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  1. AU="Nirja Thakur"
  2. AU="Jiang, Shimin"
  3. AU="Wu, Xue-Ying"
  4. AU="Carlos Augusto de Mattos"
  5. AU="Procopio, Francesco A"
  6. AU="Nagata, Kosei"
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  9. AU="Couto Souza, Paulo Henrique"
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  1. Artikel ; Online: Free spermidine evokes superoxide radicals that manifest toxicity

    Vineet Kumar / Rajesh Kumar Mishra / Debarghya Ghose / Arunima Kalita / Pulkit Dhiman / Anand Prakash / Nirja Thakur / Gopa Mitra / Vinod D Chaudhari / Amit Arora / Dipak Dutta

    eLife, Vol

    2022  Band 11

    Abstract: Spermidine and other polyamines alleviate oxidative stress, yet excess spermidine seems toxic to Escherichia coli unless it is neutralized by SpeG, an enzyme for the spermidine N-acetyl transferase function. Thus, wild-type E. coli can tolerate applied ... ...

    Abstract Spermidine and other polyamines alleviate oxidative stress, yet excess spermidine seems toxic to Escherichia coli unless it is neutralized by SpeG, an enzyme for the spermidine N-acetyl transferase function. Thus, wild-type E. coli can tolerate applied exogenous spermidine stress, but ΔspeG strain of E. coli fails to do that. Here, using different reactive oxygen species (ROS) probes and performing electron paramagnetic resonance spectroscopy, we provide evidence that although spermidine mitigates oxidative stress by lowering overall ROS levels, excess of it simultaneously triggers the production of superoxide radicals, thereby causing toxicity in the ΔspeG strain. Furthermore, performing microarray experiment and other biochemical assays, we show that the spermidine-induced superoxide anions affected redox balance and iron homeostasis. Finally, we demonstrate that while RNA-bound spermidine inhibits iron oxidation, free spermidine interacts and oxidizes the iron to evoke superoxide radicals directly. Therefore, we propose that the spermidine-induced superoxide generation is one of the major causes of spermidine toxicity in E. coli.
    Schlagwörter Escherichia coli ; SpeG ; spermidine toxicity ; superoxide radical ; iron metabolism ; oxidative stress ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 500
    Sprache Englisch
    Erscheinungsdatum 2022-04-01T00:00:00Z
    Verlag eLife Sciences Publications Ltd
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel: Biological Activity of Serine Protease Inhibitor Isolated from the Seeds of Phaseolus vulgaris

    Negi, Pratibha / Amarjit K. Nath / Nirja Thakur / Subash Chand

    Agricultural research. 2018 Sept., v. 7, no. 3

    2018  

    Abstract: Seed flour extract of local common bean cultivar Baspa inhibited gut proteases of Spodoptera litura Fab. The inhibitor protein was purified by ammonium sulphate precipitation and gel filtration chromatography. Freshly laid eggs of Pieris brassicae L. ... ...

    Abstract Seed flour extract of local common bean cultivar Baspa inhibited gut proteases of Spodoptera litura Fab. The inhibitor protein was purified by ammonium sulphate precipitation and gel filtration chromatography. Freshly laid eggs of Pieris brassicae L. treated with 220-μg inhibitor protein showed 89.60% reduction and delay in hatching by 2 days as compared to control. Feeding neonate larvae of P. brassicae on cabbage leaf discs coated with inhibitor protein (79.25 and 118.88 μg) for 6 days resulted in 50 and 89% larval mortality and reduction in larval growth by 20.77 and 48.47% as compared to untreated control. The treated larvae (118.88 μg) shifted on untreated leaf discs showed mortality after 3 days. However, the growth of treated larvae (79.25 μg) fed on untreated leaf discs further decreased to 45.17% showed delayed pupation, deformed pupa formation and no adult emergence in contrast to control larvae.
    Schlagwörter adults ; ammonium sulfate ; beans ; bioactive properties ; cabbage ; cultivars ; digestive system ; eggs ; enzyme inhibitors ; flour ; gel chromatography ; hatching ; larvae ; larval development ; leaves ; mortality ; neonates ; Phaseolus vulgaris ; Pieris brassicae ; proteinases ; pupae ; pupation ; seeds ; Spodoptera litura
    Sprache Englisch
    Erscheinungsverlauf 2018-09
    Umfang p. 265-270.
    Erscheinungsort Springer India
    Dokumenttyp Artikel
    ZDB-ID 2668414-7
    ISSN 2249-7218 ; 2249-720X
    ISSN (online) 2249-7218
    ISSN 2249-720X
    DOI 10.1007/s40003-018-0304-z
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel: Alpha-amylase inhibitor from local common bean selection: Effect on growth and development of Corcyra cephalonica

    Rani, Ashu / Amarjit K Nath / Nirja Thakur / Subhash Chand

    Journal of stored products research. 2018 Jan., v. 75

    2018  

    Abstract: Cultivars of common bean (Phaseolus vulgaris L.) from the Kalpa valley of the Kinnaur district were screened for alpha-amylase inhibitor activity against porcine pancreatic α-amylase and significant differences were found amongst them. The inhibitor ... ...

    Abstract Cultivars of common bean (Phaseolus vulgaris L.) from the Kalpa valley of the Kinnaur district were screened for alpha-amylase inhibitor activity against porcine pancreatic α-amylase and significant differences were found amongst them. The inhibitor protein was partially purified from Triloki cultivar by ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography. Inhibitor protein was non-competitive, heat labile and retained 55.51 per cent activity at 70 °C. It had two pH optima of 5.0 and 7.6. Inhibitor protein inhibited amylases of 3rd -4th instar larval extracts of Corcyra cephalonica Stainton, the rice moth. C. cephalonica larvae (3rd -4th instar) were fed on wheat flour mixed with 0.013 percent (w/w) of partially purified α-amylase inhibitor protein. The growth of larvae in the treatment was significantly reduced. Treated larvae showed 100 percent mortality after 11 days. In the untreated control, no larval mortality was observed and adults emerged after 45 days. The inhibitor protein was effective at very low concentrations and could be exploited for control of this grain storage pest.
    Schlagwörter adults ; alpha-amylase ; ammonium sulfate ; beans ; Corcyra cephalonica ; cultivars ; gel chromatography ; heat ; instars ; larvae ; mortality ; pH ; Phaseolus vulgaris ; storage pests ; swine ; wheat flour
    Sprache Englisch
    Erscheinungsverlauf 2018-01
    Umfang p. 35-37.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    ZDB-ID 410225-3
    ISSN 0022-474X
    ISSN 0022-474X
    DOI 10.1016/j.jspr.2017.10.009
    Datenquelle NAL Katalog (AGRICOLA)

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