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  1. AU="Nikula-Ijäs, Pirjo"
  2. AU="Germain, Elsa"
  3. AU="Veltink, Peter"
  4. AU=Subbarayan Karthikeyan
  5. AU="Bellomo, Chiara"
  6. AU="Jeon, Soo Min"
  7. AU="Rotaru, Mihaela"
  8. AU="Hakami, Mohammed Ageeli"
  9. AU="Garduño, Eugenio"
  10. AU="Kumar Vijay, Ajay"
  11. AU="Concheiro, Marta"
  12. AU="Kang, Ji-Hoon"
  13. AU="González-Gómez, Julio César"
  14. AU="Eisinger, Felix"
  15. AU="van Arkel, Gijs H J"
  16. AU="Dukkipati, Haripriya"
  17. AU="Mansoor, Farheen"
  18. AU="Stanton, Clive"
  19. AU=Herholz K AU=Herholz K
  20. AU="Marichal, Axel"
  21. AU="Camon, Ana M"
  22. AU="Randall, Michael D"

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  1. Artikel: Antimicrobial and Anti-Inflammatory Lingonberry Mouthwash—A Clinical Pilot Study in the Oral Cavity

    Pärnänen, Pirjo / Nikula-Ijäs, Pirjo / Sorsa, Timo

    Microorganisms. 2019 Sept. 08, v. 7, no. 9

    2019  

    Abstract: Fermented lingonberry juice was designed to be used as a mouthwash. Our aim was to study the antimicrobial and anti-inflammatory effects of the mouthwash in the oral cavity. A clinical study of 30 adult participants was performed. A total of 20 ... ...

    Abstract Fermented lingonberry juice was designed to be used as a mouthwash. Our aim was to study the antimicrobial and anti-inflammatory effects of the mouthwash in the oral cavity. A clinical study of 30 adult participants was performed. A total of 20 participants used 10 mL of the mouthwash twice daily for two weeks and 10 participants used 20 mL twice daily for one week. Streptococcus mutans, Candida and Lactobacilli were cultivated at the beginning, after the mouthwash period and after a washout period. At the same timepoints an additional oral mouthrinse was collected for chair-side/point-of-care (POC)-PerioSafe®/OraLyzer® aMMP-8 quantitative on-line evaluation, and an oral clinical investigation was performed. Mean Streptococcus mutans and Candida counts, visible plaque index (VPI) and bleeding on probing (BOP) were reduced, and Lactobacilli counts increased during the lingonberry mouthwash period. The aMMP-8 mouthrinses showed reduced values in both test groups when compared to the startpoint. The mouthrinse aMMP-8 reduction correlated with the reductions in microbial counts, VPI and BOP. Based on the results, fermented lingonberry juice seems a promising aid in oral homecare, diminishing the microbial and related proinflammatory burden by balancing the oral microbial flora and gradually lowering the inflammatory load in the oral cavity.
    Schlagwörter Candida ; Lactobacillus ; Streptococcus mutans ; Vaccinium vitis-idaea ; adults ; anti-inflammatory activity ; fruit juices ; hemorrhage ; microorganisms ; mouth ; point-of-care systems
    Sprache Englisch
    Erscheinungsverlauf 2019-0908
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms7090331
    Datenquelle NAL Katalog (AGRICOLA)

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  2. Artikel: Antimicrobial and Anti-inflammatory Lingonberry Mouthwash - A Clinical Pilot Study in the Oral Cavity.

    Pärnänen, Pirjo / Nikula-Ijäs, Pirjo / Sorsa, Timo

    Microorganisms

    2019  Band 7, Heft 9

    Abstract: Fermented lingonberry juice was designed to be used as a mouthwash. Our aim was to study the antimicrobial and anti-inflammatory effects of the mouthwash in the oral cavity. A clinical study of 30 adult participants was performed. A total of 20 ... ...

    Abstract Fermented lingonberry juice was designed to be used as a mouthwash. Our aim was to study the antimicrobial and anti-inflammatory effects of the mouthwash in the oral cavity. A clinical study of 30 adult participants was performed. A total of 20 participants used 10 mL of the mouthwash twice daily for two weeks and 10 participants used 20 mL twice daily for one week. Streptococcus mutans, Candida and Lactobacilli were cultivated at the beginning, after the mouthwash period and after a washout period. At the same timepoints an additional oral mouthrinse was collected for chair-side/point-of-care (POC)-PerioSafe
    Sprache Englisch
    Erscheinungsdatum 2019-09-08
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms7090331
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Isolation, characterization and regulation of moonlighting proteases from Candida glabrata cell wall.

    Pärnänen, Pirjo / Sorsa, Timo / Tervahartiala, Taina / Nikula-Ijäs, Pirjo

    Microbial pathogenesis

    2020  Band 149, Seite(n) 104547

    Abstract: Candida glabrata (C. glabrata) cell wall proteins play a role in virulence and in initial host immune recognition and responses. We isolated and characterized C. glabrata cell wall proteases from a clinical hospital C. glabrata T-1638 blood isolate and ... ...

    Abstract Candida glabrata (C. glabrata) cell wall proteins play a role in virulence and in initial host immune recognition and responses. We isolated and characterized C. glabrata cell wall proteases from a clinical hospital C. glabrata T-1638 blood isolate and estimated the enzymatic activities and their ability to degrade gelatin and processing proMMP-8 and assess the regulation of these proteases with salt treatment, mercaptoethanol and fermented lingonberry juice from Vaccinium vitis idaea L. The cell wall proteases were enzymatically released from the cell wall and beta- 1,3- bonded proteases were fractioned into 10-50 kDa and >50 kDa fractions with anionic DEAE-sepharose ion-exchange chromatography and gel filtration. Proteins were monitored and analyzed with MDPF- zymography, and five gelatinolytic bands were cut out from a parallel silver-stained gel for the LC- MS/MS analysis. The proteases lacked a signal sequence, indicating that they are moonlighting proteases. Human proMMP-8 activation assays were performed with both fractions and verified by western-immunoblot using aMMP-8 specific antibody. Inhibition of proMMP-8 conversion to the lower molecular active enzyme species were demonstrated with fermented lingonberry juice. The results indicate that moonlighting proteases may play a role in the virulence of C. glabrata.
    Mesh-Begriff(e) Candida glabrata ; Cell Wall ; Chromatography, High Pressure Liquid ; Humans ; Peptide Hydrolases ; Tandem Mass Spectrometry
    Chemische Substanzen Peptide Hydrolases (EC 3.4.-)
    Sprache Englisch
    Erscheinungsdatum 2020-09-30
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2020.104547
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Isolation, characterization and regulation of moonlighting proteases from Candida glabrata cell wall

    Pärnänen, Pirjo / Sorsa, Timo / Tervahartiala, Taina / Nikula-Ijäs, Pirjo

    Microbial pathogenesis. 2020 Dec., v. 149

    2020  

    Abstract: Candida glabrata (C. glabrata) cell wall proteins play a role in virulence and in initial host immune recognition and responses. We isolated and characterized C. glabrata cell wall proteases from a clinical hospital C. glabrata T-1638 blood isolate and ... ...

    Abstract Candida glabrata (C. glabrata) cell wall proteins play a role in virulence and in initial host immune recognition and responses. We isolated and characterized C. glabrata cell wall proteases from a clinical hospital C. glabrata T-1638 blood isolate and estimated the enzymatic activities and their ability to degrade gelatin and processing proMMP-8 and assess the regulation of these proteases with salt treatment, mercaptoethanol and fermented lingonberry juice from Vaccinium vitis idaea L. The cell wall proteases were enzymatically released from the cell wall and beta- 1,3- bonded proteases were fractioned into 10–50 kDa and >50 kDa fractions with anionic DEAE-sepharose ion-exchange chromatography and gel filtration. Proteins were monitored and analyzed with MDPF- zymography, and five gelatinolytic bands were cut out from a parallel silver-stained gel for the LC- MS/MS analysis. The proteases lacked a signal sequence, indicating that they are moonlighting proteases. Human proMMP-8 activation assays were performed with both fractions and verified by western-immunoblot using aMMP-8 specific antibody. Inhibition of proMMP-8 conversion to the lower molecular active enzyme species were demonstrated with fermented lingonberry juice. The results indicate that moonlighting proteases may play a role in the virulence of C. glabrata.
    Schlagwörter Candida glabrata ; Vaccinium vitis-idaea ; antibodies ; blood ; cell walls ; fermentation ; gel chromatography ; gelatin ; hospitals ; humans ; ion exchange chromatography ; pathogenesis ; proteinases ; signal peptide ; virulence
    Sprache Englisch
    Erscheinungsverlauf 2020-12
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2020.104547
    Datenquelle NAL Katalog (AGRICOLA)

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    Verfügbar in ZB MED Köln/Königswinter

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  5. Artikel: A novel Candida glabrata cell wall associated serine protease

    Pärnänen, Pirjo / Meurman, Jukka H / Nikula-Ijäs, Pirjo

    Biochemical and biophysical research communications. 2015 Feb. 20, v. 457

    2015  

    Abstract: We set out to identify the Candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. We studied a clinical C. glabrata strain T-1639, which was isolated from a ... ...

    Abstract We set out to identify the Candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. We studied a clinical C. glabrata strain T-1639, which was isolated from a patient from the Helsinki University Central Hospital. With non-reducing 2-D electrophoresis using parallel fluorogenic gels and mass spectrometry we identified a novel appr. 25 kDa (192 aa in length) cell wall located protease with an estimated pI of 7.6. The LC-MS/MS peptides matched with the ORF of predicted C. glabrata CBS138 cell wall protein Cwp1.2p/pI 7.7/212 aa (http://cbi.labri.fr/Génolevures/[NCBI access 49525604, UniProt access Q6FTZ7]), which is an ortholog to Saccharomyces cerevisiae cell wall protein Cwp1p (UniProt access P28319). The novel serine protease was released by β-1,3-glucanase treatment from the cell wall. In contrast to previous predictions this protease has an enzymatic function instead of being merely a structural cell wall protein. The protease showed gelatinolytic activity and was inhibited by PMSF, a known serine protease inhibitor. Further characterization of the protease may give insight to its role in infections caused by C. glabrata and possibly aid in the development of new kinds of antifungal drugs.
    Schlagwörter Candida glabrata ; Saccharomyces cerevisiae ; antifungal agents ; beta-glucanase ; candidiasis ; cell walls ; electrophoresis ; gels ; hospitals ; mass spectrometry ; open reading frames ; patients ; peptides ; prediction ; proteinase inhibitors ; serine proteinases ; structural proteins ; virulence
    Sprache Englisch
    Erscheinungsverlauf 2015-0220
    Umfang p. 676-680.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 205723-2
    ISSN 0006-291X ; 0006-291X
    ISSN (online) 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2015.01.047
    Datenquelle NAL Katalog (AGRICOLA)

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    Verfügbar in ZB MED Bonn

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  6. Artikel ; Online: A novel Candida glabrata cell wall associated serine protease.

    Pärnänen, Pirjo / Meurman, Jukka H / Nikula-Ijäs, Pirjo

    Biochemical and biophysical research communications

    2015  Band 457, Heft 4, Seite(n) 676–680

    Abstract: We set out to identify the Candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. We studied a clinical C. glabrata strain T-1639, which was isolated from a ... ...

    Abstract We set out to identify the Candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. We studied a clinical C. glabrata strain T-1639, which was isolated from a patient from the Helsinki University Central Hospital. With non-reducing 2-D electrophoresis using parallel fluorogenic gels and mass spectrometry we identified a novel appr. 25 kDa (192 aa in length) cell wall located protease with an estimated pI of 7.6. The LC-MS/MS peptides matched with the ORF of predicted C. glabrata CBS138 cell wall protein Cwp1.2p/pI 7.7/212 aa (http://cbi.labri.fr/Génolevures/[NCBI access 49525604, UniProt access Q6FTZ7]), which is an ortholog to Saccharomyces cerevisiae cell wall protein Cwp1p (UniProt access P28319). The novel serine protease was released by β-1,3-glucanase treatment from the cell wall. In contrast to previous predictions this protease has an enzymatic function instead of being merely a structural cell wall protein. The protease showed gelatinolytic activity and was inhibited by PMSF, a known serine protease inhibitor. Further characterization of the protease may give insight to its role in infections caused by C. glabrata and possibly aid in the development of new kinds of antifungal drugs.
    Mesh-Begriff(e) Amino Acid Sequence ; Candida glabrata/chemistry ; Candida glabrata/enzymology ; Candidiasis/microbiology ; Cell Wall/chemistry ; Cell Wall/enzymology ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Molecular Sequence Data ; Serine Proteases/analysis ; Tandem Mass Spectrometry
    Chemische Substanzen Serine Proteases (EC 3.4.-)
    Sprache Englisch
    Erscheinungsdatum 2015-02-20
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2015.01.047
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 116: Hefte anzeigen Standort:
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  7. Artikel: The Effect of Fermented Lingonberry Juice on

    Pärnänen, Pirjo / Nawaz, Ali / Sorsa, Timo / Meurman, Jukka / Nikula-Ijäs, Pirjo

    International journal of dentistry

    2017  Band 2017, Seite(n) 6185395

    Abstract: Lingonberries have a long traditional use in treating fungal infections on mucosal membranes, but very little is known about the exact antifungal mechanisms. We tested the effects of fermented lingonberry juice ... ...

    Abstract Lingonberries have a long traditional use in treating fungal infections on mucosal membranes, but very little is known about the exact antifungal mechanisms. We tested the effects of fermented lingonberry juice on
    Sprache Englisch
    Erscheinungsdatum 2017-03-30
    Erscheinungsland Egypt
    Dokumenttyp Journal Article
    ZDB-ID 2546524-7
    ISSN 1687-8736 ; 1687-8728
    ISSN (online) 1687-8736
    ISSN 1687-8728
    DOI 10.1155/2017/6185395
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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