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  1. Article ; Online: Earlier Activation of Interferon and Pro-Inflammatory Response Is Beneficial to Largemouth Bass ( Micropterus salmoides ) against Rhabdovirus Infection

    Runzhen He / Qianrong Liang / Ningyu Zhu / Xiaoye Zheng / Xiaoming Chen / Fan Zhou / Xueyan Ding

    Fishes, Vol 7, Iss 90, p

    2022  Volume 90

    Abstract: In order to understand the immune response of largemouth bass against Micropterus salmoides Rhabdovirus (MSRV), assisting disease resistance breeding, three largemouth bass breeding varieties Micropterus salmoides “Youlu No 3” (U3), “Youlu No 1” (U1) and ...

    Abstract In order to understand the immune response of largemouth bass against Micropterus salmoides Rhabdovirus (MSRV), assisting disease resistance breeding, three largemouth bass breeding varieties Micropterus salmoides “Youlu No 3” (U3), “Youlu No 1” (U1) and “Zhelu No 1” (P1) were challenged intraperitoneally with MSRV. Serum and tissues were sampled to study the changes in non-specific immune parameters, viral loads, and transcript levels of immune-related genes, and the cumulative mortality rate was recorded daily for 14 days. The results showed that the cumulative mortality rates in the U1, P1, and U3 groups were 6.66% ± 2.89%, 3.33% ± 2.89%, and 0, respectively. The higher mortality may attribute to the increased viral loads after infection in the liver (2.79 × 10 5 and 2.38 × 10 5 vs. 1.3 × 10 4 copies/mg), spleen (2.14 × 10 5 and 9.40 × 10 4 vs. 4.21 × 10 3 copies/mg), and kidney (3.59 × 10 4 and 8.40 × 10 3 vs. 2.42 × 10 3 copies/mg) in the U1 and P1 groups compared to the U3 group. The serum non-specific immune parameters (lysozyme, catalase, and acid phosphatase) were found to be increased significantly in the U3 group. In addition, the transcripts of interferon-related genes ( IFN-γ , IRF3 , and IRF7 ) and pro-inflammatory-related genes ( TNF-α and IL-1β ) exhibited up-regulation and peaked at 6 h post infection in the U3 group, which also exhibited up-regulation but peaked at 12–24 h post infection in the U1 and P1 groups. In conclusion, these findings indicate that earlier activation of interferon and pro-inflammatory response is beneficial to largemouth bass against MSRV infection. This experiment may provide an insight into understanding the immune mechanism of largemouth bass against MSRV infection and contributes to molecular-assisted selection.
    Keywords largemouth bass ; rhabdovirus ; non-specific immune ; interferon ; pro-inflammatory ; Biology (General) ; QH301-705.5 ; Genetics ; QH426-470
    Subject code 333
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Transcriptome Analysis of Immune Responses and Metabolic Regulations of Chinese Soft-Shelled Turtle ( Pelodiscus sinensis ) against Edwardsiella tarda Infection

    Qianrong Liang / Ningyu Zhu / Xiaoye Zheng / Xueyan Ding / Runzhen He / Hongsen Xu / Feifei Cao / Huili Xue / Fan Zhou / Tianlun Zheng

    Fishes, Vol 7, Iss 79, p

    2022  Volume 79

    Abstract: The Chinese soft-shelled turtle ( Pelodiscus sinensis ) is an important aquatic species in southern China that is threatened by many serious diseases. Edwardsiella tarda is one of the highly pathogenic bacteria that cause the white abdominal shell ... ...

    Abstract The Chinese soft-shelled turtle ( Pelodiscus sinensis ) is an important aquatic species in southern China that is threatened by many serious diseases. Edwardsiella tarda is one of the highly pathogenic bacteria that cause the white abdominal shell disease. Yet, little is known about the immune and metabolic responses of the Chinese soft-shelled turtle against E. tarda infection. In the paper, gene expression profiles in the turtle liver were obtained to study the immune responses and metabolic regulations induced by E. tarda infection using RNA sequencing. A total of 3908 differentially expressed unigenes between the experimental group and the control group were obtained by transcriptome analysis, among them, were the significantly upregulated unigenes and downregulated unigenes 2065 and 1922, respectively. Further annotation and analysis revealed that the DEGs were mainly enriched in complement and coagulation cascades, phagosome, and steroid hormone biosynthesis pathways, indicating that they were mainly associated with defense mechanisms in the turtle liver against E. tarda four days post infection. For the first time, we reported on the gene profile of anti- E. tarda response in the soft-shelled turtle, and our research might provide valuable data to support further study on anti- E. tarda defense mechanisms in turtles.
    Keywords Pelodiscus sinensis ; Edwardsiella tarda infection ; transcriptome analysis ; immune responses and metabolic regulations ; antibacterial defense mechanisms ; Biology (General) ; QH301-705.5 ; Genetics ; QH426-470
    Subject code 570
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Therapeutic development of group B Streptococcus meningitis by targeting a host cell signaling network involving EGFR

    Ningyu Zhu / Chengxian Zhang / Atish Prakash / Zheng Hou / Wei Liu / Weifeng She / Andrew Morris / Kwang Sik Kim

    EMBO Molecular Medicine, Vol 13, Iss 3, Pp n/a-n/a (2021)

    2021  

    Abstract: Abstract Group B Streptococcus (GBS) remains the most common Gram‐positive bacterium causing neonatal meningitis and GBS meningitis continues to be an important cause of mortality and morbidity. In this study, we showed that GBS penetration into the ... ...

    Abstract Abstract Group B Streptococcus (GBS) remains the most common Gram‐positive bacterium causing neonatal meningitis and GBS meningitis continues to be an important cause of mortality and morbidity. In this study, we showed that GBS penetration into the brain occurred initially in the meningeal and cortex capillaries, and exploits a defined host cell signaling network comprised of S1P2, EGFR, and CysLT1. GBS exploitation of such network in penetration of the blood–brain barrier was demonstrated by targeting S1P2, EGFR, and CysLT1 using pharmacological inhibition, gene knockout and knockdown cells, and gene knockout animals, as well as interrogation of the network (up‐ and downstream of each other). More importantly, counteracting such targets as a therapeutic adjunct to antibiotic therapy was beneficial in improving the outcome of animals with GBS meningitis. These findings indicate that investigating GBS penetration of the blood–brain barrier provides a novel approach for therapeutic development of GBS meningitis.
    Keywords blood–brain barrier ; CysLTs ; EGFR ; GBS meningitis ; S1P ; Medicine (General) ; R5-920 ; Genetics ; QH426-470
    Subject code 616
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Bacteria reduce flagellin synthesis to evade microglia-astrocyte-driven immunity in the brain

    Hao Sun / Xuehua Wan / Yu Fan / Peng Liu / Yajun Song / Ningyu Zhu / Zhifeng Duan / Qian Wang / Fang Chen / Changhong Zhou / Yangyang Zheng / Peng Ding / Fenxia Liu / Lu Feng / Kwang Sik Kim / Lei Wang

    Cell Reports, Vol 40, Iss 1, Pp 111033- (2022)

    2022  

    Abstract: Summary: The immune response of brain cells to invading bacteria in vivo and the mechanism used by pathogenic bacteria to escape brain immune surveillance remain largely unknown. It is believed that microglia eliminate bacteria by phagocytosis based on ... ...

    Abstract Summary: The immune response of brain cells to invading bacteria in vivo and the mechanism used by pathogenic bacteria to escape brain immune surveillance remain largely unknown. It is believed that microglia eliminate bacteria by phagocytosis based on in vitro data. Here we find that a small percentage of microglia in the brain engulf neonatal meningitis-causing Escherichia coli (NMEC), but more microglia are activated to produce tumor necrosis factor alpha (TNFα), which activates astrocytes to secrete complement component 3 (C3) involved in anti-bacterial activity. To evade anti-bacterial activity of the immune system, NMEC senses low concentration of threonine in cerebrospinal fluid (CSF) to down-modulate the expression of flagellin and reduce microglial TNFα and astrocyte C3 production. Our findings may help develop strategies for bacterial meningitis treatment.
    Keywords CP: Immunology ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: IMB-T130 targets 3-dehydroquinate synthase and inhibits Mycobacterium tuberculosis

    Ningyu Zhu / Xia Wang / Dongsheng Li / Yuan Lin / Xuefu You / Jiandong Jiang / Yanni Xu / Wei Jiang / Shuyi Si

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 9

    Abstract: Abstract The anti-tuberculosis (TB) agent IMB-T130 was speculated to be a multi-target compound. In this research, we found that IMB-T130 inhibits the catalytic activity of Mycobacterium tuberculosis 3-dehydroquinate synthase (MtDHQS), the enzyme in the ... ...

    Abstract Abstract The anti-tuberculosis (TB) agent IMB-T130 was speculated to be a multi-target compound. In this research, we found that IMB-T130 inhibits the catalytic activity of Mycobacterium tuberculosis 3-dehydroquinate synthase (MtDHQS), the enzyme in the second step of the shikimate pathway. IMB-T130 was identified as a selective inhibitor of MtDHQS with an IC50 value of 0.87 μg/mL. The interaction between the compound and protein was analysed by surface plasmon resonance and circular dichroism. Based on the in silico molecular docking results, the essential amino acids in the binding pocket were then confirmed by site-directed mutagenesis. Overexpression of DHQS reduced the antibacterial activity of IMB-T130 in cells, verifying that DHQS is the target of IMB-T130. IMB-T130 inhibited standard and drug-resistant M. tuberculosis strains by targeting DHQS. Our findings improve our understanding of MtDHQS and make it to be a potential target for new anti-TB drug discovery.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2018-11-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: First reported fatal Bacillus thuringiensis infections in Chinese soft-shelled turtles (Trionyx sinensis)

    Chen, Jianshun / Lei Kong / Ningyu Zhu / Tianlun Zheng / Xueyan Ding / Yijiang Bei / Zhongyang He

    Aquaculture. 2014 May 20, v. 428-429

    2014  

    Abstract: Recurrent outbreaks of an epizootic in Chinese soft-shelled turtles (Trionyx sinensis) in Zhejiang province, China in 2013, caused severe economic losses to turtle culture industry. This disease was characterized by listless swimming, craned or twisted ... ...

    Abstract Recurrent outbreaks of an epizootic in Chinese soft-shelled turtles (Trionyx sinensis) in Zhejiang province, China in 2013, caused severe economic losses to turtle culture industry. This disease was characterized by listless swimming, craned or twisted neck, hyperemic laryngeal mucosa, and intumescent and hyperemic intestinal canal accompanied by mass mortality. Eleven diseased turtles with typical signs were collected from three distinct representative farms. Eleven bacterial isolates were collected from the livers and kidneys of diseased turtles and identified as Bacillus thuringiensis based on Vitek biochemical test, observation of rhizoid growth and protein toxin crystal production, 16S rRNA gene sequence analysis and Bacillus group species-specific PCR. These isolates demonstrated highly pathogenic potential to juvenile Chinese soft-shelled turtles with natural-infection-like symptoms. To our knowledge, this is the first case of B. thuringiensis infection in Chinese soft-shelled turtles and even in aquatic animals.
    Keywords Bacillus thuringiensis ; epizootic diseases ; farms ; financial economics ; industry ; juveniles ; kidneys ; larynx ; liver ; mortality ; mucosa ; neck ; nucleotide sequences ; pathogenicity ; Pelodiscus sinensis ; polymerase chain reaction ; ribosomal RNA ; sequence analysis ; swimming ; turtle culture ; turtles ; China
    Language English
    Dates of publication 2014-0520
    Size p. 16-20.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2014.02.018
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: First case of soft shell disease in Chinese soft-shelled turtle (Trionyx sinens) associated with Aeromonas sobria–A. veronii complex

    Chen, Jianshun / Lei Kong / Ningyu Zhu / Tianlun Zheng / Xueyan Ding / Yijiang Bei / Zhongyang He

    Aquaculture. 2013 Aug. 25, v. 406-407

    2013  

    Abstract: Outbreaks of an epizootic in Chinese soft-shelled turtle (Trionyx sinensis) in Zhejiang province, China in 2012, caused severe economic losses to turtle culture industry. This disease was characterized by “soft dorsal shell” and “rugose abdominal shell” ... ...

    Abstract Outbreaks of an epizootic in Chinese soft-shelled turtle (Trionyx sinensis) in Zhejiang province, China in 2012, caused severe economic losses to turtle culture industry. This disease was characterized by “soft dorsal shell” and “rugose abdominal shell” accompanied by significantly slower growth rate. Six bacterial isolates were collected from livers and kidneys of diseased turtles, and identified as Aeromonas sobria–Aeromonas veronii complex based on morphological and biochemical characteristics and phylogenetic analysis derived from 16S rRNA and six housekeeping genes. These isolates demonstrated pathogenic potential to juvenile Chinese soft-shelled turtles. To our knowledge, this is the first report of large-scale soft shell disease in Chinese soft-shelled turtles.
    Keywords Aeromonas veronii ; genes ; industry ; kidneys ; liver ; Pelodiscus sinensis ; phylogeny ; ribosomal RNA ; Trionyx ; turtle culture ; turtles ; China
    Language English
    Dates of publication 2013-0825
    Size p. 62-67.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2013.05.006
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Proteomic study of Mycoplasma suis using the gel-based shotgun strategy

    Congli, Yuan / Xiaowei, Yang / Zhibiao, Yang / Ningyu, Zhu / Shenbo, Zheng / Peixing, Hou / Xiaofeng, Gu / Chengrong, Ye / Congbin, Yao / Jianguo, Zhu / Li, Cui / Xiuguo, Hua

    Veterinary microbiology. 2010 May 19, v. 142, no. 3-4

    2010  

    Abstract: Mycoplasma suis (M. suis) is an uncultivable pathogen that colonizes the surface of porcine erythrocytes. In the present study, gel-based LC-MS/MS was performed to analyze the proteomic composition of M. suis. We identified 191 proteins with two or more ... ...

    Abstract Mycoplasma suis (M. suis) is an uncultivable pathogen that colonizes the surface of porcine erythrocytes. In the present study, gel-based LC-MS/MS was performed to analyze the proteomic composition of M. suis. We identified 191 proteins with two or more peptides and additional 217 putative proteins with one peptide hit by cross-species searching the Mollicutes protein databases. Resultant proteins were classified based on their molecular functions. The majority of enzymes involved in central carbon metabolic pathways and nucleotide biosynthesis were confirmed by searching the KEGG database. The present study is the first report addressing the proteome of M. suis. Results indicate that the energy source of M. suis might depend mainly on glycometabolism.
    Keywords Mycoplasma suis ; animal pathogenic bacteria ; erythrocytes ; microbial colonization ; proteome ; proteomics ; microbial genetics ; bacterial proteins ; peptides ; enzymes ; biochemical pathways ; energy metabolism
    Language English
    Dates of publication 2010-0519
    Size p. 303-308.
    Publishing place Amsterdam; New York: Elsevier
    Document type Article
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2009.10.022
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  9. Article ; Online: The 1.8kb DNA fragment formerly confirmed as Mycoplasma suis (M. suis) specific was originated from the porcine genome.

    Congli, Yuan / Zhibiao, Yang / Ningyu, Zhu / Congbin, Yao / Xiaowei, Yang / Jianguo, Zhu / Li, Cui / Xiuguo, Hua

    Veterinary microbiology

    2009  Volume 138, Issue 1-2, Page(s) 197–8; author reply 199

    MeSH term(s) Animals ; Chromosome Mapping ; DNA/genetics ; DNA/isolation & purification ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Genome ; Mycoplasma/genetics ; Swine/genetics ; Swine/microbiology
    Chemical Substances DNA, Bacterial ; DNA (9007-49-2)
    Language English
    Publishing date 2009-07-02
    Publishing country Netherlands
    Document type Comment ; Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2009.02.010
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