorption spectroscopy detection. The essence of the method is the addition of copper and tin salts to the electrolyte ... ...

Abstract	A method for the determination of traces of arsenic in food has been developed based on electrolytic arsine generation and atomic absorption spectroscopy detection. The essence of the method is the addition of copper and tin salts to the electrolyte solution, enabling a fast and effective arsine evolution. The advantage of this method lies in the fact that it enables the simultaneous concentration of heavy metals, such as cadmium and lead, on cathode. The applicability of the method was illustrated by determining the arsenic content in some food samples based on vegetable oils and by comparing the results with those obtained by the classical technique of chemical hydriding with sodium tetrahydroborate.
Keywords	arsine generation ; atomic absorption spectroscopy ; electrolysis ; trace determination ; diffusion rate constants ; Chemistry ; QD1-999 ; Science ; Q
Language	English
Publishing date	2016-10-01T00:00:00Z
Publisher	Serbian Chemical Society
Document type	Article ; Online
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Article ; Online: Defective oogenesis in mice with pristane-induced model of systemic lupus.

Delimitreva, Stefka M / Boneva, Gabriela V / Chakarova, Irina V / Hadzhinesheva, Valentina P / Zhivkova, Ralitsa S / Markova, Maya D / Nikolova, Venera P / Kolarov, Anton I / Mladenov, Nikola J / Bradyanova, Silviya L / Tchorbanov, Andrey I

Journal of reproductive immunology

2021 Volume 148, Page(s) 103370

Abstract: Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease characterized by generation of autoantibodies and severe damage of various organs. The hormonal changes associated with pregnancy and especially estrogen might lead to damage of ... ...

Abstract	Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease characterized by generation of autoantibodies and severe damage of various organs. The hormonal changes associated with pregnancy and especially estrogen might lead to damage of reproductive function and ovarian quality. We employed a pristane-induced lupus model of Balb/c mice which resembles human lupus in an attempt to follow oogenesis disruption during the disease progression. The integrity of cytoskeletal and chromatin structures was estimated in oocytes derived by hormonally stimulated ovulation in lupus mice and the results were compared with those from healthy mice. Chromatin, tubulin and actin structures in oocytes were detected by Hoechst 33258, anti-alpha-tubulin antibody and rhodamine-labeled phalloidin, respectively. All available meiotic spindles were analyzed - in immature (metaphase I) and mature oocytes (metaphase II). The total number of mature oocytes obtained from lupus mice was lower compared to healthy controls. The maturation rate was 9.8 % for lupus mice, 12.7 % for 7-month old controls, and 14.3 % for the young control mice (4 weeks old). Another major difference between the studied groups was the higher percentage of defective metaphase I spindles registered in oocytes derived from lupus mice (60 % normal spindles), while for the young and older controls this proportion was 86 % and 81 %, respectively. No such difference was registered for metaphase II spindles. For both metaphase I and metaphase II oocytes, the proportions of normal actin cap and chromosomal condensation were similar between the experimental groups.
MeSH term(s)	Animals ; Cell Differentiation ; Cells, Cultured ; Disease Models, Animal ; Female ; Humans ; Lupus Erythematosus, Systemic/physiopathology ; Metaphase ; Mice ; Mice, Inbred BALB C ; Oogenesis/physiology ; Pregnancy ; Terpenes
Chemical Substances	Terpenes ; pristane (26HZV48DT1)
Language	English
Publishing date	2021-09-02
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	424421-7
ISSN	1872-7603 ; 0165-0378
ISSN (online)	1872-7603
ISSN	0165-0378
DOI	10.1016/j.jri.2021.103370
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Article ; Online: Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results

Nešić Ksenija D. / Pisinov Boris P. / Jakšić Sandra M. / Tasić Aleksandra М. / Savić Božidar M. / Pavlović Nikola J.

Zbornik Matice Srpske za Prirodne Nauke, Vol 2017, Iss 133, Pp 79-

2017 Volume 93

Abstract: Different analytical techniques for the detection of mycotoxins have been developed in order to control the levels of mycotoxins in food and feed. Conventional analytical methods for mycotoxin determination are involving techniques such as thinlayer ... ...

Abstract	Different analytical techniques for the detection of mycotoxins have been developed in order to control the levels of mycotoxins in food and feed. Conventional analytical methods for mycotoxin determination are involving techniques such as thinlayer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatography (GC). Also, rapid methods for mycotoxin analysis have become increasingly important. Enzyme-linked immuno-sorbent assay (ELISA) is one of the most common rapid methods for determination of these natural contaminants. The aim of this study was to provide a comparison between two different methods of analysis (HPLC and ELISA) for the detection of different mycotoxins using data that originate from commercial proficiency tests. Based on the statistical evaluation of the results for both methods, in three proficiency tests for various mycotoxins (aflatoxins, ochratoxin and zearalenone), it could be concluded that both techniques can equally be used, although ELISA is considered to be the screening one. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46009]
Keywords	ELISA ; HPLC ; food and feed ; mycotoxins ; Science (General) ; Q1-390
Subject code	540
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Matica srpska
Document type	Article ; Online
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Article: Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results

Nešić Ksenija D. / Pisinov Boris P. / Jakšić Sandra M. / Tasić Aleksandra М / Savić Božidar M. / Pavlović Nikola J.

Zbornik Matice srpske za prirodne nauke. 2017, v. 2017, no. 133

2017

Abstract	Different analytical techniques for the detection of mycotoxins have been developed in order to control the levels of mycotoxins in food and feed. Conventional analytical methods for mycotoxin determination are involving techniques such as thinlayer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatography (GC). Also, rapid methods for mycotoxin analysis have become increasingly important. Enzyme-linked immuno-sorbent assay (ELISA) is one of the most common rapid methods for determination of these natural contaminants. The aim of this study was to provide a comparison between two different methods of analysis (HPLC and ELISA) for the detection of different mycotoxins using data that originate from commercial proficiency tests. Based on the statistical evaluation of the results for both methods, in three proficiency tests for various mycotoxins (aflatoxins, ochratoxin and zearalenone), it could be concluded that both techniques can equally be used, although ELISA is considered to be the screening one. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46009]
Keywords	aflatoxins ; education ; enzyme-linked immunosorbent assay ; gas chromatography ; high performance liquid chromatography ; ochratoxins ; zearalenone
Language	English
Size	p. 79-93.
Publishing place	Matica srpska
Document type	Article
ZDB-ID	2603457-8
ISSN	2406-0828 ; 0352-4906
ISSN (online)	2406-0828
ISSN	0352-4906
DOI	10.2298/ZMSPN1733079N
Database	NAL-Catalogue (AGRICOLA)

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Article: Atomic force microscopy measurements of intermolecular binding forces.

Misevic, Gradimir N / Karamanos, Yannis / Misevic, Nikola J

Methods in molecular biology (Clifton, N.J.)

2009 Volume 522, Page(s) 143–150

Abstract: Atomic force microscopy (AFM) measurements of intermolecular binding strength between a single pair of complementary cell adhesion molecules in physiological solutions provided the first quantitative evidence for their cohesive function. This novel AFM- ... ...

Abstract	Atomic force microscopy (AFM) measurements of intermolecular binding strength between a single pair of complementary cell adhesion molecules in physiological solutions provided the first quantitative evidence for their cohesive function. This novel AFM-based nanobiotechnology opens a molecular mechanic approach for studying structure- to function-related properties of any type of individual biological macromolecules. The presented example of Porifera cell adhesion glyconectin proteoglycans showed that homotypic carbohydrate to carbohydrate interactions between two primordial proteoglycans can hold the weight of 1,600 cells. Thus, glyconectin type carbohydrates, as the most peripheral cell surface molecules of sponges (today's simplest living Metazoa), are proposed to be the primary cell adhesive molecules essential for the evolution of the multicellularity.
MeSH term(s)	Biotechnology ; Carbohydrates/chemistry ; Microscopy, Atomic Force/methods ; Microscopy, Electron, Scanning Transmission ; Nanotechnology
Chemical Substances	Carbohydrates
Language	English
Publishing date	2009
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1064-3745
ISSN	1064-3745
DOI	10.1007/978-1-59745-413-1_8
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Article ; Online: The formate/nitrite transporter family of anion channels.

Lü, Wei / Du, Juan / Schwarzer, Nikola J / Wacker, Tobias / Andrade, Susana L A / Einsle, Oliver

Biological chemistry

2013 Volume 394, Issue 6, Page(s) 715–727

Abstract: The formate/nitrite transporter (FNT) family of integral membrane proteins comprises pentameric channels for monovalent anions that exhibit a broad specificity for small anions such as chloride, the physiological cargo molecules formate, nitrite, and ... ...

Abstract	The formate/nitrite transporter (FNT) family of integral membrane proteins comprises pentameric channels for monovalent anions that exhibit a broad specificity for small anions such as chloride, the physiological cargo molecules formate, nitrite, and hydrosulfide, and also larger organic acids. Three-dimensional structures are available for the three known subtypes, FocA, NirC, and HSC, which reveal remarkable evolutionary optimizations for the respective physiological context of the channels. FNT channels share a conserved translocation pathway in each protomer, with a central hydrophobic cavity that is separated from both sides of the membrane by a narrow constriction. A single protonable residue, a histidine, plays a key role by transiently protonating the transported anion to allow an uncharged species to pass the hydrophobic barrier. Further selectivity is reached through variations in the electrostatic surface potential of the proteins, priming the formate channel FocA for anion export, whereas NirC and HSC should work bidirectionally. Electrophysiological studies have shown that a broad variety of monovalent anions can be transported, and in the case of FocA, these match exactly the products of mixed-acid fermentation, the predominant metabolic pathway for most enterobacterial species.
MeSH term(s)	Animals ; Anions/metabolism ; Formates/metabolism ; Humans ; Ion Channels/chemistry ; Ion Channels/metabolism ; Ion Transport ; Models, Molecular ; Nitrites/metabolism
Chemical Substances	Anions ; Formates ; Ion Channels ; Nitrites
Language	English
Publishing date	2013-06
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1334659-3
ISSN	1437-4315 ; 1431-6730 ; 1432-0355
ISSN (online)	1437-4315
ISSN	1431-6730 ; 1432-0355
DOI	10.1515/hsz-2012-0339
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Article ; Online: Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage.

Requejo, Raquel / Hurd, Thomas R / Costa, Nikola J / Murphy, Michael P

The FEBS journal

2010 Volume 277, Issue 6, Page(s) 1465–1480

Abstract: Cysteine plays a number of important roles in protecting the cell from oxidative damage through its thiol functional group. These defensive functions are generally considered to be carried out by the low molecular weight thiol glutathione and by cysteine ...

Abstract	Cysteine plays a number of important roles in protecting the cell from oxidative damage through its thiol functional group. These defensive functions are generally considered to be carried out by the low molecular weight thiol glutathione and by cysteine residues in the active sites of proteins such as thioredoxin and peroxiredoxin. In addition, there are thiols exposed on protein surfaces that are not directly involved with protein function, although they can interact with the intracellular environment. In the present study, in subcellular fractions prepared from rat liver or heart, we show that the quantitatively dominant free thiols are those of cysteine residues exposed on protein surfaces and not those carried by glutathione. Within the mitochondrial matrix, the concentration of exposed protein thiols is 60-90 mm, which is approximately 26-fold higher than the glutathione concentration in that compartment. This suggests that exposed protein thiols are of greater importance than glutathione for nonenzyme catalysed reactions of thiols with reactive oxygen and nitrogen species and with electrophiles within the cell. One such antioxidant role for exposed protein thiols may be to prevent protein oxidative damage. In the present study, in mitochondrial membranes and in complex I, we show that exposed protein thiols protect against tyrosine nitration and protein dysfunction caused by peroxynitrite. Therefore, exposed protein thiols are the dominant free thiol within the cell and may play a critical role in intracellular antioxidant defences against oxidative damage.
MeSH term(s)	Animals ; Cells, Cultured ; Cysteine/metabolism ; Glutathione/chemistry ; Glutathione/metabolism ; Membrane Proteins/physiology ; Mitochondria, Heart/physiology ; Mitochondria, Liver/physiology ; Oxidative Stress ; Rats ; Sulfhydryl Compounds/chemistry ; Sulfhydryl Compounds/metabolism
Chemical Substances	Membrane Proteins ; Sulfhydryl Compounds ; Glutathione (GAN16C9B8O) ; Cysteine (K848JZ4886)
Language	English
Publishing date	2010-02-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/j.1742-4658.2010.07576.x
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