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  1. AU="Nikola Strempel"
  2. AU="Ning, Yuye"
  3. AU="Li, Huiyu"
  4. AU="Pannala, Ananth S"
  5. AU="Noda, K."
  6. AU="Almeida, Carolina Aparecida Faria"
  7. AU=Khoshakhlagh Arezou
  8. AU="Sofija Glamočlija"
  9. AU="Fleming, Sherry D"
  10. AU="Minkina, Tatiana M"
  11. AU="Fonseca, Danielle"
  12. AU="Maximilian, Carmen-Rodica"
  13. AU="Heuer, Lauren B"
  14. AU="Pan, Judy"
  15. AU="Doro, M."
  16. AU="Navarro-Zapata, Alfonso"
  17. AU="Martin, Emanuel H"
  18. AU="Biswas, Arnab"
  19. AU="Kurt Pfister"
  20. AU="Stefano Brignola"
  21. AU="Nierzwicki, Łukasz"
  22. AU="Benvin, Iva"
  23. AU="Sardesai, S. C."
  24. AU="Aldrees, Rana"

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  1. Artikel ; Online: Oxidative Stress Response in Pseudomonas aeruginosa

    Waleska Stephanie da Cruz Nizer / Vasily Inkovskiy / Zoya Versey / Nikola Strempel / Edana Cassol / Joerg Overhage

    Pathogens, Vol 10, Iss 1187, p

    2021  Band 1187

    Abstract: Pseudomonas aeruginosa is a Gram-negative environmental and human opportunistic pathogen highly adapted to many different environmental conditions. It can cause a wide range of serious infections, including wounds, lungs, the urinary tract, and systemic ... ...

    Abstract Pseudomonas aeruginosa is a Gram-negative environmental and human opportunistic pathogen highly adapted to many different environmental conditions. It can cause a wide range of serious infections, including wounds, lungs, the urinary tract, and systemic infections. The high versatility and pathogenicity of this bacterium is attributed to its genomic complexity, the expression of several virulence factors, and its intrinsic resistance to various antimicrobials. However, to thrive and establish infection, P. aeruginosa must overcome several barriers. One of these barriers is the presence of oxidizing agents (e.g., hydrogen peroxide, superoxide, and hypochlorous acid) produced by the host immune system or that are commonly used as disinfectants in a variety of different environments including hospitals. These agents damage several cellular molecules and can cause cell death. Therefore, bacteria adapt to these harsh conditions by altering gene expression and eliciting several stress responses to survive under oxidative stress. Here, we used PubMed to evaluate the current knowledge on the oxidative stress responses adopted by P. aeruginosa . We will describe the genes that are often differently expressed under oxidative stress conditions, the pathways and proteins employed to sense and respond to oxidative stress, and how these changes in gene expression influence pathogenicity and the virulence of P. aeruginosa . Understanding these responses and changes in gene expression is critical to controlling bacterial pathogenicity and developing new therapeutic agents.
    Schlagwörter Pseudomonas aeruginosa ; oxidative stress ; oxidative stress response ; reactive oxygen species ; reactive chlorine species ; antimicrobial resistance ; Medicine ; R
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2021-09-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Human CAP cells represent a novel source for functional, miRNA-loaded exosome production.

    Nikolas Zeh / Helga Schneider / Sven Mathias / Nadja Raab / Michael Kleemann / Sabine Schmidt-Hertel / Benjamin Weis / Silke Wissing / Nikola Strempel / René Handrick / Kerstin Otte

    PLoS ONE, Vol 14, Iss 8, p e

    2019  Band 0221679

    Abstract: Exosomes represent a promising delivery tool for nucleic acid-based pharmaceuticals. They are highly suitable for transporting therapeutic miRNAs to tumor cells, due to their natural membrane components. Further, exosomes are capable of effectively ... ...

    Abstract Exosomes represent a promising delivery tool for nucleic acid-based pharmaceuticals. They are highly suitable for transporting therapeutic miRNAs to tumor cells, due to their natural membrane components. Further, exosomes are capable of effectively protecting nucleic acids against ribonucleases and enable the delivery of their content through cell membranes. However, no suitable production host for miRNA containing exosomes of non-tumorigenic origin has yet been identified. In this study we engineered an immortalised human amniocyte cell line (CAP® cells), whose exosomes were enriched and characterised. The cell line modifications not only enabled the production of GFP-labelled but also pro-apoptotic miRNA containing exosomes without negative influence on host cell growth. Furthermore, we demonstrated that pro-apoptotic miRNA containing CAP exosomes are taken up by ovarian cancer cells. Strikingly, delivery of functional exosomal miRNA led to downregulation of several reported target genes in the treated tumor cells. In summary, we revealed CAP cells of non-tumorigenic origin as a novel and efficient exosome production host with the potential to produce functional miRNA-loaded exosomes.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 500
    Sprache Englisch
    Erscheinungsdatum 2019-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Human host defense peptide LL-37 stimulates virulence factor production and adaptive resistance in Pseudomonas aeruginosa.

    Nikola Strempel / Anke Neidig / Michael Nusser / Robert Geffers / Julien Vieillard / Olivier Lesouhaitier / Gerald Brenner-Weiss / Joerg Overhage

    PLoS ONE, Vol 8, Iss 12, p e

    2013  Band 82240

    Abstract: A multitude of different virulence factors as well as the ability to rapidly adapt to adverse environmental conditions are important features for the high pathogenicity of Pseudomonas aeruginosa. Both virulence and adaptive resistance are tightly ... ...

    Abstract A multitude of different virulence factors as well as the ability to rapidly adapt to adverse environmental conditions are important features for the high pathogenicity of Pseudomonas aeruginosa. Both virulence and adaptive resistance are tightly controlled by a complex regulatory network and respond to external stimuli, such as host signals or antibiotic stress, in a highly specific manner. Here, we demonstrate that physiological concentrations of the human host defense peptide LL-37 promote virulence factor production as well as an adaptive resistance against fluoroquinolone and aminoglycoside antibiotics in P. aeruginosa PAO1. Microarray analyses of P. aeruginosa cells exposed to LL-37 revealed an upregulation of gene clusters involved in the production of quorum sensing molecules and secreted virulence factors (PQS, phenazine, hydrogen cyanide (HCN), elastase and rhamnolipids) and in lipopolysaccharide (LPS) modification as well as an induction of genes encoding multidrug efflux pumps MexCD-OprJ and MexGHI-OpmD. Accordingly, we detected significantly elevated levels of toxic metabolites and proteases in bacterial supernatants after LL-37 treatment. Pre-incubation of bacteria with LL-37 for 2 h led to a decreased susceptibility towards gentamicin and ciprofloxacin. Quantitative Realtime PCR results using a PAO1-pqsE mutant strain present evidence that the quinolone response protein and virulence regulator PqsE may be implicated in the regulation of the observed phenotype in response to LL-37. Further experiments with synthetic cationic antimicrobial peptides IDR-1018, 1037 and HHC-36 showed no induction of pqsE expression, suggesting a new role of PqsE as highly specific host stress sensor.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2013-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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